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The map label for this gene is metF [H]

Identifier: 119776301

GI number: 119776301

Start: 3756219

End: 3757109

Strand: Reverse

Name: metF [H]

Synonym: Sama_3169

Alternate gene names: 119776301

Gene position: 3757109-3756219 (Counterclockwise)

Preceding gene: 119776302

Following gene: 119776300

Centisome position: 87.25

GC content: 54.43

Gene sequence:

>891_bases
ATGGCATTTCATCACGCACAACACGCAAACTCACTGAACCAGAATCTGGCTGAGGTTGGCGATATCAATGTGTCATTTGA
ATTTTTTCCGCCCTCAACTCCTGAGATGGAAAAGATCCTGTGGAACTCCATCCGCCGCCTCGAGCCGCTGAATCCCAAGT
TTGTGTCTGTAACCTACGGTGCCAACTCGGGTGTCCGTGACCGCACCCACGGTGTGATTGAGCGTATCCAGCAAGAAACC
CAACTGGTTGCCGCCCCGCACCTTACGCTGGTGGATGCCAGCGATGAAGAGTTGTTGGCCCTGGCCAAAAATTACTGGCA
TCAGGGCATTCGCAATATTGTTGCATTGCGTGGTGACTTGCCTGCCGGCAGCCCCAAGCCAACCCGCTTTGCCGCCGATC
TGGTGCGTTTGCTGAAGAGTGTGGCCGACTTTGATATATCGGTGGCAGCCTATCCGGAGGTGCATCCGGAAGCTGCCAAT
GCCCAGGCGGATTTGGTTAATCTCAAGCGCAAAATCGATGCCGGTGCCAACCGTGCCATCACCCAGTTTTTCTTCGACGT
AGAATCCTATTTGCGCTTCCGTGACCGTTGTGTGGCCGCAGGTATTGATGTGGAAATCGTACCGGGCATTCTGCCTGTGA
CCAACTTTACCCAGCTCAAGCGTTTTGCAGCTATGACCAACGTGAGTATTCCCGGTTGGTTGCATCGCCAGTTTGAGGGC
CTGGAAAATGACGCAGCTACCCGTCAGTTGGTGGGGGCCAACGTGGCCATCGATATGGTGAAGGTATTGGCCCGTGAAGG
CGTGAAAGACTTCCACTTTTACACCCTGAACCGTGCCGAGCTGAGCTACGCCATTTGCCATACCTTGGGGGTACGTCCGA
AGCAGGACTGA

Upstream 100 bases:

>100_bases
AAGATCGGGCCATTTTTTAACTTGACTTAGTGACGGGAATCTCTAGTATATGGCCATATAGACGTCCAAACGTCCATTTG
AAAAATTGAGGGAGTTATCA

Downstream 100 bases:

>100_bases
TTTTTATCCGAATAAGGTCTGGCAGGGCTCCTTTTTGAAGGAGCCTTTTTGGTTTTTGGCGGTTCTTACGCTATGGTTTT
AGTCACTAAGGTAAGGGGGT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MAFHHAQHANSLNQNLAEVGDINVSFEFFPPSTPEMEKILWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQQET
QLVAAPHLTLVDASDEELLALAKNYWHQGIRNIVALRGDLPAGSPKPTRFAADLVRLLKSVADFDISVAAYPEVHPEAAN
AQADLVNLKRKIDAGANRAITQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAAMTNVSIPGWLHRQFEG
LENDAATRQLVGANVAIDMVKVLAREGVKDFHFYTLNRAELSYAICHTLGVRPKQD

Sequences:

>Translated_296_residues
MAFHHAQHANSLNQNLAEVGDINVSFEFFPPSTPEMEKILWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQQET
QLVAAPHLTLVDASDEELLALAKNYWHQGIRNIVALRGDLPAGSPKPTRFAADLVRLLKSVADFDISVAAYPEVHPEAAN
AQADLVNLKRKIDAGANRAITQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAAMTNVSIPGWLHRQFEG
LENDAATRQLVGANVAIDMVKVLAREGVKDFHFYTLNRAELSYAICHTLGVRPKQD
>Mature_295_residues
AFHHAQHANSLNQNLAEVGDINVSFEFFPPSTPEMEKILWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQQETQ
LVAAPHLTLVDASDEELLALAKNYWHQGIRNIVALRGDLPAGSPKPTRFAADLVRLLKSVADFDISVAAYPEVHPEAANA
QADLVNLKRKIDAGANRAITQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAAMTNVSIPGWLHRQFEGL
ENDAATRQLVGANVAIDMVKVLAREGVKDFHFYTLNRAELSYAICHTLGVRPKQD

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=287, Percent_Identity=33.7979094076655, Blast_Score=141, Evalue=6e-34,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=74.1496598639456, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI25147436, Length=283, Percent_Identity=30.7420494699647, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI25147441, Length=283, Percent_Identity=30.7420494699647, Blast_Score=130, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=32.9931972789116, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321313, Length=297, Percent_Identity=30.6397306397306, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24662658, Length=264, Percent_Identity=27.6515151515151, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32979; Mature: 32848

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFHHAQHANSLNQNLAEVGDINVSFEFFPPSTPEMEKILWNSIRRLEPLNPKFVSVTYG
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEC
ANSGVRDRTHGVIERIQQETQLVAAPHLTLVDASDEELLALAKNYWHQGIRNIVALRGDL
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
PAGSPKPTRFAADLVRLLKSVADFDISVAAYPEVHPEAANAQADLVNLKRKIDAGANRAI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
TQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAAMTNVSIPGWLHRQFEG
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHC
LENDAATRQLVGANVAIDMVKVLAREGVKDFHFYTLNRAELSYAICHTLGVRPKQD
CCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEECHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AFHHAQHANSLNQNLAEVGDINVSFEFFPPSTPEMEKILWNSIRRLEPLNPKFVSVTYG
CCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEC
ANSGVRDRTHGVIERIQQETQLVAAPHLTLVDASDEELLALAKNYWHQGIRNIVALRGDL
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
PAGSPKPTRFAADLVRLLKSVADFDISVAAYPEVHPEAANAQADLVNLKRKIDAGANRAI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
TQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAAMTNVSIPGWLHRQFEG
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHC
LENDAATRQLVGANVAIDMVKVLAREGVKDFHFYTLNRAELSYAICHTLGVRPKQD
CCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEECHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]