Click here to switch to the map view.

The map label for this gene is leuB [H]

Identifier: 119897332

GI number: 119897332

Start: 1119180

End: 1120244

Strand: Direct

Name: leuB [H]

Synonym: azo1041

Alternate gene names: 119897332

Gene position: 1119180-1120244 (Clockwise)

Preceding gene: 119897331

Following gene: 119897333

Centisome position: 25.58

GC content: 65.73

Gene sequence:

>1065_bases
ATGAAGATTTGCGTGTTGCCGGGCGACGGCATCGGTCCCGAAATCACGGCAGAAGCCGTGCGCGTGCTGCGGGCGCTCGA
TCTGAAGATCGAACTGGAAGAAGCGCTGCTCGGCGGCAGCGCGGTGGATGCCACCGGCACGCCCTACCCGGAGGCGACGC
AGAAGCTTGCGCGCGCCGCCGACGCCGTGCTGCTCGGCGCGGTCGGCGGCCCGAAGTGGGACACGCTGCCGCGCGAACAG
CGCCCCGAGCGCGGCCTGCTCGGCATCCGCAAGGATCTCAGCCTCTTCGCCAACCTGCGTCCGGCCATCCTGTATCCGGA
ACTGGCCAACGCCTCCACGCTGAAGCCGGAGGTGGTGTCGGGGCTCGACATCCTGATCGTCCGCGAACTCACCGGCGACA
TCTACTTCGGCCAGCCGCGCGGCATCGAAACCCGCGAGGTCAATGGCGCCCAGCAGCGCGTGGGCTGGAACACGATGATC
TACGCCGAGTACGAGATCCGCCGCATCCTCAAGGTGGCGTTCGAAGCGGCGCAGAAGCGCGGCAAGAAGCTGTGCTCGGT
CGACAAGATGAACGTGCTCGAAACCACTCAACTCTGGCGCGATATCGCCGATGAAATGGCGAAGGACTTCCCGGATGTGG
AGCTGTCGCACATGCTGGTCGACAACGCCGCGATGCAGCTGGTGCGCAATCCCAAGCAGTTCGACGTGATGGTCACCGGC
AACATGTTCGGCGACATCCTGTCGGACGAGGCCTCGATGCTCACCGGCTCGATCGGGATGCTGCCGTCGGCCTCGCTCGA
CGCCAACAACAAGGGGCTGTACGAGCCTTCGCACGGCTCGGCGCCGGACATCGCCGGCAAGGGCGTGGCCAACCCGCTGG
CCACCATCCTGTCGGCGGCGATGATGCTGCGTTATACGTTCAATCAGGAAGAAGCCGCGGTGCGCATCGAGCGCGCGGTC
AAGAAGGTGCTGGCGCAGGGTTTCCGCACCGGCGACATCTACGAGGCAGGTACGAAGAAGGTCGGCACCAGGGAAATGGG
CGACGCAGTGCTGGCGGCGCTCTGA

Upstream 100 bases:

>100_bases
TGCGGACAAGATCCGCGACTTCGAAGCGAAGCGCCGCGCCGAGCACCCCTATTACTTCGCCTGAGCCGCTGCGGCCCGGG
TTTGTGTGGAGAATCACTGG

Downstream 100 bases:

>100_bases
GCGAGCGTTGCCGGGCGGCATGTAAGGTTTGTTGATTCGAGGTGATGTGATGAAGCGAGTAGGTCTGGTTGGCTGGCGCG
GCATGGTCGGTTCCGTGCTG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH 1; Beta-IPM dehydrogenase 1; IMDH 1 [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKICVLPGDGIGPEITAEAVRVLRALDLKIELEEALLGGSAVDATGTPYPEATQKLARAADAVLLGAVGGPKWDTLPREQ
RPERGLLGIRKDLSLFANLRPAILYPELANASTLKPEVVSGLDILIVRELTGDIYFGQPRGIETREVNGAQQRVGWNTMI
YAEYEIRRILKVAFEAAQKRGKKLCSVDKMNVLETTQLWRDIADEMAKDFPDVELSHMLVDNAAMQLVRNPKQFDVMVTG
NMFGDILSDEASMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYTFNQEEAAVRIERAV
KKVLAQGFRTGDIYEAGTKKVGTREMGDAVLAAL

Sequences:

>Translated_354_residues
MKICVLPGDGIGPEITAEAVRVLRALDLKIELEEALLGGSAVDATGTPYPEATQKLARAADAVLLGAVGGPKWDTLPREQ
RPERGLLGIRKDLSLFANLRPAILYPELANASTLKPEVVSGLDILIVRELTGDIYFGQPRGIETREVNGAQQRVGWNTMI
YAEYEIRRILKVAFEAAQKRGKKLCSVDKMNVLETTQLWRDIADEMAKDFPDVELSHMLVDNAAMQLVRNPKQFDVMVTG
NMFGDILSDEASMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYTFNQEEAAVRIERAV
KKVLAQGFRTGDIYEAGTKKVGTREMGDAVLAAL
>Mature_354_residues
MKICVLPGDGIGPEITAEAVRVLRALDLKIELEEALLGGSAVDATGTPYPEATQKLARAADAVLLGAVGGPKWDTLPREQ
RPERGLLGIRKDLSLFANLRPAILYPELANASTLKPEVVSGLDILIVRELTGDIYFGQPRGIETREVNGAQQRVGWNTMI
YAEYEIRRILKVAFEAAQKRGKKLCSVDKMNVLETTQLWRDIADEMAKDFPDVELSHMLVDNAAMQLVRNPKQFDVMVTG
NMFGDILSDEASMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYTFNQEEAAVRIERAV
KKVLAQGFRTGDIYEAGTKKVGTREMGDAVLAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=330, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI28178816, Length=357, Percent_Identity=26.890756302521, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI28178821, Length=308, Percent_Identity=27.9220779220779, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4758582, Length=339, Percent_Identity=29.7935103244838, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178838, Length=320, Percent_Identity=30, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=36.1702127659575, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=52.9577464788732, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1788101, Length=364, Percent_Identity=34.0659340659341, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=24.8743718592965, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=343, Percent_Identity=34.402332361516, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=30.7262569832402, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17505779, Length=333, Percent_Identity=29.7297297297297, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=31.4606741573034, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=48.6263736263736, Blast_Score=304, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6322097, Length=371, Percent_Identity=34.7708894878706, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=359, Percent_Identity=33.1476323119777, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=333, Percent_Identity=30.9309309309309, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24643268, Length=338, Percent_Identity=34.9112426035503, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24643270, Length=338, Percent_Identity=34.9112426035503, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24661184, Length=366, Percent_Identity=31.1475409836066, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078635, Length=339, Percent_Identity=28.023598820059, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=28.023598820059, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=28.023598820059, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=28.023598820059, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=28.1899109792285, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=30.0578034682081, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=30.0578034682081, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20130355, Length=338, Percent_Identity=26.3313609467456, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38382; Mature: 38382

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKICVLPGDGIGPEITAEAVRVLRALDLKIELEEALLGGSAVDATGTPYPEATQKLARAA
CEEEEECCCCCCCHHHHHHHHHHHHHCCEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
DAVLLGAVGGPKWDTLPREQRPERGLLGIRKDLSLFANLRPAILYPELANASTLKPEVVS
HHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHC
GLDILIVRELTGDIYFGQPRGIETREVNGAQQRVGWNTMIYAEYEIRRILKVAFEAAQKR
CCCEEEEEECCCCEEECCCCCCCHHCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
GKKLCSVDKMNVLETTQLWRDIADEMAKDFPDVELSHMLVDNAAMQLVRNPKQFDVMVTG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
NMFGDILSDEASMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAA
CHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MMLRYTFNQEEAAVRIERAVKKVLAQGFRTGDIYEAGTKKVGTREMGDAVLAAL
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKICVLPGDGIGPEITAEAVRVLRALDLKIELEEALLGGSAVDATGTPYPEATQKLARAA
CEEEEECCCCCCCHHHHHHHHHHHHHCCEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
DAVLLGAVGGPKWDTLPREQRPERGLLGIRKDLSLFANLRPAILYPELANASTLKPEVVS
HHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHC
GLDILIVRELTGDIYFGQPRGIETREVNGAQQRVGWNTMIYAEYEIRRILKVAFEAAQKR
CCCEEEEEECCCCEEECCCCCCCHHCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
GKKLCSVDKMNVLETTQLWRDIADEMAKDFPDVELSHMLVDNAAMQLVRNPKQFDVMVTG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
NMFGDILSDEASMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAA
CHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MMLRYTFNQEEAAVRIERAVKKVLAQGFRTGDIYEAGTKKVGTREMGDAVLAAL
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA