Click here to switch to the map view.

The map label for this gene is kdsA

Identifier: 119898436

GI number: 119898436

Start: 2346959

End: 2347798

Strand: Reverse

Name: kdsA

Synonym: azo2145

Alternate gene names: 119898436

Gene position: 2347798-2346959 (Counterclockwise)

Preceding gene: 119898437

Following gene: 119898435

Centisome position: 53.65

GC content: 64.29

Gene sequence:

>840_bases
ATGAATCTGTGTGGATTCGAGGTCGGCCTCGACAAGCCGGTTTTCCTGATTGCCGGCCCGTGCGTGATCGAGTCGCGCGA
GATGGCGTTCGAAACCGCCGGCGCGCTCAAGGACATCTGCGCTGAAGTCGGCATTCCCTTCATCTACAAGTCGTCCTACG
ACAAGGCCAACCGCAGCTCGGGCAAGTCCTATCGCGGCATGGGCATGGAGAAGGGACTGGACATCCTCGCGGACGTCAAG
AAGCAGCTCGGCGTGCCGGTGCTGACCGACGTGCATTCGATCGAGGAGATCCCGGCCGTCGCGGCGGTGGTCGATGTGCT
GCAGACCCCCGCCTTTCTGTGTCGCCAAACCGACTTCATCCACGCGGTGGCGTCCTGTGGCCGCCCGGTGAATATCAAGA
AAGGCCAGTTTCTCGCGCCCGGCGACATGAAGAACGTGGTCGACAAGGCCCGGGAAGCCAACGGCGGCGCTGACAACATC
ATGGTGTGCGAGCGCGGCGCGTCCTTCGGCTACAACAACCTGGTGTCCGACATGCGCTCCCTCGCGATCATGCGCGAGAC
CGGCTGCCCGGTGGTGTTCGATGCCACCCACTCGGTGCAACTTCCGGGCGGGCAGGGCACGGCATCGGGTGGTCAGCGCG
AATTCGTACCCGTCCTCGCCAGGGCGGCTGTCGCGGTCGGCGTGGCGGGTCTTTTCATGGAAACTCACCCGAATCCCGCG
CAGGCGCTTTCCGATGGCCCCAATGCCTGGCCCTTGCCGCGCATGAAGGCGTTGCTCAGCACGCTGAAGGCGATCGACGA
ACTGGTCAAGTCGCGCGGGCTGCTGGAAACCGAGGCCTGA

Upstream 100 bases:

>100_bases
AATTCACCTCCAATCCGCGCAAGGGGCATCCGCTGTTCACCGCCTACGTCAAGGCAGCGATCGCCCGCCATAGCGCGTCA
CGCGGCTGAAGGAGCAACGC

Downstream 100 bases:

>100_bases
TCCGCTTCGTGTGCCGCCCCATGTGCAGGCGCACCCTGATTTTTTCTTCCAATTCCTAATCCGTAACCGAAACAGGAACA
TTCCATGAGTGCAATCGTTG

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNLCGFEVGLDKPVFLIAGPCVIESREMAFETAGALKDICAEVGIPFIYKSSYDKANRSSGKSYRGMGMEKGLDILADVK
KQLGVPVLTDVHSIEEIPAVAAVVDVLQTPAFLCRQTDFIHAVASCGRPVNIKKGQFLAPGDMKNVVDKAREANGGADNI
MVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTASGGQREFVPVLARAAVAVGVAGLFMETHPNPA
QALSDGPNAWPLPRMKALLSTLKAIDELVKSRGLLETEA

Sequences:

>Translated_279_residues
MNLCGFEVGLDKPVFLIAGPCVIESREMAFETAGALKDICAEVGIPFIYKSSYDKANRSSGKSYRGMGMEKGLDILADVK
KQLGVPVLTDVHSIEEIPAVAAVVDVLQTPAFLCRQTDFIHAVASCGRPVNIKKGQFLAPGDMKNVVDKAREANGGADNI
MVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTASGGQREFVPVLARAAVAVGVAGLFMETHPNPA
QALSDGPNAWPLPRMKALLSTLKAIDELVKSRGLLETEA
>Mature_279_residues
MNLCGFEVGLDKPVFLIAGPCVIESREMAFETAGALKDICAEVGIPFIYKSSYDKANRSSGKSYRGMGMEKGLDILADVK
KQLGVPVLTDVHSIEEIPAVAAVVDVLQTPAFLCRQTDFIHAVASCGRPVNIKKGQFLAPGDMKNVVDKAREANGGADNI
MVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTASGGQREFVPVLARAAVAVGVAGLFMETHPNPA
QALSDGPNAWPLPRMKALLSTLKAIDELVKSRGLLETEA

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=272, Percent_Identity=47.7941176470588, Blast_Score=260, Evalue=8e-71,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_AZOSB (A1K7F7)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933649.1
- ProteinModelPortal:   A1K7F7
- SMR:   A1K7F7
- STRING:   A1K7F7
- GeneID:   4608670
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2145
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   ESREMAF
- PhylomeDB:   A1K7F7
- ProtClustDB:   PRK05198
- BioCyc:   ASP62928:AZO2145-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29607; Mature: 29607

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLCGFEVGLDKPVFLIAGPCVIESREMAFETAGALKDICAEVGIPFIYKSSYDKANRSS
CCCCCCCCCCCCCEEEEECCEEECHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCC
GKSYRGMGMEKGLDILADVKKQLGVPVLTDVHSIEEIPAVAAVVDVLQTPAFLCRQTDFI
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
HAVASCGRPVNIKKGQFLAPGDMKNVVDKAREANGGADNIMVCERGASFGYNNLVSDMRS
HHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHH
LAIMRETGCPVVFDATHSVQLPGGQGTASGGQREFVPVLARAAVAVGVAGLFMETHPNPA
HHHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QALSDGPNAWPLPRMKALLSTLKAIDELVKSRGLLETEA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNLCGFEVGLDKPVFLIAGPCVIESREMAFETAGALKDICAEVGIPFIYKSSYDKANRSS
CCCCCCCCCCCCCEEEEECCEEECHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCC
GKSYRGMGMEKGLDILADVKKQLGVPVLTDVHSIEEIPAVAAVVDVLQTPAFLCRQTDFI
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
HAVASCGRPVNIKKGQFLAPGDMKNVVDKAREANGGADNIMVCERGASFGYNNLVSDMRS
HHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHH
LAIMRETGCPVVFDATHSVQLPGGQGTASGGQREFVPVLARAAVAVGVAGLFMETHPNPA
HHHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QALSDGPNAWPLPRMKALLSTLKAIDELVKSRGLLETEA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA