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The map label for this gene is hflK [H]

Identifier: 119776155

GI number: 119776155

Start: 3597186

End: 3598319

Strand: Reverse

Name: hflK [H]

Synonym: Sama_3023

Alternate gene names: 119776155

Gene position: 3598319-3597186 (Counterclockwise)

Preceding gene: 119776156

Following gene: 119776154

Centisome position: 83.56

GC content: 54.94

Gene sequence:

>1134_bases
ATGGCTTGGAACGAGCCCGGTAACAAGGGGCAGGACCCTTGGGGAAACAAAGGCGGCAACGATAAAGGGCCGCCGGATCT
GGACGAAGTCTTTCGCAATATTTCCAAACGTTTTGGCGGCAAGGGCAATGGCCTGGGCTTTGGTGGCCTGGGGTTAATTA
TCGCGCTTGGTGCTGCTGTATGGTTCCTCTCCGGTTTTTACACCATCAAAACCGCAGAGCGCGGTGTACATCTGCGCTTT
GGTGAGTACATTGGTGAAGTTGGTCCCGGTCTGCGCTGGAAAGCCACCTTTATTGATGAAGTGTATCCGGTCGATGTGGA
AGCCCGTCGTACCATCCCGGCTTCTGGCAGCATTCTGACTTCCGATGAGAACGTGGTGCTGGTCGAGCTGGCTGTGCAGT
ACAAGGTGACTGATGCATACCAGTACATGTTCAGTGCTGTGGATGCCAATTCCTCCCTGCGTGAAGCCACCGACAGCGCA
CTGCGTTATGTGGTGGGCCACTCTAAGATGGATGACATTCTCACCACTGGCCGCGACAAAATCCGTACCGATACCTGGGC
CGAGCTTGAGCGTATTATTGAGCCTTACAAGCTGGGTCTGACCATCATGGACGTTAACTTCCTGCCGGCACGTCCGCCGG
AAGAAGTGAAAGATGCCTTCGATGATGCCATTGCCGCACAGGAAGATGAGCAGCGCTTTATCCGTGAAGCTGAAGCTTAT
CAGCGTGAAGTTGAGCCCCGTGCCCGTGGTCAGGAACAGCGTATTGCTGAAGATGCCCGCGCCTACAAAGAGCAGGTGGT
TCAGCAGGCACAGGGTGCCGTTGCACGCTTTGAAAAGCTGCTGCCTGAATACAAGGCAGCGCCTGAGGTGACTCGCCAGC
GCATGTATATCGAGGCCATGGAAGAAGTGCTTTCAGGTAACAACAAGGTGCTTATCGATGCCAAGAACAACGGTAATCTT
CTGTATCTGCCATTGGATAAGATGATCACGCCTTCTGCTGCTGTGCCCACGTCAGACGACAGTGGTATCACGTCAACCAC
GCATCTGGATACCGGCGCCTTACAGCGCAGTACCGGACTGCCGGTAAGCGGCCGTCCTTCCCGTGAAGATCGTGTTCGCC
AGGGGAGGGAGTAA

Upstream 100 bases:

>100_bases
GCTAAACAGAGTCAAGGTGAGTTGGAAACCTTTATTGTCGATACAGCGACTGATAGGGTAGTTTGTTAACTATTAATATC
ATCCACTTATGGAGTATTAA

Downstream 100 bases:

>100_bases
GTTATGAGTCGTTTAATATTGGTTATTCTGGTGGTGGTGATTGCTGTGATGAGTTCGTCTCTCATGGTAGTCAACGAGGG
TGAACGTGCCATCGTGTCGC

Product: hflK protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAWNEPGNKGQDPWGNKGGNDKGPPDLDEVFRNISKRFGGKGNGLGFGGLGLIIALGAAVWFLSGFYTIKTAERGVHLRF
GEYIGEVGPGLRWKATFIDEVYPVDVEARRTIPASGSILTSDENVVLVELAVQYKVTDAYQYMFSAVDANSSLREATDSA
LRYVVGHSKMDDILTTGRDKIRTDTWAELERIIEPYKLGLTIMDVNFLPARPPEEVKDAFDDAIAAQEDEQRFIREAEAY
QREVEPRARGQEQRIAEDARAYKEQVVQQAQGAVARFEKLLPEYKAAPEVTRQRMYIEAMEEVLSGNNKVLIDAKNNGNL
LYLPLDKMITPSAAVPTSDDSGITSTTHLDTGALQRSTGLPVSGRPSREDRVRQGRE

Sequences:

>Translated_377_residues
MAWNEPGNKGQDPWGNKGGNDKGPPDLDEVFRNISKRFGGKGNGLGFGGLGLIIALGAAVWFLSGFYTIKTAERGVHLRF
GEYIGEVGPGLRWKATFIDEVYPVDVEARRTIPASGSILTSDENVVLVELAVQYKVTDAYQYMFSAVDANSSLREATDSA
LRYVVGHSKMDDILTTGRDKIRTDTWAELERIIEPYKLGLTIMDVNFLPARPPEEVKDAFDDAIAAQEDEQRFIREAEAY
QREVEPRARGQEQRIAEDARAYKEQVVQQAQGAVARFEKLLPEYKAAPEVTRQRMYIEAMEEVLSGNNKVLIDAKNNGNL
LYLPLDKMITPSAAVPTSDDSGITSTTHLDTGALQRSTGLPVSGRPSREDRVRQGRE
>Mature_376_residues
AWNEPGNKGQDPWGNKGGNDKGPPDLDEVFRNISKRFGGKGNGLGFGGLGLIIALGAAVWFLSGFYTIKTAERGVHLRFG
EYIGEVGPGLRWKATFIDEVYPVDVEARRTIPASGSILTSDENVVLVELAVQYKVTDAYQYMFSAVDANSSLREATDSAL
RYVVGHSKMDDILTTGRDKIRTDTWAELERIIEPYKLGLTIMDVNFLPARPPEEVKDAFDDAIAAQEDEQRFIREAEAYQ
REVEPRARGQEQRIAEDARAYKEQVVQQAQGAVARFEKLLPEYKAAPEVTRQRMYIEAMEEVLSGNNKVLIDAKNNGNLL
YLPLDKMITPSAAVPTSDDSGITSTTHLDTGALQRSTGLPVSGRPSREDRVRQGRE

Specific function: HflC and HflK govern the stability of phage lambda cII protein and have been proposed to encode or regulate a cII- specific protease [H]

COG id: COG0330

COG function: function code O; Membrane protease subunits, stomatin/prohibitin homologs

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the band 7/mec-2 family. HflK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790616, Length=380, Percent_Identity=55, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001107
- InterPro:   IPR010201
- InterPro:   IPR020980
- InterPro:   IPR001972 [H]

Pfam domain/function: PF01145 Band_7; PF12221 HflK_N [H]

EC number: NA

Molecular weight: Translated: 41801; Mature: 41670

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWNEPGNKGQDPWGNKGGNDKGPPDLDEVFRNISKRFGGKGNGLGFGGLGLIIALGAAV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
WFLSGFYTIKTAERGVHLRFGEYIGEVGPGLRWKATFIDEVYPVDVEARRTIPASGSILT
HHHHCCEEEEECCCCEEEEHHHHHHHHCCCCEEEEEHHCCCCCCCCHHCCCCCCCCCEEE
SDENVVLVELAVQYKVTDAYQYMFSAVDANSSLREATDSALRYVVGHSKMDDILTTGRDK
CCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHH
IRTDTWAELERIIEPYKLGLTIMDVNFLPARPPEEVKDAFDDAIAAQEDEQRFIREAEAY
HCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
QREVEPRARGQEQRIAEDARAYKEQVVQQAQGAVARFEKLLPEYKAAPEVTRQRMYIEAM
HHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EEVLSGNNKVLIDAKNNGNLLYLPLDKMITPSAAVPTSDDSGITSTTHLDTGALQRSTGL
HHHHCCCCEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHCCCC
PVSGRPSREDRVRQGRE
CCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
AWNEPGNKGQDPWGNKGGNDKGPPDLDEVFRNISKRFGGKGNGLGFGGLGLIIALGAAV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
WFLSGFYTIKTAERGVHLRFGEYIGEVGPGLRWKATFIDEVYPVDVEARRTIPASGSILT
HHHHCCEEEEECCCCEEEEHHHHHHHHCCCCEEEEEHHCCCCCCCCHHCCCCCCCCCEEE
SDENVVLVELAVQYKVTDAYQYMFSAVDANSSLREATDSALRYVVGHSKMDDILTTGRDK
CCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHH
IRTDTWAELERIIEPYKLGLTIMDVNFLPARPPEEVKDAFDDAIAAQEDEQRFIREAEAY
HCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
QREVEPRARGQEQRIAEDARAYKEQVVQQAQGAVARFEKLLPEYKAAPEVTRQRMYIEAM
HHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EEVLSGNNKVLIDAKNNGNLLYLPLDKMITPSAAVPTSDDSGITSTTHLDTGALQRSTGL
HHHHCCCCEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHCCCC
PVSGRPSREDRVRQGRE
CCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]