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The map label for this gene is metL [H]

Identifier: 119776302

GI number: 119776302

Start: 3757284

End: 3759677

Strand: Reverse

Name: metL [H]

Synonym: Sama_3170

Alternate gene names: 119776302

Gene position: 3759677-3757284 (Counterclockwise)

Preceding gene: 119776303

Following gene: 119776301

Centisome position: 87.31

GC content: 57.1

Gene sequence:

>2394_bases
ATGGCGCGTTGTCATTTGCATAAGTTTGGTGGCTCCAGTTTGGCGGATGCCGATTGCTACCGCCGCGTGGCCCATATCCT
GTTGACCCAGGGCCACAGCGACGATCTGGTGGTGGTATCAGCCGCCGGTAAAACCACCAACTTTTTGTATAAGCTGCTTT
CGCTGCGTGATGCCGGTGAGTTGTGGCAGGAAGAGTTACAGGTACTGATTAGCTATCAGCAAAACCTGATTGAACAGTTA
CTCAGTGCCGAGCAGGCTCGCTATCTGCGTGAGCGTCTGTCGACCGACAAGGCTCAGCTGGTCAGTCTCCTGTCATTGGC
GACTCTGAACGATTATCAGACCAGCCATGTGGTGAGCTTTGGTGAGCGTTGGTCCGCCAGGTTGATGGCGGCACTCTTGC
GTGAATCCGGCGTGGCGGCAAGCCACGTGGATGCCTGCAGCATACTGGTGGCAGATGAAGGCGCCGTGCCCAATATTCGC
GAGCAGGAGTCCCGCGAAAAAGTAGCTGCCATGCTGGCCGAACATGAAAACGAACGCATTGTTATCACGGGCTTTATCTG
CGCCAATCCGGCCGGTGAAACCCTGTTGCTTGGGCGTAACGGCTCTGACTTTTCCGCGACCCTGATAGCCAGTCTGGCAG
ATATCGAACGGGTCACCATCTGGACTGATGTGGAAGGGGTGTTCAACGCCGACCCGAATAAGATAAACGATGCCAAGCTG
CTCAAGAGCATGTCACTGGCGGAGGCCGACCGCCTGGCGCGTTTGGGTTCTCCTGTGTTACACAGCCGCACTTTGCAGCC
CCTGTTCAACACCCAGGTGAGCATGGCAGTGCGCTCAAGCTACGCGCCTCATACCGATTTTACTCTGGTTGCTCCAACCA
GCTCTCAGGCCAGTGCCCCGGTTGTCACCAATCTGAGCGAAGTGGCGCTCTTTAGCCTCACCACCCAGGCAGACACCGAT
GCCCTGTTGGCAACATTGTCTGCCTCTGGCTTAACGCCGTTGGCAAGCTGGCAGGGTTCCAGGGGTGGGCTGGAACTGGC
CTATACTCCGGAAAATCTTAAGCAGGTCAGCGCCTTTTTAAAGGCGAACGCCGAGGCATATGGTCTGGGCGATGTTTCCA
TCAGCAACGATTTTGGTTTGGTGGCACTGGTGAGCGCCGATGCGGTACTGTACCGCAAGAGTTTTGCCCGCCTGCTCAGT
CGCGAGGCCAAGCCTTTGTATCATGATACCCTGAGCCTGGTGACTCTGGTGCCAAAGGCGCAGGTGAACCTGCTTACGCA
AAAGGTGCACCGCCGCTGCGCCGGGCCACGCAAGCGTATTGGCGTCATTCTCCTTGGTGTTGGCAACATCGGCGAAGCCT
GGGTAAGCCTGTTTCGCCGGGCCCAAAGCAGTCTGTGCCGTGAGCTGGAGGCGCGGATTGAACTCGTGGGGCTGGTCAGC
TCTTCCCGCGCCTACATCAACAATGCCGGTGTGCACCTGGAGAACTGGAAAACCGAATTTGAGGAGTACGCCACCGACTG
GCAGTACGGGCAGTTGTTCGAAGCCTTATCGACACTGAATTGCGATGAGCTGGTTGCGCTGGACATCAGTGCCAGTGCCA
GCCTGACCTTGCAGTACCCGGAGTTTTTTGCCCGTGGTATTCATGTGGTGAGCGCAAATAAGCTGGCCGGTTCTGGTCCC
TTGCCCTTTTATCGCGATCTCAAGCAGCAGTTGGGTAACCGTCGTCTCTATTGGCGCTACAACGCCAGCTGCGGTGCCGG
TTTGCCGGTGCAGCATGCGCTTAACGATTTGCACAACAGTGGCGACAGCGTAGAAGCCGTGGGCGGGATATTTTCCGGTA
CTCTCTGTTGGCTTTTTGAACACTATGACGGTCAGCGTCCTTTCTCTGATTTGGTCATTGAGGCCCGCGGCCTGGGTATC
ACTGAGCCGGACCCGAGGGACGATCTTTCCGGTCGTGATATGCAGCGCAAGCTGCTCATCCTTGCGCGGGAAATTGGCCT
GGATCTGGAGCTGGATGATATTCAGGTCGAGTCTCCTGTACCGCCGCATCTGGCGGAGCTGACGCTGGATGAGTTTCTTG
CCCGCATTGATGAACTCGATGCCCCCATGGCGGATGCCTTGGCGGCTGCCGCAGCCGAGGGCAAGGTGCTGCGTTATATC
GCTGCGCTGGACAGAGCCGGTGGTCAGTTAATGGCACGGGTAGGGTTGCAGTGGGTCGACAAAGCCCACCCTTATGCCAA
TCTCACCCCGGGCGACAATGTGTTTGTCATTCGCAGTACCTTTTACCAGGGTAATCCTTTGATTATCAGGGGGCCCGGTG
CCGGTCGTGAGGTAACAGCCGCGGCTGTTCAGTCAGATCTGGTGCAGATCTGTCGGGATCTCTTGCAGGATTGA

Upstream 100 bases:

>100_bases
CTGCTGCGACTGTCGGTGGGGATTGAAGATGCAGAAGATTTAGTGGCCGATATCCAAGCCGGCCTGGCTGCCGTGGCAGC
CTGTTGAATAGGAGTCAGTG

Downstream 100 bases:

>100_bases
TTTTATAAGATATTGAATAACAGGGACTCTTTTGAGTCCCTGTGTTTTTTCCGCCTTCTGCTCGCAAAAAGGGCAAGATC
GGGCCATTTTTTAACTTGAC

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 797; Mature: 796

Protein sequence:

>797_residues
MARCHLHKFGGSSLADADCYRRVAHILLTQGHSDDLVVVSAAGKTTNFLYKLLSLRDAGELWQEELQVLISYQQNLIEQL
LSAEQARYLRERLSTDKAQLVSLLSLATLNDYQTSHVVSFGERWSARLMAALLRESGVAASHVDACSILVADEGAVPNIR
EQESREKVAAMLAEHENERIVITGFICANPAGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
LKSMSLAEADRLARLGSPVLHSRTLQPLFNTQVSMAVRSSYAPHTDFTLVAPTSSQASAPVVTNLSEVALFSLTTQADTD
ALLATLSASGLTPLASWQGSRGGLELAYTPENLKQVSAFLKANAEAYGLGDVSISNDFGLVALVSADAVLYRKSFARLLS
REAKPLYHDTLSLVTLVPKAQVNLLTQKVHRRCAGPRKRIGVILLGVGNIGEAWVSLFRRAQSSLCRELEARIELVGLVS
SSRAYINNAGVHLENWKTEFEEYATDWQYGQLFEALSTLNCDELVALDISASASLTLQYPEFFARGIHVVSANKLAGSGP
LPFYRDLKQQLGNRRLYWRYNASCGAGLPVQHALNDLHNSGDSVEAVGGIFSGTLCWLFEHYDGQRPFSDLVIEARGLGI
TEPDPRDDLSGRDMQRKLLILAREIGLDLELDDIQVESPVPPHLAELTLDEFLARIDELDAPMADALAAAAAEGKVLRYI
AALDRAGGQLMARVGLQWVDKAHPYANLTPGDNVFVIRSTFYQGNPLIIRGPGAGREVTAAAVQSDLVQICRDLLQD

Sequences:

>Translated_797_residues
MARCHLHKFGGSSLADADCYRRVAHILLTQGHSDDLVVVSAAGKTTNFLYKLLSLRDAGELWQEELQVLISYQQNLIEQL
LSAEQARYLRERLSTDKAQLVSLLSLATLNDYQTSHVVSFGERWSARLMAALLRESGVAASHVDACSILVADEGAVPNIR
EQESREKVAAMLAEHENERIVITGFICANPAGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
LKSMSLAEADRLARLGSPVLHSRTLQPLFNTQVSMAVRSSYAPHTDFTLVAPTSSQASAPVVTNLSEVALFSLTTQADTD
ALLATLSASGLTPLASWQGSRGGLELAYTPENLKQVSAFLKANAEAYGLGDVSISNDFGLVALVSADAVLYRKSFARLLS
REAKPLYHDTLSLVTLVPKAQVNLLTQKVHRRCAGPRKRIGVILLGVGNIGEAWVSLFRRAQSSLCRELEARIELVGLVS
SSRAYINNAGVHLENWKTEFEEYATDWQYGQLFEALSTLNCDELVALDISASASLTLQYPEFFARGIHVVSANKLAGSGP
LPFYRDLKQQLGNRRLYWRYNASCGAGLPVQHALNDLHNSGDSVEAVGGIFSGTLCWLFEHYDGQRPFSDLVIEARGLGI
TEPDPRDDLSGRDMQRKLLILAREIGLDLELDDIQVESPVPPHLAELTLDEFLARIDELDAPMADALAAAAAEGKVLRYI
AALDRAGGQLMARVGLQWVDKAHPYANLTPGDNVFVIRSTFYQGNPLIIRGPGAGREVTAAAVQSDLVQICRDLLQD
>Mature_796_residues
ARCHLHKFGGSSLADADCYRRVAHILLTQGHSDDLVVVSAAGKTTNFLYKLLSLRDAGELWQEELQVLISYQQNLIEQLL
SAEQARYLRERLSTDKAQLVSLLSLATLNDYQTSHVVSFGERWSARLMAALLRESGVAASHVDACSILVADEGAVPNIRE
QESREKVAAMLAEHENERIVITGFICANPAGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKLL
KSMSLAEADRLARLGSPVLHSRTLQPLFNTQVSMAVRSSYAPHTDFTLVAPTSSQASAPVVTNLSEVALFSLTTQADTDA
LLATLSASGLTPLASWQGSRGGLELAYTPENLKQVSAFLKANAEAYGLGDVSISNDFGLVALVSADAVLYRKSFARLLSR
EAKPLYHDTLSLVTLVPKAQVNLLTQKVHRRCAGPRKRIGVILLGVGNIGEAWVSLFRRAQSSLCRELEARIELVGLVSS
SRAYINNAGVHLENWKTEFEEYATDWQYGQLFEALSTLNCDELVALDISASASLTLQYPEFFARGIHVVSANKLAGSGPL
PFYRDLKQQLGNRRLYWRYNASCGAGLPVQHALNDLHNSGDSVEAVGGIFSGTLCWLFEHYDGQRPFSDLVIEARGLGIT
EPDPRDDLSGRDMQRKLLILAREIGLDLELDDIQVESPVPPHLAELTLDEFLARIDELDAPMADALAAAAAEGKVLRYIA
ALDRAGGQLMARVGLQWVDKAHPYANLTPGDNVFVIRSTFYQGNPLIIRGPGAGREVTAAAVQSDLVQICRDLLQD

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=805, Percent_Identity=42.360248447205, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1786183, Length=338, Percent_Identity=37.5739644970414, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1790455, Length=294, Percent_Identity=35.3741496598639, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322599, Length=375, Percent_Identity=33.0666666666667, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6320893, Length=311, Percent_Identity=26.6881028938907, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 86900; Mature: 86768

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCHLHKFGGSSLADADCYRRVAHILLTQGHSDDLVVVSAAGKTTNFLYKLLSLRDAGE
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCHHH
LWQEELQVLISYQQNLIEQLLSAEQARYLRERLSTDKAQLVSLLSLATLNDYQTSHVVSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHH
GERWSARLMAALLRESGVAASHVDACSILVADEGAVPNIREQESREKVAAMLAEHENERI
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEE
VITGFICANPAGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
EEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHH
LKSMSLAEADRLARLGSPVLHSRTLQPLFNTQVSMAVRSSYAPHTDFTLVAPTSSQASAP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
VVTNLSEVALFSLTTQADTDALLATLSASGLTPLASWQGSRGGLELAYTPENLKQVSAFL
HHHCHHHHHEEEEECCCCHHHHHHHHHCCCCCCHHHCCCCCCCEEEEECHHHHHHHHHHH
KANAEAYGLGDVSISNDFGLVALVSADAVLYRKSFARLLSREAKPLYHDTLSLVTLVPKA
HCCCCEEECCCEEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHH
QVNLLTQKVHRRCAGPRKRIGVILLGVGNIGEAWVSLFRRAQSSLCRELEARIELVGLVS
HHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
SSRAYINNAGVHLENWKTEFEEYATDWQYGQLFEALSTLNCDELVALDISASASLTLQYP
CCCHHCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECH
EFFARGIHVVSANKLAGSGPLPFYRDLKQQLGNRRLYWRYNASCGAGLPVQHALNDLHNS
HHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCC
GDSVEAVGGIFSGTLCWLFEHYDGQRPFSDLVIEARGLGITEPDPRDDLSGRDMQRKLLI
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHH
LAREIGLDLELDDIQVESPVPPHLAELTLDEFLARIDELDAPMADALAAAAAEGKVLRYI
HHHHHCCCEEECCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHH
AALDRAGGQLMARVGLQWVDKAHPYANLTPGDNVFVIRSTFYQGNPLIIRGPGAGREVTA
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCCCCHHHH
AAVQSDLVQICRDLLQD
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARCHLHKFGGSSLADADCYRRVAHILLTQGHSDDLVVVSAAGKTTNFLYKLLSLRDAGE
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCHHH
LWQEELQVLISYQQNLIEQLLSAEQARYLRERLSTDKAQLVSLLSLATLNDYQTSHVVSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHH
GERWSARLMAALLRESGVAASHVDACSILVADEGAVPNIREQESREKVAAMLAEHENERI
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEE
VITGFICANPAGETLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
EEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHH
LKSMSLAEADRLARLGSPVLHSRTLQPLFNTQVSMAVRSSYAPHTDFTLVAPTSSQASAP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
VVTNLSEVALFSLTTQADTDALLATLSASGLTPLASWQGSRGGLELAYTPENLKQVSAFL
HHHCHHHHHEEEEECCCCHHHHHHHHHCCCCCCHHHCCCCCCCEEEEECHHHHHHHHHHH
KANAEAYGLGDVSISNDFGLVALVSADAVLYRKSFARLLSREAKPLYHDTLSLVTLVPKA
HCCCCEEECCCEEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHH
QVNLLTQKVHRRCAGPRKRIGVILLGVGNIGEAWVSLFRRAQSSLCRELEARIELVGLVS
HHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
SSRAYINNAGVHLENWKTEFEEYATDWQYGQLFEALSTLNCDELVALDISASASLTLQYP
CCCHHCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECH
EFFARGIHVVSANKLAGSGPLPFYRDLKQQLGNRRLYWRYNASCGAGLPVQHALNDLHNS
HHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCC
GDSVEAVGGIFSGTLCWLFEHYDGQRPFSDLVIEARGLGITEPDPRDDLSGRDMQRKLLI
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHH
LAREIGLDLELDDIQVESPVPPHLAELTLDEFLARIDELDAPMADALAAAAAEGKVLRYI
HHHHHCCCEEECCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHH
AALDRAGGQLMARVGLQWVDKAHPYANLTPGDNVFVIRSTFYQGNPLIIRGPGAGREVTA
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCCCCHHHH
AAVQSDLVQICRDLLQD
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]