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The map label for this gene is dapE

Identifier: 119898298

GI number: 119898298

Start: 2213566

End: 2214708

Strand: Direct

Name: dapE

Synonym: azo2007

Alternate gene names: 119898298

Gene position: 2213566-2214708 (Clockwise)

Preceding gene: 119898297

Following gene: 119898299

Centisome position: 50.58

GC content: 66.32

Gene sequence:

>1143_bases
ATGTCCCTCCCCGACACTCCAACGCTCGCGCTCGCATGCGAGCTGATCTCCCGTTCATCGGTCACGCCCGAGGACGCGGG
CTGCCTCCAACTCATCGCCCAACGCCTGGCGCCGCTCGGCTTCGTGTGCGAACGCATCGATATCGGCGGTGTGTCCAATC
TGTGGGCCCGGCGTGGTTCGGCCCGGCCGCTGCTGTGCTTTGCCGGCCACACCGACGTGGTTCCGACCGGGCCGCTGGAC
GCCTGGCAGTCTCCGCCGTTCGAACCCACGATTCGCGACGGACACCTGTATGGTCGCGGCGCTGCAGACATGAAGTCCTC
GCTGGCGGGTTTCGTCACTGCAATCGAGCGTTTCGTCGCCGCCCATCCGGACCACGCGGGCAGCATCGCCCTGCTGCTGA
CCTCGGACGAGGAAGGCGTGGCCACCTGCGGCACCGTTAAGGTTGTGGAAGCCCTGGCCGCGCGCGGCGAGCGGCTGGAC
TACTGCGTGGTCGGGGAACCGACCTCGGTGAAGACGCTGGGCGACATGATCAAGAACGGCCGTCGTGGCTCGCTGTCCGG
CACCCTGCGCGTCAAGGGGCGGCAGGGGCACGTCGCTTATCCGCACCTTGCGCGCAATCCGATCCACGAACTGGCTCCCG
CGCTCGCCGAGCTGGCCGCGGAACGCTGGGACGACGGCAACGAGTTCTTCCCGCCCACCACCTGGCAAGTGTCCAACATC
CATGCCGGCACCGGCGCCAACAATGTCATTCCCGGCGTGTGCGACGTGCTGTTCAACTTCCGCTTCGGTTCGGTCAGCAC
GGCCGACGCGTTGAAGGCTCGCACCCATGCCATCCTCGATCGGCATGGCCTGGATTACGAACTCGACTGGCATCTGTCCG
GCAAGCCCTTCATCACCGGACGCGGTCAACTGGTCGCCGCCCTGGGCAACGCGATCCGCGAGACCGTCGGTGTGGAAACC
GAACTATCGACCACCGGCGGCACCTCGGACGGCCGCTTCATCGCCGACATCTGCGCCGAGGTGGTCGAATTCGGGCCGGT
CAATGCGTCCATCCATCAGGTGAACGAACACATTGCGGTCGATGCGGTCGAGCCCCTGTCCAAGATCTACGAGCGCACCT
TGCGCGCCCTGCTTACTGCCTGA

Upstream 100 bases:

>100_bases
AACCGGACGGCGCCTCGTTCCGCGAGTTCACGCTGCATCTAGACGAACCTTCGCAGTAAAGGGCCGCGCCGTCCGCGGTT
CCGCCTGCCATTACAAGACC

Downstream 100 bases:

>100_bases
GGCTGTCGATGAGCCATCACGAAAACCCCGCCTGCGACGATCCCGACTGCAACGACGCGGATCACGACCACGAGCACGAG
CACGGTCCGCTGGCCGAACT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSLPDTPTLALACELISRSSVTPEDAGCLQLIAQRLAPLGFVCERIDIGGVSNLWARRGSARPLLCFAGHTDVVPTGPLD
AWQSPPFEPTIRDGHLYGRGAADMKSSLAGFVTAIERFVAAHPDHAGSIALLLTSDEEGVATCGTVKVVEALAARGERLD
YCVVGEPTSVKTLGDMIKNGRRGSLSGTLRVKGRQGHVAYPHLARNPIHELAPALAELAAERWDDGNEFFPPTTWQVSNI
HAGTGANNVIPGVCDVLFNFRFGSVSTADALKARTHAILDRHGLDYELDWHLSGKPFITGRGQLVAALGNAIRETVGVET
ELSTTGGTSDGRFIADICAEVVEFGPVNASIHQVNEHIAVDAVEPLSKIYERTLRALLTA

Sequences:

>Translated_380_residues
MSLPDTPTLALACELISRSSVTPEDAGCLQLIAQRLAPLGFVCERIDIGGVSNLWARRGSARPLLCFAGHTDVVPTGPLD
AWQSPPFEPTIRDGHLYGRGAADMKSSLAGFVTAIERFVAAHPDHAGSIALLLTSDEEGVATCGTVKVVEALAARGERLD
YCVVGEPTSVKTLGDMIKNGRRGSLSGTLRVKGRQGHVAYPHLARNPIHELAPALAELAAERWDDGNEFFPPTTWQVSNI
HAGTGANNVIPGVCDVLFNFRFGSVSTADALKARTHAILDRHGLDYELDWHLSGKPFITGRGQLVAALGNAIRETVGVET
ELSTTGGTSDGRFIADICAEVVEFGPVNASIHQVNEHIAVDAVEPLSKIYERTLRALLTA
>Mature_379_residues
SLPDTPTLALACELISRSSVTPEDAGCLQLIAQRLAPLGFVCERIDIGGVSNLWARRGSARPLLCFAGHTDVVPTGPLDA
WQSPPFEPTIRDGHLYGRGAADMKSSLAGFVTAIERFVAAHPDHAGSIALLLTSDEEGVATCGTVKVVEALAARGERLDY
CVVGEPTSVKTLGDMIKNGRRGSLSGTLRVKGRQGHVAYPHLARNPIHELAPALAELAAERWDDGNEFFPPTTWQVSNIH
AGTGANNVIPGVCDVLFNFRFGSVSTADALKARTHAILDRHGLDYELDWHLSGKPFITGRGQLVAALGNAIRETVGVETE
LSTTGGTSDGRFIADICAEVVEFGPVNASIHQVNEHIAVDAVEPLSKIYERTLRALLTA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Homo sapiens, GI21071039, Length=134, Percent_Identity=31.3432835820896, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788816, Length=372, Percent_Identity=56.989247311828, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1790395, Length=389, Percent_Identity=26.2210796915167, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551016, Length=242, Percent_Identity=28.5123966942149, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_AZOSB (A1K719)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933511.1
- ProteinModelPortal:   A1K719
- SMR:   A1K719
- STRING:   A1K719
- MEROPS:   M20.010
- GeneID:   4607173
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2007
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   A1K719
- ProtClustDB:   PRK13009
- BioCyc:   ASP62928:AZO2007-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40508; Mature: 40377

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 72-72 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPDTPTLALACELISRSSVTPEDAGCLQLIAQRLAPLGFVCERIDIGGVSNLWARRGS
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHCCC
ARPLLCFAGHTDVVPTGPLDAWQSPPFEPTIRDGHLYGRGAADMKSSLAGFVTAIERFVA
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHH
AHPDHAGSIALLLTSDEEGVATCGTVKVVEALAARGERLDYCVVGEPTSVKTLGDMIKNG
CCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCC
RRGSLSGTLRVKGRQGHVAYPHLARNPIHELAPALAELAAERWDDGNEFFPPTTWQVSNI
CCCCCCEEEEEECCCCCEECCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECE
HAGTGANNVIPGVCDVLFNFRFGSVSTADALKARTHAILDRHGLDYELDWHLSGKPFITG
ECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECC
RGQLVAALGNAIRETVGVETELSTTGGTSDGRFIADICAEVVEFGPVNASIHQVNEHIAV
CCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
DAVEPLSKIYERTLRALLTA
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLPDTPTLALACELISRSSVTPEDAGCLQLIAQRLAPLGFVCERIDIGGVSNLWARRGS
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHCCC
ARPLLCFAGHTDVVPTGPLDAWQSPPFEPTIRDGHLYGRGAADMKSSLAGFVTAIERFVA
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHH
AHPDHAGSIALLLTSDEEGVATCGTVKVVEALAARGERLDYCVVGEPTSVKTLGDMIKNG
CCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCC
RRGSLSGTLRVKGRQGHVAYPHLARNPIHELAPALAELAAERWDDGNEFFPPTTWQVSNI
CCCCCCEEEEEECCCCCEECCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECE
HAGTGANNVIPGVCDVLFNFRFGSVSTADALKARTHAILDRHGLDYELDWHLSGKPFITG
ECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECC
RGQLVAALGNAIRETVGVETELSTTGGTSDGRFIADICAEVVEFGPVNASIHQVNEHIAV
CCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
DAVEPLSKIYERTLRALLTA
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA