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The map label for this gene is pyrG

Identifier: 119898437

GI number: 119898437

Start: 2347810

End: 2349450

Strand: Reverse

Name: pyrG

Synonym: azo2146

Alternate gene names: 119898437

Gene position: 2349450-2347810 (Counterclockwise)

Preceding gene: 119898439

Following gene: 119898436

Centisome position: 53.69

GC content: 62.71

Gene sequence:

>1641_bases
ATGACCAAATACGTCTTCGTTACTGGCGGTGTCGTGTCCTCTCTGGGCAAGGGCATCGCAGCCGCCTCCCTCGGCGCCAT
TCTCGAATCCCGCGGCATCAAGGTGACCCACCTCAAACTGGATCCGTACATCAACGTCGATCCGGGCACCATGAGCCCCT
TCCAGCACGGTGAAGTGTTCGTCACCGAAGACGGCGCGGAAACCGACCTCGATCTGGGCCACTACGAGCGCTTCACCAGC
GCCAAGATGAGCAAGCGCAACAACTTCACCACCGGCCAGATCTACGAGGCGGTGATCAAGAAGGAGCGGCGCGGCGAATA
TCTCGGCAAGACGGTTCAGGTCATTCCGCACATCACCGATGAGATCAAGAGCTACGTCAAGCGTGGCGCCGAAGGCGCCG
ATGTGGCGATCGTTGAAGTCGGCGGCACTGTCGGCGACATCGAGTCGCTGCCCTTCCTCGAAGCCATCCGCCAGATGGGC
ATCGAAGAAGGCCGCAACGCCACCTGTTTCATCCACCTCACGCTGCTGCCTTACATTCCGACCGCGGGCGAGCTGAAGAC
CAAGCCGACCCAGCACTCGGTGAAGGAGTTGCGCGAGATCGGCATCCAGCCCGACATCCTGCTGTGCCGCGCCGACCGCT
CCATTCCGGCGGACGAGCGCCGCAAGATCGCGCTGTTCTGCAACGTGATGCCGGAAGCGGTGATCGAGTGCCTCGATGCC
GACTCCATCTACAAGATTCCCGGTCAGCTGCACGACCAGATGCTGGACGAGATCGTCTGCCACAAGCTGGGCATCCTTGC
CCGCGCGGCCGATCTTTCCGTCTGGGAGAACCTGATCCGCGCGCTGGAAAACCCGAAGCAGGCGGTCGATATCGCCTTCG
TCGGCAAGTACGTCGATCTGACCGAGTCGTACAAGTCGCTGATCGAGGCGCTCAACCACGCCGGCATGCACACCGAATCG
AAGGTGAACATCCACTACCTCGATTCCGAAGAGATCGAACGCAGCGGCTGCGCGGTGCTCGAGAAGATGGATGCCATTCT
GGTGCCCGGCGGCTTCGGCAAGCGCGGCACCGAAGGCAAGATCGCCGCGATCCGCTATGCGCGCGAGAACAAGGTGCCGT
ATCTCGGCATCTGTCTGGGCATGCAGCTGGCGGTCGTGGAATTCGCCCGCGACGTGGCCGGCATGGCGGAGGCGCACTCC
ACCGAGTTCGAGCGCGAAACCCCGTTCCCGGTCATCGGCCTGATCACCGAATGGGCTGATCGCAGCGGCAAGGTCGAAAA
GCGCAGCGAAGACTCCGATCTCGGCGGCACGATGCGGCTCGGCGGCCAGGTGTGCAAGCTGGCCGACGGCACGCTCGCAC
GTGAGGTCTACGGTTCTGGCGAGATCATGGAGCGTCACCGCCATCGCTATGAAGTGAACAACACGCTGCTGGCCCAGCTC
GAAGAGAAGGGGTTGGTGGTTTCCGGGCGCGCCCCGGTGACCGACCTGTGCGAAATGGTCGAACTGCCCGCCGATGTTCA
TCCCTGGTTCGTCGGTTGCCAGTTCCATCCGGAATTCACCTCCAATCCGCGCAAGGGGCATCCGCTGTTCACCGCCTACG
TCAAGGCAGCGATCGCCCGCCATAGCGCGTCACGCGGCTGA

Upstream 100 bases:

>100_bases
ATCAATGCCGGAAACGTGTTCGCCCCCCGTAACGGGTGGCCTCGCTTCCGGGGCACGGGGCTCTTCGGTTATCATCGCGC
TTTCCCGCAGCACAGTTCCC

Downstream 100 bases:

>100_bases
AGGAGCAACGCATGAATCTGTGTGGATTCGAGGTCGGCCTCGACAAGCCGGTTTTCCTGATTGCCGGCCCGTGCGTGATC
GAGTCGCGCGAGATGGCGTT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTS
AKMSKRNNFTTGQIYEAVIKKERRGEYLGKTVQVIPHITDEIKSYVKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
IEEGRNATCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPADERRKIALFCNVMPEAVIECLDA
DSIYKIPGQLHDQMLDEIVCHKLGILARAADLSVWENLIRALENPKQAVDIAFVGKYVDLTESYKSLIEALNHAGMHTES
KVNIHYLDSEEIERSGCAVLEKMDAILVPGGFGKRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMAEAHS
TEFERETPFPVIGLITEWADRSGKVEKRSEDSDLGGTMRLGGQVCKLADGTLAREVYGSGEIMERHRHRYEVNNTLLAQL
EEKGLVVSGRAPVTDLCEMVELPADVHPWFVGCQFHPEFTSNPRKGHPLFTAYVKAAIARHSASRG

