Click here to switch to the map view.

The map label for this gene is bioF

Identifier: 119898180

GI number: 119898180

Start: 2074477

End: 2075649

Strand: Direct

Name: bioF

Synonym: azo1889

Alternate gene names: 119898180

Gene position: 2074477-2075649 (Clockwise)

Preceding gene: 119898179

Following gene: 119898181

Centisome position: 47.41

GC content: 71.61

Gene sequence:

>1173_bases
ATGTCCGCCCTGCTGGACCGCCTGCGCACCGACCTCTCCGCGCTTGCCGGCCAGGGCCTGCGGCGGGTGCGCCGCAGCAA
CGCCCTGCCCTGCGCGCCGCGCGCCCTGGTCGACGGACGGGAGATGCTCGCCTTCTGTTCCAACGACTACCTTGGCCTCG
CCGCCGAACCCGCCCTGGCGACGGCGCTCGCGCAAGCGAGCAGCCGGTGGGGCGCGGGCTCGGGCGCATCGCACCTCGTC
AGCGGCCACTATGCAGTGCACGACGCGCTGGAGCACCGCCTGGCGGCCTTCGTCGGCGCCGAGCGCGCGCTCTATTTTTC
CACCGGCTACATGGCCAACGCCGGCATCGTCCCGGCGCTCGTGGGGCGGGGGGACGCGGTGTTCGCAGACCGTCTCAACC
ACGCTTCGCTGGTCGATGGCGTGCTGCTGTCGCGTGCCGACATGCACCGCTACCCGCACGGCGATCTGGCGGTGCTGGCA
CGTCAGCTCGCCGGCAGCAGCGCGGCCCGCAAGCTCATCGTCACCGATGCCGTGTTCAGCATGGACGGCGACGTGGCACC
GCTCGCCGATCTGCTCGAACTCGCCGAGCGCCACGACGCCTGGCTGATGGTGGATGACGCGCACGGCTTCGGCGTGCTGG
GGCCGCAGGGGCGCGGTGCCCTGGCGGACGCGGGGTTGCAGCACTGGCGGCTGATCTACGTCGGCACGCTGGGCAAGGCC
GCGGGCGTTTCCGGCGCGTTTGCCGCCGGTCACGGCGAACTCGTGGAATGGCTGTTGCAGAAGGCGCGCACCTACATCTT
CACGACCGGCGCACCGCCAGCGCTGGCGGAAGCGCTGCTGCTCAGCCTTGACCTGATCGAGGCCGCCGACGACCGTCGCA
CGCATCTCGCCGGCCTCATCGAACGCTTTGGCGCAGAACTGACGCTGAGCCGCTGGCAGCGCCTGCCCTCGCGCACGCCG
ATCCAGCCGATCCGCATCGGTGGCAACGAGGAAGCCCTGGCGGTCGCCCGCGCGCTGTGGGATGAAGGGTTGTGGGTGCC
CGCGATTCGTCCGCCCACGGTTCCGGCCGGCAGTGCCCGCCTGCGGGTGTCGCTGACCGCTGCCCATACCGAAGCCGACG
TGCTTCGTCTCGCGCGGACGCTGAACCGCCTGGAGCACGCCGCGCATGGTTGA

Upstream 100 bases:

>100_bases
GCAACACGGTCTACTTCATGCCGCCCTACGTGCTGGGCGAGGACGAACTGCGTCACCTCGTCGACGGCGCGGCCGCCGCG
CTAGAGCGGGCGCTGCGCTG

Downstream 100 bases:

>100_bases
GATCGGGATGCCCAGCCTGCCGCCGCTGGTGCTGTTGCACGGCTGGGGCATGACGCCGGCGGTCTGGGACGGGCTCGCAC
CGGCGCTGCGCGAGCGCTTC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSALLDRLRTDLSALAGQGLRRVRRSNALPCAPRALVDGREMLAFCSNDYLGLAAEPALATALAQASSRWGAGSGASHLV
SGHYAVHDALEHRLAAFVGAERALYFSTGYMANAGIVPALVGRGDAVFADRLNHASLVDGVLLSRADMHRYPHGDLAVLA
RQLAGSSAARKLIVTDAVFSMDGDVAPLADLLELAERHDAWLMVDDAHGFGVLGPQGRGALADAGLQHWRLIYVGTLGKA
AGVSGAFAAGHGELVEWLLQKARTYIFTTGAPPALAEALLLSLDLIEAADDRRTHLAGLIERFGAELTLSRWQRLPSRTP
IQPIRIGGNEEALAVARALWDEGLWVPAIRPPTVPAGSARLRVSLTAAHTEADVLRLARTLNRLEHAAHG

Sequences:

>Translated_390_residues
MSALLDRLRTDLSALAGQGLRRVRRSNALPCAPRALVDGREMLAFCSNDYLGLAAEPALATALAQASSRWGAGSGASHLV
SGHYAVHDALEHRLAAFVGAERALYFSTGYMANAGIVPALVGRGDAVFADRLNHASLVDGVLLSRADMHRYPHGDLAVLA
RQLAGSSAARKLIVTDAVFSMDGDVAPLADLLELAERHDAWLMVDDAHGFGVLGPQGRGALADAGLQHWRLIYVGTLGKA
AGVSGAFAAGHGELVEWLLQKARTYIFTTGAPPALAEALLLSLDLIEAADDRRTHLAGLIERFGAELTLSRWQRLPSRTP
IQPIRIGGNEEALAVARALWDEGLWVPAIRPPTVPAGSARLRVSLTAAHTEADVLRLARTLNRLEHAAHG
>Mature_389_residues
SALLDRLRTDLSALAGQGLRRVRRSNALPCAPRALVDGREMLAFCSNDYLGLAAEPALATALAQASSRWGAGSGASHLVS
GHYAVHDALEHRLAAFVGAERALYFSTGYMANAGIVPALVGRGDAVFADRLNHASLVDGVLLSRADMHRYPHGDLAVLAR
QLAGSSAARKLIVTDAVFSMDGDVAPLADLLELAERHDAWLMVDDAHGFGVLGPQGRGALADAGLQHWRLIYVGTLGKAA
GVSGAFAAGHGELVEWLLQKARTYIFTTGAPPALAEALLLSLDLIEAADDRRTHLAGLIERFGAELTLSRWQRLPSRTPI
QPIRIGGNEEALAVARALWDEGLWVPAIRPPTVPAGSARLRVSLTAAHTEADVLRLARTLNRLEHAAHG

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=378, Percent_Identity=35.7142857142857, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI284448556, Length=355, Percent_Identity=36.3380281690141, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI83977440, Length=349, Percent_Identity=31.8051575931232, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI83977442, Length=349, Percent_Identity=31.8051575931232, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI83977444, Length=349, Percent_Identity=31.8051575931232, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI4758668, Length=346, Percent_Identity=29.4797687861272, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI4502025, Length=361, Percent_Identity=31.0249307479224, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI40316939, Length=361, Percent_Identity=31.0249307479224, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI119220554, Length=363, Percent_Identity=29.4765840220386, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI5454084, Length=394, Percent_Identity=26.3959390862944, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1786993, Length=350, Percent_Identity=45.7142857142857, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1790046, Length=386, Percent_Identity=35.7512953367876, Blast_Score=234, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI71994529, Length=343, Percent_Identity=34.6938775510204, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI32566772, Length=357, Percent_Identity=26.6106442577031, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560914, Length=357, Percent_Identity=26.0504201680672, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560912, Length=357, Percent_Identity=26.0504201680672, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71982625, Length=273, Percent_Identity=24.9084249084249, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71982617, Length=273, Percent_Identity=24.9084249084249, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320438, Length=403, Percent_Identity=28.287841191067, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320267, Length=358, Percent_Identity=26.536312849162, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323954, Length=272, Percent_Identity=27.5735294117647, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24662918, Length=376, Percent_Identity=35.9042553191489, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137420, Length=377, Percent_Identity=31.0344827586207, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI17136286, Length=356, Percent_Identity=26.4044943820225, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24653280, Length=267, Percent_Identity=28.8389513108614, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653276, Length=267, Percent_Identity=28.8389513108614, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653278, Length=267, Percent_Identity=28.8389513108614, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_AZOSB (A1K6Q1)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933393.1
- ProteinModelPortal:   A1K6Q1
- STRING:   A1K6Q1
- GeneID:   4608389
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1889
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   CASAPVV
- PhylomeDB:   A1K6Q1
- ProtClustDB:   CLSK713381
- BioCyc:   ASP62928:AZO1889-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41415; Mature: 41284

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 22-22 BINDING 134-134 BINDING 180-180 BINDING 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLDRLRTDLSALAGQGLRRVRRSNALPCAPRALVDGREMLAFCSNDYLGLAAEPALA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHCCCCCCCCCCHHHH
TALAQASSRWGAGSGASHLVSGHYAVHDALEHRLAAFVGAERALYFSTGYMANAGIVPAL
HHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHH
VGRGDAVFADRLNHASLVDGVLLSRADMHRYPHGDLAVLARQLAGSSAARKLIVTDAVFS
HCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHEEEEEHHHHH
MDGDVAPLADLLELAERHDAWLMVDDAHGFGVLGPQGRGALADAGLQHWRLIYVGTLGKA
CCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHCCHHHEEEEEEECCCCC
AGVSGAFAAGHGELVEWLLQKARTYIFTTGAPPALAEALLLSLDLIEAADDRRTHLAGLI
CCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ERFGAELTLSRWQRLPSRTPIQPIRIGGNEEALAVARALWDEGLWVPAIRPPTVPAGSAR
HHHCCHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
LRVSLTAAHTEADVLRLARTLNRLEHAAHG
EEEEEEECHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SALLDRLRTDLSALAGQGLRRVRRSNALPCAPRALVDGREMLAFCSNDYLGLAAEPALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHCCCCCCCCCCHHHH
TALAQASSRWGAGSGASHLVSGHYAVHDALEHRLAAFVGAERALYFSTGYMANAGIVPAL
HHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHH
VGRGDAVFADRLNHASLVDGVLLSRADMHRYPHGDLAVLARQLAGSSAARKLIVTDAVFS
HCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHEEEEEHHHHH
MDGDVAPLADLLELAERHDAWLMVDDAHGFGVLGPQGRGALADAGLQHWRLIYVGTLGKA
CCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHCCHHHEEEEEEECCCCC
AGVSGAFAAGHGELVEWLLQKARTYIFTTGAPPALAEALLLSLDLIEAADDRRTHLAGLI
CCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ERFGAELTLSRWQRLPSRTPIQPIRIGGNEEALAVARALWDEGLWVPAIRPPTVPAGSAR
HHHCCHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
LRVSLTAAHTEADVLRLARTLNRLEHAAHG
EEEEEEECHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA