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The map label for this gene is ribH

Identifier: 119896609

GI number: 119896609

Start: 343013

End: 343483

Strand: Reverse

Name: ribH

Synonym: azo0318

Alternate gene names: 119896609

Gene position: 343483-343013 (Counterclockwise)

Preceding gene: 119896610

Following gene: 119896608

Centisome position: 7.85

GC content: 64.54

Gene sequence:

>471_bases
ATGGCCCGTTACGACAACATCGCTGAATTCGAATCCGACCTCAACGGCAAAGGCCTGCGCGTCGGCATCGTCATGAGCCG
CTTCAATCAGGACGTCTGCGAAGGGCTGCTGTCCGCCTGTACCGAAGAGCTGCAGAAGCTCGGCGTGTCGCCCGAACTGA
TCCGCATCGCCACCGTGCCCGGCGCGCTGGAAATCCCGCTGGTGCTGCAGAAGATGGGGCAGAGCGGCAAGTTCGACGCG
CTGATCGCGCTCGGCGCGGTGATCCGCGGCGAGACCTACCACTTCGAACTCGTCTCCAACGAAATGGGCGCCGGCATCAC
CCGCATCGGCCTGGACACCGGCATTCCGATCGCCAACGGCGTGCTGACCACCGAAGACGACGACCAGGCGCTGGCCCGCA
TGCAGGAAAAAGGCAGCGACTGCGCGCGCGCCGCGGTGGAGATGGCCAACCTGCTGAAGGTCCTGCAATGA

Upstream 100 bases:

>100_bases
CATGGCCGGCTTCGGCCTCGAAATCACCGGCTTCGTCGAAAAGCCCTGACAACAACGGCGCCCTGCGCGGCGCCTCTCCA
CCCACAGAACACTTCACACC

Downstream 100 bases:

>100_bases
GCAGCCGGATGGCGCGCCGGCGCGCGCGCGAACTCGCGCTGCAGGGCGTTTACCAGTGGCTGCTGTCGGGCAATTCGCCC
CAGGTGGTCGAAGCCCACGT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MARYDNIAEFESDLNGKGLRVGIVMSRFNQDVCEGLLSACTEELQKLGVSPELIRIATVPGALEIPLVLQKMGQSGKFDA
LIALGAVIRGETYHFELVSNEMGAGITRIGLDTGIPIANGVLTTEDDDQALARMQEKGSDCARAAVEMANLLKVLQ

Sequences:

>Translated_156_residues
MARYDNIAEFESDLNGKGLRVGIVMSRFNQDVCEGLLSACTEELQKLGVSPELIRIATVPGALEIPLVLQKMGQSGKFDA
LIALGAVIRGETYHFELVSNEMGAGITRIGLDTGIPIANGVLTTEDDDQALARMQEKGSDCARAAVEMANLLKVLQ
>Mature_155_residues
ARYDNIAEFESDLNGKGLRVGIVMSRFNQDVCEGLLSACTEELQKLGVSPELIRIATVPGALEIPLVLQKMGQSGKFDAL
IALGAVIRGETYHFELVSNEMGAGITRIGLDTGIPIANGVLTTEDDDQALARMQEKGSDCARAAVEMANLLKVLQ

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=152, Percent_Identity=40.1315789473684, Blast_Score=112, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324429, Length=148, Percent_Identity=33.7837837837838, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_AZOSB (A1K280)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931822.1
- ProteinModelPortal:   A1K280
- SMR:   A1K280
- STRING:   A1K280
- GeneID:   4609434
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0318
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   A1K280
- ProtClustDB:   PRK00061
- BioCyc:   ASP62928:AZO0318-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16723; Mature: 16591

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYDNIAEFESDLNGKGLRVGIVMSRFNQDVCEGLLSACTEELQKLGVSPELIRIATVP
CCCCCCHHHHHHCCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GALEIPLVLQKMGQSGKFDALIALGAVIRGETYHFELVSNEMGAGITRIGLDTGIPIANG
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCCCCCC
VLTTEDDDQALARMQEKGSDCARAAVEMANLLKVLQ
EEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ARYDNIAEFESDLNGKGLRVGIVMSRFNQDVCEGLLSACTEELQKLGVSPELIRIATVP
CCCCCHHHHHHCCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GALEIPLVLQKMGQSGKFDALIALGAVIRGETYHFELVSNEMGAGITRIGLDTGIPIANG
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCCCCCC
VLTTEDDDQALARMQEKGSDCARAAVEMANLLKVLQ
EEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA