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The map label for this gene is purK [H]

Identifier: 119897670

GI number: 119897670

Start: 1506220

End: 1507401

Strand: Direct

Name: purK [H]

Synonym: azo1379

Alternate gene names: 119897670

Gene position: 1506220-1507401 (Clockwise)

Preceding gene: 119897669

Following gene: 119897671

Centisome position: 34.42

GC content: 68.61

Gene sequence:

>1182_bases
ATGTCCGAATCCGCCCTGTCCGCGCCCATCCTGCCGCCCGCCACGCTGGGCATGTTGGGGGGAGGCCAGCTCGGCCGCTT
CTTCGTCTCCGCGGCACACGAGATGGGGTACAAGGTCTGGGTGCTCGACCCTGACCCCCACAGTCCCGCCGGGCGTATCG
CCGATTTCCATCTCGTCGCCGCCTATGACGACTATGCCGCGCTCGACCGCGTGGCGGCGGAGTGCGCCGCGGTGACCACC
GAGTTCGAGAACGTGCCCGCCGCGACGCTGGACTATCTGGCGAAGTTCGTCCCGGTGCGACCCGGGGCAGCCGCCGTCGC
GGTGTGTCAGAACCGCATCGAGGAAAAGGGTTTTCTGGCCGGCAACGGATTGCCGCACGGCCCATATGCCGCCATCCGCA
GCGATGAGGATTTGCGCGCGGCCGACGCTGCGCTCTTTCCTGCAGTGCTTAAAGTGGCGCGCTTCGGCTACGACGGCAAG
GGGCAGGCGCGGGTCGCCGACGTGGATGAGGCCTTGGCGGCCTTTCATCAGTTCAAGGGCGAGCCCTGCGTGCTGGAAAA
ACTGCTCCGGCTCGACTTCGAACTTTCGGTCGTGCTGGCGCGCGACGAGGCGGGCGCGGTGCGGTGTTTCCCGGCCGCCG
AGAATCGTCACCGCCGCGGCATCCTGGATGTGACGATTGCGCCCGCGCAATCGGCCCCGGCCGGCCTGCGCGACGAAGCG
CAGGTGGTCGCGGCGCGAATCGCGGAGCACCTCGGCTATGTTGGAACGCTGGGGGTGGAGTTCTTTGTCTGCGAGGGCGT
CCTCTACGTCAATGAGATGGCGCCGCGGCCGCACAACAGCGGCCATCACACCATCGACGCCTGCGACGTCAGCCAGTACG
ACCAACAGGTGCGCGCGCTGTGCGGGCTGCCGCTGGGCGTGCCCCGTCAGCACTCCGCCGCGGCGATGGTGAATCTGCTG
GGCGAGCTGTGGTACGCCGACGGGCACGCCACGGGTGCCTACCGTGAACCGGACTGGCGAGTGCTCGCCGACGTGCCCGG
CCTGAGGCTCCATCTGTACGGTAAGCACCACGCCCGCCCGGGGCGCAAGATGGGGCATTTCACGGTGGTGGGCGAGGATC
AGGCGGCCGTGCTGGCGCAGGCCTTGCGTGCTCGCGGCGCGATCGGGGTGACTGACGAATGA

Upstream 100 bases:

>100_bases
CCGGGGAAGGGCACGCCGGATGTCATCGCCCCGACGTGCTTCCGGCGCCGTCCCTCTTCCGTCCATCTCCCTTTCGCCGA
CCGATTGTTCCGAGGCTCGC

Downstream 100 bases:

>100_bases
GGCCGCTCGGCGTCGTGCCGGCGCAGGAAGACAACATCCAGAGTGCTGCGGAGGCGCTGCGCGCAGGCGAACTTGTCGGC
CTGCCGACCGAAACCGTGTA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSESALSAPILPPATLGMLGGGQLGRFFVSAAHEMGYKVWVLDPDPHSPAGRIADFHLVAAYDDYAALDRVAAECAAVTT
EFENVPAATLDYLAKFVPVRPGAAAVAVCQNRIEEKGFLAGNGLPHGPYAAIRSDEDLRAADAALFPAVLKVARFGYDGK
GQARVADVDEALAAFHQFKGEPCVLEKLLRLDFELSVVLARDEAGAVRCFPAAENRHRRGILDVTIAPAQSAPAGLRDEA
QVVAARIAEHLGYVGTLGVEFFVCEGVLYVNEMAPRPHNSGHHTIDACDVSQYDQQVRALCGLPLGVPRQHSAAAMVNLL
GELWYADGHATGAYREPDWRVLADVPGLRLHLYGKHHARPGRKMGHFTVVGEDQAAVLAQALRARGAIGVTDE

Sequences:

>Translated_393_residues
MSESALSAPILPPATLGMLGGGQLGRFFVSAAHEMGYKVWVLDPDPHSPAGRIADFHLVAAYDDYAALDRVAAECAAVTT
EFENVPAATLDYLAKFVPVRPGAAAVAVCQNRIEEKGFLAGNGLPHGPYAAIRSDEDLRAADAALFPAVLKVARFGYDGK
GQARVADVDEALAAFHQFKGEPCVLEKLLRLDFELSVVLARDEAGAVRCFPAAENRHRRGILDVTIAPAQSAPAGLRDEA
QVVAARIAEHLGYVGTLGVEFFVCEGVLYVNEMAPRPHNSGHHTIDACDVSQYDQQVRALCGLPLGVPRQHSAAAMVNLL
GELWYADGHATGAYREPDWRVLADVPGLRLHLYGKHHARPGRKMGHFTVVGEDQAAVLAQALRARGAIGVTDE
>Mature_392_residues
SESALSAPILPPATLGMLGGGQLGRFFVSAAHEMGYKVWVLDPDPHSPAGRIADFHLVAAYDDYAALDRVAAECAAVTTE
FENVPAATLDYLAKFVPVRPGAAAVAVCQNRIEEKGFLAGNGLPHGPYAAIRSDEDLRAADAALFPAVLKVARFGYDGKG
QARVADVDEALAAFHQFKGEPCVLEKLLRLDFELSVVLARDEAGAVRCFPAAENRHRRGILDVTIAPAQSAPAGLRDEAQ
VVAARIAEHLGYVGTLGVEFFVCEGVLYVNEMAPRPHNSGHHTIDACDVSQYDQQVRALCGLPLGVPRQHSAAAMVNLLG
ELWYADGHATGAYREPDWRVLADVPGLRLHLYGKHHARPGRKMGHFTVVGEDQAAVLAQALRARGAIGVTDE

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=364, Percent_Identity=30.2197802197802, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1788155, Length=399, Percent_Identity=27.3182957393484, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324702, Length=382, Percent_Identity=33.2460732984293, Blast_Score=190, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42047; Mature: 41916

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESALSAPILPPATLGMLGGGQLGRFFVSAAHEMGYKVWVLDPDPHSPAGRIADFHLVA
CCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEE
AYDDYAALDRVAAECAAVTTEFENVPAATLDYLAKFVPVRPGAAAVAVCQNRIEEKGFLA
ECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
GNGLPHGPYAAIRSDEDLRAADAALFPAVLKVARFGYDGKGQARVADVDEALAAFHQFKG
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCC
EPCVLEKLLRLDFELSVVLARDEAGAVRCFPAAENRHRRGILDVTIAPAQSAPAGLRDEA
CHHHHHHHHHCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCHHH
QVVAARIAEHLGYVGTLGVEFFVCEGVLYVNEMAPRPHNSGHHTIDACDVSQYDQQVRAL
HHHHHHHHHHHCCHHHHHHEEEECCCEEEEHHCCCCCCCCCCCEEEECCHHHHHHHHHHH
CGLPLGVPRQHSAAAMVNLLGELWYADGHATGAYREPDWRVLADVPGLRLHLYGKHHARP
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCC
GRKMGHFTVVGEDQAAVLAQALRARGAIGVTDE
CCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SESALSAPILPPATLGMLGGGQLGRFFVSAAHEMGYKVWVLDPDPHSPAGRIADFHLVA
CCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEE
AYDDYAALDRVAAECAAVTTEFENVPAATLDYLAKFVPVRPGAAAVAVCQNRIEEKGFLA
ECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
GNGLPHGPYAAIRSDEDLRAADAALFPAVLKVARFGYDGKGQARVADVDEALAAFHQFKG
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCC
EPCVLEKLLRLDFELSVVLARDEAGAVRCFPAAENRHRRGILDVTIAPAQSAPAGLRDEA
CHHHHHHHHHCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCHHH
QVVAARIAEHLGYVGTLGVEFFVCEGVLYVNEMAPRPHNSGHHTIDACDVSQYDQQVRAL
HHHHHHHHHHHCCHHHHHHEEEECCCEEEEHHCCCCCCCCCCCEEEECCHHHHHHHHHHH
CGLPLGVPRQHSAAAMVNLLGELWYADGHATGAYREPDWRVLADVPGLRLHLYGKHHARP
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCC
GRKMGHFTVVGEDQAAVLAQALRARGAIGVTDE
CCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]