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The map label for this gene is dusA [H]

Identifier: 119776187

GI number: 119776187

Start: 3630940

End: 3631920

Strand: Reverse

Name: dusA [H]

Synonym: Sama_3055

Alternate gene names: 119776187

Gene position: 3631920-3630940 (Counterclockwise)

Preceding gene: 119776189

Following gene: 119776186

Centisome position: 84.34

GC content: 53.41

Gene sequence:

>981_bases
ATGCCATCGCCACTCAACCGCACCTTCTCTGTCGCTCCCATGCTGGATTGGACCGACAGACACTACCGCTATTTTGCACG
CCTGATGTCCAAAGAGGCGCTGCTTTATACCGAAATGGTGACGACGGGCGCAATTCTCCATGGCAAAGGCGATTATCTGG
CCTACAACCAAGAAGAACACCCCCTGGCATTGCAGCTGGGCGGTTCGGACCCGAAGGATTTGGCGACCTGTGCCAAGCTC
GCTGAGGCCCGAGGTTATGATGAAGTGAATATCAACGTAGGTTGCCCTTCGGATCGGGTACAGAATGGTCGCTTTGGCGC
CTGTTTGATGGCCGAGCCTAAGTTGGTTGCTGAGTGTGTAGACGCTATGCGCCAGCAGGTGTCTGTACCTGTCACAGTGA
AGACCCGAATTGGCATTGATGAGCAGGACTCTTACGGTTTTCTCACCGACTTTGTCGAGACCGTCTCAGCGGCTGGTTGC
GATACCTTTATTATTCATGCCCGTAAGGCCTGGCTGCAGGGATTGAGTCCGAAAGAGAACCGTGAAATTCCTCCCCTGGA
TTACGAGCGGGTGTATCAGCTGAAACGGGACTACCCCCAACTTTCCATCAGTATCAATGGTGGCGTGACAAGTATAGAGC
AGGCTAAAACCCATTTGGCACAGCTCGATGGTGTCATGGTGGGTCGTGAAGCGTATCAGAACCCCTATATTTTAGCCTCG
GTGGACACCGAGCTTTGCGGCAAGCCCGCTGTTGTAAGCAGCCGTGAGGAGGTTATCGGGAAGTTGCTTCCTTATATCGG
GAAACACCTTGCCGATGGCGGACGCCTGAACCATATCAGTCGGCACATCATTGGCTTGTATCAGGGCTTGCCTGGCTCTC
GCGCCTGGAGGCGACACCTCAGTGAAAATGCCCATAAGCCGGGAGCAGGTATCGAAGTGATTGAAAATGCCATAAAAGCC
ATGGAGCTTACCACCAATTAA

Upstream 100 bases:

>100_bases
CTTTTACCCCGACTGACAGCGGCTATTATGGGTGTCGTTTTTTATTGTGCGAGTGTAAAATAGCCGCCCTTTTACGCAGT
CTACAGGATGGGGAAAGCTC

Downstream 100 bases:

>100_bases
GACTCTCCAGGGCGTCATTTGGTGAAATTAACCACAACTGTGGTTAATTTCACCTGTCTCCAATCTTACCGCCCACCTCA
GACCTCATCCTCTTTTTAGC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPSPLNRTFSVAPMLDWTDRHYRYFARLMSKEALLYTEMVTTGAILHGKGDYLAYNQEEHPLALQLGGSDPKDLATCAKL
AEARGYDEVNINVGCPSDRVQNGRFGACLMAEPKLVAECVDAMRQQVSVPVTVKTRIGIDEQDSYGFLTDFVETVSAAGC
DTFIIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPQLSISINGGVTSIEQAKTHLAQLDGVMVGREAYQNPYILAS
VDTELCGKPAVVSSREEVIGKLLPYIGKHLADGGRLNHISRHIIGLYQGLPGSRAWRRHLSENAHKPGAGIEVIENAIKA
MELTTN

Sequences:

>Translated_326_residues
MPSPLNRTFSVAPMLDWTDRHYRYFARLMSKEALLYTEMVTTGAILHGKGDYLAYNQEEHPLALQLGGSDPKDLATCAKL
AEARGYDEVNINVGCPSDRVQNGRFGACLMAEPKLVAECVDAMRQQVSVPVTVKTRIGIDEQDSYGFLTDFVETVSAAGC
DTFIIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPQLSISINGGVTSIEQAKTHLAQLDGVMVGREAYQNPYILAS
VDTELCGKPAVVSSREEVIGKLLPYIGKHLADGGRLNHISRHIIGLYQGLPGSRAWRRHLSENAHKPGAGIEVIENAIKA
MELTTN
>Mature_325_residues
PSPLNRTFSVAPMLDWTDRHYRYFARLMSKEALLYTEMVTTGAILHGKGDYLAYNQEEHPLALQLGGSDPKDLATCAKLA
EARGYDEVNINVGCPSDRVQNGRFGACLMAEPKLVAECVDAMRQQVSVPVTVKTRIGIDEQDSYGFLTDFVETVSAAGCD
TFIIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPQLSISINGGVTSIEQAKTHLAQLDGVMVGREAYQNPYILASV
DTELCGKPAVVSSREEVIGKLLPYIGKHLADGGRLNHISRHIIGLYQGLPGSRAWRRHLSENAHKPGAGIEVIENAIKAM
ELTTN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=27.1186440677966, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=70.8333333333333, Blast_Score=473, Evalue=1e-135,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=25.6172839506173, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788462, Length=176, Percent_Identity=28.9772727272727, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=234, Percent_Identity=24.7863247863248, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=25.6302521008403, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24580595, Length=242, Percent_Identity=26.8595041322314, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19920448, Length=242, Percent_Identity=26.8595041322314, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36053; Mature: 35921

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPLNRTFSVAPMLDWTDRHYRYFARLMSKEALLYTEMVTTGAILHGKGDYLAYNQEEH
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCC
PLALQLGGSDPKDLATCAKLAEARGYDEVNINVGCPSDRVQNGRFGACLMAEPKLVAECV
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCHHHHHHHH
DAMRQQVSVPVTVKTRIGIDEQDSYGFLTDFVETVSAAGCDTFIIHARKAWLQGLSPKEN
HHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
REIPPLDYERVYQLKRDYPQLSISINGGVTSIEQAKTHLAQLDGVMVGREAYQNPYILAS
CCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEEE
VDTELCGKPAVVSSREEVIGKLLPYIGKHLADGGRLNHISRHIIGLYQGLPGSRAWRRHL
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
SENAHKPGAGIEVIENAIKAMELTTN
HHCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSPLNRTFSVAPMLDWTDRHYRYFARLMSKEALLYTEMVTTGAILHGKGDYLAYNQEEH
CCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCC
PLALQLGGSDPKDLATCAKLAEARGYDEVNINVGCPSDRVQNGRFGACLMAEPKLVAECV
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCHHHHHHHH
DAMRQQVSVPVTVKTRIGIDEQDSYGFLTDFVETVSAAGCDTFIIHARKAWLQGLSPKEN
HHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
REIPPLDYERVYQLKRDYPQLSISINGGVTSIEQAKTHLAQLDGVMVGREAYQNPYILAS
CCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEEE
VDTELCGKPAVVSSREEVIGKLLPYIGKHLADGGRLNHISRHIIGLYQGLPGSRAWRRHL
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
SENAHKPGAGIEVIENAIKAMELTTN
HHCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]