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The map label for this gene is thiE [H]

Identifier: 119774994

GI number: 119774994

Start: 2262173

End: 2263750

Strand: Reverse

Name: thiE [H]

Synonym: Sama_1859

Alternate gene names: 119774994

Gene position: 2263750-2262173 (Counterclockwise)

Preceding gene: 119774995

Following gene: 119774993

Centisome position: 52.57

GC content: 58.62

Gene sequence:

>1578_bases
ATGGCCAAAATCAACGACATGGCAGCTAAACCCATCGTCTGGACTATCGCAGGCTCAGACAGCGGCGGCGGCGCCGGTAT
TCAGGCGGATTTGCTGACGCTGCAAGACCTGGGCTGCTATGGTTGCAGCGTGATAACCGCCATAACGGCGCAAAACTCGG
TGGCGGTGACGGCGGTTGAGCCGGTGACACCAAGGATGCTGCGTGCCCAGCTTGAGACGTTAAAGGCGGATTTGTACCCC
AGCGCAGTCAAGATAGGCCTTATCGCCAATCAGCCGCAGCTGGATTTTTTGGCTGACTGGCTGGGTGAAAACTTACCCGG
CGTGCCAGTTATTCTTGACCCTGTTATGGTGGCCTCCTGCGGTGGTGACCTGCAGTCGCCAGACAATACCTGCCCCGATA
AAACCGGGGAACGGTCTGGTCTGGATTTCAGTCCCTTTGCCGGAAAAATTAGCCTTATCACCCCCAACAAGGCGGAACTT
TCCCGTCTTATCGGCCGCTTACTCAATGATGACGATGCTCTGGAGTGGGCTGCCAACGTACTGTGGCAGGCCTTTGGCGG
CAGCATTCTTGCCAAGGGAGGAGATTGCCCATGGCAGCAGTCTAAGGCCAGGGATCTGTTGCTGCTGGGTGATATTGCTA
CCATTTCGCCGCTGCACAGGCGTCAGGGATTTTGGCTCAGCAATGAGCGGGTTGCGACTGTGCACAATCACGGCAGCGGC
TGCACGCTTTCAAGTGCCATAGCCGCCTTTGTCGCCAAGGGGTTGGTGCTGCCCGATGCGGTGCTGCTGGCCAAAGCCTA
TGTATTTGAGGGGCTCAGGCAGGCAAGCGCTGTAGGTTCTGGCCCCGGGCCACTGGCCCGCACCGGCATGCCTGAGTCCC
TGGCCGCTATGGCATCGATTCACCGATTACATGAAAGCCGCAGTCACAGGTTGCCAACCAAGGGGTTTACCCGCCTGGGT
CATGATTTGGGCGTTTATCCCGTGGTGGCCGACATCGATATGCTTGAAACACTGCTTGAGGCAGGCGCCAACCCAATTCA
GCTGCGCATCAAGGGCGAGATTGGCCCGGGTAGCGCGGCTGAAGCGGACATCATCCGCGCCATTGCCCTTGGCAGGCAGC
ATCGGGCGCGCGTCTTTATTAACGACCATTGGCGCCTTGCCATCAGCCATGGTGCCTTTGGGGTGCATCTTGGGCAGGAG
GATATAGAGGCCGTGCCGCTGGATGCCATTGCTGAGGCTGGCATGGCCCTTGGGCTGTCGAGTCACGGGTTGTTTGAGGC
CTTGCTGGCCCTTGAGCTTAACCCCAGCTACCTCGCCCTTGGGCATATTTTTCCCACTACCACCAAGGAAATGCCTTCCA
AACCACAGGGGCTGGAGACGCTGGCACTTCTTGCGAAGATTGTCAGTACAGCTGTGCCAACGGTGGCGATTGGCGGTATA
GAGGCGGGTAATTTGCCTGAGGTCGCTGCTACAGGCGTGGACAGCATTGCCGTGGTACGTGCTGTGACCGAAGCAAGCGA
ACCGGCGACTGCCTTTCGCGCACTCGCTGACGCCTGGGAGATGCGGAATGCTCGCTGA

Upstream 100 bases:

>100_bases
TATGGACAAGATGTCGGCCACCTTTAAACAGACAGGCAGTGCGCTTTACAGCGAGACTCATGTGGAAGCGGCCCTGAGCA
CCGAAAAGGAACCGGTGTAG

Downstream 100 bases:

>100_bases
TAAAGATTTTATCCGCTACTCGCGGCAAATTTTGCTGCCAGAATGCGGCGAAGCCGGGCAGCTCAGATTAAGGAACTCCC
ATGTGGCCATAGTGGGCGTG

Product: phosphomethylpyrimidine kinase/thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MAKINDMAAKPIVWTIAGSDSGGGAGIQADLLTLQDLGCYGCSVITAITAQNSVAVTAVEPVTPRMLRAQLETLKADLYP
SAVKIGLIANQPQLDFLADWLGENLPGVPVILDPVMVASCGGDLQSPDNTCPDKTGERSGLDFSPFAGKISLITPNKAEL
SRLIGRLLNDDDALEWAANVLWQAFGGSILAKGGDCPWQQSKARDLLLLGDIATISPLHRRQGFWLSNERVATVHNHGSG
CTLSSAIAAFVAKGLVLPDAVLLAKAYVFEGLRQASAVGSGPGPLARTGMPESLAAMASIHRLHESRSHRLPTKGFTRLG
HDLGVYPVVADIDMLETLLEAGANPIQLRIKGEIGPGSAAEADIIRAIALGRQHRARVFINDHWRLAISHGAFGVHLGQE
DIEAVPLDAIAEAGMALGLSSHGLFEALLALELNPSYLALGHIFPTTTKEMPSKPQGLETLALLAKIVSTAVPTVAIGGI
EAGNLPEVAATGVDSIAVVRAVTEASEPATAFRALADAWEMRNAR

Sequences:

>Translated_525_residues
MAKINDMAAKPIVWTIAGSDSGGGAGIQADLLTLQDLGCYGCSVITAITAQNSVAVTAVEPVTPRMLRAQLETLKADLYP
SAVKIGLIANQPQLDFLADWLGENLPGVPVILDPVMVASCGGDLQSPDNTCPDKTGERSGLDFSPFAGKISLITPNKAEL
SRLIGRLLNDDDALEWAANVLWQAFGGSILAKGGDCPWQQSKARDLLLLGDIATISPLHRRQGFWLSNERVATVHNHGSG
CTLSSAIAAFVAKGLVLPDAVLLAKAYVFEGLRQASAVGSGPGPLARTGMPESLAAMASIHRLHESRSHRLPTKGFTRLG
HDLGVYPVVADIDMLETLLEAGANPIQLRIKGEIGPGSAAEADIIRAIALGRQHRARVFINDHWRLAISHGAFGVHLGQE
DIEAVPLDAIAEAGMALGLSSHGLFEALLALELNPSYLALGHIFPTTTKEMPSKPQGLETLALLAKIVSTAVPTVAIGGI
EAGNLPEVAATGVDSIAVVRAVTEASEPATAFRALADAWEMRNAR
>Mature_524_residues
AKINDMAAKPIVWTIAGSDSGGGAGIQADLLTLQDLGCYGCSVITAITAQNSVAVTAVEPVTPRMLRAQLETLKADLYPS
AVKIGLIANQPQLDFLADWLGENLPGVPVILDPVMVASCGGDLQSPDNTCPDKTGERSGLDFSPFAGKISLITPNKAELS
RLIGRLLNDDDALEWAANVLWQAFGGSILAKGGDCPWQQSKARDLLLLGDIATISPLHRRQGFWLSNERVATVHNHGSGC
TLSSAIAAFVAKGLVLPDAVLLAKAYVFEGLRQASAVGSGPGPLARTGMPESLAAMASIHRLHESRSHRLPTKGFTRLGH
DLGVYPVVADIDMLETLLEAGANPIQLRIKGEIGPGSAAEADIIRAIALGRQHRARVFINDHWRLAISHGAFGVHLGQED
IEAVPLDAIAEAGMALGLSSHGLFEALLALELNPSYLALGHIFPTTTKEMPSKPQGLETLALLAKIVSTAVPTVAIGGIE
AGNLPEVAATGVDSIAVVRAVTEASEPATAFRALADAWEMRNAR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=184, Percent_Identity=55.4347826086957, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788420, Length=290, Percent_Identity=34.8275862068965, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6325378, Length=287, Percent_Identity=30.3135888501742, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324517, Length=280, Percent_Identity=32.5, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324997, Length=282, Percent_Identity=31.9148936170213, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 55137; Mature: 55006

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKINDMAAKPIVWTIAGSDSGGGAGIQADLLTLQDLGCYGCSVITAITAQNSVAVTAVE
CCCCCCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHCCCHHHHHHHHEECCCCEEEEEEC
PVTPRMLRAQLETLKADLYPSAVKIGLIANQPQLDFLADWLGENLPGVPVILDPVMVASC
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCEEECCHHHHHC
GGDLQSPDNTCPDKTGERSGLDFSPFAGKISLITPNKAELSRLIGRLLNDDDALEWAANV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHH
LWQAFGGSILAKGGDCPWQQSKARDLLLLGDIATISPLHRRQGFWLSNERVATVHNHGSG
HHHHHCCCEEECCCCCCCCCCCCCCEEEECCHHHHCHHHHHCCCEECCCEEEEEECCCCC
CTLSSAIAAFVAKGLVLPDAVLLAKAYVFEGLRQASAVGSGPGPLARTGMPESLAAMASI
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
HRLHESRSHRLPTKGFTRLGHDLGVYPVVADIDMLETLLEAGANPIQLRIKGEIGPGSAA
HHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
EADIIRAIALGRQHRARVFINDHWRLAISHGAFGVHLGQEDIEAVPLDAIAEAGMALGLS
HHHHHHHHHHCCCCCEEEEEECCEEEEEECCCEEEECCHHHHHHCCHHHHHHCCHHCCCC
SHGLFEALLALELNPSYLALGHIFPTTTKEMPSKPQGLETLALLAKIVSTAVPTVAIGGI
CCHHHHHHHHEECCCCEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCEEEECCC
EAGNLPEVAATGVDSIAVVRAVTEASEPATAFRALADAWEMRNAR
CCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKINDMAAKPIVWTIAGSDSGGGAGIQADLLTLQDLGCYGCSVITAITAQNSVAVTAVE
CCCCCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHCCCHHHHHHHHEECCCCEEEEEEC
PVTPRMLRAQLETLKADLYPSAVKIGLIANQPQLDFLADWLGENLPGVPVILDPVMVASC
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCEEECCHHHHHC
GGDLQSPDNTCPDKTGERSGLDFSPFAGKISLITPNKAELSRLIGRLLNDDDALEWAANV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHH
LWQAFGGSILAKGGDCPWQQSKARDLLLLGDIATISPLHRRQGFWLSNERVATVHNHGSG
HHHHHCCCEEECCCCCCCCCCCCCCEEEECCHHHHCHHHHHCCCEECCCEEEEEECCCCC
CTLSSAIAAFVAKGLVLPDAVLLAKAYVFEGLRQASAVGSGPGPLARTGMPESLAAMASI
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
HRLHESRSHRLPTKGFTRLGHDLGVYPVVADIDMLETLLEAGANPIQLRIKGEIGPGSAA
HHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
EADIIRAIALGRQHRARVFINDHWRLAISHGAFGVHLGQEDIEAVPLDAIAEAGMALGLS
HHHHHHHHHHCCCCCEEEEEECCEEEEEECCCEEEECCHHHHHHCCHHHHHHCCHHCCCC
SHGLFEALLALELNPSYLALGHIFPTTTKEMPSKPQGLETLALLAKIVSTAVPTVAIGGI
CCHHHHHHHHEECCCCEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCEEEECCC
EAGNLPEVAATGVDSIAVVRAVTEASEPATAFRALADAWEMRNAR
CCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA