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The map label for this gene is gltX

Identifier: 119775342

GI number: 119775342

Start: 2667907

End: 2669316

Strand: Reverse

Name: gltX

Synonym: Sama_2207

Alternate gene names: 119775342

Gene position: 2669316-2667907 (Counterclockwise)

Preceding gene: 119775349

Following gene: 119775340

Centisome position: 61.99

GC content: 52.62

Gene sequence:

>1410_bases
ATGACTGTAAAAACCCGTTTTGCTCCCAGCCCCACTGGATTCCTGCACGTTGGTGGTGCCCGTACAGCGCTGTATTCATG
GCTTCACGCCCGCGCCAATCAGGGGGAGTTTGTGTTGCGTATCGAAGATACCGACCTTGAGCGCTCAACCCAAGAAGCAG
TAGACGCTATTTTGGAAGGAATGGAATGGCTGAACCTGAACTGGGATGAGGGTCCTTACTATCAGACCAAGCGATTTGAC
CGGTACAACGAAATTATCGATCAAATGCTCAAGGCCGGTACGGCATACAAATGTTATTGCTCCAAAGAGCGTCTGGAAGC
CGTGCGTGAAGAGCAGGCCGCCAAGGGTGAGCGTCAGAAGTATGATGGTTGCTGCCGCGGCGCCGCCCCTCGCGCTGAAG
GTGAACCTCATGTAGTGCGCTTCCTGAATCCTCAGGGAGGCAGTGTGGTGTTTGATGACCACGTGCGTGGCCGTATCGAA
ATTGCCAACGAAGAGCTGGACGACCTTATCATTCGCCGTACCGATGGCAGCCCAACCTACAACTTCTGCGTGGTAGTGGA
CGATTGGGACATGGGCATAACCCACGTGGTGCGCGGTGAAGACCATATCAACAACACCCCACGTCAGATCAATATTCTCA
AAGCACTCGGTGCACCTATTCCTGAATACGCCCACGTTTCCATGATCCTGGGTGATGATGGTGCCAAGCTGTCCAAGCGC
CACGGCGCTGTGAGTGTGATGCAGTACCGTGACGACGGTTATCTGCCAGAAGCCTTGTTGAACTATCTGGTGCGTCTGGG
CTGGTCCCATGGCGATCAGGAAATCTTCTCCATGGATGAATTGGTGGAATTTTTCCGTCTCGATGACATCAACAAGGCCG
CCTCTGCCTTTAACAGCGAAAAACTGCTGTGGCTGAACCAGCACTATATTAAAGCTTTGGATCCTGAGTATGTGGCCAAG
CACCTTGAGTGGCACATGAAGGATCAGGGTATCGATACCAGCAATGGTCCGGCCCTTGCCGACGTGGTGACTGCGTTGTC
TGAGCGCGCCAAAACATTGAAAGAACTGGCTGCATCAAGCCGCTACTTCTATGAAGACTTTGAAGAATTCGATGCCGACC
AGGCCAAAAAGCATCTGCGTGGTGTGGCACTCGAGCCTTTGAAACTGGTTCAGCAGAAACTGGCTGCCCTGACTGAGTGG
ACTCGCGAAGCAATTCATCAAGCCATTGAAGAGACAGCAACTGAACTGGAAGTCGGTATGGGTAAAGTGGGCATGCCACT
GCGCGTTGCCGTGACCGGGGCAGGGCAGTCACCTGGATTGGATATCACCTTGGAACTCATAGGAAAAGCCCGCTCTGAGC
AAAGAATCTCCAAAGCGGTTGATTTTGTAGCAGATAGGATAAATTCCTAA

Upstream 100 bases:

>100_bases
GGACATGAGCCCATTTTCCAACGGCCCAATACACTGGTAGAATACTGCCACTTTGACCCTTAAGGCCCGATGGCCCTTTG
CTAATAGGTTAGTGTCCCCT

Downstream 100 bases:

>100_bases
AAAAGGCGTTGACACATTTTGGTGTGCTGCATAGAATGCGCCACGCAGTCGAGACACGGTGTCAGCAGCGCTGGCAGCAC
ACTCAAATGCAGTAAGAGAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTVKTRFAPSPTGFLHVGGARTALYSWLHARANQGEFVLRIEDTDLERSTQEAVDAILEGMEWLNLNWDEGPYYQTKRFD
RYNEIIDQMLKAGTAYKCYCSKERLEAVREEQAAKGERQKYDGCCRGAAPRAEGEPHVVRFLNPQGGSVVFDDHVRGRIE
IANEELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSMDELVEFFRLDDINKAASAFNSEKLLWLNQHYIKALDPEYVAK
HLEWHMKDQGIDTSNGPALADVVTALSERAKTLKELAASSRYFYEDFEEFDADQAKKHLRGVALEPLKLVQQKLAALTEW
TREAIHQAIEETATELEVGMGKVGMPLRVAVTGAGQSPGLDITLELIGKARSEQRISKAVDFVADRINS

Sequences:

>Translated_469_residues
MTVKTRFAPSPTGFLHVGGARTALYSWLHARANQGEFVLRIEDTDLERSTQEAVDAILEGMEWLNLNWDEGPYYQTKRFD
RYNEIIDQMLKAGTAYKCYCSKERLEAVREEQAAKGERQKYDGCCRGAAPRAEGEPHVVRFLNPQGGSVVFDDHVRGRIE
IANEELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSMDELVEFFRLDDINKAASAFNSEKLLWLNQHYIKALDPEYVAK
HLEWHMKDQGIDTSNGPALADVVTALSERAKTLKELAASSRYFYEDFEEFDADQAKKHLRGVALEPLKLVQQKLAALTEW
TREAIHQAIEETATELEVGMGKVGMPLRVAVTGAGQSPGLDITLELIGKARSEQRISKAVDFVADRINS
>Mature_468_residues
TVKTRFAPSPTGFLHVGGARTALYSWLHARANQGEFVLRIEDTDLERSTQEAVDAILEGMEWLNLNWDEGPYYQTKRFDR
YNEIIDQMLKAGTAYKCYCSKERLEAVREEQAAKGERQKYDGCCRGAAPRAEGEPHVVRFLNPQGGSVVFDDHVRGRIEI
ANEELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKRH
GAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSMDELVEFFRLDDINKAASAFNSEKLLWLNQHYIKALDPEYVAKH
LEWHMKDQGIDTSNGPALADVVTALSERAKTLKELAASSRYFYEDFEEFDADQAKKHLRGVALEPLKLVQQKLAALTEWT
REAIHQAIEETATELEVGMGKVGMPLRVAVTGAGQSPGLDITLELIGKARSEQRISKAVDFVADRINS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=332, Percent_Identity=37.3493975903614, Blast_Score=219, Evalue=5e-57,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=75.4797441364606, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=243, Percent_Identity=38.2716049382716, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1786895, Length=120, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=475, Percent_Identity=30.9473684210526, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=31.4285714285714, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6321192, Length=234, Percent_Identity=30.3418803418803, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=120, Percent_Identity=30, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24665321, Length=500, Percent_Identity=30.4, Blast_Score=242, Evalue=5e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_SHEAM (A1S7Q6)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928082.1
- ProteinModelPortal:   A1S7Q6
- SMR:   A1S7Q6
- STRING:   A1S7Q6
- GeneID:   4605999
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2207
- NMPDR:   fig|326297.7.peg.2072
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   A1S7Q6
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52814; Mature: 52683

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTRFAPSPTGFLHVGGARTALYSWLHARANQGEFVLRIEDTDLERSTQEAVDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
MEWLNLNWDEGPYYQTKRFDRYNEIIDQMLKAGTAYKCYCSKERLEAVREEQAAKGERQK
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCHHH
YDGCCRGAAPRAEGEPHVVRFLNPQGGSVVFDDHVRGRIEIANEELDDLIIRRTDGSPTY
HCCHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEEECCCCHHHHEEEECCCCCCE
NFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSMDELVEFFRLDDINKAASAFNSE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCC
KLLWLNQHYIKALDPEYVAKHLEWHMKDQGIDTSNGPALADVVTALSERAKTLKELAASS
CEEEECHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RYFYEDFEEFDADQAKKHLRGVALEPLKLVQQKLAALTEWTREAIHQAIEETATELEVGM
CHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKVGMPLRVAVTGAGQSPGLDITLELIGKARSEQRISKAVDFVADRINS
CCCCCCEEEEEECCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVKTRFAPSPTGFLHVGGARTALYSWLHARANQGEFVLRIEDTDLERSTQEAVDAILEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
MEWLNLNWDEGPYYQTKRFDRYNEIIDQMLKAGTAYKCYCSKERLEAVREEQAAKGERQK
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCHHH
YDGCCRGAAPRAEGEPHVVRFLNPQGGSVVFDDHVRGRIEIANEELDDLIIRRTDGSPTY
HCCHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEEECCCCHHHHEEEECCCCCCE
NFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSMDELVEFFRLDDINKAASAFNSE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCC
KLLWLNQHYIKALDPEYVAKHLEWHMKDQGIDTSNGPALADVVTALSERAKTLKELAASS
CEEEECHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RYFYEDFEEFDADQAKKHLRGVALEPLKLVQQKLAALTEWTREAIHQAIEETATELEVGM
CHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKVGMPLRVAVTGAGQSPGLDITLELIGKARSEQRISKAVDFVADRINS
CCCCCCEEEEEECCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA