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The map label for this gene is menH [H]

Identifier: 119776593

GI number: 119776593

Start: 4090921

End: 4091754

Strand: Direct

Name: menH [H]

Synonym: Sama_3461

Alternate gene names: 119776593

Gene position: 4090921-4091754 (Clockwise)

Preceding gene: 119776592

Following gene: 119776594

Centisome position: 95.0

GC content: 58.39

Gene sequence:

>834_bases
GTGGATACGCAGCCGCTGAAGCCCTGTCTGCTGTTGTTACATGGCTTTTTGGGCAGCGGCAGCGATTGGGATACATTGGC
TCCCGAACTCGAACCCCATTTTCGGCTGCTGACACCGGACCTACCCGGGCATGGCTCAGCACCGTTTGACGTGCGCTTCG
ACGATAAGGCTGAGAATCAGCCTGACGGCAAGGGCATAAGCCCGTTTGACAAGGTTAGCCTTGCCCTGCTCGACTGGCTC
GCCAAGCAGGACGTGACCGAGTTTCACCTGCTGGGTTATTCCCTCGGTGGCCGCCTGGCCCTGCATCTGGCGAGTCTGGC
CCCAAAAAGGCTGCTGAGCCTTAACCTTGAATCCTGTCATCCGGGCCTTGAGAGTGACAGCGCAAGGGGCGAGCGGCTCG
CCGCCGACAGCCTCTGGGCCAAACGACTGGCCACTGAGCCCCTGCCTGAGTTTCTCGATGCCTGGTATCGTCAAGGCGTA
TTTGCCCATCTCGATGACAACGCCCGCCAGGCTCTGGTAACAAGGCGCTGTGCCCAGCATCAAGACAACAACCGCCAGGC
GCTGTTGCAGATGTATCTGGCCACCTCGCTGGCAAATCAACAGGATCTCAGAGCCGTACCAGCCAACTTGCCCTGCCCGG
TGAATCTTTATGTGGGCGAGGAAGACAACAAATTTACCGCCCTCGCCAACCAGTGGCAGCAGCAAGCGGGTATTCACGTA
TATAGAATTGAAGGCGCCGGCCACAATTGTCATGCCGAGCAGCCGAGCCTGTTTGCACGCAGTCTGCTTCAGGGCATATT
CCATCAATCCAGCGCGGATCAATCGCATGTCTGA

Upstream 100 bases:

>100_bases
TCGATGCCTATCAGGCGGCCCTGGAACATCCGGGCGCCTCTGTGATTGAAGTGACAGTGGCCCAGGATCAGGCCAGTAAT
CAGATAAGGAGAATGGCAGA

Downstream 100 bases:

>100_bases
CTCTTCTTTTGCCCTCTATCCCTATCGCATCGCCCTCAGCCGCCCGCTGCCCGTGGGTAAGCAGCGTATTATGCATCGCA
GCGGCTTGGTGATAGAGGTA

Product: alpha/beta fold family hydrolase

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MDTQPLKPCLLLLHGFLGSGSDWDTLAPELEPHFRLLTPDLPGHGSAPFDVRFDDKAENQPDGKGISPFDKVSLALLDWL
AKQDVTEFHLLGYSLGGRLALHLASLAPKRLLSLNLESCHPGLESDSARGERLAADSLWAKRLATEPLPEFLDAWYRQGV
FAHLDDNARQALVTRRCAQHQDNNRQALLQMYLATSLANQQDLRAVPANLPCPVNLYVGEEDNKFTALANQWQQQAGIHV
YRIEGAGHNCHAEQPSLFARSLLQGIFHQSSADQSHV

Sequences:

>Translated_277_residues
MDTQPLKPCLLLLHGFLGSGSDWDTLAPELEPHFRLLTPDLPGHGSAPFDVRFDDKAENQPDGKGISPFDKVSLALLDWL
AKQDVTEFHLLGYSLGGRLALHLASLAPKRLLSLNLESCHPGLESDSARGERLAADSLWAKRLATEPLPEFLDAWYRQGV
FAHLDDNARQALVTRRCAQHQDNNRQALLQMYLATSLANQQDLRAVPANLPCPVNLYVGEEDNKFTALANQWQQQAGIHV
YRIEGAGHNCHAEQPSLFARSLLQGIFHQSSADQSHV
>Mature_277_residues
MDTQPLKPCLLLLHGFLGSGSDWDTLAPELEPHFRLLTPDLPGHGSAPFDVRFDDKAENQPDGKGISPFDKVSLALLDWL
AKQDVTEFHLLGYSLGGRLALHLASLAPKRLLSLNLESCHPGLESDSARGERLAADSLWAKRLATEPLPEFLDAWYRQGV
FAHLDDNARQALVTRRCAQHQDNNRQALLQMYLATSLANQQDLRAVPANLPCPVNLYVGEEDNKFTALANQWQQQAGIHV
YRIEGAGHNCHAEQPSLFARSLLQGIFHQSSADQSHV

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=261, Percent_Identity=38.3141762452107, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 30706; Mature: 30706

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQPLKPCLLLLHGFLGSGSDWDTLAPELEPHFRLLTPDLPGHGSAPFDVRFDDKAENQ
CCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCC
PDGKGISPFDKVSLALLDWLAKQDVTEFHLLGYSLGGRLALHLASLAPKRLLSLNLESCH
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCHHHHHHCCHHHCC
PGLESDSARGERLAADSLWAKRLATEPLPEFLDAWYRQGVFAHLDDNARQALVTRRCAQH
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHC
QDNNRQALLQMYLATSLANQQDLRAVPANLPCPVNLYVGEEDNKFTALANQWQQQAGIHV
CCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEE
YRIEGAGHNCHAEQPSLFARSLLQGIFHQSSADQSHV
EEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDTQPLKPCLLLLHGFLGSGSDWDTLAPELEPHFRLLTPDLPGHGSAPFDVRFDDKAENQ
CCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCC
PDGKGISPFDKVSLALLDWLAKQDVTEFHLLGYSLGGRLALHLASLAPKRLLSLNLESCH
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCHHHHHHCCHHHCC
PGLESDSARGERLAADSLWAKRLATEPLPEFLDAWYRQGVFAHLDDNARQALVTRRCAQH
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHC
QDNNRQALLQMYLATSLANQQDLRAVPANLPCPVNLYVGEEDNKFTALANQWQQQAGIHV
CCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEE
YRIEGAGHNCHAEQPSLFARSLLQGIFHQSSADQSHV
EEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA