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The map label for this gene is proP [H]

Identifier: 119899140

GI number: 119899140

Start: 3131436

End: 3133073

Strand: Reverse

Name: proP [H]

Synonym: azo2850

Alternate gene names: 119899140

Gene position: 3133073-3131436 (Counterclockwise)

Preceding gene: 119899142

Following gene: 119899139

Centisome position: 71.6

GC content: 70.82

Gene sequence:

>1638_bases
ATGACCCGTCCGATGCCTTCCCCTCCCGAATCCACCGCGTCCGCAGTGCCCGCCGACTCCGACGGTCATTCCGCCCCGGA
CATGCCTGGCGCCGGGCAGGTGCAGCGCAAGGTGGCGGTCGCGGCCTGCTTCGGCACCTTCCTCGAGTGGTACGACTTCC
TCACCTTCGCGTCGCTCGCCACCTACTTCGGCACGCTGTTCTTTCCGCCGGATGACCCGGTCACCGCGCTGCTGGCGAGC
TTGGGCACATTCGGCGTCGGCATGGTGGTGCGGCCGCTGGGGGCGGCGCTGTTCGGGTCGCTGGGCGACCGTTACGGGCG
GCGCGCCATCTTTCTCGCCACCATCGTGCTGATGGGCGGTTCCACCTTCCTGGTCGGCCTGCTGCCCACCTACGCCAGCA
TCGGGCTTTTCGCGCCCTTGCTGCTGTTGGTGCTGCGCATGCTGCAGGGGCTGTCGGTGGGCGGGGAGATCGGCGGCGCG
GCGGTGTATCTCACCGAGCACGCGCCGGCCACCCGGCGCGGCGCCTACACCAGCGTGCTGCAGTTGATGGGGCCGCTCGG
CATGCTCGCGTCCACCCTGCAGATCGTGGCGCTGCAGGCGGTGCTGAGCGAGGCGGAGTTCCGCGACTGGGGCTGGCGGG
TGCCGTTCCTGATCTCGATCGTGCTGCTGGCGGTGTCGATCAAGAGCCGGCTGAGCCTGCACGAGTCGCCGGTGTTCCGC
CGCCTGCGCGAGCAGAACGCGCTCGCAAAGACGCCGCTGCGCGAGTGCTTCCGTGATCGCCAGACGCTGGGCCGCATGGC
GCTGCTGTTCTTCTGCATCTCGTCGGGCGGCTCGCTGCTGTTCTTCTCGTCGCAGGTCTATACGGCGGTGTTCCTGAAGA
ACGTGGTCAAGCTCGACGCCGCTACCGCGAGCGGGCTGGTGATGGTCGCCACGGTCGTGCTGTTTCCCCTCACCGTGCTG
TGCGGCTGGCTGTCGGACCGCATCGGCCGCCGCCCGGTGTTGCTGGCCGGGCTGGTGCTGGGGGCGCTCACCGTGCTGCC
GGTGTTCCACGGCCTGCTCGAACACGGCAACCCCGCGCTGGCGCGCTTCAACGCCTCGGTAGCGGTGGAACTGGCCGGCG
AGCAGTGCCGCTACGACCCCTTTGCCGGCGTGCGCAACGACTGCGAGCGCTACCAGGAACTGTTCGCCCGGCTCGGCGTG
GTGCATCAGCAGCGCGAGGGCGCGCTGGCGGTGAAGGTGGGCGGGCAGACCATCGCCGGCTACCAGCCGGAGGCCGTCAC
CCGCGCGCTGGTGGAGGCGGGACGCCCGGCGCAGGCGGATCCGGCGCAGGTGAACCGCCCGGCCCTGATCGCGCTGTTGC
TGGTGCTGGTGGTGGCGCTCGCCTGCATCACCGGGCCGCAGACGGCCACGCTCGCCGAACTCTTTCCGGCCCGCACGCGC
TACTCCGCGGTGGCCTTGCCCCACAACCTCGCGGCGGGCTGGATCGGCGGCCTGTCGCCGTTCATGGTTACCTGGCTCAG
CGTGCGCGCGGGCGATGCGCTGGCCGGGCTGTGGTACCTGGTTGCGCTGCTCGGCCTGGCCGCGGTGGTCGGGCTGGTGT
TTCTGCCGGAGGTGCGCAACCGCGCGCTGGAGCGCTGA

Upstream 100 bases:

>100_bases
CCGGTGTCCGCCGCGTAGCACGCGCGTTTTCGCCCGACCGGCGCGCCCTTTCCGGGATAATGCGGGCCCCTCGTCCCGTG
CCGCTGCGGCGCTTCGTCCG

Downstream 100 bases:

>100_bases
GCCGCCTCGCTTTGGGCGTGATTTCCGTCACGCTGCCCGCGGTAAACACGAGTAAGCCGAAAGTACTAAAAGAAAGATAA
TCGGCGCTTGCGGGCAGAGG

Product: putative sugar transport protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTRPMPSPPESTASAVPADSDGHSAPDMPGAGQVQRKVAVAACFGTFLEWYDFLTFASLATYFGTLFFPPDDPVTALLAS
LGTFGVGMVVRPLGAALFGSLGDRYGRRAIFLATIVLMGGSTFLVGLLPTYASIGLFAPLLLLVLRMLQGLSVGGEIGGA
AVYLTEHAPATRRGAYTSVLQLMGPLGMLASTLQIVALQAVLSEAEFRDWGWRVPFLISIVLLAVSIKSRLSLHESPVFR
RLREQNALAKTPLRECFRDRQTLGRMALLFFCISSGGSLLFFSSQVYTAVFLKNVVKLDAATASGLVMVATVVLFPLTVL
CGWLSDRIGRRPVLLAGLVLGALTVLPVFHGLLEHGNPALARFNASVAVELAGEQCRYDPFAGVRNDCERYQELFARLGV
VHQQREGALAVKVGGQTIAGYQPEAVTRALVEAGRPAQADPAQVNRPALIALLLVLVVALACITGPQTATLAELFPARTR
YSAVALPHNLAAGWIGGLSPFMVTWLSVRAGDALAGLWYLVALLGLAAVVGLVFLPEVRNRALER

Sequences:

>Translated_545_residues
MTRPMPSPPESTASAVPADSDGHSAPDMPGAGQVQRKVAVAACFGTFLEWYDFLTFASLATYFGTLFFPPDDPVTALLAS
LGTFGVGMVVRPLGAALFGSLGDRYGRRAIFLATIVLMGGSTFLVGLLPTYASIGLFAPLLLLVLRMLQGLSVGGEIGGA
AVYLTEHAPATRRGAYTSVLQLMGPLGMLASTLQIVALQAVLSEAEFRDWGWRVPFLISIVLLAVSIKSRLSLHESPVFR
RLREQNALAKTPLRECFRDRQTLGRMALLFFCISSGGSLLFFSSQVYTAVFLKNVVKLDAATASGLVMVATVVLFPLTVL
CGWLSDRIGRRPVLLAGLVLGALTVLPVFHGLLEHGNPALARFNASVAVELAGEQCRYDPFAGVRNDCERYQELFARLGV
VHQQREGALAVKVGGQTIAGYQPEAVTRALVEAGRPAQADPAQVNRPALIALLLVLVVALACITGPQTATLAELFPARTR
YSAVALPHNLAAGWIGGLSPFMVTWLSVRAGDALAGLWYLVALLGLAAVVGLVFLPEVRNRALER
>Mature_544_residues
TRPMPSPPESTASAVPADSDGHSAPDMPGAGQVQRKVAVAACFGTFLEWYDFLTFASLATYFGTLFFPPDDPVTALLASL
GTFGVGMVVRPLGAALFGSLGDRYGRRAIFLATIVLMGGSTFLVGLLPTYASIGLFAPLLLLVLRMLQGLSVGGEIGGAA
VYLTEHAPATRRGAYTSVLQLMGPLGMLASTLQIVALQAVLSEAEFRDWGWRVPFLISIVLLAVSIKSRLSLHESPVFRR
LREQNALAKTPLRECFRDRQTLGRMALLFFCISSGGSLLFFSSQVYTAVFLKNVVKLDAATASGLVMVATVVLFPLTVLC
GWLSDRIGRRPVLLAGLVLGALTVLPVFHGLLEHGNPALARFNASVAVELAGEQCRYDPFAGVRNDCERYQELFARLGVV
HQQREGALAVKVGGQTIAGYQPEAVTRALVEAGRPAQADPAQVNRPALIALLLVLVVALACITGPQTATLAELFPARTRY
SAVALPHNLAAGWIGGLSPFMVTWLSVRAGDALAGLWYLVALLGLAAVVGLVFLPEVRNRALER

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=324, Percent_Identity=35.4938271604938, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1789941, Length=353, Percent_Identity=37.6770538243626, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788292, Length=324, Percent_Identity=35.8024691358025, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788942, Length=348, Percent_Identity=34.1954022988506, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87082231, Length=294, Percent_Identity=22.7891156462585, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082404, Length=319, Percent_Identity=23.5109717868339, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 58098; Mature: 57967

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPMPSPPESTASAVPADSDGHSAPDMPGAGQVQRKVAVAACFGTFLEWYDFLTFASLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYFGTLFFPPDDPVTALLASLGTFGVGMVVRPLGAALFGSLGDRYGRRAIFLATIVLMGG
HHHCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
STFLVGLLPTYASIGLFAPLLLLVLRMLQGLSVGGEIGGAAVYLTEHAPATRRGAYTSVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
QLMGPLGMLASTLQIVALQAVLSEAEFRDWGWRVPFLISIVLLAVSIKSRLSLHESPVFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLREQNALAKTPLRECFRDRQTLGRMALLFFCISSGGSLLFFSSQVYTAVFLKNVVKLDA
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
ATASGLVMVATVVLFPLTVLCGWLSDRIGRRPVLLAGLVLGALTVLPVFHGLLEHGNPAL
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARFNASVAVELAGEQCRYDPFAGVRNDCERYQELFARLGVVHQQREGALAVKVGGQTIAG
HHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEECC
YQPEAVTRALVEAGRPAQADPAQVNRPALIALLLVLVVALACITGPQTATLAELFPARTR
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
YSAVALPHNLAAGWIGGLSPFMVTWLSVRAGDALAGLWYLVALLGLAAVVGLVFLPEVRN
HHHEECCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALER
HHCCC
>Mature Secondary Structure 
TRPMPSPPESTASAVPADSDGHSAPDMPGAGQVQRKVAVAACFGTFLEWYDFLTFASLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYFGTLFFPPDDPVTALLASLGTFGVGMVVRPLGAALFGSLGDRYGRRAIFLATIVLMGG
HHHCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
STFLVGLLPTYASIGLFAPLLLLVLRMLQGLSVGGEIGGAAVYLTEHAPATRRGAYTSVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
QLMGPLGMLASTLQIVALQAVLSEAEFRDWGWRVPFLISIVLLAVSIKSRLSLHESPVFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLREQNALAKTPLRECFRDRQTLGRMALLFFCISSGGSLLFFSSQVYTAVFLKNVVKLDA
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
ATASGLVMVATVVLFPLTVLCGWLSDRIGRRPVLLAGLVLGALTVLPVFHGLLEHGNPAL
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARFNASVAVELAGEQCRYDPFAGVRNDCERYQELFARLGVVHQQREGALAVKVGGQTIAG
HHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEECC
YQPEAVTRALVEAGRPAQADPAQVNRPALIALLLVLVVALACITGPQTATLAELFPARTR
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
YSAVALPHNLAAGWIGGLSPFMVTWLSVRAGDALAGLWYLVALLGLAAVVGLVFLPEVRN
HHHEECCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALER
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]