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The map label for this gene is nuoM [H]

Identifier: 119897699

GI number: 119897699

Start: 1536546

End: 1538027

Strand: Direct

Name: nuoM [H]

Synonym: azo1408

Alternate gene names: 119897699

Gene position: 1536546-1538027 (Clockwise)

Preceding gene: 119897698

Following gene: 119897700

Centisome position: 35.11

GC content: 61.4

Gene sequence:

>1482_bases
ATGACGGACATTCCCTACCTTAGTCTCGCGATCTGGGTGCCGATAATCGGCGGCCTGCTGGTGCTCGCCACCGGCTCCGA
CCGCAATGCTCCGATGGCACGGGTGCTGGCCCTGCTGGCGTCGATCGCGGGCTTCGTCGTAACCCTTCCGCTCTATACCG
GCTTCGACGTCAGTTCGGCTGCGATGCAGTTCGTCGAAATCGCCCCCTGGGTGCCGCGCTTCAACATCAACTACCACCTC
GGCGTCGACGGCATCTCGGTGCTGTTCGTGCTGCTGAATGCCTTCATCACCATCATGGTGGTGATCGCGGGCTGGCAGGT
GATCGAGGAGAAGGTCGCGCAGTACATGGCCGCCTTCCTGATCATGTCCGGGTTGATGAACGGGATCTTCTCCGCACTGG
ACGGCATCCTGTTCTACGTGTTCTTTGAAGCCTCGCTGATCCCGCTCTATCTCGTGATCGGTATCTGGGGCGGCCCGAAC
CGCGTCTATGCCGCAATCAAGTTCTTCCTCTACACGCTGCTCGGCTCGCTGCTGATGCTGATCGCGCTGCTTTACCTGTA
CATGGAAGCCGGCGGGTCGTTCAGCATCCTCGACTGGCACCAGTTGCCCCTCGCAATCGAGCCGCAGACGCTCATCTTCT
TCGCCTTCCTGATCGCGTTCGGGGTGAAGGTGCCGATGTGGCCGGTGCATACCTGGCTGCCCGACGCGCACGTGGAAGCC
CCTACGGGTGGTTCGGTGGTGCTGGCAGCGATCGGCCTGAAGCTGGGGGCCTACGGTTTCCTGCGCTTCTCGCTGCCTAT
CCTGCCGGACGCTGCGCAGCAGCTTGCTCCGGTGGTAATCACGCTGTCGCTGATCGCGGTGGTCTATATCGGTTTCGTCG
CGCTGGTACAGGCCGACATGAAGAAGCTGGTGGCCTACTCGTCGATCTCGCACATGGGTTTCGTGACGCTGGGCTTCTTC
ATGTTCAACCCGCTCGCCGTCGAGGGCGCGCTGGTTCAGATGATCTCCCACGGCTTCGTGTCCGGCGCGATGTTCCTGTG
CATCGGCGTGCTGTACGACCGCATGCACTCGCGCCAGATCGCCGACTACGGTGGTGTTGTGCATTCGATGCCGAAGTTCG
CGGCGTTCTTCATGCTGTTCGCGATGGCGAACGCCGGTCTGCCGGCGACTAGCGGCTTCGTCGGTGAGTTCATGGTGGTG
TTGGGTGCCGTCCAGTACAACTTCTGGATCGCGTTCCTTGCCGCCACCACGCTGATCCTGGGCGCCGCCTACACCTTGTG
GATGTACAAGCGGGTGGTATTCGGGGCGATCGGCAACAAGCACGTCGCCGAACTCGAAGACATCAATGCCCGGGAGTTCG
CCTTCCTCGGCATCCTTGCTGCCTGCGTGTTGGCGATGGGTCTGTATCCCTTCCCCTTTACCGAAGTGATGCATGCCTCG
GTCAATGAACTCCTCCGTCACGTGGCCGTGAGCAAGCTCTGA

Upstream 100 bases:

>100_bases
GACCTTCTGGTTCAACCGCGCCTAAACAGCACGTCGCCCACCAGGCTTTAGACAACAAGATCGAACGCGCCAGGGACGGC
GCATAGAACGGAAAGCAACG

Downstream 100 bases:

>100_bases
AGCCCACAGCGAGACACTTAACCGGACTGGCCAGAAGATGAACTTTGTTGTCCCCGACTTCTACCCCGCGACGGCGGAGA
TCTTCGTCGCCGTGATGGCG

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTDIPYLSLAIWVPIIGGLLVLATGSDRNAPMARVLALLASIAGFVVTLPLYTGFDVSSAAMQFVEIAPWVPRFNINYHL
GVDGISVLFVLLNAFITIMVVIAGWQVIEEKVAQYMAAFLIMSGLMNGIFSALDGILFYVFFEASLIPLYLVIGIWGGPN
RVYAAIKFFLYTLLGSLLMLIALLYLYMEAGGSFSILDWHQLPLAIEPQTLIFFAFLIAFGVKVPMWPVHTWLPDAHVEA
PTGGSVVLAAIGLKLGAYGFLRFSLPILPDAAQQLAPVVITLSLIAVVYIGFVALVQADMKKLVAYSSISHMGFVTLGFF
MFNPLAVEGALVQMISHGFVSGAMFLCIGVLYDRMHSRQIADYGGVVHSMPKFAAFFMLFAMANAGLPATSGFVGEFMVV
LGAVQYNFWIAFLAATTLILGAAYTLWMYKRVVFGAIGNKHVAELEDINAREFAFLGILAACVLAMGLYPFPFTEVMHAS
VNELLRHVAVSKL

Sequences:

>Translated_493_residues
MTDIPYLSLAIWVPIIGGLLVLATGSDRNAPMARVLALLASIAGFVVTLPLYTGFDVSSAAMQFVEIAPWVPRFNINYHL
GVDGISVLFVLLNAFITIMVVIAGWQVIEEKVAQYMAAFLIMSGLMNGIFSALDGILFYVFFEASLIPLYLVIGIWGGPN
RVYAAIKFFLYTLLGSLLMLIALLYLYMEAGGSFSILDWHQLPLAIEPQTLIFFAFLIAFGVKVPMWPVHTWLPDAHVEA
PTGGSVVLAAIGLKLGAYGFLRFSLPILPDAAQQLAPVVITLSLIAVVYIGFVALVQADMKKLVAYSSISHMGFVTLGFF
MFNPLAVEGALVQMISHGFVSGAMFLCIGVLYDRMHSRQIADYGGVVHSMPKFAAFFMLFAMANAGLPATSGFVGEFMVV
LGAVQYNFWIAFLAATTLILGAAYTLWMYKRVVFGAIGNKHVAELEDINAREFAFLGILAACVLAMGLYPFPFTEVMHAS
VNELLRHVAVSKL
>Mature_492_residues
TDIPYLSLAIWVPIIGGLLVLATGSDRNAPMARVLALLASIAGFVVTLPLYTGFDVSSAAMQFVEIAPWVPRFNINYHLG
VDGISVLFVLLNAFITIMVVIAGWQVIEEKVAQYMAAFLIMSGLMNGIFSALDGILFYVFFEASLIPLYLVIGIWGGPNR
VYAAIKFFLYTLLGSLLMLIALLYLYMEAGGSFSILDWHQLPLAIEPQTLIFFAFLIAFGVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIGLKLGAYGFLRFSLPILPDAAQQLAPVVITLSLIAVVYIGFVALVQADMKKLVAYSSISHMGFVTLGFFM
FNPLAVEGALVQMISHGFVSGAMFLCIGVLYDRMHSRQIADYGGVVHSMPKFAAFFMLFAMANAGLPATSGFVGEFMVVL
GAVQYNFWIAFLAATTLILGAAYTLWMYKRVVFGAIGNKHVAELEDINAREFAFLGILAACVLAMGLYPFPFTEVMHASV
NELLRHVAVSKL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=348, Percent_Identity=35.632183908046, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI251831117, Length=386, Percent_Identity=23.8341968911917, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788613, Length=500, Percent_Identity=35.6, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788827, Length=274, Percent_Identity=28.1021897810219, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788831, Length=282, Percent_Identity=27.6595744680851, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788829, Length=414, Percent_Identity=26.8115942028986, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788614, Length=413, Percent_Identity=26.1501210653753, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693160, Length=349, Percent_Identity=25.214899713467, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI2367154, Length=246, Percent_Identity=26.4227642276423, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53670; Mature: 53539

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIPYLSLAIWVPIIGGLLVLATGSDRNAPMARVLALLASIAGFVVTLPLYTGFDVSSA
CCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AMQFVEIAPWVPRFNINYHLGVDGISVLFVLLNAFITIMVVIAGWQVIEEKVAQYMAAFL
HHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMSGLMNGIFSALDGILFYVFFEASLIPLYLVIGIWGGPNRVYAAIKFFLYTLLGSLLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IALLYLYMEAGGSFSILDWHQLPLAIEPQTLIFFAFLIAFGVKVPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCEEEEECCCCCCEECCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
PTGGSVVLAAIGLKLGAYGFLRFSLPILPDAAQQLAPVVITLSLIAVVYIGFVALVQADM
CCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLVAYSSISHMGFVTLGFFMFNPLAVEGALVQMISHGFVSGAMFLCIGVLYDRMHSRQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADYGGVVHSMPKFAAFFMLFAMANAGLPATSGFVGEFMVVLGAVQYNFWIAFLAATTLIL
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAYTLWMYKRVVFGAIGNKHVAELEDINAREFAFLGILAACVLAMGLYPFPFTEVMHAS
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VNELLRHVAVSKL
HHHHHHHHHHHCC
>Mature Secondary Structure 
TDIPYLSLAIWVPIIGGLLVLATGSDRNAPMARVLALLASIAGFVVTLPLYTGFDVSSA
CCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AMQFVEIAPWVPRFNINYHLGVDGISVLFVLLNAFITIMVVIAGWQVIEEKVAQYMAAFL
HHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMSGLMNGIFSALDGILFYVFFEASLIPLYLVIGIWGGPNRVYAAIKFFLYTLLGSLLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IALLYLYMEAGGSFSILDWHQLPLAIEPQTLIFFAFLIAFGVKVPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCEEEEECCCCCCEECCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
PTGGSVVLAAIGLKLGAYGFLRFSLPILPDAAQQLAPVVITLSLIAVVYIGFVALVQADM
CCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLVAYSSISHMGFVTLGFFMFNPLAVEGALVQMISHGFVSGAMFLCIGVLYDRMHSRQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADYGGVVHSMPKFAAFFMLFAMANAGLPATSGFVGEFMVVLGAVQYNFWIAFLAATTLIL
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAYTLWMYKRVVFGAIGNKHVAELEDINAREFAFLGILAACVLAMGLYPFPFTEVMHAS
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VNELLRHVAVSKL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]