Sequences:

>Translated_546_residues
MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTS
AKMSKRNNFTTGQIYEAVIKKERRGEYLGKTVQVIPHITDEIKSYVKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
IEEGRNATCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPADERRKIALFCNVMPEAVIECLDA
DSIYKIPGQLHDQMLDEIVCHKLGILARAADLSVWENLIRALENPKQAVDIAFVGKYVDLTESYKSLIEALNHAGMHTES
KVNIHYLDSEEIERSGCAVLEKMDAILVPGGFGKRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMAEAHS
TEFERETPFPVIGLITEWADRSGKVEKRSEDSDLGGTMRLGGQVCKLADGTLAREVYGSGEIMERHRHRYEVNNTLLAQL
EEKGLVVSGRAPVTDLCEMVELPADVHPWFVGCQFHPEFTSNPRKGHPLFTAYVKAAIARHSASRG
>Mature_545_residues
TKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSA
KMSKRNNFTTGQIYEAVIKKERRGEYLGKTVQVIPHITDEIKSYVKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMGI
EEGRNATCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPADERRKIALFCNVMPEAVIECLDAD
SIYKIPGQLHDQMLDEIVCHKLGILARAADLSVWENLIRALENPKQAVDIAFVGKYVDLTESYKSLIEALNHAGMHTESK
VNIHYLDSEEIERSGCAVLEKMDAILVPGGFGKRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMAEAHST
EFERETPFPVIGLITEWADRSGKVEKRSEDSDLGGTMRLGGQVCKLADGTLAREVYGSGEIMERHRHRYEVNNTLLAQLE
EKGLVVSGRAPVTDLCEMVELPADVHPWFVGCQFHPEFTSNPRKGHPLFTAYVKAAIARHSASRG

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=565, Percent_Identity=44.2477876106195, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI28559083, Length=565, Percent_Identity=44.2477876106195, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI221316689, Length=565, Percent_Identity=44.2477876106195, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI148491070, Length=564, Percent_Identity=45.0354609929078, Blast_Score=473, Evalue=1e-133,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=64.7388059701492, Blast_Score=709, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=612, Percent_Identity=38.8888888888889, Blast_Score=420, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=42.280701754386, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6319432, Length=568, Percent_Identity=40.8450704225352, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24664469, Length=562, Percent_Identity=43.9501779359431, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI21357815, Length=507, Percent_Identity=42.4063116370809, Blast_Score=387, Evalue=1e-107,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_AZOSB (A1K7F8)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933650.1
- ProteinModelPortal:   A1K7F8
- SMR:   A1K7F8
- STRING:   A1K7F8
- GeneID:   4609103
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2146
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   IIHAGAN
- PhylomeDB:   A1K7F8
- ProtClustDB:   PRK05380
- BioCyc:   ASP62928:AZO2146-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60114; Mature: 59982

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 378-378 ACT_SITE 516-516 ACT_SITE 518-518

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTSAKMSKRNNFTTGQIYEAVIKKERRGEYLGKTVQVIPHITD
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
EIKSYVKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMGIEEGRNATCFIHLTLLPYIP
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCC
TAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPADERRKIALFCNVMPEAVIECLDA
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHCC
DSIYKIPGQLHDQMLDEIVCHKLGILARAADLSVWENLIRALENPKQAVDIAFVGKYVDL
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHEEHHHHHHH
TESYKSLIEALNHAGMHTESKVNIHYLDSEEIERSGCAVLEKMDAILVPGGFGKRGTEGK
HHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
IAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMAEAHSTEFERETPFPVIGLITEWAD
EEEEEEHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHC
RSGKVEKRSEDSDLGGTMRLGGQVCKLADGTLAREVYGSGEIMERHRHRYEVNNTLLAQL
CCCCCCCCCCCCCCCCEEHHCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCHHHHHHH
EEKGLVVSGRAPVTDLCEMVELPADVHPWFVGCQFHPEFTSNPRKGHPLFTAYVKAAIAR
HHCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
HSASRG
HCCCCC
>Mature Secondary Structure 
TKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTSAKMSKRNNFTTGQIYEAVIKKERRGEYLGKTVQVIPHITD
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
EIKSYVKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMGIEEGRNATCFIHLTLLPYIP
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCC
TAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPADERRKIALFCNVMPEAVIECLDA
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHCC
DSIYKIPGQLHDQMLDEIVCHKLGILARAADLSVWENLIRALENPKQAVDIAFVGKYVDL
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHEEHHHHHHH
TESYKSLIEALNHAGMHTESKVNIHYLDSEEIERSGCAVLEKMDAILVPGGFGKRGTEGK
HHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
IAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMAEAHSTEFERETPFPVIGLITEWAD
EEEEEEHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHC
RSGKVEKRSEDSDLGGTMRLGGQVCKLADGTLAREVYGSGEIMERHRHRYEVNNTLLAQL
CCCCCCCCCCCCCCCCEEHHCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCHHHHHHH
EEKGLVVSGRAPVTDLCEMVELPADVHPWFVGCQFHPEFTSNPRKGHPLFTAYVKAAIAR
HHCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
HSASRG
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA