Click here to switch to the map view.

The map label for this gene is kipI [H]

Identifier: 119896337

GI number: 119896337

Start: 50668

End: 51462

Strand: Reverse

Name: kipI [H]

Synonym: azo0045

Alternate gene names: 119896337

Gene position: 51462-50668 (Counterclockwise)

Preceding gene: 119896338

Following gene: 119896336

Centisome position: 1.18

GC content: 73.71

Gene sequence:

>795_bases
ATGAAGCCGCGCGTGCTGGATGCCGGCGATGCGGCGTTCACGGTCGAGTTCGGCGACGCGATCGAGCCGCGGCTGCTCGC
GGCGGTCAACGCGCTGGATGCCGCGATCGCCCGCCTGCAGGCCGATGGCGGCCTGCCCGGCGTGATTGAGACCATGCCCA
CCTTCCGCTCGCTGACGGTGTTCTTCGATCCGCTGGTGACCGGCCGCGCCGCGCTGCTGGCGGCGCTGCAGCCCTTGCTG
GCGGGCGATGCGGACAGGGCGGCGGCGCCGGGCCGGCGCTGGCGGCTGCCGGTGTGCTACGAGGGTGAGGCGGCGCCCGA
CCTTGCCGTCACCGCGCGTGCCACCGGGCTGGACGAGGCCGCGGTGGTCGCGCTGCACAGCGGCACCGAATACCGGGTCT
ACATGCTGGGCTTTCTGCCCGGTTTTCCGTTCATGGGCGACCTGCCGGAACCGCTGCGTCTGCCGCGCCGGGCCGAGCCG
CGGCTGCGCGTGCCGTCCGGCAGCGTCGCCATTGCCACCGGTCTTACCGCGATCTACCCATGGGAAAGCCCCGGCGGCTG
GCATCTGCTCGGGCGCTGCCCGGTGCCGCTGTTCGATGTCCGGCGTGCCGCGCCCGCGCTGCTCGCGGCGGGTGACCGGG
TGGTCTTTGCGCCAGTGTCGGGCGCGGAATATGTCCGCCTGGCTGCCGCGCTGCAGGGCGGCGAAATCGACCCGCAGGAA
TGGCTGTGTTCCGGCGTGGCCGAGGGGGACGGAACCGCGGCCCCTTCGGCCGCGCCGGAGGGTGGGGCGCGATGA

Upstream 100 bases:

>100_bases
GTCCGGCCGATAGGTTCCGGCGGGTGTCCATCCCGTCCGCCGTGCTTCCCGTGAATCCCCGCCCCCCCGCCGGCGGGTGC
CCGCCGCAGGGAGCGGCGCA

Downstream 100 bases:

>100_bases
GCGCGGTGCAGCTCGAAGTGCTCGCGCCCGGCGCCTACGCCTCGCTGCAGGACGGCGGGCGGCGTGGCTATCGGCGTATC
GGCGTGCCCTGGGCGGGTGT

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKPRVLDAGDAAFTVEFGDAIEPRLLAAVNALDAAIARLQADGGLPGVIETMPTFRSLTVFFDPLVTGRAALLAALQPLL
AGDADRAAAPGRRWRLPVCYEGEAAPDLAVTARATGLDEAAVVALHSGTEYRVYMLGFLPGFPFMGDLPEPLRLPRRAEP
RLRVPSGSVAIATGLTAIYPWESPGGWHLLGRCPVPLFDVRRAAPALLAAGDRVVFAPVSGAEYVRLAAALQGGEIDPQE
WLCSGVAEGDGTAAPSAAPEGGAR

Sequences:

>Translated_264_residues
MKPRVLDAGDAAFTVEFGDAIEPRLLAAVNALDAAIARLQADGGLPGVIETMPTFRSLTVFFDPLVTGRAALLAALQPLL
AGDADRAAAPGRRWRLPVCYEGEAAPDLAVTARATGLDEAAVVALHSGTEYRVYMLGFLPGFPFMGDLPEPLRLPRRAEP
RLRVPSGSVAIATGLTAIYPWESPGGWHLLGRCPVPLFDVRRAAPALLAAGDRVVFAPVSGAEYVRLAAALQGGEIDPQE
WLCSGVAEGDGTAAPSAAPEGGAR
>Mature_264_residues
MKPRVLDAGDAAFTVEFGDAIEPRLLAAVNALDAAIARLQADGGLPGVIETMPTFRSLTVFFDPLVTGRAALLAALQPLL
AGDADRAAAPGRRWRLPVCYEGEAAPDLAVTARATGLDEAAVVALHSGTEYRVYMLGFLPGFPFMGDLPEPLRLPRRAEP
RLRVPSGSVAIATGLTAIYPWESPGGWHLLGRCPVPLFDVRRAAPALLAAGDRVVFAPVSGAEYVRLAAALQGGEIDPQE
WLCSGVAEGDGTAAPSAAPEGGAR

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=220, Percent_Identity=40.9090909090909, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 27542; Mature: 27542

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRVLDAGDAAFTVEFGDAIEPRLLAAVNALDAAIARLQADGGLPGVIETMPTFRSLTV
CCCCEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHH
FFDPLVTGRAALLAALQPLLAGDADRAAAPGRRWRLPVCYEGEAAPDLAVTARATGLDEA
HHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEECEEEECCCCCCCEEEEEECCCCCCE
AVVALHSGTEYRVYMLGFLPGFPFMGDLPEPLRLPRRAEPRLRVPSGSVAIATGLTAIYP
EEEEEECCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCCCCEECCCCCEEEEECCEEEEC
WESPGGWHLLGRCPVPLFDVRRAAPALLAAGDRVVFAPVSGAEYVRLAAALQGGEIDPQE
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHH
WLCSGVAEGDGTAAPSAAPEGGAR
HHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPRVLDAGDAAFTVEFGDAIEPRLLAAVNALDAAIARLQADGGLPGVIETMPTFRSLTV
CCCCEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHH
FFDPLVTGRAALLAALQPLLAGDADRAAAPGRRWRLPVCYEGEAAPDLAVTARATGLDEA
HHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEECEEEECCCCCCCEEEEEECCCCCCE
AVVALHSGTEYRVYMLGFLPGFPFMGDLPEPLRLPRRAEPRLRVPSGSVAIATGLTAIYP
EEEEEECCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCCCCEECCCCCEEEEECCEEEEC
WESPGGWHLLGRCPVPLFDVRRAAPALLAAGDRVVFAPVSGAEYVRLAAALQGGEIDPQE
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHH
WLCSGVAEGDGTAAPSAAPEGGAR
HHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]