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The map label for this gene is purF [H]

Identifier: 119775281

GI number: 119775281

Start: 2592502

End: 2594016

Strand: Reverse

Name: purF [H]

Synonym: Sama_2146

Alternate gene names: 119775281

Gene position: 2594016-2592502 (Counterclockwise)

Preceding gene: 119775282

Following gene: 119775280

Centisome position: 60.24

GC content: 53.8

Gene sequence:

>1515_bases
ATGTGTGGTATCGTCGGAATAGTTGGCCATTCCTCGGTTAATCAGACCATCTATGACGCACTGACAGTGCTTCAACACCG
TGGTCAGGATGCAGCCGGGATAGTCACGGTAGACAATGACGCGTTCAGGCTCCGTAAGGCGAACGGACTGGTGCGTGATG
TGTTTGAACCCAAACACATGCAGCGCCTGCAGGGTAACGCCGGTATCGGCCATGTGCGTTATCCCACCGCCGGGAGCTCC
AGCTCCTCAGAAGCCCAGCCTTTTTACGTTAACTCGCCTTTTGGTATTTCTCTGGCGCACAACGGCAACCTGACCAACAC
TCAAGACCTGTCGGCTCGTCTTAAGCAGCAGCGTCGACACGTGAATACCACGTCGGATTCTGAAGTGCTGCTGAACCTGC
TCGCCGATGAGCTGCAGCAGGTACAGAGCGCCCATCTGAGCGCCGATCAGGTGTTTGATGCCGTGGCAAAAGTGCACGCC
CAAACCCGTGGTGCTTACGCGGTAGTGGCCATGATTGTTGGCGAAGGTCTTGTGGCCTTCCGTGACCCCAATGGCATTCG
CCCGCTGGTGCTTGGCAAAAATGAAACCGCCACCGGCACAGAGTACATGGTGGCCTCAGAGAGTGTGGCCCTGGATGCCG
TGGGTTTTGAAGTCATTCGTGATGTTGCGCCGGGCGAAGCCGTATACGTCACCCTCGACGGTCAGCTGTTTACCCGTCAG
TGCGCCGAAGCGCCGCTCTATTCGCCCTGTATTTTCGAATTTGTTTACTTTGCCCGTCCAGACTCCACCATCGACAAGGT
GTCTGTGTACGCCAGCCGGGTAAATATGGGCGCCAAACTGGGCGAGAAAATCAAGAAAGAATGGTACGACAACGACATCG
ATGTGGTTATCCCCATTCCTGAAACCTCCTGTGATATCGCACTGGAAATTGCCCGTGTTATGGACTTGCCTTACCGTCAG
GGCTTTGTAAAGAACCGCTACATTGGCCGTACCTTCATCATGCCTGGTCAGCAGGAGCGTAAGAAGTCAGTGCGCCGTAA
ACTCAATGCCATCAACACCGAGTTTAAGGGCAAGAATGTGCTTTTGGTGGACGACTCCATCGTGCGTGGTACCACGTCAG
AGCAAATCATCGAGATGGCCCGCGAAGCCGGTGCCAAGAAGGTGTATTTCGCCTCTGCTGCCCCTGAAATTCGTTTCCCC
AACGTGTACGGCATCGATATGCCCACTTCCAATGAGCTGATTGCCCACGGACGTGATGCGGATGAAATTGCCAAACTGAT
TGGTGCCGATGGTATCATCTTCCAGGATTTGACTGATCTGGTTGAAGCTGTGCGTATGGAAAACCCCGAAATCAAGCGTT
TTGAAACATCGGTGTTTGACGGTGATTACATCACTGGTGATGTGACTCAGGCGTATCTTGACCACCTCACTCAGCTGAGA
AACGATGATGCCAAGGCAAATCGCAACAGCGATGTGGGCACGAATCTCGAGCTGCACAACGTCTGTCACCCGTAA

Upstream 100 bases:

>100_bases
TCCAGTGGTTCTTCCACTATCTGGAAAACACCTCAAGCTTCGTACCCAAAATATAAAAATTAGCTAAACAACAGAACATC
TTAAAAGAGGACACTCTCCC

Downstream 100 bases:

>100_bases
TGGGTTACACCCATAATGATGGGTAGCTAAAAGATCAAGGCAAATCAAAACCAGCACTCAGGTGCTGGTTTTTGTTTTTG
GGAATTTAACATTCACACTC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGHSSVNQTIYDALTVLQHRGQDAAGIVTVDNDAFRLRKANGLVRDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SSSEAQPFYVNSPFGISLAHNGNLTNTQDLSARLKQQRRHVNTTSDSEVLLNLLADELQQVQSAHLSADQVFDAVAKVHA
QTRGAYAVVAMIVGEGLVAFRDPNGIRPLVLGKNETATGTEYMVASESVALDAVGFEVIRDVAPGEAVYVTLDGQLFTRQ
CAEAPLYSPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDNDIDVVIPIPETSCDIALEIARVMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQDLTDLVEAVRMENPEIKRFETSVFDGDYITGDVTQAYLDHLTQLR
NDDAKANRNSDVGTNLELHNVCHP

Sequences:

>Translated_504_residues
MCGIVGIVGHSSVNQTIYDALTVLQHRGQDAAGIVTVDNDAFRLRKANGLVRDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SSSEAQPFYVNSPFGISLAHNGNLTNTQDLSARLKQQRRHVNTTSDSEVLLNLLADELQQVQSAHLSADQVFDAVAKVHA
QTRGAYAVVAMIVGEGLVAFRDPNGIRPLVLGKNETATGTEYMVASESVALDAVGFEVIRDVAPGEAVYVTLDGQLFTRQ
CAEAPLYSPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDNDIDVVIPIPETSCDIALEIARVMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQDLTDLVEAVRMENPEIKRFETSVFDGDYITGDVTQAYLDHLTQLR
NDDAKANRNSDVGTNLELHNVCHP
>Mature_504_residues
MCGIVGIVGHSSVNQTIYDALTVLQHRGQDAAGIVTVDNDAFRLRKANGLVRDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SSSEAQPFYVNSPFGISLAHNGNLTNTQDLSARLKQQRRHVNTTSDSEVLLNLLADELQQVQSAHLSADQVFDAVAKVHA
QTRGAYAVVAMIVGEGLVAFRDPNGIRPLVLGKNETATGTEYMVASESVALDAVGFEVIRDVAPGEAVYVTLDGQLFTRQ
CAEAPLYSPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDNDIDVVIPIPETSCDIALEIARVMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQDLTDLVEAVRMENPEIKRFETSVFDGDYITGDVTQAYLDHLTQLR
NDDAKANRNSDVGTNLELHNVCHP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=36.0759493670886, Blast_Score=270, Evalue=3e-72,
Organism=Escherichia coli, GI1788651, Length=503, Percent_Identity=73.3598409542744, Blast_Score=769, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=215, Percent_Identity=31.6279069767442, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=464, Percent_Identity=32.7586206896552, Blast_Score=224, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6323958, Length=510, Percent_Identity=50.3921568627451, Blast_Score=492, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24659598, Length=480, Percent_Identity=35.625, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24659604, Length=473, Percent_Identity=35.7293868921776, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI28573187, Length=474, Percent_Identity=33.5443037974684, Blast_Score=252, Evalue=5e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55465; Mature: 55465

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGHSSVNQTIYDALTVLQHRGQDAAGIVTVDNDAFRLRKANGLVRDVFEPKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHCCHHHHHCCHHHH
QRLQGNAGIGHVRYPTAGSSSSSEAQPFYVNSPFGISLAHNGNLTNTQDLSARLKQQRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQQVQSAHLSADQVFDAVAKVHAQTRGAYAVVAMIVGEGLVAF
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEE
RDPNGIRPLVLGKNETATGTEYMVASESVALDAVGFEVIRDVAPGEAVYVTLDGQLFTRQ
ECCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
CAEAPLYSPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDNDIDVVIPIP
HHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARVMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
CCCCHHHHHHHHHHCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
DEIAKLIGADGIIFQDLTDLVEAVRMENPEIKRFETSVFDGDYITGDVTQAYLDHLTQLR
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHEEHHCCCCCCCCCHHHHHHHHHHHHHC
NDDAKANRNSDVGTNLELHNVCHP
CCCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MCGIVGIVGHSSVNQTIYDALTVLQHRGQDAAGIVTVDNDAFRLRKANGLVRDVFEPKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHCCHHHHHCCHHHH
QRLQGNAGIGHVRYPTAGSSSSSEAQPFYVNSPFGISLAHNGNLTNTQDLSARLKQQRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQQVQSAHLSADQVFDAVAKVHAQTRGAYAVVAMIVGEGLVAF
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEE
RDPNGIRPLVLGKNETATGTEYMVASESVALDAVGFEVIRDVAPGEAVYVTLDGQLFTRQ
ECCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
CAEAPLYSPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDNDIDVVIPIP
HHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARVMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
CCCCHHHHHHHHHHCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
DEIAKLIGADGIIFQDLTDLVEAVRMENPEIKRFETSVFDGDYITGDVTQAYLDHLTQLR
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHEEHHCCCCCCCCCHHHHHHHHHHHHHC
NDDAKANRNSDVGTNLELHNVCHP
CCCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]