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The map label for this gene is nuoN

Identifier: 119897700

GI number: 119897700

Start: 1538065

End: 1539540

Strand: Direct

Name: nuoN

Synonym: azo1409

Alternate gene names: 119897700

Gene position: 1538065-1539540 (Clockwise)

Preceding gene: 119897699

Following gene: 119897701

Centisome position: 35.15

GC content: 61.86

Gene sequence:

>1476_bases
ATGAACTTTGTTGTCCCCGACTTCTACCCCGCGACGGCGGAGATCTTCGTCGCCGTGATGGCGCTCGTGATCATGCTGGC
GACGACCTTCGCGCGCAGCGTGGCGCGGGGTCTCGCCTACGGTCTCACGCTGGTGACGCTGATCGGCGCCGCGATCATCA
CCTACAACACCGCTAATCCGCGGGCGGTCACGACCTTCAGCAACATGTTCATCGGCGACCTGCTCGGCGATGTGCTGAAG
CTGCTGATCTACTTCTCGATGGCGGTGGCACTGCTCTACGGCCGCAGCTATCTGGCGGATCGCAAGCTCGACAAGCCTGA
GTACTACGTCCTGGCGCTGCTGATGACGCTGGGCATGATGGTCATGGTGACCTCCAACCACCTGCTGTCGATGTACATCG
GTCTGGAGCTGATGTCGCTGTCGCTGTACGCGCTGGTCGCATTCGACCGCGATTCGGCGCGCGGCACCGAAGCGGCAATG
AAGTACTTCGTGCTCGGTGCGCTCGCTTCGGGTCTGCTGCTTTACGGCATGTCGATGCTCTACGGCGCGACCGGCACGCT
GGAGATTTCGGGCATTGCCAAGTCGGTGTACAACCAGGCCGCCAATGACACGGTGCTGCTGTTCGGTCTTGTCTTCCTGA
TGGCGGGCATCTGCTTCAAGCTGGGCGTCGTGCCCTTCCACATGTGGATCCCCGATGTCTATCACGGAGCCAACACCGCG
GTCACGCTGATCATCGCAACCGCGCCCAAGCTGGCTGCGTTCGCGATGGCGGTGCGCCTGCTGGTCTGGGGCCTGTTCGA
TGTCGCCGAGCACTGGCAGACGATGCTGATGTTCGTTGCGGTGCTGTCCATCGTGCTGGGCAACCTCGCCGCGATCGCGC
AGACCAATCTGAAGCGGATGCTGGCCTACTCGGGCATCTCGCACATGGGTTTCATGCTGCTCGGACTCCTGTCCGGCGTG
GTGGATGGTGATCCGCACTACGCGCTCGACGCCTACAGCGCGGCGATGTTCTACGCCATCTCCTACGTGATCATGAGCCT
CGCGTCCTTCGGCATGATCATCCTGTTGTCGCGCGCTGGCTTCGAAGCCGAGAACATCGACGACTTCAAGGGGCTGAACA
AGCGCAGTCCGTGGTTTGCCGCGATGATGATGTTCGTGATGTTCTCGATGGCCGGCATCCCGTTCTTCATCGGTTTCTTT
GCCAAGCTTGCGGTACTGCAGGCGGTCGTGGCGGCGGGCTACATCTGGGTGGCGGTGGTCGCGGTACTGATGTCGGTGAT
CGGCGCGTTCTACTACCTGCGTCTGGTCAAAGTCATGTACTTCGATGAACCGGCCGATGCAACGCCGATTCAGGCGCCGG
CCGAACTGCGGGTGTTCCTGTCCGCGAATGGCCTGGCGATTGCCGCGATCGGGCTCGCACCGCAAGGTGTGATGACGCTC
TGCACCTTCGTGCTGCTGGCATCCACCCAGCCCTGA

Upstream 100 bases:

>100_bases
ACCGAAGTGATGCATGCCTCGGTCAATGAACTCCTCCGTCACGTGGCCGTGAGCAAGCTCTGAAGCCCACAGCGAGACAC
TTAACCGGACTGGCCAGAAG

Downstream 100 bases:

>100_bases
CCCGGCACACCTTACCCGACGACGCCGACGCGGATCATTCCGTCGTCGGCGTTGTCGTTTCCGGTTCCTACATTCACGAG
GTTGTTGATGAGCGCGCAAA

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNFVVPDFYPATAEIFVAVMALVIMLATTFARSVARGLAYGLTLVTLIGAAIITYNTANPRAVTTFSNMFIGDLLGDVLK
LLIYFSMAVALLYGRSYLADRKLDKPEYYVLALLMTLGMMVMVTSNHLLSMYIGLELMSLSLYALVAFDRDSARGTEAAM
KYFVLGALASGLLLYGMSMLYGATGTLEISGIAKSVYNQAANDTVLLFGLVFLMAGICFKLGVVPFHMWIPDVYHGANTA
VTLIIATAPKLAAFAMAVRLLVWGLFDVAEHWQTMLMFVAVLSIVLGNLAAIAQTNLKRMLAYSGISHMGFMLLGLLSGV
VDGDPHYALDAYSAAMFYAISYVIMSLASFGMIILLSRAGFEAENIDDFKGLNKRSPWFAAMMMFVMFSMAGIPFFIGFF
AKLAVLQAVVAAGYIWVAVVAVLMSVIGAFYYLRLVKVMYFDEPADATPIQAPAELRVFLSANGLAIAAIGLAPQGVMTL
CTFVLLASTQP

Sequences:

>Translated_491_residues
MNFVVPDFYPATAEIFVAVMALVIMLATTFARSVARGLAYGLTLVTLIGAAIITYNTANPRAVTTFSNMFIGDLLGDVLK
LLIYFSMAVALLYGRSYLADRKLDKPEYYVLALLMTLGMMVMVTSNHLLSMYIGLELMSLSLYALVAFDRDSARGTEAAM
KYFVLGALASGLLLYGMSMLYGATGTLEISGIAKSVYNQAANDTVLLFGLVFLMAGICFKLGVVPFHMWIPDVYHGANTA
VTLIIATAPKLAAFAMAVRLLVWGLFDVAEHWQTMLMFVAVLSIVLGNLAAIAQTNLKRMLAYSGISHMGFMLLGLLSGV
VDGDPHYALDAYSAAMFYAISYVIMSLASFGMIILLSRAGFEAENIDDFKGLNKRSPWFAAMMMFVMFSMAGIPFFIGFF
AKLAVLQAVVAAGYIWVAVVAVLMSVIGAFYYLRLVKVMYFDEPADATPIQAPAELRVFLSANGLAIAAIGLAPQGVMTL
CTFVLLASTQP
>Mature_491_residues
MNFVVPDFYPATAEIFVAVMALVIMLATTFARSVARGLAYGLTLVTLIGAAIITYNTANPRAVTTFSNMFIGDLLGDVLK
LLIYFSMAVALLYGRSYLADRKLDKPEYYVLALLMTLGMMVMVTSNHLLSMYIGLELMSLSLYALVAFDRDSARGTEAAM
KYFVLGALASGLLLYGMSMLYGATGTLEISGIAKSVYNQAANDTVLLFGLVFLMAGICFKLGVVPFHMWIPDVYHGANTA
VTLIIATAPKLAAFAMAVRLLVWGLFDVAEHWQTMLMFVAVLSIVLGNLAAIAQTNLKRMLAYSGISHMGFMLLGLLSGV
VDGDPHYALDAYSAAMFYAISYVIMSLASFGMIILLSRAGFEAENIDDFKGLNKRSPWFAAMMMFVMFSMAGIPFFIGFF
AKLAVLQAVVAAGYIWVAVVAVLMSVIGAFYYLRLVKVMYFDEPADATPIQAPAELRVFLSANGLAIAAIGLAPQGVMTL
CTFVLLASTQP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=321, Percent_Identity=24.6105919003115, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI145693160, Length=394, Percent_Identity=35.2791878172589, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1788614, Length=331, Percent_Identity=26.5861027190332, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788827, Length=290, Percent_Identity=24.1379310344828, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788831, Length=334, Percent_Identity=23.9520958083832, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788613, Length=273, Percent_Identity=26.7399267399267, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_AZOSB (A1K5C1)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932913.1
- STRING:   A1K5C1
- GeneID:   4609431
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1409
- HOGENOM:   HBG747830
- OMA:   FAGWADH
- PhylomeDB:   A1K5C1
- ProtClustDB:   PRK05777
- BioCyc:   ASP62928:AZO1409-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 53129; Mature: 53129

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ba98650)-; HASH(0x1b6055c8)-; HASH(0x1ba8aad4)-; HASH(0x1b6c8f1c)-; HASH(0x1ba7d3ec)-; HASH(0x1b0f3fd4)-; HASH(0x1afa4b74)-; HASH(0x1b6b7004)-; HASH(0x1a92cec8)-; HASH(0x19d0b530)-; HASH(0x1a7c4d54)-; HASH(0x1b575ff8)-; HASH(0x1a755650)-; HASH(0x1b383dbc)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVVPDFYPATAEIFVAVMALVIMLATTFARSVARGLAYGLTLVTLIGAAIITYNTANP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
RAVTTFSNMFIGDLLGDVLKLLIYFSMAVALLYGRSYLADRKLDKPEYYVLALLMTLGMM
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VMVTSNHLLSMYIGLELMSLSLYALVAFDRDSARGTEAAMKYFVLGALASGLLLYGMSML
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGATGTLEISGIAKSVYNQAANDTVLLFGLVFLMAGICFKLGVVPFHMWIPDVYHGANTA
HCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCE
VTLIIATAPKLAAFAMAVRLLVWGLFDVAEHWQTMLMFVAVLSIVLGNLAAIAQTNLKRM
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYSGISHMGFMLLGLLSGVVDGDPHYALDAYSAAMFYAISYVIMSLASFGMIILLSRAG
HHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FEAENIDDFKGLNKRSPWFAAMMMFVMFSMAGIPFFIGFFAKLAVLQAVVAAGYIWVAVV
CCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLMSVIGAFYYLRLVKVMYFDEPADATPIQAPAELRVFLSANGLAIAAIGLAPQGVMTL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCCEEEEECCCCCCHHHH
CTFVLLASTQP
HHHHHHHCCCH
>Mature Secondary Structure
MNFVVPDFYPATAEIFVAVMALVIMLATTFARSVARGLAYGLTLVTLIGAAIITYNTANP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
RAVTTFSNMFIGDLLGDVLKLLIYFSMAVALLYGRSYLADRKLDKPEYYVLALLMTLGMM
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VMVTSNHLLSMYIGLELMSLSLYALVAFDRDSARGTEAAMKYFVLGALASGLLLYGMSML
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGATGTLEISGIAKSVYNQAANDTVLLFGLVFLMAGICFKLGVVPFHMWIPDVYHGANTA
HCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCE
VTLIIATAPKLAAFAMAVRLLVWGLFDVAEHWQTMLMFVAVLSIVLGNLAAIAQTNLKRM
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYSGISHMGFMLLGLLSGVVDGDPHYALDAYSAAMFYAISYVIMSLASFGMIILLSRAG
HHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FEAENIDDFKGLNKRSPWFAAMMMFVMFSMAGIPFFIGFFAKLAVLQAVVAAGYIWVAVV
CCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLMSVIGAFYYLRLVKVMYFDEPADATPIQAPAELRVFLSANGLAIAAIGLAPQGVMTL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCCEEEEECCCCCCHHHH
CTFVLLASTQP
HHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA