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The map label for this gene is nuoF [H]

Identifier: 119867645

GI number: 119867645

Start: 1717584

End: 1718900

Strand: Reverse

Name: nuoF [H]

Synonym: Mkms_1599

Alternate gene names: 119867645

Gene position: 1718900-1717584 (Counterclockwise)

Preceding gene: 119867646

Following gene: 119867644

Centisome position: 29.96

GC content: 68.64

Gene sequence:

>1317_bases
ATGACGGCGCTCACCCCGGTGCTGAGCCGGTTCTGGGACGAACCCGAATCGTGGACGCTGGAGACCTATCGGCGCCACGG
CGGATACCGGGCGCTCGAACAGGCGCTCGGGACGGCGCCCGACGACGTGATCACCACCATCAAGGATTCGGGACTGCGTG
GCCGGGGCGGTGCGGGCTTCCCCACCGGCACCAAATGGTCGTTCATCCCGCAGGGTGACGAGGGGCCCGCGGCCAAACCG
CACTACCTGGTGATCAACGCCGATGAGTCGGAACCCGGTACGTGCAAGGACATTCCGCTGCTGCTGACCACGCCGCACTT
CCTCGTGGAGGGCGCGATCATCGCCGCGTACGCGATCCGCGCCCACCACGCGTTCATCTACGTGCGCGGTGAGGTGGTGC
CGGTGCTGCGCCGGCTGCAGGCCGCCGTCGCCGAGGCCTACGAGGCCGGCTACCTGGGTACCGACATCCGCGGATCCGGT
TTCGACCTCGACCTGATCGTGCACGCCGGGGCCGGGGCCTACATCTGCGGTGAGGAGACGGCACTGCTCGACTCCCTGGA
AGGCCGCCGCGGCCAGCCCCGGCTGCGCCCGCCCTTCCCCGCCGTCGCCGGGCTCTACGCGTGCCCGACGGTGGTCAACA
ACGTCGAATCCATCGCCAGCGTGCCGCCGATCCTGCTGGGCGGCATCGACTGGTTCCGTTCGATGGGGTCGGAGAAATCG
CCGGGCTTCACGCTGTATTCGCTGTCCGGGCACGTCACCCGGCCCGGACAGTACGAGGCGCCCCTGGGCATCACGCTGCG
CGAACTGCTGGAGTACGCCGGCGGCGTCCGGGCCGGCCACGAGCTGAAGTTCTGGACGCCGGGCGGGTCGTCGACGCCGC
TGCTCACCGGCGAACACCTCGATGTGCCACTGGATTACGAGGGGATGGCCTCGGTCGGCTCGATGCTCGGCACCAAGGCG
CTGCAGATCTTCGACGAGACCACCTGCGTGGTGCGCGCCGTCCGCCGCTGGACGCAGTTCTACGCCCACGAGTCCTGCGG
CAAGTGCACACCGTGCCGCGAAGGCACCTACTGGCTCGCCCAGATCTACGCCCGCCTCGAGAGCGGCGAGGCCACCGAGG
CCGACGTCGACAAACTCCTCGACATCTCCGACACCATCCTCGGGAAGTCGTTCTGCGCGTTGGGTGACGGTGCGGCCAGC
CCGATCATCTCGTCGATCAAGTACTTCCGCGACGAGTACCTGGCCCATCTCGGTAACGGCTGCCCGTTCGATCCGCACGC
ATCGACGCTGATGGCCGGACAGGAGGTGGGGGTGTGA

Upstream 100 bases:

>100_bases
ATCCGGAGATCGTCGAGGCGGCCGACTCGACCAGGGACGAACCCGCCCCCGGACCCTCGGCCGCGGTACCGCGTCCCGGC
GACGATTCGGAGCCGGGGTC

Downstream 100 bases:

>100_bases
CGTCCCGTACGCCACCGCGCTCGCCGAATGTGCGGTTCCATCCGCGACACACCGCCCTATGCGTACGAAACCGCACACTC
GCGGCGGACGAAAGTGGGGG

Product: NADH-quinone oxidoreductase, F subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTALTPVLSRFWDEPESWTLETYRRHGGYRALEQALGTAPDDVITTIKDSGLRGRGGAGFPTGTKWSFIPQGDEGPAAKP
HYLVINADESEPGTCKDIPLLLTTPHFLVEGAIIAAYAIRAHHAFIYVRGEVVPVLRRLQAAVAEAYEAGYLGTDIRGSG
FDLDLIVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYACPTVVNNVESIASVPPILLGGIDWFRSMGSEKS
PGFTLYSLSGHVTRPGQYEAPLGITLRELLEYAGGVRAGHELKFWTPGGSSTPLLTGEHLDVPLDYEGMASVGSMLGTKA
LQIFDETTCVVRAVRRWTQFYAHESCGKCTPCREGTYWLAQIYARLESGEATEADVDKLLDISDTILGKSFCALGDGAAS
PIISSIKYFRDEYLAHLGNGCPFDPHASTLMAGQEVGV

Sequences:

>Translated_438_residues
MTALTPVLSRFWDEPESWTLETYRRHGGYRALEQALGTAPDDVITTIKDSGLRGRGGAGFPTGTKWSFIPQGDEGPAAKP
HYLVINADESEPGTCKDIPLLLTTPHFLVEGAIIAAYAIRAHHAFIYVRGEVVPVLRRLQAAVAEAYEAGYLGTDIRGSG
FDLDLIVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYACPTVVNNVESIASVPPILLGGIDWFRSMGSEKS
PGFTLYSLSGHVTRPGQYEAPLGITLRELLEYAGGVRAGHELKFWTPGGSSTPLLTGEHLDVPLDYEGMASVGSMLGTKA
LQIFDETTCVVRAVRRWTQFYAHESCGKCTPCREGTYWLAQIYARLESGEATEADVDKLLDISDTILGKSFCALGDGAAS
PIISSIKYFRDEYLAHLGNGCPFDPHASTLMAGQEVGV
>Mature_437_residues
TALTPVLSRFWDEPESWTLETYRRHGGYRALEQALGTAPDDVITTIKDSGLRGRGGAGFPTGTKWSFIPQGDEGPAAKPH
YLVINADESEPGTCKDIPLLLTTPHFLVEGAIIAAYAIRAHHAFIYVRGEVVPVLRRLQAAVAEAYEAGYLGTDIRGSGF
DLDLIVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYACPTVVNNVESIASVPPILLGGIDWFRSMGSEKSP
GFTLYSLSGHVTRPGQYEAPLGITLRELLEYAGGVRAGHELKFWTPGGSSTPLLTGEHLDVPLDYEGMASVGSMLGTKAL
QIFDETTCVVRAVRRWTQFYAHESCGKCTPCREGTYWLAQIYARLESGEATEADVDKLLDISDTILGKSFCALGDGAASP
IISSIKYFRDEYLAHLGNGCPFDPHASTLMAGQEVGV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=398, Percent_Identity=47.4874371859297, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI20149568, Length=398, Percent_Identity=47.4874371859297, Blast_Score=372, Evalue=1e-103,
Organism=Escherichia coli, GI1788620, Length=413, Percent_Identity=46.9733656174334, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17531727, Length=390, Percent_Identity=45.8974358974359, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI19920794, Length=398, Percent_Identity=47.4874371859297, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45550452, Length=396, Percent_Identity=46.969696969697, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24653819, Length=396, Percent_Identity=36.3636363636364, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24653817, Length=349, Percent_Identity=36.1031518624642, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47196; Mature: 47065

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALTPVLSRFWDEPESWTLETYRRHGGYRALEQALGTAPDDVITTIKDSGLRGRGGAGF
CCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
PTGTKWSFIPQGDEGPAAKPHYLVINADESEPGTCKDIPLLLTTPHFLVEGAIIAAYAIR
CCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
AHHAFIYVRGEVVPVLRRLQAAVAEAYEAGYLGTDIRGSGFDLDLIVHAGAGAYICGEET
HEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCHH
ALLDSLEGRRGQPRLRPPFPAVAGLYACPTVVNNVESIASVPPILLGGIDWFRSMGSEKS
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
PGFTLYSLSGHVTRPGQYEAPLGITLRELLEYAGGVRAGHELKFWTPGGSSTPLLTGEHL
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEECCCCC
DVPLDYEGMASVGSMLGTKALQIFDETTCVVRAVRRWTQFYAHESCGKCTPCREGTYWLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
QIYARLESGEATEADVDKLLDISDTILGKSFCALGDGAASPIISSIKYFRDEYLAHLGNG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
CPFDPHASTLMAGQEVGV
CCCCCCHHHHHCCHHCCC
>Mature Secondary Structure 
TALTPVLSRFWDEPESWTLETYRRHGGYRALEQALGTAPDDVITTIKDSGLRGRGGAGF
CCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
PTGTKWSFIPQGDEGPAAKPHYLVINADESEPGTCKDIPLLLTTPHFLVEGAIIAAYAIR
CCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
AHHAFIYVRGEVVPVLRRLQAAVAEAYEAGYLGTDIRGSGFDLDLIVHAGAGAYICGEET
HEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCHH
ALLDSLEGRRGQPRLRPPFPAVAGLYACPTVVNNVESIASVPPILLGGIDWFRSMGSEKS
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
PGFTLYSLSGHVTRPGQYEAPLGITLRELLEYAGGVRAGHELKFWTPGGSSTPLLTGEHL
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEECCCCC
DVPLDYEGMASVGSMLGTKALQIFDETTCVVRAVRRWTQFYAHESCGKCTPCREGTYWLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
QIYARLESGEATEADVDKLLDISDTILGKSFCALGDGAASPIISSIKYFRDEYLAHLGNG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
CPFDPHASTLMAGQEVGV
CCCCCCHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]