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The map label for this gene is dosC [H]

Identifier: 119899139

GI number: 119899139

Start: 3129466

End: 3131328

Strand: Reverse

Name: dosC [H]

Synonym: azo2849

Alternate gene names: 119899139

Gene position: 3131328-3129466 (Counterclockwise)

Preceding gene: 119899140

Following gene: 119899135

Centisome position: 71.56

GC content: 67.04

Gene sequence:

>1863_bases
ATGAACTTGAATCGGAAGATAACGGCGCTGTTTTCGGCCGTGGCGGTGGGCATCCTGGTGATGCTGGTGGCGATCAGCCT
GTACGCCTTCCGCAGTTATTCCATCGCCTCCTCGACCGCGCATGTGCGCACCGCCGCCGAGATGGTGCGGGTGCATCTGA
CCGAGGCGATGATCCACGGGGTCATCGACAAGCGCGAGCAGTTTCTGGACCGCCTGATGGAGGTCCAGGGCCTGCGCGCG
GCGCGGGTGCTGCGGTCCACCCATGTCAACGAACAGTTCGGTGCCGGCATGGCGCGCGAGATGGAGCCTGACGAAGTCGA
GAAGCAGGTGCTGGCCGACGGTCACGCGCGCTATCTGCTGGAAAACGAACTGGGCGATGCGGTGTTCCGCGGCACGATTC
CGTTTGCCGCCTCCGCGCGCGGCCGCCCCAACTGCCTGCAGTGCCACCGCGTCAGCGAGGGCACCGTGCTGGGCGCGGTG
ACGATCGAGCTGTCGCTGCAGGAGCTCAAGCGCAGCGCGCTGTTTACCGTGGCGGTCATCATCGTCACCGTCGCGCTGTT
CTCGCTGCTGGCGATCTTTGCCGTGCGACGCCTGATCATGCCGGTGGGTGACACCGCGCAGGCGGTGGAGCGGGCGGTGC
AGCGTGCGCTGCGCGGCGACTTCAAGGGCCGGGTGGAAAAGCGCACCGACGACGAAATCGGCAAGATCGCCACCGAAACC
AACAGCCTGCTCGGCTTCCTCGACGAAGGCCTGTCGCGCATTACCCAGCGCGTCACCCAGCTCACCGGGCGTACCCCGCA
GCACGACGAGAACCAGCTCGAAGCCACCATCGACATGGTCAACAGCCTGGCCGACGCGGCCGGCTTCAAGCAGGCGATCG
AGGAGGACGAGAGCAAGACCGAGATCTACGACCGCTTCAGCGGCGTGCTGCTGGAGCGCTTCGACGTGCGCGAGTTCTCC
ATCTACGAGGCCGAGGGCAGCAAGCAACTGCAGCCGATCGCGGTGGATGGTGTGCAGGGCGCGCCTTGCCGCTGGTGCGA
TCCGCAGATTCTGGTGCGCAGCGACATGTGCCGCGCGCGCCGCACCGGCCATGCGGTCAATGGCCTGATCGAGCCGGGCA
TCTGCTACGCCTTCCATCCCCCCGCCGAGGGCGGGGCGCGGCGCCACTACTGCGTGCCCATCATCCAGTCGGGCACCGTC
GGCAGTGTCATCCAGCTGGTGGTGGCGGAGGAGCGCGCGCCGGCGCTGGTCGAGCAGGCGCCCTACATCCACGTCTATCT
GCGCGAGATGGCGCCGGTGCTCGAAGCCAAGCGCCTCACCGAAACCCTGCGCGACTCCTCGCTGCGCGACGCAATGACCG
GGCTCAACAACCGGCGCTTCCTCGAGGAGTACATGGACACGCTGGTGGCCAGCGCGCGGCGGCGGCGGATTCCGCTGGCG
GTGCTGGTGCTGGACCTCGACTACTTCAAGGTGGTGAACGACAGCTACGGCCACGACGCGGGCGACGCGGTGCTGAAGGC
GCTGGCCGGGGTGCTCAAGCGCACGGTGCGCGCGTCCGACATGGTGATCCGCTTCGGCGGCGAGGAATTCCTGATCGTGC
TGCAGGAGGCCGATGCCGAGGCGGCGCTGAAGGTGGCCGAGAACATCCGCTCCGCCGTGGAACAAATGCGGGTGCAGGTG
GCCGGCGCGGTGCTGCAGAAGACGATCTCGATCGGGCTGTCGATGTTCCCCGACGACAGCGACACCTTCTGGCAGGCGAT
CAAGTTCGCCGACGTGGCGCTTTACCGCGCCAAGGAAAACGGTCGCAACCAGGTGGTGCGGTTCACGCCCGACATGTGGA
GCGGGCAGGGCGAGTCGTACTGA

Upstream 100 bases:

>100_bases
CGCTTTGGGCGTGATTTCCGTCACGCTGCCCGCGGTAAACACGAGTAAGCCGAAAGTACTAAAAGAAAGATAATCGGCGC
TTGCGGGCAGAGGTGGCTTC

Downstream 100 bases:

>100_bases
AGCGGAAGACCGCCTCAGCGGCAGTGGGTGTTCATGTACTGGTGCTGGAAGCGCGCCTTGCCCTCGCGCAGGAAGCGGTC
GAAATCCCACTCGCCCGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MNLNRKITALFSAVAVGILVMLVAISLYAFRSYSIASSTAHVRTAAEMVRVHLTEAMIHGVIDKREQFLDRLMEVQGLRA
ARVLRSTHVNEQFGAGMAREMEPDEVEKQVLADGHARYLLENELGDAVFRGTIPFAASARGRPNCLQCHRVSEGTVLGAV
TIELSLQELKRSALFTVAVIIVTVALFSLLAIFAVRRLIMPVGDTAQAVERAVQRALRGDFKGRVEKRTDDEIGKIATET
NSLLGFLDEGLSRITQRVTQLTGRTPQHDENQLEATIDMVNSLADAAGFKQAIEEDESKTEIYDRFSGVLLERFDVREFS
IYEAEGSKQLQPIAVDGVQGAPCRWCDPQILVRSDMCRARRTGHAVNGLIEPGICYAFHPPAEGGARRHYCVPIIQSGTV
GSVIQLVVAEERAPALVEQAPYIHVYLREMAPVLEAKRLTETLRDSSLRDAMTGLNNRRFLEEYMDTLVASARRRRIPLA
VLVLDLDYFKVVNDSYGHDAGDAVLKALAGVLKRTVRASDMVIRFGGEEFLIVLQEADAEAALKVAENIRSAVEQMRVQV
AGAVLQKTISIGLSMFPDDSDTFWQAIKFADVALYRAKENGRNQVVRFTPDMWSGQGESY

Sequences:

>Translated_620_residues
MNLNRKITALFSAVAVGILVMLVAISLYAFRSYSIASSTAHVRTAAEMVRVHLTEAMIHGVIDKREQFLDRLMEVQGLRA
ARVLRSTHVNEQFGAGMAREMEPDEVEKQVLADGHARYLLENELGDAVFRGTIPFAASARGRPNCLQCHRVSEGTVLGAV
TIELSLQELKRSALFTVAVIIVTVALFSLLAIFAVRRLIMPVGDTAQAVERAVQRALRGDFKGRVEKRTDDEIGKIATET
NSLLGFLDEGLSRITQRVTQLTGRTPQHDENQLEATIDMVNSLADAAGFKQAIEEDESKTEIYDRFSGVLLERFDVREFS
IYEAEGSKQLQPIAVDGVQGAPCRWCDPQILVRSDMCRARRTGHAVNGLIEPGICYAFHPPAEGGARRHYCVPIIQSGTV
GSVIQLVVAEERAPALVEQAPYIHVYLREMAPVLEAKRLTETLRDSSLRDAMTGLNNRRFLEEYMDTLVASARRRRIPLA
VLVLDLDYFKVVNDSYGHDAGDAVLKALAGVLKRTVRASDMVIRFGGEEFLIVLQEADAEAALKVAENIRSAVEQMRVQV
AGAVLQKTISIGLSMFPDDSDTFWQAIKFADVALYRAKENGRNQVVRFTPDMWSGQGESY
>Mature_620_residues
MNLNRKITALFSAVAVGILVMLVAISLYAFRSYSIASSTAHVRTAAEMVRVHLTEAMIHGVIDKREQFLDRLMEVQGLRA
ARVLRSTHVNEQFGAGMAREMEPDEVEKQVLADGHARYLLENELGDAVFRGTIPFAASARGRPNCLQCHRVSEGTVLGAV
TIELSLQELKRSALFTVAVIIVTVALFSLLAIFAVRRLIMPVGDTAQAVERAVQRALRGDFKGRVEKRTDDEIGKIATET
NSLLGFLDEGLSRITQRVTQLTGRTPQHDENQLEATIDMVNSLADAAGFKQAIEEDESKTEIYDRFSGVLLERFDVREFS
IYEAEGSKQLQPIAVDGVQGAPCRWCDPQILVRSDMCRARRTGHAVNGLIEPGICYAFHPPAEGGARRHYCVPIIQSGTV
GSVIQLVVAEERAPALVEQAPYIHVYLREMAPVLEAKRLTETLRDSSLRDAMTGLNNRRFLEEYMDTLVASARRRRIPLA
VLVLDLDYFKVVNDSYGHDAGDAVLKALAGVLKRTVRASDMVIRFGGEEFLIVLQEADAEAALKVAENIRSAVEQMRVQV
AGAVLQKTISIGLSMFPDDSDTFWQAIKFADVALYRAKENGRNQVVRFTPDMWSGQGESY

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=41.9161676646707, Blast_Score=129, Evalue=8e-31,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=36.8715083798883, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=40.3409090909091, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=35.1851851851852, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788381, Length=186, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=36.6863905325444, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=36.0759493670886, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=31.1111111111111, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=34.5911949685535, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081974, Length=168, Percent_Identity=31.547619047619, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081977, Length=200, Percent_Identity=29.5, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=29.0909090909091, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=204, Percent_Identity=29.9019607843137, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 68668; Mature: 68668

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNRKITALFSAVAVGILVMLVAISLYAFRSYSIASSTAHVRTAAEMVRVHLTEAMIHG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VIDKREQFLDRLMEVQGLRAARVLRSTHVNEQFGAGMAREMEPDEVEKQVLADGHARYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHCCHHHHHH
ENELGDAVFRGTIPFAASARGRPNCLQCHRVSEGTVLGAVTIELSLQELKRSALFTVAVI
HHHHCCHHHCCCCCCCCCCCCCCCCHHHEECCCCCEEEEEEEEHHHHHHHHHHHHHHHHH
IVTVALFSLLAIFAVRRLIMPVGDTAQAVERAVQRALRGDFKGRVEKRTDDEIGKIATET
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
NSLLGFLDEGLSRITQRVTQLTGRTPQHDENQLEATIDMVNSLADAAGFKQAIEEDESKT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EIYDRFSGVLLERFDVREFSIYEAEGSKQLQPIAVDGVQGAPCRWCDPQILVRSDMCRAR
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHH
RTGHAVNGLIEPGICYAFHPPAEGGARRHYCVPIIQSGTVGSVIQLVVAEERAPALVEQA
HCCCHHCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHC
PYIHVYLREMAPVLEAKRLTETLRDSSLRDAMTGLNNRRFLEEYMDTLVASARRRRIPLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEE
VLVLDLDYFKVVNDSYGHDAGDAVLKALAGVLKRTVRASDMVIRFGGEEFLIVLQEADAE
EEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCHH
AALKVAENIRSAVEQMRVQVAGAVLQKTISIGLSMFPDDSDTFWQAIKFADVALYRAKEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHCCC
GRNQVVRFTPDMWSGQGESY
CCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNLNRKITALFSAVAVGILVMLVAISLYAFRSYSIASSTAHVRTAAEMVRVHLTEAMIHG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VIDKREQFLDRLMEVQGLRAARVLRSTHVNEQFGAGMAREMEPDEVEKQVLADGHARYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHCCHHHHHH
ENELGDAVFRGTIPFAASARGRPNCLQCHRVSEGTVLGAVTIELSLQELKRSALFTVAVI
HHHHCCHHHCCCCCCCCCCCCCCCCHHHEECCCCCEEEEEEEEHHHHHHHHHHHHHHHHH
IVTVALFSLLAIFAVRRLIMPVGDTAQAVERAVQRALRGDFKGRVEKRTDDEIGKIATET
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
NSLLGFLDEGLSRITQRVTQLTGRTPQHDENQLEATIDMVNSLADAAGFKQAIEEDESKT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EIYDRFSGVLLERFDVREFSIYEAEGSKQLQPIAVDGVQGAPCRWCDPQILVRSDMCRAR
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHH
RTGHAVNGLIEPGICYAFHPPAEGGARRHYCVPIIQSGTVGSVIQLVVAEERAPALVEQA
HCCCHHCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHC
PYIHVYLREMAPVLEAKRLTETLRDSSLRDAMTGLNNRRFLEEYMDTLVASARRRRIPLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEE
VLVLDLDYFKVVNDSYGHDAGDAVLKALAGVLKRTVRASDMVIRFGGEEFLIVLQEADAE
EEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCHH
AALKVAENIRSAVEQMRVQVAGAVLQKTISIGLSMFPDDSDTFWQAIKFADVALYRAKEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHCCC
GRNQVVRFTPDMWSGQGESY
CCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]