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The map label for this gene is ycgF [H]

Identifier: 119775863

GI number: 119775863

Start: 3265052

End: 3265831

Strand: Direct

Name: ycgF [H]

Synonym: Sama_2731

Alternate gene names: 119775863

Gene position: 3265052-3265831 (Clockwise)

Preceding gene: 119775862

Following gene: 119775868

Centisome position: 75.82

GC content: 48.72

Gene sequence:

>780_bases
GTGGACTTTCCTGAAGAAATCAAACAGCAAACCTGTGCCGACTGCCTGAGCGGTGTCTCACTTGGCTTTCGTATCCGCAT
GGCATTTCAGCCCATCATCGATTGGCAAACCCGCAACATCTTCGCCTACGAGGCATTGGTGAGAGGGCCCGAAGGACAAG
GCGCTGGCTGGGTGTTTGAGCACATCAACGACACCAACAAGTACTATTTTGATCAGACATGCAGAATCACCGCCATCAAA
ACCGCTGCGGAGCTTGGATGCGACACCTTTTTATCCATCAACTTTTTGCCTAACGCCGTCTACAACCCGCAAACCTGCAT
CAAGGCAACCATAGAGGCAGCCCACCTTTACAACTTCGATTTGAAAAAACTCATCTTTGAAGTGACTGAAGGGGAAAATA
TTATCGACAAGGCGCACTTGCGGAACATTTTCCAAAGCTATGCCAAATTCGGCTTTTTAACTGCTATCGATGACTATGGT
GCGGGCTATACCCACATTAGTTGGCTGTCAGAGCTGAAACCCCATGTGCTCAAATTGGACATGTCGCTCATCCGTGACAT
AGACAAAGACCCACTCAAACAGGCACAAATCAGGGAAATCAAAGCCCAGTGTGACCTGCAATGCACTCAACTTCTCGCTG
AAGGCATTGAAACCCATGAAGAACTCAGCTGTCTGACCGCGATGGGTATTCGCTATTTACAAGGGTATCTGTTCGCCCCG
CCCACGCTTGAACACCTCACCAAGGAAAGCGACATAGAACTACTTCAGGCAATTGATTGA

Upstream 100 bases:

>100_bases
TGTGCAATCCAGACCTGCACCCATAAGTGCTTGGCATTCTCACCCGCTGCATCTACAGTAATCTCACACCCGTTATTTTG
CGGACCCTTGAGACTACAAC

Downstream 100 bases:

>100_bases
TAAGTGGATTATCTTTAACGCCCTGGGATACGACTCAAAGACAGTACCGGATAAAAAGCCGCTTCTGTTGCCTTAAATAA
GACGATGCCATGGCAAGAGC

Product: putative diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDFPEEIKQQTCADCLSGVSLGFRIRMAFQPIIDWQTRNIFAYEALVRGPEGQGAGWVFEHINDTNKYYFDQTCRITAIK
TAAELGCDTFLSINFLPNAVYNPQTCIKATIEAAHLYNFDLKKLIFEVTEGENIIDKAHLRNIFQSYAKFGFLTAIDDYG
AGYTHISWLSELKPHVLKLDMSLIRDIDKDPLKQAQIREIKAQCDLQCTQLLAEGIETHEELSCLTAMGIRYLQGYLFAP
PTLEHLTKESDIELLQAID

Sequences:

>Translated_259_residues
MDFPEEIKQQTCADCLSGVSLGFRIRMAFQPIIDWQTRNIFAYEALVRGPEGQGAGWVFEHINDTNKYYFDQTCRITAIK
TAAELGCDTFLSINFLPNAVYNPQTCIKATIEAAHLYNFDLKKLIFEVTEGENIIDKAHLRNIFQSYAKFGFLTAIDDYG
AGYTHISWLSELKPHVLKLDMSLIRDIDKDPLKQAQIREIKAQCDLQCTQLLAEGIETHEELSCLTAMGIRYLQGYLFAP
PTLEHLTKESDIELLQAID
>Mature_259_residues
MDFPEEIKQQTCADCLSGVSLGFRIRMAFQPIIDWQTRNIFAYEALVRGPEGQGAGWVFEHINDTNKYYFDQTCRITAIK
TAAELGCDTFLSINFLPNAVYNPQTCIKATIEAAHLYNFDLKKLIFEVTEGENIIDKAHLRNIFQSYAKFGFLTAIDDYG
AGYTHISWLSELKPHVLKLDMSLIRDIDKDPLKQAQIREIKAQCDLQCTQLLAEGIETHEELSCLTAMGIRYLQGYLFAP
PTLEHLTKESDIELLQAID

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=218, Percent_Identity=31.1926605504587, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787541, Length=138, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790496, Length=231, Percent_Identity=28.1385281385281, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082096, Length=176, Percent_Identity=28.9772727272727, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081980, Length=121, Percent_Identity=35.5371900826446, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI226510982, Length=238, Percent_Identity=25.6302521008403, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788849, Length=185, Percent_Identity=27.5675675675676, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786507, Length=123, Percent_Identity=30.0813008130081, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081845, Length=229, Percent_Identity=25.764192139738, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 29469; Mature: 29469

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPEEIKQQTCADCLSGVSLGFRIRMAFQPIIDWQTRNIFAYEALVRGPEGQGAGWVFE
CCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHH
HINDTNKYYFDQTCRITAIKTAAELGCDTFLSINFLPNAVYNPQTCIKATIEAAHLYNFD
CCCCCCCEEECCCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCHH
LKKLIFEVTEGENIIDKAHLRNIFQSYAKFGFLTAIDDYGAGYTHISWLSELKPHVLKLD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHH
MSLIRDIDKDPLKQAQIREIKAQCDLQCTQLLAEGIETHEELSCLTAMGIRYLQGYLFAP
HHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTLEHLTKESDIELLQAID
CCHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MDFPEEIKQQTCADCLSGVSLGFRIRMAFQPIIDWQTRNIFAYEALVRGPEGQGAGWVFE
CCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHH
HINDTNKYYFDQTCRITAIKTAAELGCDTFLSINFLPNAVYNPQTCIKATIEAAHLYNFD
CCCCCCCEEECCCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCHH
LKKLIFEVTEGENIIDKAHLRNIFQSYAKFGFLTAIDDYGAGYTHISWLSELKPHVLKLD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHH
MSLIRDIDKDPLKQAQIREIKAQCDLQCTQLLAEGIETHEELSCLTAMGIRYLQGYLFAP
HHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTLEHLTKESDIELLQAID
CCHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]