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The map label for this gene is menD

Identifier: 119776592

GI number: 119776592

Start: 4089221

End: 4090939

Strand: Direct

Name: menD

Synonym: Sama_3460

Alternate gene names: 119776592

Gene position: 4089221-4090939 (Clockwise)

Preceding gene: 119776591

Following gene: 119776593

Centisome position: 94.96

GC content: 58.17

Gene sequence:

>1719_bases
ATGGATCACTTGCATTCGAGTACTGCCGAACTCAATTTGTTGTGGGGCTCTTTGATTCTGGAGGAGCTCACCCGCCACGG
TGTAATGCATCTGTGCATGGCACCGGGCTCCCGCTCGACGCCACTGACTCTGGCGGCCGCCGCACAGGATAAGCTGACCC
GGCATCTGCATTTTGATGAGCGGGGGTTGGGGTTCCTTGCCCTCGGGTTGGCGAAAGCCAGCCAGGCCCCCGTCGCCATC
ATCACCACATCGGGTACCGCCGTGGCCAACCTGTATCCGGCCATAGTGGAAGCATCCCTCACCCACGTGCCCCTGATTAT
TCTCTCCGGCGACAGACCCTGGGAACTGATTGGCTGTGGCGCCAATCAGGCCATTGAGCAGCTTGGTATTTTTGGCGGTT
ACGCCCGCCAGCTCAATCTGCCGACTCCCGACCTGCGAATTGGTCCGGAAGTGCTGCTCAGCGCCCTTGATGAACAATTG
GCCAATCTCGACCGGCCGCTGCATATCAATTGCATGTACCCCGAGCCCCTGTATCCGTCAGAACACAGGTTCGATCAGTT
CGACAGTTATTTGAGTCGTCTTGGCCAGTGGCAGGATACTCAGGTGCCTTATCTGTCGGTGGCCAATGCCAGCCTCAGCG
CCTTCCCTCCCCGGGATGCCATGATGCGCTTTGTCCATGGCAAAGGGGTGATAGTGGCGGGCACCCTGACCGAAGCCGAA
ACTCCGACAGAACTCATTACCTTGTCGCAAAAACTGGGTTGGCCACTGCTGACCGATGCTCAAAGCCAGCTGCGCCAGCA
TCCGGGGGCCATAGGGCATATCGATCAGCTGCTGCTCAATCCCAGGGCCCGCGCCCTGTTGGATCAGGCCGAACGGGTAC
TGGTATTTGGCGGCCGACTCCTGTCGAAACGCCTAATCAGTTATCTGGCTGAAAAAGACTGGCACAGTTACTGGCAGGTA
TTACCCCATCAGGAAAGACTCGACCCCAGCCACAGCAACAAGCAACTTTGGCTCGGCCGGGCTGGTGATGTCTGCGCCCT
GGAATGGCCACGCTCATCCGAGGCCAACTGGGCGGCGTCGCTTATCAGCCTGAATCAGTCAGTTGAGCAGGACTTTATCC
ATCATATTGATGGCGGCGAATTCGGTGAGGCTCAGGTTATTCGCGCCATCGCCGCCAGCCATACCGCCGAGCAGCAACTC
TTTATCGGTAACAGCCTGCCGGTGCGCCTCTATGATATGTTTGCGCCCATCGGCTGCTGCGCCGCGTCTACCTATACCAA
CAGAGGTGCTTCCGGCATCGATGGTCTTATTGCCACCGCCTGCGGTGTCGCCCGTCATAGCGGACGGCCCACCACCCTGA
TTCTCGGGGATTTGTCTGCGCTGCACGATTTGAACTCTCTGGCGCTCGCCCGGGATTGCCAGTCACCCCTGGTCATAGTT
GTGCTCAACAATGATGGCGGCAATATTTTTAACTTGTTGCCGGTTCCCACAGAAGAGCTTCGCAGCGACTTCTATCGCCT
CAGCCACGGGTTGGAATTTGGCTACGGCGCCGCCATGTTTGGCCTGCCCTACGACCGTGCCGAAGATATGGAGGCCTTTA
TCGATGCCTATCAGGCGGCCCTGGAACATCCGGGCGCCTCTGTGATTGAAGTGACAGTGGCCCAGGATCAGGCCAGTAAT
CAGATAAGGAGAATGGCAGAGTGGATACGCAGCCGCTGA

Upstream 100 bases:

>100_bases
AACAATTAGCGAGACCTAATGCTAATTCTGACAAAATTAGCCTGTTAACCAGCAGGGTTAGGGGTACACTAAGGTCACTG
TTATCCAATCCCTTTTCACC

Downstream 100 bases:

>100_bases
AGCCCTGTCTGCTGTTGTTACATGGCTTTTTGGGCAGCGGCAGCGATTGGGATACATTGGCTCCCGAACTCGAACCCCAT
TTTCGGCTGCTGACACCGGA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MDHLHSSTAELNLLWGSLILEELTRHGVMHLCMAPGSRSTPLTLAAAAQDKLTRHLHFDERGLGFLALGLAKASQAPVAI
ITTSGTAVANLYPAIVEASLTHVPLIILSGDRPWELIGCGANQAIEQLGIFGGYARQLNLPTPDLRIGPEVLLSALDEQL
ANLDRPLHINCMYPEPLYPSEHRFDQFDSYLSRLGQWQDTQVPYLSVANASLSAFPPRDAMMRFVHGKGVIVAGTLTEAE
TPTELITLSQKLGWPLLTDAQSQLRQHPGAIGHIDQLLLNPRARALLDQAERVLVFGGRLLSKRLISYLAEKDWHSYWQV
LPHQERLDPSHSNKQLWLGRAGDVCALEWPRSSEANWAASLISLNQSVEQDFIHHIDGGEFGEAQVIRAIAASHTAEQQL
FIGNSLPVRLYDMFAPIGCCAASTYTNRGASGIDGLIATACGVARHSGRPTTLILGDLSALHDLNSLALARDCQSPLVIV
VLNNDGGNIFNLLPVPTEELRSDFYRLSHGLEFGYGAAMFGLPYDRAEDMEAFIDAYQAALEHPGASVIEVTVAQDQASN
QIRRMAEWIRSR

Sequences:

>Translated_572_residues
MDHLHSSTAELNLLWGSLILEELTRHGVMHLCMAPGSRSTPLTLAAAAQDKLTRHLHFDERGLGFLALGLAKASQAPVAI
ITTSGTAVANLYPAIVEASLTHVPLIILSGDRPWELIGCGANQAIEQLGIFGGYARQLNLPTPDLRIGPEVLLSALDEQL
ANLDRPLHINCMYPEPLYPSEHRFDQFDSYLSRLGQWQDTQVPYLSVANASLSAFPPRDAMMRFVHGKGVIVAGTLTEAE
TPTELITLSQKLGWPLLTDAQSQLRQHPGAIGHIDQLLLNPRARALLDQAERVLVFGGRLLSKRLISYLAEKDWHSYWQV
LPHQERLDPSHSNKQLWLGRAGDVCALEWPRSSEANWAASLISLNQSVEQDFIHHIDGGEFGEAQVIRAIAASHTAEQQL
FIGNSLPVRLYDMFAPIGCCAASTYTNRGASGIDGLIATACGVARHSGRPTTLILGDLSALHDLNSLALARDCQSPLVIV
VLNNDGGNIFNLLPVPTEELRSDFYRLSHGLEFGYGAAMFGLPYDRAEDMEAFIDAYQAALEHPGASVIEVTVAQDQASN
QIRRMAEWIRSR
>Mature_572_residues
MDHLHSSTAELNLLWGSLILEELTRHGVMHLCMAPGSRSTPLTLAAAAQDKLTRHLHFDERGLGFLALGLAKASQAPVAI
ITTSGTAVANLYPAIVEASLTHVPLIILSGDRPWELIGCGANQAIEQLGIFGGYARQLNLPTPDLRIGPEVLLSALDEQL
ANLDRPLHINCMYPEPLYPSEHRFDQFDSYLSRLGQWQDTQVPYLSVANASLSAFPPRDAMMRFVHGKGVIVAGTLTEAE
TPTELITLSQKLGWPLLTDAQSQLRQHPGAIGHIDQLLLNPRARALLDQAERVLVFGGRLLSKRLISYLAEKDWHSYWQV
LPHQERLDPSHSNKQLWLGRAGDVCALEWPRSSEANWAASLISLNQSVEQDFIHHIDGGEFGEAQVIRAIAASHTAEQQL
FIGNSLPVRLYDMFAPIGCCAASTYTNRGASGIDGLIATACGVARHSGRPTTLILGDLSALHDLNSLALARDCQSPLVIV
VLNNDGGNIFNLLPVPTEELRSDFYRLSHGLEFGYGAAMFGLPYDRAEDMEAFIDAYQAALEHPGASVIEVTVAQDQASN
QIRRMAEWIRSR

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=561, Percent_Identity=43.49376114082, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_SHEAM (A1SBA6)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_929332.1
- ProteinModelPortal:   A1SBA6
- STRING:   A1SBA6
- GeneID:   4605707
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_3460
- NMPDR:   fig|326297.7.peg.3259
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- PhylomeDB:   A1SBA6
- ProtClustDB:   PRK07449
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62659; Mature: 62659

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLHSSTAELNLLWGSLILEELTRHGVMHLCMAPGSRSTPLTLAAAAQDKLTRHLHFDE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCC
RGLGFLALGLAKASQAPVAIITTSGTAVANLYPAIVEASLTHVPLIILSGDRPWELIGCG
CCCCEEEEEHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECC
ANQAIEQLGIFGGYARQLNLPTPDLRIGPEVLLSALDEQLANLDRPLHINCMYPEPLYPS
HHHHHHHHHHCCCHHEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
EHRFDQFDSYLSRLGQWQDTQVPYLSVANASLSAFPPRDAMMRFVHGKGVIVAGTLTEAE
CCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCC
TPTELITLSQKLGWPLLTDAQSQLRQHPGAIGHIDQLLLNPRARALLDQAERVLVFGGRL
CCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHEEEEHHHHH
LSKRLISYLAEKDWHSYWQVLPHQERLDPSHSNKQLWLGRAGDVCALEWPRSSEANWAAS
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECCCCEEEEECCCCCCCHHHHH
LISLNQSVEQDFIHHIDGGEFGEAQVIRAIAASHTAEQQLFIGNSLPVRLYDMFAPIGCC
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCHHHH
AASTYTNRGASGIDGLIATACGVARHSGRPTTLILGDLSALHDLNSLALARDCQSPLVIV
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEE
VLNNDGGNIFNLLPVPTEELRSDFYRLSHGLEFGYGAAMFGLPYDRAEDMEAFIDAYQAA
EEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHEECCCCCCHHHHHHHHHHHHHH
LEHPGASVIEVTVAQDQASNQIRRMAEWIRSR
HHCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDHLHSSTAELNLLWGSLILEELTRHGVMHLCMAPGSRSTPLTLAAAAQDKLTRHLHFDE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCC
RGLGFLALGLAKASQAPVAIITTSGTAVANLYPAIVEASLTHVPLIILSGDRPWELIGCG
CCCCEEEEEHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECC
ANQAIEQLGIFGGYARQLNLPTPDLRIGPEVLLSALDEQLANLDRPLHINCMYPEPLYPS
HHHHHHHHHHCCCHHEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
EHRFDQFDSYLSRLGQWQDTQVPYLSVANASLSAFPPRDAMMRFVHGKGVIVAGTLTEAE
CCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCC
TPTELITLSQKLGWPLLTDAQSQLRQHPGAIGHIDQLLLNPRARALLDQAERVLVFGGRL
CCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHEEEEHHHHH
LSKRLISYLAEKDWHSYWQVLPHQERLDPSHSNKQLWLGRAGDVCALEWPRSSEANWAAS
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECCCCEEEEECCCCCCCHHHHH
LISLNQSVEQDFIHHIDGGEFGEAQVIRAIAASHTAEQQLFIGNSLPVRLYDMFAPIGCC
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCHHHH
AASTYTNRGASGIDGLIATACGVARHSGRPTTLILGDLSALHDLNSLALARDCQSPLVIV
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEE
VLNNDGGNIFNLLPVPTEELRSDFYRLSHGLEFGYGAAMFGLPYDRAEDMEAFIDAYQAA
EEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHEECCCCCCHHHHHHHHHHHHHH
LEHPGASVIEVTVAQDQASNQIRRMAEWIRSR
HHCCCCCEEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA