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The map label for this gene is nifB [H]

Identifier: 119896814

GI number: 119896814

Start: 559180

End: 560676

Strand: Direct

Name: nifB [H]

Synonym: azo0523

Alternate gene names: 119896814

Gene position: 559180-560676 (Clockwise)

Preceding gene: 119896810

Following gene: 119896815

Centisome position: 12.78

GC content: 65.13

Gene sequence:

>1497_bases
ATGGAACTCCCGGTTATCAGCAATACCGCGCCCGAGTCCTCGGGCGGCGGCTGTAGCTCAAGCGGCTGTGGGACCGCGCC
CGACGCGCTCTCCCACCTGCCGGACAGCATCCGCAGCAAGGTTCAGGACCACCCCTGTTATTCGGAAGAAGCCCACCACT
ACTTCGCGCGCATGCACGTCGCGGTGGCGCCGGCCTGCAACATCCAGTGCAACTACTGCAACCGCAAGTACGACTGCTCC
AACGAATCGCGCCCGGGCGTCGTGTCCGAAGTGATGAGCCCGGACCAGGCGGTCAAGAAGACGCTCGCGGTCGCCGCGGC
GATTCCGCAGATGACGGTGCTCGGCATCGCCGGACCCGGAGACCCCCTCGCCAACCCCGAGCGCACCTTCGAGACCTTCC
GCCGTCTCTCCGAAGAAGCGCCGGACATCAAGCTGTGCGTGTCCACCAACGGCCTGTCGCTGCCGGAAAGCGTGGATGAA
CTGGCCAAGCACAACATCGACCACGTCACCATCACGATCAACTGTGTGGACCCGGAGATCGGCGCCAAGATCTACCCGTG
GATCTTCTGGGAAAACAAACGCATCTTCGGCGTCGAAGGCGCGAAGATCCTCATCGAGCAGCAGCAGAAGGGCCTCGAGA
TGCTGACCTCGCGCGGCATCCTGGTGAAGGTCAACTCGGTGATGATCCCCGGCATCAACGACGAGCACCTCAAGGAGGTC
TCGAAGATCGTCAAGGCCAAGGGCGCGTTCCTGCACAACGTGATGCCGCTGATCGCGGAGGCCGAGCACGGCACCTACTT
CGGCATCATGGGCCAGCGCGAACCGACGCCGCTGGAACTCGAAGCGCTGCAGGACTCCTGCTCGGGCGACATGTCGATGA
TGCGCCACTGCCGCCAGTGCCGTGCCGATGCGGTCGGCCTGCTGGGCGAGGACCGCGGCGCGGAGTTCACCATCGACAAG
ATCGAAGAGATGGAGATCGACTACGCCGCCGCCATGGTCGAGCGCGCCAAGGTGCACGACGCGATCACCTCCGAGTTGGA
AATGAAGCGCGCGCTCAAGGCCGCGCCCAAGTTCAACCCGGAAGCCGGTGAAGAGAAGCCCGCCACGCGCCCGATCCGCA
TCGCGATCGCCAGCAAGGGCGGCGGCCTGGTGAACGAGCACTTCGGCCACGCGCACGAGTTCCTGGTGTACGAGGTCGGC
CCCACCAGCCACCGCTTCCTCACCCACCGCAAGTGCACGCCCTACTGCATGGGCGACGAATCCTGCGGTGACGGCGAGAC
GGTGCTCGGCCAGATCATCAAGAGCCTGGAAGGCGTCGAAGTGCTGCTCGCCAGCAAGATCGGCTTCGAGCCCTGGGCCG
ATCTGGAAAAGGCCGGCATCCAGCCCAACGGCGAGCACGCGATGGAGCCGATCGAAGAGGCCATCCGCGCCGTGTATGAC
GAGCTGGCCGCCGCCGGCAAGCTTGACATGCCGGCCGAGGCCAAGGCTGCCGCTTGA

Upstream 100 bases:

>100_bases
AGAATAAGTCCTGTGTTTTCAATGGCCTGATTCCCTGCTTGCGGCTGGCACGGGGCTTGCGAAAGTCATCCGCGTAAGAC
TTCGCTAAAGGGGATCGACA

Downstream 100 bases:

>100_bases
TGGTGGCAGGGGAGCCCGCGCTGGGGCGCGGGCTCCCGTTTTCCGCCCCTTGAAGGTTCCGCATCACCGTTCCGCACCCA
ATTTCCGCCTGCACCCAACG

Product: nitrogen fixation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MELPVISNTAPESSGGGCSSSGCGTAPDALSHLPDSIRSKVQDHPCYSEEAHHYFARMHVAVAPACNIQCNYCNRKYDCS
NESRPGVVSEVMSPDQAVKKTLAVAAAIPQMTVLGIAGPGDPLANPERTFETFRRLSEEAPDIKLCVSTNGLSLPESVDE
LAKHNIDHVTITINCVDPEIGAKIYPWIFWENKRIFGVEGAKILIEQQQKGLEMLTSRGILVKVNSVMIPGINDEHLKEV
SKIVKAKGAFLHNVMPLIAEAEHGTYFGIMGQREPTPLELEALQDSCSGDMSMMRHCRQCRADAVGLLGEDRGAEFTIDK
IEEMEIDYAAAMVERAKVHDAITSELEMKRALKAAPKFNPEAGEEKPATRPIRIAIASKGGGLVNEHFGHAHEFLVYEVG
PTSHRFLTHRKCTPYCMGDESCGDGETVLGQIIKSLEGVEVLLASKIGFEPWADLEKAGIQPNGEHAMEPIEEAIRAVYD
ELAAAGKLDMPAEAKAAA

Sequences:

>Translated_498_residues
MELPVISNTAPESSGGGCSSSGCGTAPDALSHLPDSIRSKVQDHPCYSEEAHHYFARMHVAVAPACNIQCNYCNRKYDCS
NESRPGVVSEVMSPDQAVKKTLAVAAAIPQMTVLGIAGPGDPLANPERTFETFRRLSEEAPDIKLCVSTNGLSLPESVDE
LAKHNIDHVTITINCVDPEIGAKIYPWIFWENKRIFGVEGAKILIEQQQKGLEMLTSRGILVKVNSVMIPGINDEHLKEV
SKIVKAKGAFLHNVMPLIAEAEHGTYFGIMGQREPTPLELEALQDSCSGDMSMMRHCRQCRADAVGLLGEDRGAEFTIDK
IEEMEIDYAAAMVERAKVHDAITSELEMKRALKAAPKFNPEAGEEKPATRPIRIAIASKGGGLVNEHFGHAHEFLVYEVG
PTSHRFLTHRKCTPYCMGDESCGDGETVLGQIIKSLEGVEVLLASKIGFEPWADLEKAGIQPNGEHAMEPIEEAIRAVYD
ELAAAGKLDMPAEAKAAA
>Mature_498_residues
MELPVISNTAPESSGGGCSSSGCGTAPDALSHLPDSIRSKVQDHPCYSEEAHHYFARMHVAVAPACNIQCNYCNRKYDCS
NESRPGVVSEVMSPDQAVKKTLAVAAAIPQMTVLGIAGPGDPLANPERTFETFRRLSEEAPDIKLCVSTNGLSLPESVDE
LAKHNIDHVTITINCVDPEIGAKIYPWIFWENKRIFGVEGAKILIEQQQKGLEMLTSRGILVKVNSVMIPGINDEHLKEV
SKIVKAKGAFLHNVMPLIAEAEHGTYFGIMGQREPTPLELEALQDSCSGDMSMMRHCRQCRADAVGLLGEDRGAEFTIDK
IEEMEIDYAAAMVERAKVHDAITSELEMKRALKAAPKFNPEAGEEKPATRPIRIAIASKGGGLVNEHFGHAHEFLVYEVG
PTSHRFLTHRKCTPYCMGDESCGDGETVLGQIIKSLEGVEVLLASKIGFEPWADLEKAGIQPNGEHAMEPIEEAIRAVYD
ELAAAGKLDMPAEAKAAA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 54094; Mature: 54094

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPVISNTAPESSGGGCSSSGCGTAPDALSHLPDSIRSKVQDHPCYSEEAHHYFARMHV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHH
AVAPACNIQCNYCNRKYDCSNESRPGVVSEVMSPDQAVKKTLAVAAAIPQMTVLGIAGPG
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCC
DPLANPERTFETFRRLSEEAPDIKLCVSTNGLSLPESVDELAKHNIDHVTITINCVDPEI
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEECCCC
GAKIYPWIFWENKRIFGVEGAKILIEQQQKGLEMLTSRGILVKVNSVMIPGINDEHLKEV
CCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHH
SKIVKAKGAFLHNVMPLIAEAEHGTYFGIMGQREPTPLELEALQDSCSGDMSMMRHCRQC
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
RADAVGLLGEDRGAEFTIDKIEEMEIDYAAAMVERAKVHDAITSELEMKRALKAAPKFNP
HHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EAGEEKPATRPIRIAIASKGGGLVNEHFGHAHEFLVYEVGPTSHRFLTHRKCTPYCMGDE
CCCCCCCCCCCEEEEEECCCCCCHHHHCCCCEEEEEEEECCCCCHHEECCCCCCCCCCCC
SCGDGETVLGQIIKSLEGVEVLLASKIGFEPWADLEKAGIQPNGEHAMEPIEEAIRAVYD
CCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHH
ELAAAGKLDMPAEAKAAA
HHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MELPVISNTAPESSGGGCSSSGCGTAPDALSHLPDSIRSKVQDHPCYSEEAHHYFARMHV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHH
AVAPACNIQCNYCNRKYDCSNESRPGVVSEVMSPDQAVKKTLAVAAAIPQMTVLGIAGPG
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCC
DPLANPERTFETFRRLSEEAPDIKLCVSTNGLSLPESVDELAKHNIDHVTITINCVDPEI
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEECCCC
GAKIYPWIFWENKRIFGVEGAKILIEQQQKGLEMLTSRGILVKVNSVMIPGINDEHLKEV
CCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHH
SKIVKAKGAFLHNVMPLIAEAEHGTYFGIMGQREPTPLELEALQDSCSGDMSMMRHCRQC
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
RADAVGLLGEDRGAEFTIDKIEEMEIDYAAAMVERAKVHDAITSELEMKRALKAAPKFNP
HHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EAGEEKPATRPIRIAIASKGGGLVNEHFGHAHEFLVYEVGPTSHRFLTHRKCTPYCMGDE
CCCCCCCCCCCEEEEEECCCCCCHHHHCCCCEEEEEEEECCCCCHHEECCCCCCCCCCCC
SCGDGETVLGQIIKSLEGVEVLLASKIGFEPWADLEKAGIQPNGEHAMEPIEEAIRAVYD
CCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHH
ELAAAGKLDMPAEAKAAA
HHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2450865 [H]