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The map label for this gene is ilvK [H]

Identifier: 119776431

GI number: 119776431

Start: 3925499

End: 3926584

Strand: Reverse

Name: ilvK [H]

Synonym: Sama_3299

Alternate gene names: 119776431

Gene position: 3926584-3925499 (Counterclockwise)

Preceding gene: 119776433

Following gene: 119776424

Centisome position: 91.19

GC content: 54.79

Gene sequence:

>1086_bases
ATGGATATAAAGTACAATTTAAAACCCGCATCGGAGCGCCGCACTGAGCCCTTTGAACCTCAGGGAGACGTGGGATTCGG
CAAGCTTAGAACCGACCATATGTTTATGATGGACTACAAGGATGGCCAGTGGCAGAACGCCCGTATTTTGCCCTACGGCC
CCTTCGAAATGATGCCCGGCGCCATGGCGCTGCATTATGGCCAGTCTATCTTTGAAGGCGCCAAAGCCTTTATGCATGAC
GACGGTGAAATTTACACCTTCCGTCTGGACAAAAACGCCCAGCGTCTCAATCGCTCGGCCGACATTGTCTGTATCCCGAA
TATTCCGGAAGCCGTGCAACTTGAGGCCATCAACGCCCTGATTGATGTGGACCGTCTGTGGTTTCCACGTCAGGAAGGCG
CCTGTCTTTATATCCGCCCCTTTGTATTTGCCACTGAAGACCGCCTGTCGGTGAGCCCGGCGAACCAGTACACCTTCTGT
GTGGTATTGAGCCCAAGCGGTGCCTACTATGGCAAAAACCTCAATGCCATTCGCCTGTTGATCACCACTCAGTTCCACCG
GGCTGTGTCCGGTGGCACAGGGGCTTCCAAGGCCGCCGGTAACTATGCCGCATCCTTGCGTGCCGGTAAGGCTGCCGCCG
AGCATGGCGCAGCCCAGGTGCTGTATCTGGACTCAACCAACCAGTACATTGAAGAAGTGGGCGCCATGAACCACTTCCAT
ATCCGTAAAGACGGCACCCTGATTATTCCTGAGTTTACCGATACCATTTTGCGCTCCATTACCTCTGAGTCGATTCTGGC
GCTGGGTGAAGAGCTCGGCTGCGAAGTGCGTCAGGAGACAGTAAAACTCAGCGATTTTATTGCCGATATCGAAGCCGGTG
AAATCGTAGAGGCCGGTGGTTTTGGTACTGCCGCTGTGGTATCGCCGGTTGGCTCTTACATCTTTGAAGATGGCCGTATC
GTCACCGTGGGTGATGGCAAGGTGGGTGAGCGCATCAGCCACATCTACAAGGTGTATACCGAGATGCAAAAGGGCCTGCG
CCCTGCGCCTGAAGGCTGGGTACGCAAGGTCGAGCGCCGCAGTTAA

Upstream 100 bases:

>100_bases
CTTAACGGTTTACAGCATCTTTTTGTTGGAATTTGCGCCGCTATTGACTTTATCCCTGCTGCTCCCTAGTCTGCAAGGTC
AGTCATCAAGGTGGTATGCA

Downstream 100 bases:

>100_bases
TAATAAGCGCCACCGTTGCTGGCTGGGCACTTCGCCCGCAAGCGAAGCCCACAAAGTAAAGCCAGCAAGGCGAAGCCCAC
ACAGCAAAGCCAGAACAGCA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MDIKYNLKPASERRTEPFEPQGDVGFGKLRTDHMFMMDYKDGQWQNARILPYGPFEMMPGAMALHYGQSIFEGAKAFMHD
DGEIYTFRLDKNAQRLNRSADIVCIPNIPEAVQLEAINALIDVDRLWFPRQEGACLYIRPFVFATEDRLSVSPANQYTFC
VVLSPSGAYYGKNLNAIRLLITTQFHRAVSGGTGASKAAGNYAASLRAGKAAAEHGAAQVLYLDSTNQYIEEVGAMNHFH
IRKDGTLIIPEFTDTILRSITSESILALGEELGCEVRQETVKLSDFIADIEAGEIVEAGGFGTAAVVSPVGSYIFEDGRI
VTVGDGKVGERISHIYKVYTEMQKGLRPAPEGWVRKVERRS

Sequences:

>Translated_361_residues
MDIKYNLKPASERRTEPFEPQGDVGFGKLRTDHMFMMDYKDGQWQNARILPYGPFEMMPGAMALHYGQSIFEGAKAFMHD
DGEIYTFRLDKNAQRLNRSADIVCIPNIPEAVQLEAINALIDVDRLWFPRQEGACLYIRPFVFATEDRLSVSPANQYTFC
VVLSPSGAYYGKNLNAIRLLITTQFHRAVSGGTGASKAAGNYAASLRAGKAAAEHGAAQVLYLDSTNQYIEEVGAMNHFH
IRKDGTLIIPEFTDTILRSITSESILALGEELGCEVRQETVKLSDFIADIEAGEIVEAGGFGTAAVVSPVGSYIFEDGRI
VTVGDGKVGERISHIYKVYTEMQKGLRPAPEGWVRKVERRS
>Mature_361_residues
MDIKYNLKPASERRTEPFEPQGDVGFGKLRTDHMFMMDYKDGQWQNARILPYGPFEMMPGAMALHYGQSIFEGAKAFMHD
DGEIYTFRLDKNAQRLNRSADIVCIPNIPEAVQLEAINALIDVDRLWFPRQEGACLYIRPFVFATEDRLSVSPANQYTFC
VVLSPSGAYYGKNLNAIRLLITTQFHRAVSGGTGASKAAGNYAASLRAGKAAAEHGAAQVLYLDSTNQYIEEVGAMNHFH
IRKDGTLIIPEFTDTILRSITSESILALGEELGCEVRQETVKLSDFIADIEAGEIVEAGGFGTAAVVSPVGSYIFEDGRI
VTVGDGKVGERISHIYKVYTEMQKGLRPAPEGWVRKVERRS

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=354, Percent_Identity=33.3333333333333, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI38176287, Length=347, Percent_Identity=33.7175792507205, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI296010904, Length=347, Percent_Identity=33.7175792507205, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI296010906, Length=347, Percent_Identity=33.7175792507205, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI258614015, Length=287, Percent_Identity=33.7979094076655, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI296010902, Length=347, Percent_Identity=30.2593659942363, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI296010900, Length=347, Percent_Identity=30.2593659942363, Blast_Score=138, Evalue=7e-33,
Organism=Escherichia coli, GI48994963, Length=321, Percent_Identity=31.1526479750779, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=371, Percent_Identity=32.0754716981132, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17565728, Length=360, Percent_Identity=31.9444444444444, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322608, Length=303, Percent_Identity=35.6435643564356, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322002, Length=321, Percent_Identity=32.7102803738318, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24641779, Length=344, Percent_Identity=33.1395348837209, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39991; Mature: 39991

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKYNLKPASERRTEPFEPQGDVGFGKLRTDHMFMMDYKDGQWQNARILPYGPFEMMPG
CCCEECCCCCHHCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCEEECCCCHHHCCC
AMALHYGQSIFEGAKAFMHDDGEIYTFRLDKNAQRLNRSADIVCIPNIPEAVQLEAINAL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHCCCCCEEEECCCCCHHHHHHHHHH
IDVDRLWFPRQEGACLYIRPFVFATEDRLSVSPANQYTFCVVLSPSGAYYGKNLNAIRLL
HHHHHHCCCCCCCCEEEEEEEEEEECCCCEECCCCCEEEEEEECCCCCCCCCCCCEEEEE
ITTQFHRAVSGGTGASKAAGNYAASLRAGKAAAEHGAAQVLYLDSTNQYIEEVGAMNHFH
EEHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHCCCEEEEEECCCHHHHHHHCCCCEEE
IRKDGTLIIPEFTDTILRSITSESILALGEELGCEVRQETVKLSDFIADIEAGEIVEAGG
EECCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCC
FGTAAVVSPVGSYIFEDGRIVTVGDGKVGERISHIYKVYTEMQKGLRPAPEGWVRKVERR
CCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
S
C
>Mature Secondary Structure
MDIKYNLKPASERRTEPFEPQGDVGFGKLRTDHMFMMDYKDGQWQNARILPYGPFEMMPG
CCCEECCCCCHHCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCEEECCCCHHHCCC
AMALHYGQSIFEGAKAFMHDDGEIYTFRLDKNAQRLNRSADIVCIPNIPEAVQLEAINAL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHCCCCCEEEECCCCCHHHHHHHHHH
IDVDRLWFPRQEGACLYIRPFVFATEDRLSVSPANQYTFCVVLSPSGAYYGKNLNAIRLL
HHHHHHCCCCCCCCEEEEEEEEEEECCCCEECCCCCEEEEEEECCCCCCCCCCCCEEEEE
ITTQFHRAVSGGTGASKAAGNYAASLRAGKAAAEHGAAQVLYLDSTNQYIEEVGAMNHFH
EEHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHCCCEEEEEECCCHHHHHHHCCCCEEE
IRKDGTLIIPEFTDTILRSITSESILALGEELGCEVRQETVKLSDFIADIEAGEIVEAGG
EECCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCC
FGTAAVVSPVGSYIFEDGRIVTVGDGKVGERISHIYKVYTEMQKGLRPAPEGWVRKVERR
CCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]