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The map label for this gene is bioH [H]

Identifier: 119898181

GI number: 119898181

Start: 2075642

End: 2076406

Strand: Direct

Name: bioH [H]

Synonym: azo1890

Alternate gene names: 119898181

Gene position: 2075642-2076406 (Clockwise)

Preceding gene: 119898180

Following gene: 119898182

Centisome position: 47.43

GC content: 72.81

Gene sequence:

>765_bases
ATGGTTGAGATCGGGATGCCCAGCCTGCCGCCGCTGGTGCTGTTGCACGGCTGGGGCATGACGCCGGCGGTCTGGGACGG
GCTCGCACCGGCGCTGCGCGAGCGCTTCGCCGTATCCGCCCCGCCCCTGCCCTGTCACCCCGGCAGTGAAGTCACGACGG
GAGCGCTGCCCGCATGGGCCGATGCGATCGCCGGCGGCTTGCCGGATGACGCGCTGGTGTGCGGCTGGTCGCTCGGGGCG
CAACTGGCGATGACGCTGGCGCTGCGCCATCCGCACAAGGTAAGACGGTTGCTGCTGGTGGGGGCAACGCCCTGCTTCGT
CGCCGCGCCCGGCTGGGGGGCCGCGCTGGATGCCGCCACGGTGACCGCCTTCGTCGAAGGTTTTCGCGCGTCGCCCGCGC
CGACGCTACGGCGTTTCCTGAGCCTGCAGGTGCAGGGCGAGGCAAGGCGCAAGCCGGTGCTGCAGGCGCTGGGCGCCACC
GCCGTCGCGGCGGATGCGTTGGAGGCGGGAGACGCCGCAGCGCTCGCGGCCGCGCTCGACGTGCTGGTTTCGACCGACCT
GCGCGCGTTGGCTGGCCGCATTACGGCGCCGACCCTGCTGGTCCACGGCGACGGCGACGCCCTGATGCCGCTCGACGCCG
CGCACTGGTTGGCCGAAGCCTTGCCGCACGCGCGCCTGGAGGTTTTCGCCGACAGCGGCCACGCGCCTTTCTTGTCCGAT
CCCGAGCGCTTCGTCGCCCTGCTGGGGGCGCTTGGCGATGCTTGA

Upstream 100 bases:

>100_bases
GCCGGCAGTGCCCGCCTGCGGGTGTCGCTGACCGCTGCCCATACCGAAGCCGACGTGCTTCGTCTCGCGCGGACGCTGAA
CCGCCTGGAGCACGCCGCGC

Downstream 100 bases:

>100_bases
ACCCACGGCACGCAAAGCGGCGGTCAGGCGCGCGTTCGACCGCGCGGCCGACGGTTACGATCGCGCGGCGGACATCCAGC
GCGCAGCCTGCGACCGCTTG

Product: BioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MVEIGMPSLPPLVLLHGWGMTPAVWDGLAPALRERFAVSAPPLPCHPGSEVTTGALPAWADAIAGGLPDDALVCGWSLGA
QLAMTLALRHPHKVRRLLLVGATPCFVAAPGWGAALDAATVTAFVEGFRASPAPTLRRFLSLQVQGEARRKPVLQALGAT
AVAADALEAGDAAALAAALDVLVSTDLRALAGRITAPTLLVHGDGDALMPLDAAHWLAEALPHARLEVFADSGHAPFLSD
PERFVALLGALGDA

Sequences:

>Translated_254_residues
MVEIGMPSLPPLVLLHGWGMTPAVWDGLAPALRERFAVSAPPLPCHPGSEVTTGALPAWADAIAGGLPDDALVCGWSLGA
QLAMTLALRHPHKVRRLLLVGATPCFVAAPGWGAALDAATVTAFVEGFRASPAPTLRRFLSLQVQGEARRKPVLQALGAT
AVAADALEAGDAAALAAALDVLVSTDLRALAGRITAPTLLVHGDGDALMPLDAAHWLAEALPHARLEVFADSGHAPFLSD
PERFVALLGALGDA
>Mature_254_residues
MVEIGMPSLPPLVLLHGWGMTPAVWDGLAPALRERFAVSAPPLPCHPGSEVTTGALPAWADAIAGGLPDDALVCGWSLGA
QLAMTLALRHPHKVRRLLLVGATPCFVAAPGWGAALDAATVTAFVEGFRASPAPTLRRFLSLQVQGEARRKPVLQALGAT
AVAADALEAGDAAALAAALDVLVSTDLRALAGRITAPTLLVHGDGDALMPLDAAHWLAEALPHARLEVFADSGHAPFLSD
PERFVALLGALGDA

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=242, Percent_Identity=33.8842975206612, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 26110; Mature: 26110

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEIGMPSLPPLVLLHGWGMTPAVWDGLAPALRERFAVSAPPLPCHPGSEVTTGALPAWA
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
DAIAGGLPDDALVCGWSLGAQLAMTLALRHPHKVRRLLLVGATPCFVAAPGWGAALDAAT
HHHHCCCCCCCEEECHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEECCCCCHHHHHHH
VTAFVEGFRASPAPTLRRFLSLQVQGEARRKPVLQALGATAVAADALEAGDAAALAAALD
HHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VLVSTDLRALAGRITAPTLLVHGDGDALMPLDAAHWLAEALPHARLEVFADSGHAPFLSD
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
PERFVALLGALGDA
HHHHHHHHHHHCCC
>Mature Secondary Structure
MVEIGMPSLPPLVLLHGWGMTPAVWDGLAPALRERFAVSAPPLPCHPGSEVTTGALPAWA
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
DAIAGGLPDDALVCGWSLGAQLAMTLALRHPHKVRRLLLVGATPCFVAAPGWGAALDAAT
HHHHCCCCCCCEEECHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEECCCCCHHHHHHH
VTAFVEGFRASPAPTLRRFLSLQVQGEARRKPVLQALGATAVAADALEAGDAAALAAALD
HHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VLVSTDLRALAGRITAPTLLVHGDGDALMPLDAAHWLAEALPHARLEVFADSGHAPFLSD
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
PERFVALLGALGDA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA