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The map label for this gene is dsbB

Identifier: 119899661

GI number: 119899661

Start: 3706379

End: 3706879

Strand: Direct

Name: dsbB

Synonym: azo3372

Alternate gene names: 119899661

Gene position: 3706379-3706879 (Clockwise)

Preceding gene: 119899660

Following gene: 119899669

Centisome position: 84.7

GC content: 69.66

Gene sequence:

>501_bases
ATGATCGCCCTCCCCCGCAACCGCCGTCCGCTCTTTCTCGCCGTGTTCGCCTACTGCGCGGCGCTGCTCGCCTTCGGGCT
CTACCTGCAGCACTACCAGGGCATCGAGCCGTGCCCGATGTGCATCATGCAGCGCTATGCATTCGCACTGGTGGGCGTGA
TCGCGCTGGTCGCGGGCCTGCACGGACCGCGCGGCGCGGGCGTGCGCGTGTATGGGGGACTGCTGTTGCTCACGGCGCTG
GCCGGCGGCAGCGTCGCCGCGCGCCAGACGTGGATGCAGCTCTACCCGCCGGAAATTCCGGAGTGCGGGCCAGGGCTGGA
ATACATGCTGGAGAGCTTTCCGCTGACTTCGGCGCTGCCGATGATCTTCCGCGGCGCGGGCGACTGCAGCGCGATCGACT
GGACCTTCCTCGGCCTGTCGCTCGCCAACTGGTCGCTGCTCAACTTCGGCGCCGCGGCGCTGCTCGCGCTGTGGCTGCTG
TTCGGCCGCCGCGTCCGCTGA

Upstream 100 bases:

>100_bases
GGATGGCGGAACTTTCACCGCGCCACGGCGACGAAGCCCGGCCCGCGCGCCTCACCTACAATCGCGTCCGCACCCCGAAC
AACCACGCCCGCTCTCACCC

Downstream 100 bases:

>100_bases
CGCGGGTTCAGCCGACCACGCGCACGATCGCGCCGCCGATCTCGTAGCGGCCGTCGCCGAGCGCGTGACAGCGCTTGACC
TGCAGTTTGGTCACCAGCGG

Product: putative disulfide bond formation protein B

Products: glutathione disulfide; Protein dithiol

Alternate protein names: Disulfide oxidoreductase

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MIALPRNRRPLFLAVFAYCAALLAFGLYLQHYQGIEPCPMCIMQRYAFALVGVIALVAGLHGPRGAGVRVYGGLLLLTAL
AGGSVAARQTWMQLYPPEIPECGPGLEYMLESFPLTSALPMIFRGAGDCSAIDWTFLGLSLANWSLLNFGAAALLALWLL
FGRRVR

Sequences:

>Translated_166_residues
MIALPRNRRPLFLAVFAYCAALLAFGLYLQHYQGIEPCPMCIMQRYAFALVGVIALVAGLHGPRGAGVRVYGGLLLLTAL
AGGSVAARQTWMQLYPPEIPECGPGLEYMLESFPLTSALPMIFRGAGDCSAIDWTFLGLSLANWSLLNFGAAALLALWLL
FGRRVR
>Mature_166_residues
MIALPRNRRPLFLAVFAYCAALLAFGLYLQHYQGIEPCPMCIMQRYAFALVGVIALVAGLHGPRGAGVRVYGGLLLLTAL
AGGSVAARQTWMQLYPPEIPECGPGLEYMLESFPLTSALPMIFRGAGDCSAIDWTFLGLSLANWSLLNFGAAALLALWLL
FGRRVR

Specific function: Required for disulfide bond formation in some periplasmic proteins. Acts by oxidizing the DsbA protein

COG id: COG1495

COG function: function code O; Disulfide bond formation protein DsbB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DsbB family

Homologues:

Organism=Escherichia coli, GI1787433, Length=133, Percent_Identity=32.3308270676692, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DSBB_AZOSB (A1KAY2)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934874.1
- ProteinModelPortal:   A1KAY2
- SMR:   A1KAY2
- STRING:   A1KAY2
- GeneID:   4609183
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3372
- eggNOG:   COG1495
- HOGENOM:   HBG637909
- OMA:   WAWARAK
- ProtClustDB:   CLSK766721
- BioCyc:   ASP62928:AZO3372-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00286
- InterPro:   IPR003752
- InterPro:   IPR022920

Pfam domain/function: PF02600 DsbB

EC number: 1.8.4.2

Molecular weight: Translated: 18021; Mature: 18021

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1de6c72c)-; HASH(0x1eb52a68)-; HASH(0x1ee90ec4)-; HASH(0x1db41ddc)-;

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALPRNRRPLFLAVFAYCAALLAFGLYLQHYQGIEPCPMCIMQRYAFALVGVIALVAGL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
HGPRGAGVRVYGGLLLLTALAGGSVAARQTWMQLYPPEIPECGPGLEYMLESFPLTSALP
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHH
MIFRGAGDCSAIDWTFLGLSLANWSLLNFGAAALLALWLLFGRRVR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure
MIALPRNRRPLFLAVFAYCAALLAFGLYLQHYQGIEPCPMCIMQRYAFALVGVIALVAGL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
HGPRGAGVRVYGGLLLLTALAGGSVAARQTWMQLYPPEIPECGPGLEYMLESFPLTSALP
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHH
MIFRGAGDCSAIDWTFLGLSLANWSLLNFGAAALLALWLLFGRRVR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glutathione; Protein disulfide

Specific reaction: 2 glutathione + protein-disulfide = glutathione disulfide + protein-dithiol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA