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The map label for this gene is lipB

Identifier: 119775726

GI number: 119775726

Start: 3101908

End: 3102558

Strand: Direct

Name: lipB

Synonym: Sama_2594

Alternate gene names: 119775726

Gene position: 3101908-3102558 (Clockwise)

Preceding gene: 119775725

Following gene: 119775727

Centisome position: 72.03

GC content: 53.92

Gene sequence:

>651_bases
TTGCAAGCCAAGACTCTGCATATCAGACATCTGGGTAATCAGGATTATGAAACTGTCTGGCACGCCATGCAGCATTACAC
CGATAACCGCGATGAAAACAGCCCGGACGAACTCTGGTTGGTTGAACATCCTCCGGTATTTACCCAGGGGCAAGCCGGTA
AAGCCGAGCACATCCTCAATCCCGGCGACATCCCTGTTATCCAGGTAGACCGCGGTGGTCAGGTGACGTATCACGGCCCG
GGTCAGTTGGTTGCATACCCCCTGCTGGACATCAAACGCCTTAAAATCGGTGTTCGTCAGCTGGTTACCCATATAGAGCA
GAGCATAGTCGACATGCTGAAGCCCTACGGCGTACAAGCCTACGCTAAAGCCGATGCCCCCGGAGTCTATGTTGACGAGC
GTAAAGTCGCCTCCCTTGGCCTGCGAATCCGCCGTGGTTGTTCTTTCCACGGTCTGGCACTGAACGTCGACATGGATATT
TCCCCCTTCCAGCGCATCAACCCCTGTGGCTATGCCGGCCTGGAAATGGTTCAGTGCAAAGCACTGGGCGGCCCCAATAC
CGTAAACGAAGCAGGTGAAAAACTCACCCTCACCTTTAGCCAGCTTCTTGGCTATGAGCATCTGGTCCATCACCAAGGAT
TAGCAGAATAA

Upstream 100 bases:

>100_bases
CTTTAATTATTCCGGCGCGCCCTGTAAGCGCTTCTGTGTTACATTTCGGCATCCGCGGCGTATAATGCCGCCACGCTGAA
CCTGAGGGGAGAGGTAGCCC

Downstream 100 bases:

>100_bases
AATGAACAGACCCGAAAGATTGCAGCCGGGCGTCAAACTGCGTGACGCAGACAAAGTCGCCCGAATTCCGGTAAAAATCA
TGCCATCCGAGCGTGAGACC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MQAKTLHIRHLGNQDYETVWHAMQHYTDNRDENSPDELWLVEHPPVFTQGQAGKAEHILNPGDIPVIQVDRGGQVTYHGP
GQLVAYPLLDIKRLKIGVRQLVTHIEQSIVDMLKPYGVQAYAKADAPGVYVDERKVASLGLRIRRGCSFHGLALNVDMDI
SPFQRINPCGYAGLEMVQCKALGGPNTVNEAGEKLTLTFSQLLGYEHLVHHQGLAE

Sequences:

>Translated_216_residues
MQAKTLHIRHLGNQDYETVWHAMQHYTDNRDENSPDELWLVEHPPVFTQGQAGKAEHILNPGDIPVIQVDRGGQVTYHGP
GQLVAYPLLDIKRLKIGVRQLVTHIEQSIVDMLKPYGVQAYAKADAPGVYVDERKVASLGLRIRRGCSFHGLALNVDMDI
SPFQRINPCGYAGLEMVQCKALGGPNTVNEAGEKLTLTFSQLLGYEHLVHHQGLAE
>Mature_216_residues
MQAKTLHIRHLGNQDYETVWHAMQHYTDNRDENSPDELWLVEHPPVFTQGQAGKAEHILNPGDIPVIQVDRGGQVTYHGP
GQLVAYPLLDIKRLKIGVRQLVTHIEQSIVDMLKPYGVQAYAKADAPGVYVDERKVASLGLRIRRGCSFHGLALNVDMDI
SPFQRINPCGYAGLEMVQCKALGGPNTVNEAGEKLTLTFSQLLGYEHLVHHQGLAE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=106, Percent_Identity=42.4528301886792, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI87081767, Length=203, Percent_Identity=61.576354679803, Blast_Score=260, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6323268, Length=117, Percent_Identity=40.1709401709402, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355483, Length=186, Percent_Identity=31.7204301075269, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_SHEAM (A1S8U0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928466.1
- ProteinModelPortal:   A1S8U0
- SMR:   A1S8U0
- STRING:   A1S8U0
- GeneID:   4604841
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2594
- NMPDR:   fig|326297.7.peg.2432
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   A1S8U0
- ProtClustDB:   PRK14342
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24034; Mature: 24034

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 169-169

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAKTLHIRHLGNQDYETVWHAMQHYTDNRDENSPDELWLVEHPPVFTQGQAGKAEHILN
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCC
PGDIPVIQVDRGGQVTYHGPGQLVAYPLLDIKRLKIGVRQLVTHIEQSIVDMLKPYGVQA
CCCCCEEEECCCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YAKADAPGVYVDERKVASLGLRIRRGCSFHGLALNVDMDISPFQRINPCGYAGLEMVQCK
EECCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCCCCCCEEEEEE
ALGGPNTVNEAGEKLTLTFSQLLGYEHLVHHQGLAE
ECCCCCCHHHCCCEEEEEHHHHHCHHHHHHHCCCCC
>Mature Secondary Structure
MQAKTLHIRHLGNQDYETVWHAMQHYTDNRDENSPDELWLVEHPPVFTQGQAGKAEHILN
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCC
PGDIPVIQVDRGGQVTYHGPGQLVAYPLLDIKRLKIGVRQLVTHIEQSIVDMLKPYGVQA
CCCCCEEEECCCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YAKADAPGVYVDERKVASLGLRIRRGCSFHGLALNVDMDISPFQRINPCGYAGLEMVQCK
EECCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCCCCCCEEEEEE
ALGGPNTVNEAGEKLTLTFSQLLGYEHLVHHQGLAE
ECCCCCCHHHCCCEEEEEHHHHHCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA