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The map label for this gene is leuD

Identifier: 119897331

GI number: 119897331

Start: 1118505

End: 1119143

Strand: Direct

Name: leuD

Synonym: azo1040

Alternate gene names: 119897331

Gene position: 1118505-1119143 (Clockwise)

Preceding gene: 119897330

Following gene: 119897332

Centisome position: 25.56

GC content: 63.07

Gene sequence:

>639_bases
ATGAAACCGTTTACCGTTCTCGATGCGATCGTGGCGCCGCTTGACCGTGCCAACGTCGATACCGATGCGATCATTCCCAA
GCAGTTCCTGAAGTCGATCAAGCGCAGCGGCTTCGGCCCCAACCTGTTCGACGAATGGCGTTACCTTGACGTCGGCCAGC
CCGGCCAGGACTGCAGCAACCGCCCGAAGAACCCGGACTTCGTGCTCAACCAGGCGCGCTATCAGGGGGCGCAGGTACTG
CTCGCACGCGACAACTTCGGCTGCGGCAGCTCGCGCGAGCACGCGCCGTGGGCGCTGGAAGACTACGGCTTCCGCGTGAT
CATCGCGCCGAGCTTCGCCGACATCTTCTTCAACAACAGCTTCAAGAACGGCCTGCTGCCGATCAAGCTGGATGCCGCCG
AACTCGACGTGCTGTTCCAGCAGTGCGAGGCCACCGAAGGCTACCGCCTGAAGGTGGACCTTGCGGCGCAGACGATCACC
CGCCCGGACGGCAAGGCGATCGCCTTCGATGTCGATCCCTTCCGCAAGGAATGCCTGTTGAACGGTTGGGACGACATCGG
CCTGACCCTGCGCCATGCGGACAAGATCCGCGACTTCGAAGCGAAGCGCCGCGCCGAGCACCCCTATTACTTCGCCTGA

Upstream 100 bases:

>100_bases
GGGCGCACCCATCTGGTGAGCCCGGCGATGGCTGCGGCCGCGGCCGTCACCGGACATTTCACCGACGTGCGCACCCTGAA
CTGACACGACAGGCAGAACC

Downstream 100 bases:

>100_bases
GCCGCTGCGGCCCGGGTTTGTGTGGAGAATCACTGGATGAAGATTTGCGTGTTGCCGGGCGACGGCATCGGTCCCGAAAT
CACGGCAGAAGCCGTGCGCG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKPFTVLDAIVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDVGQPGQDCSNRPKNPDFVLNQARYQGAQVL
LARDNFGCGSSREHAPWALEDYGFRVIIAPSFADIFFNNSFKNGLLPIKLDAAELDVLFQQCEATEGYRLKVDLAAQTIT
RPDGKAIAFDVDPFRKECLLNGWDDIGLTLRHADKIRDFEAKRRAEHPYYFA

Sequences:

>Translated_212_residues
MKPFTVLDAIVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDVGQPGQDCSNRPKNPDFVLNQARYQGAQVL
LARDNFGCGSSREHAPWALEDYGFRVIIAPSFADIFFNNSFKNGLLPIKLDAAELDVLFQQCEATEGYRLKVDLAAQTIT
RPDGKAIAFDVDPFRKECLLNGWDDIGLTLRHADKIRDFEAKRRAEHPYYFA
>Mature_212_residues
MKPFTVLDAIVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDVGQPGQDCSNRPKNPDFVLNQARYQGAQVL
LARDNFGCGSSREHAPWALEDYGFRVIIAPSFADIFFNNSFKNGLLPIKLDAAELDVLFQQCEATEGYRLKVDLAAQTIT
RPDGKAIAFDVDPFRKECLLNGWDDIGLTLRHADKIRDFEAKRRAEHPYYFA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=58.8832487309645, Blast_Score=233, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6321429, Length=213, Percent_Identity=53.0516431924883, Blast_Score=214, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_AZOSB (A1K4A2)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932544.1
- ProteinModelPortal:   A1K4A2
- SMR:   A1K4A2
- STRING:   A1K4A2
- GeneID:   4609132
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1040
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   A1K4A2
- ProtClustDB:   PRK01641
- BioCyc:   ASP62928:AZO1040-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24016; Mature: 24016

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFTVLDAIVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDVGQPGQDCSN
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCHHC
RPKNPDFVLNQARYQGAQVLLARDNFGCGSSREHAPWALEDYGFRVIIAPSFADIFFNNS
CCCCCCCEEEHHHCCCEEEEEEECCCCCCCCCCCCCEEECCCCEEEEECCCCEEHEECCC
FKNGLLPIKLDAAELDVLFQQCEATEGYRLKVDLAAQTITRPDGKAIAFDVDPFRKECLL
CCCCEEEEEECHHHHHHHHHHHCCCCCEEEEEEEEEHEECCCCCCEEEEECCHHHHHHHH
NGWDDIGLTLRHADKIRDFEAKRRAEHPYYFA
CCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPFTVLDAIVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDVGQPGQDCSN
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCHHC
RPKNPDFVLNQARYQGAQVLLARDNFGCGSSREHAPWALEDYGFRVIIAPSFADIFFNNS
CCCCCCCEEEHHHCCCEEEEEEECCCCCCCCCCCCCEEECCCCEEEEECCCCEEHEECCC
FKNGLLPIKLDAAELDVLFQQCEATEGYRLKVDLAAQTITRPDGKAIAFDVDPFRKECLL
CCCCEEEEEECHHHHHHHHHHHCCCCCEEEEEEEEEHEECCCCCCEEEEECCHHHHHHHH
NGWDDIGLTLRHADKIRDFEAKRRAEHPYYFA
CCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA