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The map label for this gene is gcvA [H]

Identifier: 119776737

GI number: 119776737

Start: 4242055

End: 4242993

Strand: Reverse

Name: gcvA [H]

Synonym: Sama_3605

Alternate gene names: 119776737

Gene position: 4242993-4242055 (Counterclockwise)

Preceding gene: 119776739

Following gene: 119776736

Centisome position: 98.53

GC content: 50.27

Gene sequence:

>939_bases
ATGACCCAACGCCCCTATAACTTGCCCCCTTTAAACGCATTGAAATCCTTTGAAGCTTTTAGCCGCCATCTTAATTTGCG
GGATGCAGCGCAAGAGTTATGCGTAACAGAGTCGGCGGTGAGCCGGCAGTTAAAGCAACTGGAAGGGTATTTGGGCGTGA
AATTGTTAGAGCGGGTTGGGCGTAACTCCCAGCTAACGGATGTCGGCCAGCATTATTCCAGGGCGCTCAGCCAGGGGCTG
TATGTTATGAGTGAGGCGACCGAACGACTTTTCCCACCGCTTACGCGTTATGGTTTCCGGCAAGTGTTAAAAATAGGGGT
GGGCCCTGTATTTGCCGAATACTGGTTATCCAAACGCTTGGGAGAATTCAGGGCATTACATCCAAATATCGACTTGGAGT
TGCACGTTAATCACAACTTACTTCTGGAAGCGCCATTGGAGCGCGTTGACCTGGAGATTTATACCGGCAACGCGACTCAC
AATGAGTTTCTCTGTGACCGCTTGTTTCAGATCCAGGATTTTGCCGTCTGCAGCCCGTCGTTACTCAAACGGATAGGTCA
TGTGACCAAGGTAAGCGAATTGCGTAAATTCCCTCTGCTGCATGAGGGCTCCACCCACTGGTGGTCTGAATGGTTTAAAA
GTGCGGGTTGCCAACAACAGCCCGCCAAACACGGCCCCATAGTATACGACCAAATCCAGTGTATTAAGCTGGCGCTGGCA
GGTGAGGGGATTGCCTTGGCTGATCATATTTCGACACTCGATCACATTAAAAATGGTGAGCTGGTTCGGCCCACAACACA
TAAATTGATCACGGCACACTGGATTAGTTTGCTGATTAAACCCGAAAAGAGGCAGGAAGCCCCTGTCGTCGCCTTTAGAG
ATTGGTTGCTCGGCAGCATGCAGGAGTTCAAATCGAGCCTGGGGGATTATCACGAATAA

Upstream 100 bases:

>100_bases
TTTCAATATATGAAGTTGATAACAAGATAAAAATTGATTTGATATCCAAGCTCGTTTGAGTTTTAGTCAAATATAGTCGA
TTCCACCTTAAAACAAATAC

Downstream 100 bases:

>100_bases
CCACAAGTACCAACGAGATAGCCGGCTAAAAAATAGGGGGTAACCGCCTTGGCATTTATGTCATATCCCAGGTGCTCACG
AATTATTGGGCACCGTTGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTQRPYNLPPLNALKSFEAFSRHLNLRDAAQELCVTESAVSRQLKQLEGYLGVKLLERVGRNSQLTDVGQHYSRALSQGL
YVMSEATERLFPPLTRYGFRQVLKIGVGPVFAEYWLSKRLGEFRALHPNIDLELHVNHNLLLEAPLERVDLEIYTGNATH
NEFLCDRLFQIQDFAVCSPSLLKRIGHVTKVSELRKFPLLHEGSTHWWSEWFKSAGCQQQPAKHGPIVYDQIQCIKLALA
GEGIALADHISTLDHIKNGELVRPTTHKLITAHWISLLIKPEKRQEAPVVAFRDWLLGSMQEFKSSLGDYHE

Sequences:

>Translated_312_residues
MTQRPYNLPPLNALKSFEAFSRHLNLRDAAQELCVTESAVSRQLKQLEGYLGVKLLERVGRNSQLTDVGQHYSRALSQGL
YVMSEATERLFPPLTRYGFRQVLKIGVGPVFAEYWLSKRLGEFRALHPNIDLELHVNHNLLLEAPLERVDLEIYTGNATH
NEFLCDRLFQIQDFAVCSPSLLKRIGHVTKVSELRKFPLLHEGSTHWWSEWFKSAGCQQQPAKHGPIVYDQIQCIKLALA
GEGIALADHISTLDHIKNGELVRPTTHKLITAHWISLLIKPEKRQEAPVVAFRDWLLGSMQEFKSSLGDYHE
>Mature_311_residues
TQRPYNLPPLNALKSFEAFSRHLNLRDAAQELCVTESAVSRQLKQLEGYLGVKLLERVGRNSQLTDVGQHYSRALSQGLY
VMSEATERLFPPLTRYGFRQVLKIGVGPVFAEYWLSKRLGEFRALHPNIDLELHVNHNLLLEAPLERVDLEIYTGNATHN
EFLCDRLFQIQDFAVCSPSLLKRIGHVTKVSELRKFPLLHEGSTHWWSEWFKSAGCQQQPAKHGPIVYDQIQCIKLALAG
EGIALADHISTLDHIKNGELVRPTTHKLITAHWISLLIKPEKRQEAPVVAFRDWLLGSMQEFKSSLGDYHE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=31.5436241610738, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786448, Length=304, Percent_Identity=27.9605263157895, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=27.6666666666667, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=28.2392026578073, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786401, Length=280, Percent_Identity=26.7857142857143, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=139, Percent_Identity=32.3741007194245, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35607; Mature: 35476

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRPYNLPPLNALKSFEAFSRHLNLRDAAQELCVTESAVSRQLKQLEGYLGVKLLERVG
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RNSQLTDVGQHYSRALSQGLYVMSEATERLFPPLTRYGFRQVLKIGVGPVFAEYWLSKRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GEFRALHPNIDLELHVNHNLLLEAPLERVDLEIYTGNATHNEFLCDRLFQIQDFAVCSPS
HHHHEECCCCCEEEEECCCEEEECCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHH
LLKRIGHVTKVSELRKFPLLHEGSTHWWSEWFKSAGCQQQPAKHGPIVYDQIQCIKLALA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHC
GEGIALADHISTLDHIKNGELVRPTTHKLITAHWISLLIKPEKRQEAPVVAFRDWLLGSM
CCCCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHH
QEFKSSLGDYHE
HHHHHHHCCCCC
>Mature Secondary Structure 
TQRPYNLPPLNALKSFEAFSRHLNLRDAAQELCVTESAVSRQLKQLEGYLGVKLLERVG
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RNSQLTDVGQHYSRALSQGLYVMSEATERLFPPLTRYGFRQVLKIGVGPVFAEYWLSKRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GEFRALHPNIDLELHVNHNLLLEAPLERVDLEIYTGNATHNEFLCDRLFQIQDFAVCSPS
HHHHEECCCCCEEEEECCCEEEECCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHH
LLKRIGHVTKVSELRKFPLLHEGSTHWWSEWFKSAGCQQQPAKHGPIVYDQIQCIKLALA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHC
GEGIALADHISTLDHIKNGELVRPTTHKLITAHWISLLIKPEKRQEAPVVAFRDWLLGSM
CCCCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHH
QEFKSSLGDYHE
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]