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The map label for this gene is yngI [H]

Identifier: 119867848

GI number: 119867848

Start: 1919528

End: 1921084

Strand: Reverse

Name: yngI [H]

Synonym: Mkms_1809

Alternate gene names: 119867848

Gene position: 1921084-1919528 (Counterclockwise)

Preceding gene: 119867849

Following gene: 119867847

Centisome position: 33.48

GC content: 70.84

Gene sequence:

>1557_bases
ATGGCTGAGCCGCACACCGTCGCCGCCGTGCTGGGGCGCTGGGCGGTCGACCGCCCAGCGCACCCGCTGCTGATCTGCGA
CGCCGACCGCCTCGACTACGGGCAGGCCGAACGCCGGTCGGCTCGGCTGGCGCGCGGCCTGCTCGCCCTCGGTGCGGGCA
AGGGCACTCACGTCGGCCTGCTGTATCCCAACGGCGCGGAGTTCCTCGTCGGCATGTTGGCCGCGGCCCGCATCGGTGCG
GTGGTGGTGCCGTTCAGCACCTTCGCCACGGCCCGCGAACTCGCAGAGCAGTTGCGCCACAGCGACACCGAGATCCTGTT
GTCGGCCGGATCGTTCCGCTCCCATGACTACGCCTCGCGGCTACGCGAGGTACTCGGGGGTGACATCCCCGCCGGGGAAC
GCCTCTACTGCGCCGCGGTCCCCCACCTGCGCCACGTGTTCGTCGACCGGCATCCCGACGGCGGCGTCGATCCCGCCCTG
CTGACCGCGGTCGAGGACGACGTCGACGGCAGCGACCCGTTGACCATCGTCTACACGTCCGGATCCACCAGTGCACCGAA
GGGCGTCGTACACACCCACGCCGCGTTGATCGAGCACCAGCGCAACCTCAACGCCATCCGCGGACTGGGCGCCGACGACC
GGTTGTTCTGCAACTCGCCCTTCTGCTGGATCGGGGGGATCGCGTTCTCGGTGCTGGCCACGCTGGTCGCCGGCGCGACA
CTGGTGTGCTCGAACGCCGAGGACCCGGCCGCGACCCTCGACCTGCTGGAGGCCACCCGGCCCACCATCACCAACGGCTA
CGTCGCCGGGATCGCCGCCCTGGCAGCACATCCCAGCCTGCCGCACCGTGACCTGTCGTCGATGCGACGCGGCAACCTCT
ACCCCATCATGGCGCCCGACGCCCGTCCCGCCGACCCGGACCTGCGCCACCACATGCTGGGTATGACCGAGACGGGCAGC
GTCGTTCTGCTGCACCCCGACGTCTCCGATCAGCCGGAGCACCGCCGCGGATCGTTCGGACTGCCCGCGCCCGGATTCGA
CACCAGGATCGTGGACCCGGACACCGGCGCACCGGTCGCCGCCGGCGAGGTCGGCGAGCTGTGCGTGCGTGGACCGTATG
TGATGCAGCGGTACTACAAGCGCAGCCGTGAGGAGTGCTTCGATCCCGACGGCTGGTTCCACACCGGCGATCTGGTGCGC
ACCGACGACGACGGGCTGTACTACTTCCTCGGCCGGCGCGGCGCGATGATCAAGACCGCGGGCGCCAACGTGACACCCGC
GGAGGTGGAGCGCGCGATCACCCGGGCCACGGGGGCACAGGCGTACGTCGTCGGCCTTCCCGACCGCGACCGCGGACAGG
TGGTCGCCGCGGCGGTGATCGTCGACGACCCCGGTGATTTCGACGCCGCCGCGCTGCGCGAGGCCCTGCGCGAGGAGTTG
TCGGCGTACAAGATCCCCCGCCGCATCACCGCGCTCACCCGCGACGACCTGCCCGTGATGGCGAGCGGCAAGGTCGACCT
CCCCCGGCTCACGAAGGTGTTCGATGTCCAACGCTGA

Upstream 100 bases:

>100_bases
AGCGAGATCGCGGTCGTCGACACGATCCCGCGGACCCCCTCGGGCAAGGCCGATCTGGGCGCGGTCCGGCGGTACTTCTC
GCCGGCGGTCACCGTCGACC

Downstream 100 bases:

>100_bases
CACCACGATCGACCGGTTGCTGCGCCGCAACACCGCCGCCCATCCGGAGAAGGCCGCCGTCATCGACCCGGCGTCCCGGG
TGTCCCACGGCGACCTCGAC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MAEPHTVAAVLGRWAVDRPAHPLLICDADRLDYGQAERRSARLARGLLALGAGKGTHVGLLYPNGAEFLVGMLAAARIGA
VVVPFSTFATARELAEQLRHSDTEILLSAGSFRSHDYASRLREVLGGDIPAGERLYCAAVPHLRHVFVDRHPDGGVDPAL
LTAVEDDVDGSDPLTIVYTSGSTSAPKGVVHTHAALIEHQRNLNAIRGLGADDRLFCNSPFCWIGGIAFSVLATLVAGAT
LVCSNAEDPAATLDLLEATRPTITNGYVAGIAALAAHPSLPHRDLSSMRRGNLYPIMAPDARPADPDLRHHMLGMTETGS
VVLLHPDVSDQPEHRRGSFGLPAPGFDTRIVDPDTGAPVAAGEVGELCVRGPYVMQRYYKRSREECFDPDGWFHTGDLVR
TDDDGLYYFLGRRGAMIKTAGANVTPAEVERAITRATGAQAYVVGLPDRDRGQVVAAAVIVDDPGDFDAAALREALREEL
SAYKIPRRITALTRDDLPVMASGKVDLPRLTKVFDVQR

Sequences:

>Translated_518_residues
MAEPHTVAAVLGRWAVDRPAHPLLICDADRLDYGQAERRSARLARGLLALGAGKGTHVGLLYPNGAEFLVGMLAAARIGA
VVVPFSTFATARELAEQLRHSDTEILLSAGSFRSHDYASRLREVLGGDIPAGERLYCAAVPHLRHVFVDRHPDGGVDPAL
LTAVEDDVDGSDPLTIVYTSGSTSAPKGVVHTHAALIEHQRNLNAIRGLGADDRLFCNSPFCWIGGIAFSVLATLVAGAT
LVCSNAEDPAATLDLLEATRPTITNGYVAGIAALAAHPSLPHRDLSSMRRGNLYPIMAPDARPADPDLRHHMLGMTETGS
VVLLHPDVSDQPEHRRGSFGLPAPGFDTRIVDPDTGAPVAAGEVGELCVRGPYVMQRYYKRSREECFDPDGWFHTGDLVR
TDDDGLYYFLGRRGAMIKTAGANVTPAEVERAITRATGAQAYVVGLPDRDRGQVVAAAVIVDDPGDFDAAALREALREEL
SAYKIPRRITALTRDDLPVMASGKVDLPRLTKVFDVQR
>Mature_517_residues
AEPHTVAAVLGRWAVDRPAHPLLICDADRLDYGQAERRSARLARGLLALGAGKGTHVGLLYPNGAEFLVGMLAAARIGAV
VVPFSTFATARELAEQLRHSDTEILLSAGSFRSHDYASRLREVLGGDIPAGERLYCAAVPHLRHVFVDRHPDGGVDPALL
TAVEDDVDGSDPLTIVYTSGSTSAPKGVVHTHAALIEHQRNLNAIRGLGADDRLFCNSPFCWIGGIAFSVLATLVAGATL
VCSNAEDPAATLDLLEATRPTITNGYVAGIAALAAHPSLPHRDLSSMRRGNLYPIMAPDARPADPDLRHHMLGMTETGSV
VLLHPDVSDQPEHRRGSFGLPAPGFDTRIVDPDTGAPVAAGEVGELCVRGPYVMQRYYKRSREECFDPDGWFHTGDLVRT
DDDGLYYFLGRRGAMIKTAGANVTPAEVERAITRATGAQAYVVGLPDRDRGQVVAAAVIVDDPGDFDAAALREALREELS
AYKIPRRITALTRDDLPVMASGKVDLPRLTKVFDVQR

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=521, Percent_Identity=25.3358925143954, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI187761345, Length=538, Percent_Identity=26.2081784386617, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI187761343, Length=538, Percent_Identity=26.2081784386617, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI115511026, Length=488, Percent_Identity=22.9508196721311, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38505220, Length=514, Percent_Identity=24.3190661478599, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI42544132, Length=531, Percent_Identity=22.9755178907721, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI28416953, Length=566, Percent_Identity=27.0318021201413, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI122937307, Length=543, Percent_Identity=23.2044198895028, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157311624, Length=513, Percent_Identity=22.6120857699805, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157311622, Length=513, Percent_Identity=22.6120857699805, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI58082049, Length=514, Percent_Identity=22.7626459143969, Blast_Score=83, Evalue=7e-16,
Organism=Escherichia coli, GI1788107, Length=517, Percent_Identity=26.8858800773694, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI221142682, Length=503, Percent_Identity=26.2425447316103, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1790505, Length=524, Percent_Identity=25.381679389313, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693145, Length=509, Percent_Identity=25.9332023575639, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1786810, Length=350, Percent_Identity=27.1428571428571, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786529, Length=555, Percent_Identity=25.7657657657658, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=532, Percent_Identity=26.1278195488722, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=532, Percent_Identity=26.1278195488722, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=532, Percent_Identity=26.1278195488722, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17560308, Length=523, Percent_Identity=25.8126195028681, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=510, Percent_Identity=25.2941176470588, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=516, Percent_Identity=25.1937984496124, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=492, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=509, Percent_Identity=25.3438113948919, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=516, Percent_Identity=24.8062015503876, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=177, Percent_Identity=32.2033898305085, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=392, Percent_Identity=25, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=513, Percent_Identity=23.3918128654971, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323182, Length=563, Percent_Identity=26.6429840142096, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6319264, Length=557, Percent_Identity=25.673249551167, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319699, Length=485, Percent_Identity=25.1546391752577, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24581924, Length=526, Percent_Identity=25.8555133079848, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355181, Length=471, Percent_Identity=26.7515923566879, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21356441, Length=330, Percent_Identity=28.1818181818182, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI18859661, Length=535, Percent_Identity=25.4205607476636, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648253, Length=368, Percent_Identity=25, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI62472339, Length=535, Percent_Identity=25.4205607476636, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24648255, Length=368, Percent_Identity=25.2717391304348, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24667955, Length=535, Percent_Identity=25.4205607476636, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24653035, Length=365, Percent_Identity=24.3835616438356, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI21356947, Length=346, Percent_Identity=24.8554913294798, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24667959, Length=503, Percent_Identity=25.2485089463221, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI161076582, Length=380, Percent_Identity=22.8947368421053, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21358303, Length=363, Percent_Identity=25.8953168044077, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281365686, Length=362, Percent_Identity=24.3093922651934, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17933690, Length=522, Percent_Identity=22.4137931034483, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648257, Length=320, Percent_Identity=25, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 55612; Mature: 55480

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPHTVAAVLGRWAVDRPAHPLLICDADRLDYGQAERRSARLARGLLALGAGKGTHVGL
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
LYPNGAEFLVGMLAAARIGAVVVPFSTFATARELAEQLRHSDTEILLSAGSFRSHDYASR
ECCCHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
LREVLGGDIPAGERLYCAAVPHLRHVFVDRHPDGGVDPALLTAVEDDVDGSDPLTIVYTS
HHHHHCCCCCCCCEEEEEECHHHHHHHEECCCCCCCCHHHHEEECCCCCCCCCEEEEEEC
GSTSAPKGVVHTHAALIEHQRNLNAIRGLGADDRLFCNSPFCWIGGIAFSVLATLVAGAT
CCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCE
LVCSNAEDPAATLDLLEATRPTITNGYVAGIAALAAHPSLPHRDLSSMRRGNLYPIMAPD
EEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCC
ARPADPDLRHHMLGMTETGSVVLLHPDVSDQPEHRRGSFGLPAPGFDTRIVDPDTGAPVA
CCCCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
AGEVGELCVRGPYVMQRYYKRSREECFDPDGWFHTGDLVRTDDDGLYYFLGRRGAMIKTA
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEECCCEEEECCCCEEEEECCCCCEEEEC
GANVTPAEVERAITRATGAQAYVVGLPDRDRGQVVAAAVIVDDPGDFDAAALREALREEL
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
SAYKIPRRITALTRDDLPVMASGKVDLPRLTKVFDVQR
HHHCCCHHHHHHCCCCCCEEECCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AEPHTVAAVLGRWAVDRPAHPLLICDADRLDYGQAERRSARLARGLLALGAGKGTHVGL
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
LYPNGAEFLVGMLAAARIGAVVVPFSTFATARELAEQLRHSDTEILLSAGSFRSHDYASR
ECCCHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
LREVLGGDIPAGERLYCAAVPHLRHVFVDRHPDGGVDPALLTAVEDDVDGSDPLTIVYTS
HHHHHCCCCCCCCEEEEEECHHHHHHHEECCCCCCCCHHHHEEECCCCCCCCCEEEEEEC
GSTSAPKGVVHTHAALIEHQRNLNAIRGLGADDRLFCNSPFCWIGGIAFSVLATLVAGAT
CCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCE
LVCSNAEDPAATLDLLEATRPTITNGYVAGIAALAAHPSLPHRDLSSMRRGNLYPIMAPD
EEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCC
ARPADPDLRHHMLGMTETGSVVLLHPDVSDQPEHRRGSFGLPAPGFDTRIVDPDTGAPVA
CCCCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
AGEVGELCVRGPYVMQRYYKRSREECFDPDGWFHTGDLVRTDDDGLYYFLGRRGAMIKTA
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEECCCEEEECCCCEEEEECCCCCEEEEC
GANVTPAEVERAITRATGAQAYVVGLPDRDRGQVVAAAVIVDDPGDFDAAALREALREEL
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
SAYKIPRRITALTRDDLPVMASGKVDLPRLTKVFDVQR
HHHCCCHHHHHHCCCCCCEEECCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]