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The map label for this gene is ragB [H]

Identifier: 119896749

GI number: 119896749

Start: 488874

End: 490262

Strand: Direct

Name: ragB [H]

Synonym: azo0458

Alternate gene names: 119896749

Gene position: 488874-490262 (Clockwise)

Preceding gene: 119896748

Following gene: 119896750

Centisome position: 11.17

GC content: 72.64

Gene sequence:

>1389_bases
ATGCCGCGTGAGCGCGCCGGCGGGCTGCGAGCCTGGTTCGCCAGCCCCTTCCACCGCTTCGCGGTCGCGCTGCCGGCGGC
CATCGCGCTGATCGTCGGTGCGCTGTTCTACCCGCTGTTCATCGAGGCCGAGGCCCACATCCGCGAGGAGGTGCAGGCCG
CGATCGAGTTCGAGATCGCCGGGCTGGAGGCGCATTTCCACGAACGCGGCCTCGCCGGCCTGCGCGACGCGCTGCAGCGG
CGCATCGAGATTTCGCCCGACCGTGCCGCGGTCTACCTGCTCGCCGACCGCGACGGCCGCCCGCTGGCGGGCAACCTGGC
GCAATGGCCGGCGGGGGTGTCCGCCAACGACGAGGCGTGGTTTCACGTCGCCGAAGCGGACGGCGAGACGCTGGAAGGGC
GGGTCTTCATCCTGTTCGGCGGCGAACGCCTGCTGGTCGGGCGGCGCTCGCCGCTAGAGGCGTTCCAGCGCAACATGACC
GTGCGGCTGTGGTGGTCGGCGGCGCTGATCATCCTTGTCGCCGGCGTCATCGGCGGCCGCTTCATGCAGCACCTGCACCG
CCGCCTGGCGCGGCTGGCGGCCGAGGCGGGCCGCATCCAGGAAGGCCACCTCGCGCAGCGCCTGTCGCTGAGCGCGCGCG
GCGACGAGCTGGATGCGCTCGCCGAACGCTTCAACAGCGCCTTCGACGAGATCGAGCGTCTGGTGGACGCCGCCAAGCAT
GTGTCGAGCGCGATCGCGCACGACATGCGCCGGCCGCTGATCGCGCTGCGCAACGCGATCGACGAGGCCAGACGCAGCCC
CGGCGCCGACCCCGCGCTGCGCACCCAGCTCGAAGGGCTGCGCGACCAGACCGAAGAACTGCTGCGGACCTTCTCGGCGC
TGCTCAGCCTTGCGCGCATCGAGGCCGGCGCGCTCGGGCCGCTGCTGCAGGCGGTCAACCTCACCGAGATCGCGCGCGAC
GCGATCGACCTCTACGAACCGCTCGCCGCTTCGCAGGGGCGCGCGCTCAACCCCCGCCTCGCGCCCGCCTGCGTGCGCGG
CGACCGCGACCTGCTGTTCCAGGTGCTGCAGAACCTGATCGAGAACGCGCTCAAGCATGGTGCCGGCGATATCGACATCG
GCATCGACACCCTGCCCGACGGCGGCGCGCGGCTCACCGTGCGCGACCACGGCGAGGCGGTCGCGGCGGCCGACCTGCCG
CATCTGTTCGAGCGCTTCTTCCGCGCCGACAGCAGCCGCAGTGCGGCCGAAGGCGCCGGCATCGGCCTTGCGCTGGTGCG
GGGCATCGCCGAGGCGCACGGCGGCAGCATCCGTGCCACCGACGCGCGCCCCGGCTTGTGCGTCGAAATCCTGCTCGCGC
CGGGAAGGCGCGGGGTCGAGAAGTCCTGA

Upstream 100 bases:

>100_bases
ATCGACGTGCACATCTCCAACCTGCGCGCCAAGATCGACCTGCCCGGGCTGCCGCCGCTGATCCACACCGTGCGTGGTGC
CGGCTACCGGCTGGGCGGCG

Downstream 100 bases:

>100_bases
GCCGCGCCGTTTCAATCCGGTGCGTGCCTTGCGCACCAATGCACAACGGCAGTGCATTAACGCAGCTTCTGGCACGGTAT
TGATGCACTTATTCCGTTGG

Product: putative two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MPRERAGGLRAWFASPFHRFAVALPAAIALIVGALFYPLFIEAEAHIREEVQAAIEFEIAGLEAHFHERGLAGLRDALQR
RIEISPDRAAVYLLADRDGRPLAGNLAQWPAGVSANDEAWFHVAEADGETLEGRVFILFGGERLLVGRRSPLEAFQRNMT
VRLWWSAALIILVAGVIGGRFMQHLHRRLARLAAEAGRIQEGHLAQRLSLSARGDELDALAERFNSAFDEIERLVDAAKH
VSSAIAHDMRRPLIALRNAIDEARRSPGADPALRTQLEGLRDQTEELLRTFSALLSLARIEAGALGPLLQAVNLTEIARD
AIDLYEPLAASQGRALNPRLAPACVRGDRDLLFQVLQNLIENALKHGAGDIDIGIDTLPDGGARLTVRDHGEAVAAADLP
HLFERFFRADSSRSAAEGAGIGLALVRGIAEAHGGSIRATDARPGLCVEILLAPGRRGVEKS

Sequences:

>Translated_462_residues
MPRERAGGLRAWFASPFHRFAVALPAAIALIVGALFYPLFIEAEAHIREEVQAAIEFEIAGLEAHFHERGLAGLRDALQR
RIEISPDRAAVYLLADRDGRPLAGNLAQWPAGVSANDEAWFHVAEADGETLEGRVFILFGGERLLVGRRSPLEAFQRNMT
VRLWWSAALIILVAGVIGGRFMQHLHRRLARLAAEAGRIQEGHLAQRLSLSARGDELDALAERFNSAFDEIERLVDAAKH
VSSAIAHDMRRPLIALRNAIDEARRSPGADPALRTQLEGLRDQTEELLRTFSALLSLARIEAGALGPLLQAVNLTEIARD
AIDLYEPLAASQGRALNPRLAPACVRGDRDLLFQVLQNLIENALKHGAGDIDIGIDTLPDGGARLTVRDHGEAVAAADLP
HLFERFFRADSSRSAAEGAGIGLALVRGIAEAHGGSIRATDARPGLCVEILLAPGRRGVEKS
>Mature_461_residues
PRERAGGLRAWFASPFHRFAVALPAAIALIVGALFYPLFIEAEAHIREEVQAAIEFEIAGLEAHFHERGLAGLRDALQRR
IEISPDRAAVYLLADRDGRPLAGNLAQWPAGVSANDEAWFHVAEADGETLEGRVFILFGGERLLVGRRSPLEAFQRNMTV
RLWWSAALIILVAGVIGGRFMQHLHRRLARLAAEAGRIQEGHLAQRLSLSARGDELDALAERFNSAFDEIERLVDAAKHV
SSAIAHDMRRPLIALRNAIDEARRSPGADPALRTQLEGLRDQTEELLRTFSALLSLARIEAGALGPLLQAVNLTEIARDA
IDLYEPLAASQGRALNPRLAPACVRGDRDLLFQVLQNLIENALKHGAGDIDIGIDTLPDGGARLTVRDHGEAVAAADLPH
LFERFFRADSSRSAAEGAGIGLALVRGIAEAHGGSIRATDARPGLCVEILLAPGRRGVEKS

Specific function: Member of the two-component regulatory system resD/resE involved in the global regulation of aerobic and anaerobic respiration. Probably phosphorylates resD [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=28.3783783783784, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788393, Length=255, Percent_Identity=30.1960784313725, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790346, Length=256, Percent_Identity=26.5625, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787894, Length=267, Percent_Identity=28.4644194756554, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786912, Length=253, Percent_Identity=30.0395256916996, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=207, Percent_Identity=26.5700483091787, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=23.3108108108108, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=32.4074074074074, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790551, Length=255, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1789808, Length=239, Percent_Identity=26.7782426778243, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788549, Length=204, Percent_Identity=27.4509803921569, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790861, Length=289, Percent_Identity=25.2595155709343, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50129; Mature: 49998

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRERAGGLRAWFASPFHRFAVALPAAIALIVGALFYPLFIEAEAHIREEVQAAIEFEIA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
GLEAHFHERGLAGLRDALQRRIEISPDRAAVYLLADRDGRPLAGNLAQWPAGVSANDEAW
CHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCE
FHVAEADGETLEGRVFILFGGERLLVGRRSPLEAFQRNMTVRLWWSAALIILVAGVIGGR
EEEECCCCCEECCEEEEEECCCEEEECCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
FMQHLHRRLARLAAEAGRIQEGHLAQRLSLSARGDELDALAERFNSAFDEIERLVDAAKH
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSSAIAHDMRRPLIALRNAIDEARRSPGADPALRTQLEGLRDQTEELLRTFSALLSLARI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGALGPLLQAVNLTEIARDAIDLYEPLAASQGRALNPRLAPACVRGDRDLLFQVLQNLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHH
ENALKHGAGDIDIGIDTLPDGGARLTVRDHGEAVAAADLPHLFERFFRADSSRSAAEGAG
HHHHHCCCCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
IGLALVRGIAEAHGGSIRATDARPGLCVEILLAPGRRGVEKS
HHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
PRERAGGLRAWFASPFHRFAVALPAAIALIVGALFYPLFIEAEAHIREEVQAAIEFEIA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
GLEAHFHERGLAGLRDALQRRIEISPDRAAVYLLADRDGRPLAGNLAQWPAGVSANDEAW
CHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCE
FHVAEADGETLEGRVFILFGGERLLVGRRSPLEAFQRNMTVRLWWSAALIILVAGVIGGR
EEEECCCCCEECCEEEEEECCCEEEECCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
FMQHLHRRLARLAAEAGRIQEGHLAQRLSLSARGDELDALAERFNSAFDEIERLVDAAKH
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSSAIAHDMRRPLIALRNAIDEARRSPGADPALRTQLEGLRDQTEELLRTFSALLSLARI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGALGPLLQAVNLTEIARDAIDLYEPLAASQGRALNPRLAPACVRGDRDLLFQVLQNLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHH
ENALKHGAGDIDIGIDTLPDGGARLTVRDHGEAVAAADLPHLFERFFRADSSRSAAEGAG
HHHHHCCCCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
IGLALVRGIAEAHGGSIRATDARPGLCVEILLAPGRRGVEKS
HHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 8631715 [H]