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The map label for this gene is lipA

Identifier: 119775727

GI number: 119775727

Start: 3102560

End: 3103525

Strand: Direct

Name: lipA

Synonym: Sama_2595

Alternate gene names: 119775727

Gene position: 3102560-3103525 (Clockwise)

Preceding gene: 119775726

Following gene: 119775730

Centisome position: 72.05

GC content: 55.38

Gene sequence:

>966_bases
ATGAACAGACCCGAAAGATTGCAGCCGGGCGTCAAACTGCGTGACGCAGACAAAGTCGCCCGAATTCCGGTAAAAATCAT
GCCATCCGAGCGTGAGACCATGCTCAGAAAGCCAGATTGGCTGCGGGTAAAGCTGCCTGCCTCCAATCAGCGCATCACCG
AAATCAAGCAGGCACTGCGCAGCAACGGCCTTCACTCAGTGTGTGAAGAAGCCTCCTGCCCTAACTTGGCAGAATGCTTT
AACCACGGTACCGCCACCTTTATGATTTTGGGTGCCATTTGTACCCGCCGCTGCCCATTCTGCGACGTGGCCCACGGCCG
TCCACTGAAACCTGATGCCGATGAGCCGGTTAAACTGGCAAAGACCATCCGCGATATGAAGCTCAAGTACGTGGTGATCA
CTTCCGTTGACCGCGATGACCTGCGTGACGGCGGTGCCCAGCATTTTGCCGACTGTATCCGTGAAATCCGCGCCCTGAAT
CCGCACATCCAGATTGAAACCCTGGTACCCGATTTCCGCGGCCGTATCGACGTTGCACTGGATATCCTGAGTACCAATCC
ACCGGATGTATTCAACCACAACCTGGAAACTGCACCTGCGCACTATCGCAAGGCTCGCCCGGGCGCCAACTATCAATGGT
CACTGGATCTGCTCAAGCGCTTTAAAGAGCGTCACCCAAACATTCCAACCAAGTCTGGTTTGATGATGGGGCTGGGTGAA
ACCAACGAAGAAATCATTCAGGTGCTCAAAGATCTACGTGCCCACGATGTGAACATGCTGACCCTGGGCCAGTACCTGCA
GCCGTCCAAGTTCCACCTGCCGGTAGAGCGCTACGTGAGCCCACAGGAATTTGATGAGCTGAAAGTGATTGCCGAAGATC
TGGGATTCAGCCACGCCGCCTGTGGCCCGCTGGTGCGCTCAAGCTACCATGCCGACCTCCAGGCACAGGGCAAGGAAGTG
AAATAA

Upstream 100 bases:

>100_bases
CCCAATACCGTAAACGAAGCAGGTGAAAAACTCACCCTCACCTTTAGCCAGCTTCTTGGCTATGAGCATCTGGTCCATCA
CCAAGGATTAGCAGAATAAA

Downstream 100 bases:

>100_bases
GCAGGCTTAGCATTAAAGTCAGGCTTTAATTAAAAACGGCGCACTGGCGCCGTTTTTATTGGGGTAACTTAATCAGCCGT
ATCCAGCCTTAAATCCATCA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNRPERLQPGVKLRDADKVARIPVKIMPSERETMLRKPDWLRVKLPASNQRITEIKQALRSNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDADEPVKLAKTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRALN
PHIQIETLVPDFRGRIDVALDILSTNPPDVFNHNLETAPAHYRKARPGANYQWSLDLLKRFKERHPNIPTKSGLMMGLGE
TNEEIIQVLKDLRAHDVNMLTLGQYLQPSKFHLPVERYVSPQEFDELKVIAEDLGFSHAACGPLVRSSYHADLQAQGKEV
K

Sequences:

>Translated_321_residues
MNRPERLQPGVKLRDADKVARIPVKIMPSERETMLRKPDWLRVKLPASNQRITEIKQALRSNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDADEPVKLAKTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRALN
PHIQIETLVPDFRGRIDVALDILSTNPPDVFNHNLETAPAHYRKARPGANYQWSLDLLKRFKERHPNIPTKSGLMMGLGE
TNEEIIQVLKDLRAHDVNMLTLGQYLQPSKFHLPVERYVSPQEFDELKVIAEDLGFSHAACGPLVRSSYHADLQAQGKEV
K
>Mature_321_residues
MNRPERLQPGVKLRDADKVARIPVKIMPSERETMLRKPDWLRVKLPASNQRITEIKQALRSNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDADEPVKLAKTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRALN
PHIQIETLVPDFRGRIDVALDILSTNPPDVFNHNLETAPAHYRKARPGANYQWSLDLLKRFKERHPNIPTKSGLMMGLGE
TNEEIIQVLKDLRAHDVNMLTLGQYLQPSKFHLPVERYVSPQEFDELKVIAEDLGFSHAACGPLVRSSYHADLQAQGKEV
K

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=45.4225352112676, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=45.1612903225806, Blast_Score=214, Evalue=7e-56,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=75.0778816199377, Blast_Score=516, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI32564533, Length=305, Percent_Identity=40.9836065573771, Blast_Score=227, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=305, Percent_Identity=43.9344262295082, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI221513272, Length=289, Percent_Identity=45.6747404844291, Blast_Score=247, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_SHEAM (A1S8U1)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928467.1
- STRING:   A1S8U1
- GeneID:   4604842
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2595
- NMPDR:   fig|326297.7.peg.2433
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   A1S8U1
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36332; Mature: 36332

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPERLQPGVKLRDADKVARIPVKIMPSERETMLRKPDWLRVKLPASNQRITEIKQALR
CCCCCCCCCCCEECCCCHHHHCCEEECCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHH
SNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPLKPDADEPVKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRALNPHIQIETLVPDFRGRIDVAL
HHHHHCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
DILSTNPPDVFNHNLETAPAHYRKARPGANYQWSLDLLKRFKERHPNIPTKSGLMMGLGE
EEECCCCCHHHCCCCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TNEEIIQVLKDLRAHDVNMLTLGQYLQPSKFHLPVERYVSPQEFDELKVIAEDLGFSHAA
CHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCHHH
CGPLVRSSYHADLQAQGKEVK
HHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure
MNRPERLQPGVKLRDADKVARIPVKIMPSERETMLRKPDWLRVKLPASNQRITEIKQALR
CCCCCCCCCCCEECCCCHHHHCCEEECCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHH
SNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPLKPDADEPVKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRALNPHIQIETLVPDFRGRIDVAL
HHHHHCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
DILSTNPPDVFNHNLETAPAHYRKARPGANYQWSLDLLKRFKERHPNIPTKSGLMMGLGE
EEECCCCCHHHCCCCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TNEEIIQVLKDLRAHDVNMLTLGQYLQPSKFHLPVERYVSPQEFDELKVIAEDLGFSHAA
CHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCHHH
CGPLVRSSYHADLQAQGKEVK
HHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA