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The map label for this gene is mlhB [H]

Identifier: 119776144

GI number: 119776144

Start: 3585475

End: 3586386

Strand: Reverse

Name: mlhB [H]

Synonym: Sama_3012

Alternate gene names: 119776144

Gene position: 3586386-3585475 (Counterclockwise)

Preceding gene: 119776145

Following gene: 119776143

Centisome position: 83.29

GC content: 57.13

Gene sequence:

>912_bases
ATGGCTAGTTGGCAGGCAAAGGTGCTTAACAGTGTGCTTTCTTACATTGCCAAACCCGGAATGCACGCCCGGGGCGCCCG
TTTGTCACTGGCCGATATTCGCAGGCGGCTTGAAGCCCTGGACAGCCGCTATCTTAGCTGGCCCAAAGGGCTTATCAGTG
ACAATTTGCCCCTCTCCCACAGCGCCTTAATCCATAATCATCTGGAATCCGCTGCGGCACAACGCCCCCGGCTCGCCATC
TTTTACGTGCGTGGAGGGGGATTTTGTTTTAAAACCCCCAACGCCCATGGCCGCTTTATCGCAGATCTGTGTCGTGAGCT
GGATGCCGATGCCTATGTCCCCGATTATCGGCTGGCACCAGAACACCCGTTTCCTGCCCCCCTGGAAGATGTGCTGGACG
GGTATCAGCAATTTCTGTCGCTTTGGTCAGACCCCTTTGTGGTGATGGGTGACAGCGCCGGTGGTAATCTTGCCATGGCT
TTGCTGCTGCAAGTCAAAGATATGGGGCTCAGATTGCCCGAATGTGCGGTGCTGCTCAGCCCCGCGCTGGACCTTGGGAT
CACAGGTGACAGTGAACGGCTGCTCAGTGCAGACGATCCCTTCTTCAGTATCGACTCCCTGCTCAGGCTTAGGGGGGCCT
ATCTTCAGGGCGCCAATCCCATGGATGTCCGGGCAAGTCCATTGCATGGTGATTTACGGGGCCTTCCGTCCATGCTGCTG
CTTGCGGGCACGCGGGAGCTCCTTTTGGAGGATTCACAGCGCCTTGCACAAAGGCTGAAGGAAGCGGGAACCCATGTGGA
ACATCACTTTCTGGCGCACATGCCCCATGTGTTTCCGCTGTTTGAAATGCTGCCCGAAGCCAGGGAAGCGAGGGAACTCA
TCCTCGAATTTATCCGGCGGCGCCTGATGTGA

Upstream 100 bases:

>100_bases
CTTGAAGGCGCAAAATTACCGCAGAATGGAACTCTTCTGAAACGCGTTGTTCGGGGCGTTAGCCGAACATCCGGCAAAGC
AGTACAAGCAGGAGCTCAGT

Downstream 100 bases:

>100_bases
ATAATATGTCTATTTATCCAATATTCTATGCATTGTCTGAAAATTTGCCGGTAAATAAGATTTTTTCTGACTAAATATGC
CATTATCCCCCGCATATAGC

Product: esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MASWQAKVLNSVLSYIAKPGMHARGARLSLADIRRRLEALDSRYLSWPKGLISDNLPLSHSALIHNHLESAAAQRPRLAI
FYVRGGGFCFKTPNAHGRFIADLCRELDADAYVPDYRLAPEHPFPAPLEDVLDGYQQFLSLWSDPFVVMGDSAGGNLAMA
LLLQVKDMGLRLPECAVLLSPALDLGITGDSERLLSADDPFFSIDSLLRLRGAYLQGANPMDVRASPLHGDLRGLPSMLL
LAGTRELLLEDSQRLAQRLKEAGTHVEHHFLAHMPHVFPLFEMLPEAREARELILEFIRRRLM

Sequences:

>Translated_303_residues
MASWQAKVLNSVLSYIAKPGMHARGARLSLADIRRRLEALDSRYLSWPKGLISDNLPLSHSALIHNHLESAAAQRPRLAI
FYVRGGGFCFKTPNAHGRFIADLCRELDADAYVPDYRLAPEHPFPAPLEDVLDGYQQFLSLWSDPFVVMGDSAGGNLAMA
LLLQVKDMGLRLPECAVLLSPALDLGITGDSERLLSADDPFFSIDSLLRLRGAYLQGANPMDVRASPLHGDLRGLPSMLL
LAGTRELLLEDSQRLAQRLKEAGTHVEHHFLAHMPHVFPLFEMLPEAREARELILEFIRRRLM
>Mature_302_residues
ASWQAKVLNSVLSYIAKPGMHARGARLSLADIRRRLEALDSRYLSWPKGLISDNLPLSHSALIHNHLESAAAQRPRLAIF
YVRGGGFCFKTPNAHGRFIADLCRELDADAYVPDYRLAPEHPFPAPLEDVLDGYQQFLSLWSDPFVVMGDSAGGNLAMAL
LLQVKDMGLRLPECAVLLSPALDLGITGDSERLLSADDPFFSIDSLLRLRGAYLQGANPMDVRASPLHGDLRGLPSMLLL
AGTRELLLEDSQRLAQRLKEAGTHVEHHFLAHMPHVFPLFEMLPEAREARELILEFIRRRLM

Specific function: Lactone hydrolase that is required for the degradation of monocyclic monoterpenes such as menthol and carvone. Catalyzes the ring opening of lactones formed during degradation of monocyclic monoterpenes. Can hydrolyze epsilon-caprolactone, the lactone der

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=185, Percent_Identity=33.5135135135135, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533410, Length=164, Percent_Identity=30.4878048780488, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=164, Percent_Identity=30.4878048780488, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24656084, Length=141, Percent_Identity=30.4964539007092, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656076, Length=141, Percent_Identity=30.4964539007092, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20130169, Length=141, Percent_Identity=30.4964539007092, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.83 [H]

Molecular weight: Translated: 33788; Mature: 33656

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASWQAKVLNSVLSYIAKPGMHARGARLSLADIRRRLEALDSRYLSWPKGLISDNLPLSH
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCH
SALIHNHLESAAAQRPRLAIFYVRGGGFCFKTPNAHGRFIADLCRELDADAYVPDYRLAP
HHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCC
EHPFPAPLEDVLDGYQQFLSLWSDPFVVMGDSAGGNLAMALLLQVKDMGLRLPECAVLLS
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
PALDLGITGDSERLLSADDPFFSIDSLLRLRGAYLQGANPMDVRASPLHGDLRGLPSMLL
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LAGTRELLLEDSQRLAQRLKEAGTHVEHHFLAHMPHVFPLFEMLPEAREARELILEFIRR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLM
HCC
>Mature Secondary Structure 
ASWQAKVLNSVLSYIAKPGMHARGARLSLADIRRRLEALDSRYLSWPKGLISDNLPLSH
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCH
SALIHNHLESAAAQRPRLAIFYVRGGGFCFKTPNAHGRFIADLCRELDADAYVPDYRLAP
HHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCC
EHPFPAPLEDVLDGYQQFLSLWSDPFVVMGDSAGGNLAMALLLQVKDMGLRLPECAVLLS
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
PALDLGITGDSERLLSADDPFFSIDSLLRLRGAYLQGANPMDVRASPLHGDLRGLPSMLL
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LAGTRELLLEDSQRLAQRLKEAGTHVEHHFLAHMPHVFPLFEMLPEAREARELILEFIRR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLM
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA