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The map label for this gene is zraS [H]

Identifier: 119775435

GI number: 119775435

Start: 2765532

End: 2766605

Strand: Reverse

Name: zraS [H]

Synonym: Sama_2300

Alternate gene names: 119775435

Gene position: 2766605-2765532 (Counterclockwise)

Preceding gene: 119775436

Following gene: 119775434

Centisome position: 64.25

GC content: 55.49

Gene sequence:

>1074_bases
ATGGCCACCAAAGCTTTAGCCAAGATAGCCGACTTTCCGGTACGGGCTCGCCTTCAAGAGGTGAAGTCCAGCGGCGTTGC
ATCTGCCGAGCAAATGGCGCAAATCCTGGAGGCCATGCCCTCTGGCGTGGTGATACTGAATGCCGATGGCATAGTCACGC
TGGCAAATCCCGTTGCGGTTCAGCTGCTGGACCGGCCTTTGGAAGGTGAGCGCTGGCTCAGTGTCATCGCCAGAGCCTTT
TCGCCTCAGGATGACGATGGCCATGAGGTATCGCTGAAGAATGGCCGCAGAGTCAAGCTGGCCATTACGCCACTGGAACC
CGGACAGCTGATTTTGCTGACCGATCTCACAGAAACCCGCCTATTGCAGCGTAACCTTGCTCATCTGCAGCGTCTGTCGG
CCCTTGGCAAAATGGTGGCTACCCTGGCACATCAAATCCGAACTCCCCTGTCGGCTGCACTGCTTTACGCAGCAAACCTT
GCCAGTCCCAAACTCAACCAGGACTCCCGCAGTCGTTTCCAACAGAAGTTGGTGGACAGGCTGAATGAGCTGGAGCGACA
GGTGAACGATATGTTGTTGATGGCAAGGGGCAAAGTGGAAGCGCCCATGTCTTCCATGCCACTGGCAGACATCATTGCCC
CTGTGCTGGCAACCTGTGAGCCCATCGCCACCGATAAAGGCTGTGCAGTACGGTTTCAGGATGAAACCCTTGGCGCCATG
ATGGCGGCCTCCCAGAGCGCACTGAGCTCGGCCATTAATAATCTTTTGATGAACAGCCTCGAAGCAGGCGCCCACCATAT
ACTGCTCCATGGCAGCATACAGGACGGTCGTTTGGTTCTGGCTGTGGTGGACGATGGTAAAGGATTGGAGTCCGGCAGTG
AGCAGCGGGTGATGGAACCCTTTTTTACGACCAAGGCACAGGGTACAGGCCTCGGTTTAGCCGTGGTACAGACAGTCGTG
CGTAACCATGGTGGTGAGCTCAAAATGCAATGTGCTGCCAACCGTGGCTGTAGTGTCATGCTTAAATTCCCCGTGATTTC
AGAAGATTCAGTAAAGGAGGTTAGCCATGCCTGA

Upstream 100 bases:

>100_bases
AAGATTAATTTTACTCAGGTCCCACCCAATTCTCTAAATTTCTACTCTGGCACAGTCCCTGCATTAACCCCGTCAATAAC
GTAATTTTGACGGGGTTTCC

Downstream 100 bases:

>100_bases
AGGCAGACTGCTACTTGTCGAGGACGATGCGTCTCTGCGTGAGGCGCTGCTCGACACCCTGATGCTGGCGCAGTTTGACT
GTGTGGCGCTTCCCAGCGCT

Product: flagellar regulatory protein B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MATKALAKIADFPVRARLQEVKSSGVASAEQMAQILEAMPSGVVILNADGIVTLANPVAVQLLDRPLEGERWLSVIARAF
SPQDDDGHEVSLKNGRRVKLAITPLEPGQLILLTDLTETRLLQRNLAHLQRLSALGKMVATLAHQIRTPLSAALLYAANL
ASPKLNQDSRSRFQQKLVDRLNELERQVNDMLLMARGKVEAPMSSMPLADIIAPVLATCEPIATDKGCAVRFQDETLGAM
MAASQSALSSAINNLLMNSLEAGAHHILLHGSIQDGRLVLAVVDDGKGLESGSEQRVMEPFFTTKAQGTGLGLAVVQTVV
RNHGGELKMQCAANRGCSVMLKFPVISEDSVKEVSHA

Sequences:

>Translated_357_residues
MATKALAKIADFPVRARLQEVKSSGVASAEQMAQILEAMPSGVVILNADGIVTLANPVAVQLLDRPLEGERWLSVIARAF
SPQDDDGHEVSLKNGRRVKLAITPLEPGQLILLTDLTETRLLQRNLAHLQRLSALGKMVATLAHQIRTPLSAALLYAANL
ASPKLNQDSRSRFQQKLVDRLNELERQVNDMLLMARGKVEAPMSSMPLADIIAPVLATCEPIATDKGCAVRFQDETLGAM
MAASQSALSSAINNLLMNSLEAGAHHILLHGSIQDGRLVLAVVDDGKGLESGSEQRVMEPFFTTKAQGTGLGLAVVQTVV
RNHGGELKMQCAANRGCSVMLKFPVISEDSVKEVSHA
>Mature_356_residues
ATKALAKIADFPVRARLQEVKSSGVASAEQMAQILEAMPSGVVILNADGIVTLANPVAVQLLDRPLEGERWLSVIARAFS
PQDDDGHEVSLKNGRRVKLAITPLEPGQLILLTDLTETRLLQRNLAHLQRLSALGKMVATLAHQIRTPLSAALLYAANLA
SPKLNQDSRSRFQQKLVDRLNELERQVNDMLLMARGKVEAPMSSMPLADIIAPVLATCEPIATDKGCAVRFQDETLGAMM
AASQSALSSAINNLLMNSLEAGAHHILLHGSIQDGRLVLAVVDDGKGLESGSEQRVMEPFFTTKAQGTGLGLAVVQTVVR
NHGGELKMQCAANRGCSVMLKFPVISEDSVKEVSHA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=28.8793103448276, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788549, Length=350, Percent_Identity=24.8571428571429, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=24.6808510638298, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=214, Percent_Identity=28.5046728971963, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786600, Length=344, Percent_Identity=22.6744186046512, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786783, Length=266, Percent_Identity=27.4436090225564, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38326; Mature: 38195

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKALAKIADFPVRARLQEVKSSGVASAEQMAQILEAMPSGVVILNADGIVTLANPVAV
CCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEECCHHHH
QLLDRPLEGERWLSVIARAFSPQDDDGHEVSLKNGRRVKLAITPLEPGQLILLTDLTETR
HHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECCCCCCEEEEECCHHHH
LLQRNLAHLQRLSALGKMVATLAHQIRTPLSAALLYAANLASPKLNQDSRSRFQQKLVDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LNELERQVNDMLLMARGKVEAPMSSMPLADIIAPVLATCEPIATDKGCAVRFQDETLGAM
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHH
MAASQSALSSAINNLLMNSLEAGAHHILLHGSIQDGRLVLAVVDDGKGLESGSEQRVMEP
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHH
FFTTKAQGTGLGLAVVQTVVRNHGGELKMQCAANRGCSVMLKFPVISEDSVKEVSHA
HHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHCCC
>Mature Secondary Structure 
ATKALAKIADFPVRARLQEVKSSGVASAEQMAQILEAMPSGVVILNADGIVTLANPVAV
CHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEECCHHHH
QLLDRPLEGERWLSVIARAFSPQDDDGHEVSLKNGRRVKLAITPLEPGQLILLTDLTETR
HHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECCCCCCEEEEECCHHHH
LLQRNLAHLQRLSALGKMVATLAHQIRTPLSAALLYAANLASPKLNQDSRSRFQQKLVDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LNELERQVNDMLLMARGKVEAPMSSMPLADIIAPVLATCEPIATDKGCAVRFQDETLGAM
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHH
MAASQSALSSAINNLLMNSLEAGAHHILLHGSIQDGRLVLAVVDDGKGLESGSEQRVMEP
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHH
FFTTKAQGTGLGLAVVQTVVRNHGGELKMQCAANRGCSVMLKFPVISEDSVKEVSHA
HHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]