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The map label for this gene is rfbD [H]

Identifier: 119775374

GI number: 119775374

Start: 2702369

End: 2703274

Strand: Reverse

Name: rfbD [H]

Synonym: Sama_2239

Alternate gene names: 119775374

Gene position: 2703274-2702369 (Counterclockwise)

Preceding gene: 119775375

Following gene: 119775373

Centisome position: 62.78

GC content: 52.76

Gene sequence:

>906_bases
ATGAGAGTGTTGTTGCTGGGCTCCAGCGGCCAGGTCGGCAGAGCGTTGCTGGCCTTCAAGCCTGCAGGTTACAAGTTACT
GGTCCCAGGCAGAAGCGAAGTGGATTATTTGCAACCTGAATCCATCACTGATTATGTGTTATTTCATAAGCCGCAATTGG
TCATCAACTGCGCGGCGTATACTGCTGTGGATAAGGCTGAAGCAGAGACAGCACTGTGTACGCGAATCAATAGCGGGGCT
TGCGAGCATCTCGCCAAGGCAGCAGCAGCGGCTGACGCCGTCCTGCTACATCTCTCTACCGATTATGTGTTTGATGGTCT
CCTCGACCGTCCATACAACGAAGAAGACTCGCCAGCCCCTTTGTCTGTGTATGGACATTCCAAGTGGTTGGGAGAGCAAA
CCATAGTCGCCAACTGTGCCAAGCATCTTATCGTCAGAACATCCTGGATTTTTGATGCTGACAGTAACAACTTCGTCAAT
ACCATGCTGCGTTTGGCGGCATCCAGGTCAGAGCTACGGGTTGTGGCAGACCAAGTTGGGGGACCGACGCCCGCATCTGA
ACTGGCCCGGGCGATTTGGCAACTTGCCAGGCAATCGGTGGAGAGAAATGGCCCTTGGGGCGTCTATCACTTTTCCGGCC
AACCCTTTGTCAGTTGGTACGAGTTTGCCAGGAGCATTTTGACTGAGGCATACAGGCTTTGCGTCATCCACCAACTACCA
ACAATAACATCTATCAGCGCCAGTGAGTTCGGCTCCCCTGCGCAGAGGCCTGCAAACTCGAGGCTTGATGGCAGTAAAAT
CAAAGCATTATATGGGGTTGCTGCCGCAGACTGGCAGAGTGAGTTAGTTAACATTCTGAAAGCCAAGGCCAGGGCCGTGA
CCTCAGTTACAAACCCTGGTTGTTAG

Upstream 100 bases:

>100_bases
GGAACTTGGAATCTAGAATCTAGAAGCGAAGCGAAGCGCTCTGTTTTTTCTGGCAGAGCTTTAGCCCGCTCTGTTTTCTT
ACAATCAAGGTGATGGTGTG

Downstream 100 bases:

>100_bases
AGGCTGTTGACACTTCGAGATTAAATTTTGTTCGCTCTGGCGAGCCTGTGCTCACGAAACGAGGAATGATGTGTAGTTAT
TCTACTCAAGTGACGAGTGA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRVLLLGSSGQVGRALLAFKPAGYKLLVPGRSEVDYLQPESITDYVLFHKPQLVINCAAYTAVDKAEAETALCTRINSGA
CEHLAKAAAAADAVLLHLSTDYVFDGLLDRPYNEEDSPAPLSVYGHSKWLGEQTIVANCAKHLIVRTSWIFDADSNNFVN
TMLRLAASRSELRVVADQVGGPTPASELARAIWQLARQSVERNGPWGVYHFSGQPFVSWYEFARSILTEAYRLCVIHQLP
TITSISASEFGSPAQRPANSRLDGSKIKALYGVAAADWQSELVNILKAKARAVTSVTNPGC

Sequences:

>Translated_301_residues
MRVLLLGSSGQVGRALLAFKPAGYKLLVPGRSEVDYLQPESITDYVLFHKPQLVINCAAYTAVDKAEAETALCTRINSGA
CEHLAKAAAAADAVLLHLSTDYVFDGLLDRPYNEEDSPAPLSVYGHSKWLGEQTIVANCAKHLIVRTSWIFDADSNNFVN
TMLRLAASRSELRVVADQVGGPTPASELARAIWQLARQSVERNGPWGVYHFSGQPFVSWYEFARSILTEAYRLCVIHQLP
TITSISASEFGSPAQRPANSRLDGSKIKALYGVAAADWQSELVNILKAKARAVTSVTNPGC
>Mature_301_residues
MRVLLLGSSGQVGRALLAFKPAGYKLLVPGRSEVDYLQPESITDYVLFHKPQLVINCAAYTAVDKAEAETALCTRINSGA
CEHLAKAAAAADAVLLHLSTDYVFDGLLDRPYNEEDSPAPLSVYGHSKWLGEQTIVANCAKHLIVRTSWIFDADSNNFVN
TMLRLAASRSELRVVADQVGGPTPASELARAIWQLARQSVERNGPWGVYHFSGQPFVSWYEFARSILTEAYRLCVIHQLP
TITSISASEFGSPAQRPANSRLDGSKIKALYGVAAADWQSELVNILKAKARAVTSVTNPGC

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=281, Percent_Identity=30.2491103202847, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI33519455, Length=281, Percent_Identity=30.2491103202847, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788352, Length=291, Percent_Identity=38.4879725085911, Blast_Score=204, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32736; Mature: 32736

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLLGSSGQVGRALLAFKPAGYKLLVPGRSEVDYLQPESITDYVLFHKPQLVINCAAY
CEEEEECCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEECCEEEEEEHHH
TAVDKAEAETALCTRINSGACEHLAKAAAAADAVLLHLSTDYVFDGLLDRPYNEEDSPAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECHHHHHHHHHCCCCCCCCCCCC
LSVYGHSKWLGEQTIVANCAKHLIVRTSWIFDADSNNFVNTMLRLAASRSELRVVADQVG
EEEECCCHHCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHCC
GPTPASELARAIWQLARQSVERNGPWGVYHFSGQPFVSWYEFARSILTEAYRLCVIHQLP
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
TITSISASEFGSPAQRPANSRLDGSKIKALYGVAAADWQSELVNILKAKARAVTSVTNPG
CCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
C
C
>Mature Secondary Structure
MRVLLLGSSGQVGRALLAFKPAGYKLLVPGRSEVDYLQPESITDYVLFHKPQLVINCAAY
CEEEEECCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEECCEEEEEEHHH
TAVDKAEAETALCTRINSGACEHLAKAAAAADAVLLHLSTDYVFDGLLDRPYNEEDSPAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECHHHHHHHHHCCCCCCCCCCCC
LSVYGHSKWLGEQTIVANCAKHLIVRTSWIFDADSNNFVNTMLRLAASRSELRVVADQVG
EEEECCCHHCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHCC
GPTPASELARAIWQLARQSVERNGPWGVYHFSGQPFVSWYEFARSILTEAYRLCVIHQLP
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
TITSISASEFGSPAQRPANSRLDGSKIKALYGVAAADWQSELVNILKAKARAVTSVTNPG
CCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
C
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]