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The map label for this gene is nifA [H]

Identifier: 119896810

GI number: 119896810

Start: 554604

End: 556157

Strand: Direct

Name: nifA [H]

Synonym: azo0519

Alternate gene names: 119896810

Gene position: 554604-556157 (Clockwise)

Preceding gene: 119896809

Following gene: 119896814

Centisome position: 12.67

GC content: 69.43

Gene sequence:

>1554_bases
ATGAGCGCGGCCGGTCCGATGCCCGCCGCCGAGGAGCGCGTCGCCTGGCTGGAGGCCACGCTGGAGGCGCTGTACCGCGT
CAGCCGCGTGCTGTCGCGCTCGCTCGAGCTGCGCGAAACCCTGTCCGAAGTGCTGCGCGTGCTCGACGAGGAGTGCGGCT
TCAACCGCGCGCTGGTCACGCTCAACGAGCCCGACGGCGAATCCATGGCGATTTCCGCGCTACATGGCGTCGATGCGCCG
ATCGATCCTGACATCCGCTGGCGTTCCGGCGAAGGCGTGATCGGCTCCACCCAGCAGCGTTCGCGCCCGCTGGTGGTCGC
GCGCCTCGCGGACGACCCCAACTTCCTGTCGCGGCGCGGCCTGTTCGACGGCGAGGCGCCCTTCATCGGCGCGCCGATCC
GCGCCGGGCACGAAACCGTGGGCGTGCTCGCGCTGCAGCCCTCGCCCACCAGCCGCGAGCACCTGGACGACTACGCGCAT
TTCGCCGAGATGGTGGCCAACCTGGTCGGGCAGACGGTGCGGCTGTCCTGTCAGGTGCGGCAGGAACGCCGCGACATCGC
CGAGGAACGCGACAACCTGCGCCGCACCGTGCGCAACCGCTTCGGCTTCGACAACATCGTCGGCCACACCCAGCGCATGC
GCCAGGTGTTCGAGCAGATCCGCCAGGTGGCGAAGTGGAACACCACGGTGCTGGTGCGCGGCGAAACCGGCACCGGCAAG
GAACTCATCGCGCAGGCGATCCACTACAACTCGCCGCGCGCGGCCGGCCCCTTCGTCAAGCTCAACTGTGCGGCGCTGCC
CGAGAACCTCCTCGAGTCCGAACTCTTCGGCCACGAAAAGGGCGCCTTCACCGGCGCGCTGACGCAGCGCAAGGGCCGCT
TCGAGATGGCCGACGGCGGCACGCTGTTCCTCGATGAAATCGGCGAAATCTCGGCATCGTTCCAGGCCAAGCTGCTGCGC
GTGCTGCAGGAGGGCGAACTGGAACGCGTCGGCGGCGCGCGCACGCTGCGGGTGGATGTGCGGGTGATCGCGGCGACCAA
CCGCGACCTCGAACTGGAGGTCGAAGCGGGCAAGTTCCGCGAGGACCTGTACTACCGCCTCAACGTGATGCCGATCATGC
TGCCGCCGCTGCGCGAGCGCGTGGAGGACATCCCGGAAATCGCCCGCTTCCTGGTCGAGAAGGTGTCGGGCCAGCAGGGC
CGGCCGCTGTCGATCACCGACAGCGCGCTGCGCATCCTGCTGCACCACGCCTGGCCGGGCAATGTGCGCGAACTCGAGAA
CTGCATCGAGCGCGCGGCGGTGATGAGCGAGGACGGCACCATCGACCGCGACCTGATCCTGATCTCCGGCATCGAGGAGC
GGGTGACGCCGCTGCGCGGTGGCGGCGGCACGGTGGATCTGGACGACCCGGGGCTGGACGAGCGCGAGCGGGTGATCGCG
GCGCTGGAGCAGGCCGGCTGGGTGCAGGCCAAGGCGGCGCGCCTGCTGGGCATGACCCCGCGCCAGATCGCTTATCGCAT
CCAGACGCTCAACATCAAGGTGCGGCAGATCTGA

Upstream 100 bases:

>100_bases
GCGGCATGCTCGAGATCGACCCTGAGTACAGCGGCGGCTGCCGCCTGCGCATCAGCTTTCCGGCGCTGCGCTCGGCCCCG
CTCGACCTGGAGGGCGGTCG

Downstream 100 bases:

>100_bases
TCCGCCGCGCACCGCGCCGGGCGGGCCGGCGCGGTGCCGCCCGGTCAGCGCAGCGTGGCCTCGCTGCGCGCCAGGCTGGG
CGCGGCCGTCGGCACCAGCT

Product: nif-specific regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSAAGPMPAAEERVAWLEATLEALYRVSRVLSRSLELRETLSEVLRVLDEECGFNRALVTLNEPDGESMAISALHGVDAP
IDPDIRWRSGEGVIGSTQQRSRPLVVARLADDPNFLSRRGLFDGEAPFIGAPIRAGHETVGVLALQPSPTSREHLDDYAH
FAEMVANLVGQTVRLSCQVRQERRDIAEERDNLRRTVRNRFGFDNIVGHTQRMRQVFEQIRQVAKWNTTVLVRGETGTGK
ELIAQAIHYNSPRAAGPFVKLNCAALPENLLESELFGHEKGAFTGALTQRKGRFEMADGGTLFLDEIGEISASFQAKLLR
VLQEGELERVGGARTLRVDVRVIAATNRDLELEVEAGKFREDLYYRLNVMPIMLPPLRERVEDIPEIARFLVEKVSGQQG
RPLSITDSALRILLHHAWPGNVRELENCIERAAVMSEDGTIDRDLILISGIEERVTPLRGGGGTVDLDDPGLDERERVIA
ALEQAGWVQAKAARLLGMTPRQIAYRIQTLNIKVRQI

Sequences:

>Translated_517_residues
MSAAGPMPAAEERVAWLEATLEALYRVSRVLSRSLELRETLSEVLRVLDEECGFNRALVTLNEPDGESMAISALHGVDAP
IDPDIRWRSGEGVIGSTQQRSRPLVVARLADDPNFLSRRGLFDGEAPFIGAPIRAGHETVGVLALQPSPTSREHLDDYAH
FAEMVANLVGQTVRLSCQVRQERRDIAEERDNLRRTVRNRFGFDNIVGHTQRMRQVFEQIRQVAKWNTTVLVRGETGTGK
ELIAQAIHYNSPRAAGPFVKLNCAALPENLLESELFGHEKGAFTGALTQRKGRFEMADGGTLFLDEIGEISASFQAKLLR
VLQEGELERVGGARTLRVDVRVIAATNRDLELEVEAGKFREDLYYRLNVMPIMLPPLRERVEDIPEIARFLVEKVSGQQG
RPLSITDSALRILLHHAWPGNVRELENCIERAAVMSEDGTIDRDLILISGIEERVTPLRGGGGTVDLDDPGLDERERVIA
ALEQAGWVQAKAARLLGMTPRQIAYRIQTLNIKVRQI
>Mature_516_residues
SAAGPMPAAEERVAWLEATLEALYRVSRVLSRSLELRETLSEVLRVLDEECGFNRALVTLNEPDGESMAISALHGVDAPI
DPDIRWRSGEGVIGSTQQRSRPLVVARLADDPNFLSRRGLFDGEAPFIGAPIRAGHETVGVLALQPSPTSREHLDDYAHF
AEMVANLVGQTVRLSCQVRQERRDIAEERDNLRRTVRNRFGFDNIVGHTQRMRQVFEQIRQVAKWNTTVLVRGETGTGKE
LIAQAIHYNSPRAAGPFVKLNCAALPENLLESELFGHEKGAFTGALTQRKGRFEMADGGTLFLDEIGEISASFQAKLLRV
LQEGELERVGGARTLRVDVRVIAATNRDLELEVEAGKFREDLYYRLNVMPIMLPPLRERVEDIPEIARFLVEKVSGQQGR
PLSITDSALRILLHHAWPGNVRELENCIERAAVMSEDGTIDRDLILISGIEERVTPLRGGGGTVDLDDPGLDERERVIAA
LEQAGWVQAKAARLLGMTPRQIAYRIQTLNIKVRQI

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=480, Percent_Identity=40.4166666666667, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1788550, Length=338, Percent_Identity=45.8579881656805, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=48.8745980707396, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1789087, Length=502, Percent_Identity=38.6454183266932, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=57.5107296137339, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1790299, Length=236, Percent_Identity=53.8135593220339, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1788905, Length=243, Percent_Identity=48.559670781893, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI87081872, Length=313, Percent_Identity=45.0479233226837, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1786524, Length=296, Percent_Identity=43.2432432432432, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1789233, Length=285, Percent_Identity=43.859649122807, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1787583, Length=232, Percent_Identity=47.4137931034483, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1789828, Length=248, Percent_Identity=40.7258064516129, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI87081858, Length=221, Percent_Identity=39.3665158371041, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 57574; Mature: 57442

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAGPMPAAEERVAWLEATLEALYRVSRVLSRSLELRETLSEVLRVLDEECGFNRALVT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LNEPDGESMAISALHGVDAPIDPDIRWRSGEGVIGSTQQRSRPLVVARLADDPNFLSRRG
EECCCCCCEEEHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHCC
LFDGEAPFIGAPIRAGHETVGVLALQPSPTSREHLDDYAHFAEMVANLVGQTVRLSCQVR
CCCCCCCEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHEEHHHHH
QERRDIAEERDNLRRTVRNRFGFDNIVGHTQRMRQVFEQIRQVAKWNTTVLVRGETGTGK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
ELIAQAIHYNSPRAAGPFVKLNCAALPENLLESELFGHEKGAFTGALTQRKGRFEMADGG
HHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCHHHHHCCCCCEEECCCC
TLFLDEIGEISASFQAKLLRVLQEGELERVGGARTLRVDVRVIAATNRDLELEVEAGKFR
EEEHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEEEEEECCCCEEEEECCCHHH
EDLYYRLNVMPIMLPPLRERVEDIPEIARFLVEKVSGQQGRPLSITDSALRILLHHAWPG
HHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
NVRELENCIERAAVMSEDGTIDRDLILISGIEERVTPLRGGGGTVDLDDPGLDERERVIA
CHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHCCCCCCCCCEEECCCCCCCHHHHHHH
ALEQAGWVQAKAARLLGMTPRQIAYRIQTLNIKVRQI
HHHHCCCHHHHHHHHHCCCHHHHHEEEEEEEEEEECC
>Mature Secondary Structure 
SAAGPMPAAEERVAWLEATLEALYRVSRVLSRSLELRETLSEVLRVLDEECGFNRALVT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LNEPDGESMAISALHGVDAPIDPDIRWRSGEGVIGSTQQRSRPLVVARLADDPNFLSRRG
EECCCCCCEEEHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHCC
LFDGEAPFIGAPIRAGHETVGVLALQPSPTSREHLDDYAHFAEMVANLVGQTVRLSCQVR
CCCCCCCEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHEEHHHHH
QERRDIAEERDNLRRTVRNRFGFDNIVGHTQRMRQVFEQIRQVAKWNTTVLVRGETGTGK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
ELIAQAIHYNSPRAAGPFVKLNCAALPENLLESELFGHEKGAFTGALTQRKGRFEMADGG
HHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCHHHHHCCCCCEEECCCC
TLFLDEIGEISASFQAKLLRVLQEGELERVGGARTLRVDVRVIAATNRDLELEVEAGKFR
EEEHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEEEEEECCCCEEEEECCCHHH
EDLYYRLNVMPIMLPPLRERVEDIPEIARFLVEKVSGQQGRPLSITDSALRILLHHAWPG
HHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
NVRELENCIERAAVMSEDGTIDRDLILISGIEERVTPLRGGGGTVDLDDPGLDERERVIA
CHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHCCCCCCCCCEEECCCCCCCHHHHHHH
ALEQAGWVQAKAARLLGMTPRQIAYRIQTLNIKVRQI
HHHHCCCHHHHHHHHHCCCHHHHHEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840552; 2450865 [H]