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The map label for this gene is pilT [H]

Identifier: 119898297

GI number: 119898297

Start: 2212337

End: 2213524

Strand: Direct

Name: pilT [H]

Synonym: azo2006

Alternate gene names: 119898297

Gene position: 2212337-2213524 (Clockwise)

Preceding gene: 119898296

Following gene: 119898298

Centisome position: 50.56

GC content: 63.22

Gene sequence:

>1188_bases
ATGATTTTCGACAAGCTGTTCCAGCTGATGGCCGAGAAGCAGGCCTCGGACATGTTCATCTCGGCCGGGGCGCCGATCCA
CATCAAGATCCAGGGCCATTCGCTGCCGATCAACCAGCAGCTCATGGACCCCGCCATGATCCAGCGCATGGTGCAGGAGC
TGCTCACGCCCGAGCAGGTGTCACGGGTCGAGAAGGAAAAGGAGCTGAACCTCTCGTTCGGCCGCCGCGACCTCGGCAAC
TTCCGCGTCAACGTGTTCTGGCAGCGCAACTCGATGGCGCTGGTGGTGCGCTTCATCCAGAGCGACATTCCGACCATCGA
CACGCTCGGCCTGCCGCCGGTGCTGTCCGAGGTGGTAATGGAAAAGCGCGGGCTGGTGCTCGTGGTGGGGGCAACCGGCT
CCGGCAAGTCCACCACGCTGGCGTCGATGATCGACCACCGCATCCGCAACAAATCCGGCCACGTGCTGACGGTTGAGGAC
CCGATCGAATACCTGTTCCAGCACCGCAAGTCGGTGGTGAACCAGCGCGAGGTCGGGATCGATACGCTGAGCTGGCATGA
AGCGTTGCGCAACGCGATGCGGCAGGCGCCGGACTGTATTCTGATCGGCGAAATCCGTGACCGCGAGACGATGCAGGCTG
CGCTCGCCTATTCCCAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCGTACCACGCGCTCAACCGCATC
ATCAACTTCTTCCCGCTGGAAAACCGCTCGCTGCTGTACCTCGACCTGGCGGTGGCCTTGCGCTGCATCATCTCCCAGCG
CCTGGTGCGCAAGCCCGACGGCAAGCGCATTCCCACCGTGGAAATCCTGATGAACACCCGCCACGTCGCGGAACTGGTGG
AGCGAGGCGAGCTGAACGAGATCAAGGAGGCGATGGAGCAAAGCCTCGCCCCGGGCTCGCAGACCTTCGAGCAGGACCTG
TTCCGCCTGTACCACGAAAAGGTGATCACGCTCGACGAGGCGTTGGCCAACTCCGATTCGCCGACGAACCTGTCCTGGCT
GATCAATAACGCCCAGTTCGGGAACCTGCCCGACCCGGCCCAGGCCCAGGCCAATGCCGCCCGTACGGTGACCGATTTCG
AACGCACCCAACCGGACGGCGCCTCGTTCCGCGAGTTCACGCTGCATCTAGACGAACCTTCGCAGTAA

Upstream 100 bases:

>100_bases
GCTGCGCGGCGCCTGATCCACCGGGGGCGGCGTTGCCGCCCCGCCCTGCCCCTCGCCGTGGCCCGCCCACGGCCCTCAGT
CGGAACGGAGTGCGATACCC

Downstream 100 bases:

>100_bases
AGGGCCGCGCCGTCCGCGGTTCCGCCTGCCATTACAAGACCATGTCCCTCCCCGACACTCCAACGCTCGCGCTCGCATGC
GAGCTGATCTCCCGTTCATC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MIFDKLFQLMAEKQASDMFISAGAPIHIKIQGHSLPINQQLMDPAMIQRMVQELLTPEQVSRVEKEKELNLSFGRRDLGN
FRVNVFWQRNSMALVVRFIQSDIPTIDTLGLPPVLSEVVMEKRGLVLVVGATGSGKSTTLASMIDHRIRNKSGHVLTVED
PIEYLFQHRKSVVNQREVGIDTLSWHEALRNAMRQAPDCILIGEIRDRETMQAALAYSQTGHLCLATLHANNAYHALNRI
INFFPLENRSLLYLDLAVALRCIISQRLVRKPDGKRIPTVEILMNTRHVAELVERGELNEIKEAMEQSLAPGSQTFEQDL
FRLYHEKVITLDEALANSDSPTNLSWLINNAQFGNLPDPAQAQANAARTVTDFERTQPDGASFREFTLHLDEPSQ

Sequences:

>Translated_395_residues
MIFDKLFQLMAEKQASDMFISAGAPIHIKIQGHSLPINQQLMDPAMIQRMVQELLTPEQVSRVEKEKELNLSFGRRDLGN
FRVNVFWQRNSMALVVRFIQSDIPTIDTLGLPPVLSEVVMEKRGLVLVVGATGSGKSTTLASMIDHRIRNKSGHVLTVED
PIEYLFQHRKSVVNQREVGIDTLSWHEALRNAMRQAPDCILIGEIRDRETMQAALAYSQTGHLCLATLHANNAYHALNRI
INFFPLENRSLLYLDLAVALRCIISQRLVRKPDGKRIPTVEILMNTRHVAELVERGELNEIKEAMEQSLAPGSQTFEQDL
FRLYHEKVITLDEALANSDSPTNLSWLINNAQFGNLPDPAQAQANAARTVTDFERTQPDGASFREFTLHLDEPSQ
>Mature_395_residues
MIFDKLFQLMAEKQASDMFISAGAPIHIKIQGHSLPINQQLMDPAMIQRMVQELLTPEQVSRVEKEKELNLSFGRRDLGN
FRVNVFWQRNSMALVVRFIQSDIPTIDTLGLPPVLSEVVMEKRGLVLVVGATGSGKSTTLASMIDHRIRNKSGHVLTVED
PIEYLFQHRKSVVNQREVGIDTLSWHEALRNAMRQAPDCILIGEIRDRETMQAALAYSQTGHLCLATLHANNAYHALNRI
INFFPLENRSLLYLDLAVALRCIISQRLVRKPDGKRIPTVEILMNTRHVAELVERGELNEIKEAMEQSLAPGSQTFEQDL
FRLYHEKVITLDEALANSDSPTNLSWLINNAQFGNLPDPAQAQANAARTVTDFERTQPDGASFREFTLHLDEPSQ

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=33.8557993730408, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789723, Length=217, Percent_Identity=35.0230414746544, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786296, Length=199, Percent_Identity=36.1809045226131, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 44624; Mature: 44624

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDKLFQLMAEKQASDMFISAGAPIHIKIQGHSLPINQQLMDPAMIQRMVQELLTPEQV
CCHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHCCHHHH
SRVEKEKELNLSFGRRDLGNFRVNVFWQRNSMALVVRFIQSDIPTIDTLGLPPVLSEVVM
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EKRGLVLVVGATGSGKSTTLASMIDHRIRNKSGHVLTVEDPIEYLFQHRKSVVNQREVGI
HCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
DTLSWHEALRNAMRQAPDCILIGEIRDRETMQAALAYSQTGHLCLATLHANNAYHALNRI
CHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
INFFPLENRSLLYLDLAVALRCIISQRLVRKPDGKRIPTVEILMNTRHVAELVERGELNE
HHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
IKEAMEQSLAPGSQTFEQDLFRLYHEKVITLDEALANSDSPTNLSWLINNAQFGNLPDPA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHH
QAQANAARTVTDFERTQPDGASFREFTLHLDEPSQ
HHHHCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MIFDKLFQLMAEKQASDMFISAGAPIHIKIQGHSLPINQQLMDPAMIQRMVQELLTPEQV
CCHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHCCHHHH
SRVEKEKELNLSFGRRDLGNFRVNVFWQRNSMALVVRFIQSDIPTIDTLGLPPVLSEVVM
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EKRGLVLVVGATGSGKSTTLASMIDHRIRNKSGHVLTVEDPIEYLFQHRKSVVNQREVGI
HCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
DTLSWHEALRNAMRQAPDCILIGEIRDRETMQAALAYSQTGHLCLATLHANNAYHALNRI
CHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
INFFPLENRSLLYLDLAVALRCIISQRLVRKPDGKRIPTVEILMNTRHVAELVERGELNE
HHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
IKEAMEQSLAPGSQTFEQDLFRLYHEKVITLDEALANSDSPTNLSWLINNAQFGNLPDPA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHH
QAQANAARTVTDFERTQPDGASFREFTLHLDEPSQ
HHHHCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]