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The map label for this gene is leuC

Identifier: 119897330

GI number: 119897330

Start: 1117079

End: 1118488

Strand: Direct

Name: leuC

Synonym: azo1039

Alternate gene names: 119897330

Gene position: 1117079-1118488 (Clockwise)

Preceding gene: 119897329

Following gene: 119897331

Centisome position: 25.53

GC content: 66.88

Gene sequence:

>1410_bases
ATGGAAGCCCAAACGCTGTACGAAAAGCTCTGGTCGAGTCATGTCGTTCACCAGGAGGCCGACGGCACGGCGCTGATCTA
CATCGATCGCCACCTCGTCCACGAAGTCACCAGCCCGCAGGCCTTCGAAGGGCTGAAGCTGGCCGGCCGCAAGCCGTGGC
GCATCTCGTCCATCGTCGCCACCGCCGACCACAACATCCCCACTGACCACTGGGAGATGGGTATCCAGGACCCGGTGTCG
CGCCAGCAGGTCGAAACCCTGGACGCCAACATCCGTGAAGTGGGCTCGCTGGCCTATTTCCCGTTCAAGGACCAGCGGCA
GGGCATCATCCATGTGATCGGGCCGGAAAACGGCACCACGCTGCCGGGCATGACCGTGGTCTGTGGTGACTCGCACACTT
CGACGCACGGCGCCTTCGCCTGTCTGGCGCACGGCATCGGCACCTCCGAGGTCGAGCACGTGATGGCGACCCAGTGCCTG
CTGCAGAAGAAGTCCAAGACCATGCTGATCAAGGTCGAAGGCACGCTCGGCCGCGGCGTCACCGCCAAGGACGTCGTGCT
CGCCATCATCGGCCGGATCGGCACCGCCGGCGGTACCGGCTACGCCATCGAGTTCGGCGGCAGCGCGATCCGCTCGCTGT
CCATGGAAGGCCGCATGACGGTCTGCAACATGGCGATCGAGGCCGGCGCGCGCGCCGGTCTGGTGGCGGTGGACGAAACC
ACCATCGACTACCTCAAGGACAAGCCTTTCGCGCCCAAGGGGCCGCAGTGGGATGCCGCGGTGGCGTACTGGCGCACGTT
GAAGTCCGACGACGGCGCCACCTTCGACACCGTGGTGGAACTGGATGCCACCTCGATCCTGCCGCAGGTCACCTGGGGCA
CCTCGCCCGAGATGGTGACCACGGTGGACGGGCGGGTGCCGGATCCGGCCGCGATCGCCGATCCGGTCAAGCGCGAAGGC
GTCGAGCGCGCGCTCAAGTACATGGGGCTGGCGCCGAATACGCCGATCAGCGAGATCGCGGTCGACCAGGTCTTCATCGG
TTCGTGCACCAACTCGCGCATCGAGGACCTGCGCGAGGCCGCCGCGGTCGTCAAGGGACGCAGCAAGGCCGCTTCTGTGC
GCCGCGTGCTGGTGGTGCCGGGGTCCGGTCTGGTCAAGCACCAGGCGGAAGCCGAGGGTCTGGACAAGGTGTTCATCGAG
GCGGGTTTCGAATGGCGGGAGCCGGGCTGTTCGATGTGTCTGGCGATGAACGCCGACCGCCTCGAGCCGGGCGAGCGTTG
CGCGTCCACCTCCAACCGCAATTTCGAAGGCCGTCAGGGCGCTGGCGGGCGCACCCATCTGGTGAGCCCGGCGATGGCTG
CGGCCGCGGCCGTCACCGGACATTTCACCGACGTGCGCACCCTGAACTGA

Upstream 100 bases:

>100_bases
CGCTAGGCGACGGACGGCCTGCAAGGCCGTCCGGCGGCTGTTCAGCGGCGCAGCCTTTGTGAAATAATCGGAACCTTTTC
TTTCCCCGGAAGCGCACTGG

Downstream 100 bases:

>100_bases
CACGACAGGCAGAACCATGAAACCGTTTACCGTTCTCGATGCGATCGTGGCGCCGCTTGACCGTGCCAACGTCGATACCG
ATGCGATCATTCCCAAGCAG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MEAQTLYEKLWSSHVVHQEADGTALIYIDRHLVHEVTSPQAFEGLKLAGRKPWRISSIVATADHNIPTDHWEMGIQDPVS
RQQVETLDANIREVGSLAYFPFKDQRQGIIHVIGPENGTTLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVMATQCL
LQKKSKTMLIKVEGTLGRGVTAKDVVLAIIGRIGTAGGTGYAIEFGGSAIRSLSMEGRMTVCNMAIEAGARAGLVAVDET
TIDYLKDKPFAPKGPQWDAAVAYWRTLKSDDGATFDTVVELDATSILPQVTWGTSPEMVTTVDGRVPDPAAIADPVKREG
VERALKYMGLAPNTPISEIAVDQVFIGSCTNSRIEDLREAAAVVKGRSKAASVRRVLVVPGSGLVKHQAEAEGLDKVFIE
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVTGHFTDVRTLN

Sequences:

>Translated_469_residues
MEAQTLYEKLWSSHVVHQEADGTALIYIDRHLVHEVTSPQAFEGLKLAGRKPWRISSIVATADHNIPTDHWEMGIQDPVS
RQQVETLDANIREVGSLAYFPFKDQRQGIIHVIGPENGTTLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVMATQCL
LQKKSKTMLIKVEGTLGRGVTAKDVVLAIIGRIGTAGGTGYAIEFGGSAIRSLSMEGRMTVCNMAIEAGARAGLVAVDET
TIDYLKDKPFAPKGPQWDAAVAYWRTLKSDDGATFDTVVELDATSILPQVTWGTSPEMVTTVDGRVPDPAAIADPVKREG
VERALKYMGLAPNTPISEIAVDQVFIGSCTNSRIEDLREAAAVVKGRSKAASVRRVLVVPGSGLVKHQAEAEGLDKVFIE
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVTGHFTDVRTLN
>Mature_469_residues
MEAQTLYEKLWSSHVVHQEADGTALIYIDRHLVHEVTSPQAFEGLKLAGRKPWRISSIVATADHNIPTDHWEMGIQDPVS
RQQVETLDANIREVGSLAYFPFKDQRQGIIHVIGPENGTTLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVMATQCL
LQKKSKTMLIKVEGTLGRGVTAKDVVLAIIGRIGTAGGTGYAIEFGGSAIRSLSMEGRMTVCNMAIEAGARAGLVAVDET
TIDYLKDKPFAPKGPQWDAAVAYWRTLKSDDGATFDTVVELDATSILPQVTWGTSPEMVTTVDGRVPDPAAIADPVKREG
VERALKYMGLAPNTPISEIAVDQVFIGSCTNSRIEDLREAAAVVKGRSKAASVRRVLVVPGSGLVKHQAEAEGLDKVFIE
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVTGHFTDVRTLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=27.8236914600551, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI8659555, Length=402, Percent_Identity=26.6169154228856, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI41352693, Length=367, Percent_Identity=26.9754768392371, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=64.8068669527897, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=25.414364640884, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=24.0896358543417, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI2367097, Length=392, Percent_Identity=25.2551020408163, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=29.8630136986301, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=29.8630136986301, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=319, Percent_Identity=28.8401253918495, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=469, Percent_Identity=25.7995735607676, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=62.8450106157113, Blast_Score=610, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=29.9168975069252, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=362, Percent_Identity=29.2817679558011, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=466, Percent_Identity=25.3218884120172, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI281365315, Length=475, Percent_Identity=26.3157894736842, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17864292, Length=475, Percent_Identity=26.3157894736842, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161076999, Length=362, Percent_Identity=27.3480662983425, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=28.6501377410468, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645686, Length=376, Percent_Identity=27.9255319148936, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137564, Length=372, Percent_Identity=27.1505376344086, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_AZOSB (A1K4A1)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932543.1
- ProteinModelPortal:   A1K4A1
- SMR:   A1K4A1
- STRING:   A1K4A1
- GeneID:   4606673
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1039
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   A1K4A1
- ProtClustDB:   PRK05478
- BioCyc:   ASP62928:AZO1039-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50255; Mature: 50255

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAQTLYEKLWSSHVVHQEADGTALIYIDRHLVHEVTSPQAFEGLKLAGRKPWRISSIVA
CCHHHHHHHHHHHHCEEECCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCCEEEEEEE
TADHNIPTDHWEMGIQDPVSRQQVETLDANIREVGSLAYFPFKDQRQGIIHVIGPENGTT
ECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVMATQCLLQKKSKTMLIKVEGTLGRGV
CCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
TAKDVVLAIIGRIGTAGGTGYAIEFGGSAIRSLSMEGRMTVCNMAIEAGARAGLVAVDET
CHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCEEHHHHHCCCCCCEEEECCH
TIDYLKDKPFAPKGPQWDAAVAYWRTLKSDDGATFDTVVELDATSILPQVTWGTSPEMVT
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCCCCCEEE
TVDGRVPDPAAIADPVKREGVERALKYMGLAPNTPISEIAVDQVFIGSCTNSRIEDLREA
EECCCCCCCHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCHHHHHHHHH
AAVVKGRSKAASVRRVLVVPGSGLVKHQAEAEGLDKVFIEAGFEWREPGCSMCLAMNADR
HHHHHCCCHHCCEEEEEEECCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVTGHFTDVRTLN
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MEAQTLYEKLWSSHVVHQEADGTALIYIDRHLVHEVTSPQAFEGLKLAGRKPWRISSIVA
CCHHHHHHHHHHHHCEEECCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCCEEEEEEE
TADHNIPTDHWEMGIQDPVSRQQVETLDANIREVGSLAYFPFKDQRQGIIHVIGPENGTT
ECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCCCC
LPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVMATQCLLQKKSKTMLIKVEGTLGRGV
CCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
TAKDVVLAIIGRIGTAGGTGYAIEFGGSAIRSLSMEGRMTVCNMAIEAGARAGLVAVDET
CHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCEEHHHHHCCCCCCEEEECCH
TIDYLKDKPFAPKGPQWDAAVAYWRTLKSDDGATFDTVVELDATSILPQVTWGTSPEMVT
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCCCCCEEE
TVDGRVPDPAAIADPVKREGVERALKYMGLAPNTPISEIAVDQVFIGSCTNSRIEDLREA
EECCCCCCCHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCHHHHHHHHH
AAVVKGRSKAASVRRVLVVPGSGLVKHQAEAEGLDKVFIEAGFEWREPGCSMCLAMNADR
HHHHHCCCHHCCEEEEEEECCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVTGHFTDVRTLN
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA