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The map label for this gene is daaA [H]

Identifier: 119896473

GI number: 119896473

Start: 198136

End: 198993

Strand: Direct

Name: daaA [H]

Synonym: azo0181

Alternate gene names: 119896473

Gene position: 198136-198993 (Clockwise)

Preceding gene: 119896472

Following gene: 119896474

Centisome position: 4.53

GC content: 67.02

Gene sequence:

>858_bases
ATGAATCTGTGCTACCTGGATGGGCGCTACCTGCCGCTCGATGAAGCGCGGGTGTCGCCGATGGATCGCGGTTTCCTGTT
CGGGGACGGCGCCTATGAGGTCGTCCCGGTCTATTCGCGCCGTCCGTTCCGGCTCGCCGAGCATCTTGGCCGCCTTGCCC
GCACGCTGGCGGCAATGCGACTGCCCAACCCGCACTCGGACGAAGAATGGGCGGACCGGGTGACGACCCTGGTGGCGGGC
AACCCGTGGGAGGACCAGTCGATCTACCTGCAGGTCACCCGTGGTGCCGATGCGGTGCGCAACCACGCTTTCCCGAAGGA
CGTCCGGCCCACGGTCTTCATGCTCAGCGAACCCTTGCTGACACCGGCGCCCGAACTGCTCGCCAGCGGGGTCGCGGCGG
TGAGCGCGGCCGACTTCCGCTGGCTGCGGTGCGATCTGAAGAGCGTTTCCCTGCTTGCCAACTGCCTGCTGCGGCAGTAC
GGCTTCGACCAGGGGTGCGCTGAGACGGTGCTCTTTCGCGATGGCTTCCTGACCGAGGGTTCGGCCTCCAACATCTTCGT
CGTGCGGGACGGACGGCTGCTGGCGCCGCCGAAGAGCCACCTGATGCTCGCCGGCATTACCTACGACGTCGTGCTGGAAC
TGGCCGCCGCGCACGGGCTGCCATTGGAGGTGCGCGAAATCCTCGACGCCGAGGTCCGCAGCGCCGACGAACTGTGGATG
ACCTCGTCGACCAAGGAAGTGCTGGCGATCACGCGGCTCGACGGCCGTCCGGTCGGCAGCGGTGCGCCCGGGCCGCTGGG
GCGGCAGATGTACGCCTGGTATCAGGAATTCAAGAACACGGTGATGCGTAGTGGCTGA

Upstream 100 bases:

>100_bases
GGTGGCGGGCTTCTTCGGCCGCCTGTGGGACGCCCTGGTGCTGTGGATCAAGAGCCTGTAAGACGGCGCACCCGCGCCGG
CGGGACTGAAGGACGCACGC

Downstream 100 bases:

>100_bases
TCAGACTCCCCAACCCAGACAAACCCTGCTCGAGTTTCCGTGCGACTTCCCGATCAAGATCATGGGCGCGCGCGTGGACG
GTTTCGCGCAGGCGGTGATC

Product: D-alanine transaminase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNLCYLDGRYLPLDEARVSPMDRGFLFGDGAYEVVPVYSRRPFRLAEHLGRLARTLAAMRLPNPHSDEEWADRVTTLVAG
NPWEDQSIYLQVTRGADAVRNHAFPKDVRPTVFMLSEPLLTPAPELLASGVAAVSAADFRWLRCDLKSVSLLANCLLRQY
GFDQGCAETVLFRDGFLTEGSASNIFVVRDGRLLAPPKSHLMLAGITYDVVLELAAAHGLPLEVREILDAEVRSADELWM
TSSTKEVLAITRLDGRPVGSGAPGPLGRQMYAWYQEFKNTVMRSG

Sequences:

>Translated_285_residues
MNLCYLDGRYLPLDEARVSPMDRGFLFGDGAYEVVPVYSRRPFRLAEHLGRLARTLAAMRLPNPHSDEEWADRVTTLVAG
NPWEDQSIYLQVTRGADAVRNHAFPKDVRPTVFMLSEPLLTPAPELLASGVAAVSAADFRWLRCDLKSVSLLANCLLRQY
GFDQGCAETVLFRDGFLTEGSASNIFVVRDGRLLAPPKSHLMLAGITYDVVLELAAAHGLPLEVREILDAEVRSADELWM
TSSTKEVLAITRLDGRPVGSGAPGPLGRQMYAWYQEFKNTVMRSG
>Mature_285_residues
MNLCYLDGRYLPLDEARVSPMDRGFLFGDGAYEVVPVYSRRPFRLAEHLGRLARTLAAMRLPNPHSDEEWADRVTTLVAG
NPWEDQSIYLQVTRGADAVRNHAFPKDVRPTVFMLSEPLLTPAPELLASGVAAVSAADFRWLRCDLKSVSLLANCLLRQY
GFDQGCAETVLFRDGFLTEGSASNIFVVRDGRLLAPPKSHLMLAGITYDVVLELAAAHGLPLEVREILDAEVRSADELWM
TSSTKEVLAITRLDGRPVGSGAPGPLGRQMYAWYQEFKNTVMRSG

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=290, Percent_Identity=25.8620689655172, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31584; Mature: 31584

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLCYLDGRYLPLDEARVSPMDRGFLFGDGAYEVVPVYSRRPFRLAEHLGRLARTLAAMR
CCEEEECCEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHC
LPNPHSDEEWADRVTTLVAGNPWEDQSIYLQVTRGADAVRNHAFPKDVRPTVFMLSEPLL
CCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHCCCCCCCCCEEEEECCCCC
TPAPELLASGVAAVSAADFRWLRCDLKSVSLLANCLLRQYGFDQGCAETVLFRDGFLTEG
CCCHHHHHHHHHHHHHHCHHEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCC
SASNIFVVRDGRLLAPPKSHLMLAGITYDVVLELAAAHGLPLEVREILDAEVRSADELWM
CCCCEEEEECCEEECCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHC
TSSTKEVLAITRLDGRPVGSGAPGPLGRQMYAWYQEFKNTVMRSG
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLCYLDGRYLPLDEARVSPMDRGFLFGDGAYEVVPVYSRRPFRLAEHLGRLARTLAAMR
CCEEEECCEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHC
LPNPHSDEEWADRVTTLVAGNPWEDQSIYLQVTRGADAVRNHAFPKDVRPTVFMLSEPLL
CCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHCCCCCCCCCEEEEECCCCC
TPAPELLASGVAAVSAADFRWLRCDLKSVSLLANCLLRQYGFDQGCAETVLFRDGFLTEG
CCCHHHHHHHHHHHHHHCHHEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCC
SASNIFVVRDGRLLAPPKSHLMLAGITYDVVLELAAAHGLPLEVREILDAEVRSADELWM
CCCCEEEEECCEEECCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHC
TSSTKEVLAITRLDGRPVGSGAPGPLGRQMYAWYQEFKNTVMRSG
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]