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The map label for this gene is topB [H]

Identifier: 119775280

GI number: 119775280

Start: 2589979

End: 2592063

Strand: Reverse

Name: topB [H]

Synonym: Sama_2145

Alternate gene names: 119775280

Gene position: 2592063-2589979 (Counterclockwise)

Preceding gene: 119775281

Following gene: 119775277

Centisome position: 60.19

GC content: 55.73

Gene sequence:

>2085_bases
TTGCGCCCACCGGGAATTTTCGCTACATTTACTGTAAATTCATCCAGTGCTTTTGCACCAGAATATCCCGCCATGATCCT
CTATATCGCCGAAAAACCGAGCCTCGGCCGCGCCATCGCTGAGGTGTTACCCAAACCCCATAAAAAGGGTGAGGGTTATA
TTGAGCTTGGCAACGGCGATTGTGTCACCTGGTGTATTGGCCACTTACTGGAGCAGGCCGAGCCGGATGCGTATTCTCCC
GAATACAAGCAGTGGCAGCTTGCCCATCTGCCCATTATTCCCGCCGAGTGGAAACTCACTCCACGAAGTTCGGCCAAAAG
TCAGCTGGCAGTCGTAAAGCGCCTGATTAAACAGGCTTCTTCTTTGGTTCATGCAGGGGACCCGGACCGCGAAGGACAGC
TTCTGGTGGATGAGGTAATTTCCCACGCCAAGGTTTCTGCATCCAAATTGGCCTCCACCCAGCGCTTATTGATTTCCGAT
TTAAACCCCGACGCGGTGCGCCGCGCACTGGGACAGCTTCGCAGCAATAAGGAGTTTGTGCCGCTTGCCACATCGGCATT
GGCGCGCAGTCGTGCCGACTGGCTTTACGGCCTGAATATGACCCGTGCTTACACGCTTCAGGGCAGAAAGGTGGGGTATG
CAGGGGTGTTGTCTGTCGGACGGGTGCAAACCCCATTATTGGGTTTGGTGGTTCGCCGTGACCTGGACATTGAGCACTTT
GAGCCCAAAGATCACTATGAGGTGCTTGCTCACATTACCACAGACAAGGGCGAGTCCTTTTGTGCCAAGTGGCAACCCAG
CGAAGCTTGTCAGCCGTACATGGATGAAGAGGGGCGGGTGCTTTCACGCCGCCTCGCCGAAACTGTGGTGAGCCGCATTG
GCGGTAAGCCGGCACTGGTTGAGCATGTCAGCCATAAAGACAAGAAGCAGGCGCCGCCATTGCTGTATAGCCTCTCGGCG
CTGCAAATCGATTGCGCCAAACGTTTTGGGATGAGTGCCAAAGAGGTGCTGGATACGGCCCAGAGCCTGTATGAGCGACA
CAAGCTGATTACCTATCCCCGCTCTGACAGCCGTCATTTACCCAAAGAACAACTTGCTTTGGTGCCTGATGTGCTGTCGG
CCGTCAGTGCCAACGCCCCTGAGCTTTTGGAAGGGGCGCCGTCACCTGATTTGCACTTGGTGTCCAAGGCCTGGGATGAC
AGTAAGGTGGATGCCCACCACGCCATAGTGCCAACGGGCAAACGAACGGCGCTGTCGCAACTTAACCAGCGGGAGCGTCA
GCTTTATTTGCACATTGCACGGCAATATTTGGCGCAGTTTTACCCCAATCGCCTTCTGGCAGATACCGAAATCCAGCTGA
TTATCGAAGGCGGTAGCTTTATCGCAAGGGCGTCGGTTGAGCGAGCCCCAGGTTGGCGGGTATTGTTTCAGGGACGCAGC
ACAGAGCAGGACGAGGCGAAAGAGCCACAAGAGACTGATGCCAGCCAAAAGACGCCCTTGCCAGCCCTGAATAAGGGCGA
CATTGCCCGGTGCTGTCATGGTGAGTTATTGGCAAAGCAAACCCAACCCCCAAAACACTTTACCGATGCGACCTTGCTCA
GTGCCATGACCGGCATTGCCCGTTATGTGAGTTCACCGGACATTAAACGTATATTGAAGGACACGGATGGCCTTGGCACC
GAAGCGACCCGGGCCGGGATCATCGAACTTCTGTTTAAACGTGGCTTTCTGGTGCGCCAGGGTAAAAGCATCGTGGCATC
CCAAGCCGGGCGGGGGTTAATTGCCAGCTTGCCTGAGGTGGCCACCACTGCCGATATGACCGCCCTGTGGGAGCGTCAGC
TCAGCGATATTTGCCAACGTCAGCTTTCCTATAAAGGCTTTATGACACCACTGGTATTGGAACTTGAAAAGTTGATTGAG
CAGGCCGCTGCCAGTTTACCGACCGCTCTGGCCGGCGTGACAGCGGCAGGGCCTAAACGGTCTTTCAGAGGCAGAGCTGG
CAAGGGGAAAGGGCGTGCTCCCGGGGCAAGTGCTGGCAGAGGTCGCAAAAGCCGGTCAAGCCCAAGCGGCAAGGTTGCAA
CTTGA

Upstream 100 bases:

>100_bases
ATATCGGTCATTTCAGCTTTGATGAGAACTATTCTCAACAAAATTTACATAGGATCAGGGTAAATTTGACCTTGGCTTTG
CACTTGGCCAGGATTGCGCG

Downstream 100 bases:

>100_bases
GCAGATATGGCAACGTGAGCCAGCTAGCCGAGATGAACTGAAGCACCCATCAGGCGATACCCAGGGTATCCCAGGATTCA
GAATAGCTTCACTTTGGTAT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 694; Mature: 694

Protein sequence:

>694_residues
MRPPGIFATFTVNSSSAFAPEYPAMILYIAEKPSLGRAIAEVLPKPHKKGEGYIELGNGDCVTWCIGHLLEQAEPDAYSP
EYKQWQLAHLPIIPAEWKLTPRSSAKSQLAVVKRLIKQASSLVHAGDPDREGQLLVDEVISHAKVSASKLASTQRLLISD
LNPDAVRRALGQLRSNKEFVPLATSALARSRADWLYGLNMTRAYTLQGRKVGYAGVLSVGRVQTPLLGLVVRRDLDIEHF
EPKDHYEVLAHITTDKGESFCAKWQPSEACQPYMDEEGRVLSRRLAETVVSRIGGKPALVEHVSHKDKKQAPPLLYSLSA
LQIDCAKRFGMSAKEVLDTAQSLYERHKLITYPRSDSRHLPKEQLALVPDVLSAVSANAPELLEGAPSPDLHLVSKAWDD
SKVDAHHAIVPTGKRTALSQLNQRERQLYLHIARQYLAQFYPNRLLADTEIQLIIEGGSFIARASVERAPGWRVLFQGRS
TEQDEAKEPQETDASQKTPLPALNKGDIARCCHGELLAKQTQPPKHFTDATLLSAMTGIARYVSSPDIKRILKDTDGLGT
EATRAGIIELLFKRGFLVRQGKSIVASQAGRGLIASLPEVATTADMTALWERQLSDICQRQLSYKGFMTPLVLELEKLIE
QAAASLPTALAGVTAAGPKRSFRGRAGKGKGRAPGASAGRGRKSRSSPSGKVAT

Sequences:

>Translated_694_residues
MRPPGIFATFTVNSSSAFAPEYPAMILYIAEKPSLGRAIAEVLPKPHKKGEGYIELGNGDCVTWCIGHLLEQAEPDAYSP
EYKQWQLAHLPIIPAEWKLTPRSSAKSQLAVVKRLIKQASSLVHAGDPDREGQLLVDEVISHAKVSASKLASTQRLLISD
LNPDAVRRALGQLRSNKEFVPLATSALARSRADWLYGLNMTRAYTLQGRKVGYAGVLSVGRVQTPLLGLVVRRDLDIEHF
EPKDHYEVLAHITTDKGESFCAKWQPSEACQPYMDEEGRVLSRRLAETVVSRIGGKPALVEHVSHKDKKQAPPLLYSLSA
LQIDCAKRFGMSAKEVLDTAQSLYERHKLITYPRSDSRHLPKEQLALVPDVLSAVSANAPELLEGAPSPDLHLVSKAWDD
SKVDAHHAIVPTGKRTALSQLNQRERQLYLHIARQYLAQFYPNRLLADTEIQLIIEGGSFIARASVERAPGWRVLFQGRS
TEQDEAKEPQETDASQKTPLPALNKGDIARCCHGELLAKQTQPPKHFTDATLLSAMTGIARYVSSPDIKRILKDTDGLGT
EATRAGIIELLFKRGFLVRQGKSIVASQAGRGLIASLPEVATTADMTALWERQLSDICQRQLSYKGFMTPLVLELEKLIE
QAAASLPTALAGVTAAGPKRSFRGRAGKGKGRAPGASAGRGRKSRSSPSGKVAT
>Mature_694_residues
MRPPGIFATFTVNSSSAFAPEYPAMILYIAEKPSLGRAIAEVLPKPHKKGEGYIELGNGDCVTWCIGHLLEQAEPDAYSP
EYKQWQLAHLPIIPAEWKLTPRSSAKSQLAVVKRLIKQASSLVHAGDPDREGQLLVDEVISHAKVSASKLASTQRLLISD
LNPDAVRRALGQLRSNKEFVPLATSALARSRADWLYGLNMTRAYTLQGRKVGYAGVLSVGRVQTPLLGLVVRRDLDIEHF
EPKDHYEVLAHITTDKGESFCAKWQPSEACQPYMDEEGRVLSRRLAETVVSRIGGKPALVEHVSHKDKKQAPPLLYSLSA
LQIDCAKRFGMSAKEVLDTAQSLYERHKLITYPRSDSRHLPKEQLALVPDVLSAVSANAPELLEGAPSPDLHLVSKAWDD
SKVDAHHAIVPTGKRTALSQLNQRERQLYLHIARQYLAQFYPNRLLADTEIQLIIEGGSFIARASVERAPGWRVLFQGRS
TEQDEAKEPQETDASQKTPLPALNKGDIARCCHGELLAKQTQPPKHFTDATLLSAMTGIARYVSSPDIKRILKDTDGLGT
EATRAGIIELLFKRGFLVRQGKSIVASQAGRGLIASLPEVATTADMTALWERQLSDICQRQLSYKGFMTPLVLELEKLIE
QAAASLPTALAGVTAAGPKRSFRGRAGKGKGRAPGASAGRGRKSRSSPSGKVAT

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=632, Percent_Identity=23.5759493670886, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1788061, Length=666, Percent_Identity=56.7567567567568, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=576, Percent_Identity=24.3055555555556, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563869, Length=654, Percent_Identity=23.394495412844, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323263, Length=703, Percent_Identity=22.7596017069701, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585251, Length=616, Percent_Identity=23.3766233766234, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 75947; Mature: 75947

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPGIFATFTVNSSSAFAPEYPAMILYIAEKPSLGRAIAEVLPKPHKKGEGYIELGNGD
CCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCC
CVTWCIGHLLEQAEPDAYSPEYKQWQLAHLPIIPAEWKLTPRSSAKSQLAVVKRLIKQAS
CHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHH
SLVHAGDPDREGQLLVDEVISHAKVSASKLASTQRLLISDLNPDAVRRALGQLRSNKEFV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
PLATSALARSRADWLYGLNMTRAYTLQGRKVGYAGVLSVGRVQTPLLGLVVRRDLDIEHF
CHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCCC
EPKDHYEVLAHITTDKGESFCAKWQPSEACQPYMDEEGRVLSRRLAETVVSRIGGKPALV
CCCHHHHHHHEEECCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
EHVSHKDKKQAPPLLYSLSALQIDCAKRFGMSAKEVLDTAQSLYERHKLITYPRSDSRHL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCC
PKEQLALVPDVLSAVSANAPELLEGAPSPDLHLVSKAWDDSKVDAHHAIVPTGKRTALSQ
CHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEHHHCCCCCCCCCCCEECCCCHHHHHHH
LNQRERQLYLHIARQYLAQFYPNRLLADTEIQLIIEGGSFIARASVERAPGWRVLFQGRS
HHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEECCCEEEECCCCCCCCCEEEEECCC
TEQDEAKEPQETDASQKTPLPALNKGDIARCCHGELLAKQTQPPKHFTDATLLSAMTGIA
CCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RYVSSPDIKRILKDTDGLGTEATRAGIIELLFKRGFLVRQGKSIVASQAGRGLIASLPEV
HHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHCCCCHHHHCCHH
ATTADMTALWERQLSDICQRQLSYKGFMTPLVLELEKLIEQAAASLPTALAGVTAAGPKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCH
SFRGRAGKGKGRAPGASAGRGRKSRSSPSGKVAT
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPPGIFATFTVNSSSAFAPEYPAMILYIAEKPSLGRAIAEVLPKPHKKGEGYIELGNGD
CCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCC
CVTWCIGHLLEQAEPDAYSPEYKQWQLAHLPIIPAEWKLTPRSSAKSQLAVVKRLIKQAS
CHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHH
SLVHAGDPDREGQLLVDEVISHAKVSASKLASTQRLLISDLNPDAVRRALGQLRSNKEFV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
PLATSALARSRADWLYGLNMTRAYTLQGRKVGYAGVLSVGRVQTPLLGLVVRRDLDIEHF
CHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCCC
EPKDHYEVLAHITTDKGESFCAKWQPSEACQPYMDEEGRVLSRRLAETVVSRIGGKPALV
CCCHHHHHHHEEECCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
EHVSHKDKKQAPPLLYSLSALQIDCAKRFGMSAKEVLDTAQSLYERHKLITYPRSDSRHL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCC
PKEQLALVPDVLSAVSANAPELLEGAPSPDLHLVSKAWDDSKVDAHHAIVPTGKRTALSQ
CHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEHHHCCCCCCCCCCCEECCCCHHHHHHH
LNQRERQLYLHIARQYLAQFYPNRLLADTEIQLIIEGGSFIARASVERAPGWRVLFQGRS
HHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEECCCEEEECCCCCCCCCEEEEECCC
TEQDEAKEPQETDASQKTPLPALNKGDIARCCHGELLAKQTQPPKHFTDATLLSAMTGIA
CCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RYVSSPDIKRILKDTDGLGTEATRAGIIELLFKRGFLVRQGKSIVASQAGRGLIASLPEV
HHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHCCCCHHHHCCHH
ATTADMTALWERQLSDICQRQLSYKGFMTPLVLELEKLIEQAAASLPTALAGVTAAGPKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCH
SFRGRAGKGKGRAPGASAGRGRKSRSSPSGKVAT
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]