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The map label for this gene is trmL [H]

Identifier: 119899105

GI number: 119899105

Start: 3095339

End: 3095872

Strand: Reverse

Name: trmL [H]

Synonym: azo2815

Alternate gene names: 119899105

Gene position: 3095872-3095339 (Counterclockwise)

Preceding gene: 119899106

Following gene: 119899098

Centisome position: 70.75

GC content: 67.79

Gene sequence:

>534_bases
GTGCGCGCTTTCCGCGCCGCGGAAGCACGACAGCGCGCCATGTTCCACATCATCCTGTTCGAACCCGAAATTCCGCCGAA
CACCGGCAACATCATCCGCCTGGCGGCCAATACCGGATGCCACCTTCACCTGGTGGAGCCGCTCGGTTTTGTGCTGACCG
ACAAGGCGCTCGCCCGCGCCGGGCTGGACTACCACGACCTCGCCCGCGTGACGGTGCACACGGACTGGCCGGCGTGCCGC
GCCTTCCTCGGCGAGCGTCGCGTCTTTGCACTGACCACGCGCGCGCAGCGCCGCCACGACACGGTGGATTTCCGGCCGGG
TGACGCTTTCCTGTTCGGTCCCGAGAGCCGCGGACTGCCGCCGGAACTGCGCGAAAGCCTGCCGCCCGAGCAGCGCCTGC
GACTGCCGATGGTTCCGTCCAACCGCAGCATGAACCTGTCTAACGCAGTGGCGGTGATGGTGTTCGAAGCGTGGCGGCAG
CAGGACTTCGTCGGCGCAGGAGACGGGCCGCAAAGCCTGCCCGTCGAACTCTGA

Upstream 100 bases:

>100_bases
CGCCACGTTGGGCGAAGCGGCGCGCATTCTCAAGCGGCGGGGTGCGGAGCGGGTCGACAATCTCGTCATCGCCCGCACAC
CCCCGCCGGGCTGAGCTACA

Downstream 100 bases:

>100_bases
AGGGGGGCGCGCGCTTATTCCCGCTTGGGGTCGCGTGCGAGCAGGCGCTCGACCGCGTCGCGCGCGGCCAGGCCGCCATG
CAGCAGCGCATCCACGGCTT

Product: tRNA/rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MRAFRAAEARQRAMFHIILFEPEIPPNTGNIIRLAANTGCHLHLVEPLGFVLTDKALARAGLDYHDLARVTVHTDWPACR
AFLGERRVFALTTRAQRRHDTVDFRPGDAFLFGPESRGLPPELRESLPPEQRLRLPMVPSNRSMNLSNAVAVMVFEAWRQ
QDFVGAGDGPQSLPVEL

Sequences:

>Translated_177_residues
MRAFRAAEARQRAMFHIILFEPEIPPNTGNIIRLAANTGCHLHLVEPLGFVLTDKALARAGLDYHDLARVTVHTDWPACR
AFLGERRVFALTTRAQRRHDTVDFRPGDAFLFGPESRGLPPELRESLPPEQRLRLPMVPSNRSMNLSNAVAVMVFEAWRQ
QDFVGAGDGPQSLPVEL
>Mature_177_residues
MRAFRAAEARQRAMFHIILFEPEIPPNTGNIIRLAANTGCHLHLVEPLGFVLTDKALARAGLDYHDLARVTVHTDWPACR
AFLGERRVFALTTRAQRRHDTVDFRPGDAFLFGPESRGLPPELRESLPPEQRLRLPMVPSNRSMNLSNAVAVMVFEAWRQ
QDFVGAGDGPQSLPVEL

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=57.6923076923077, Blast_Score=186, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19875; Mature: 19875

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAFRAAEARQRAMFHIILFEPEIPPNTGNIIRLAANTGCHLHLVEPLGFVLTDKALARA
CCCCHHHHHHCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHC
GLDYHDLARVTVHTDWPACRAFLGERRVFALTTRAQRRHDTVDFRPGDAFLFGPESRGLP
CCCHHHEEEEEEECCCHHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCEEEECCCCCCCC
PELRESLPPEQRLRLPMVPSNRSMNLSNAVAVMVFEAWRQQDFVGAGDGPQSLPVEL
HHHHHCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAFRAAEARQRAMFHIILFEPEIPPNTGNIIRLAANTGCHLHLVEPLGFVLTDKALARA
CCCCHHHHHHCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHC
GLDYHDLARVTVHTDWPACRAFLGERRVFALTTRAQRRHDTVDFRPGDAFLFGPESRGLP
CCCHHHEEEEEEECCCHHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCEEEECCCCCCCC
PELRESLPPEQRLRLPMVPSNRSMNLSNAVAVMVFEAWRQQDFVGAGDGPQSLPVEL
HHHHHCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA