Click here to switch to the map view.

The map label for this gene is thiF [H]

Identifier: 119774993

GI number: 119774993

Start: 2261431

End: 2262183

Strand: Reverse

Name: thiF [H]

Synonym: Sama_1858

Alternate gene names: 119774993

Gene position: 2262183-2261431 (Counterclockwise)

Preceding gene: 119774994

Following gene: 119774992

Centisome position: 52.53

GC content: 57.77

Gene sequence:

>753_bases
ATGCTCGCTGATAAAGATTTTATCCGCTACTCGCGGCAAATTTTGCTGCCAGAATGCGGCGAAGCCGGGCAGCTCAGATT
AAGGAACTCCCATGTGGCCATAGTGGGCGTGGGTGGCCTTGGCTGTCAGGCGGCCATGCTGCTGGCCGCGTCGGGAGTGG
GGCACTTATCACTGTTCGATGCCGATAGCGTGGAGCTTTCTAACCTGCCGCGCCAGATGCTGTTTTGCGACCTTGATTTG
GGCAAAACCAAGGCCGGGGTCGCCGCCGCGCGCCTTCAGGCTCGAGAGCCTGGCTTAAAAGTCTCTGTGTACGGCGAGCT
TAACAGTGAGACGTTAACTCAGCTCGACGGCGCCGGGCTGGTGCTTGATTGCACCGATAATTTTACGGCGCGGCATCTTA
TTTCGGCGTACTGCGCTAAACACGGCAAAACGCTTATCAGCGGTGCCATTGCCGGTTTTGACGGGCTTTTGTTTGTTCAG
CGGCCCGGTGAGGGTGGCTGCTATCAGTGTCTGTTCCCGCCAGGCACCACTTCGGCGCAAAACTGTGCTGCGGTTGGGGT
TATGGGGCCCGCTGTGGCCATTGTCGCTTCGATGCAGGCACAACTTTGTTTGAATGAACTGCTTCAACTTCCCTGCGAGG
CTGGACGCCTGCAACGTTTCGATTTCAGGCATTTTCGGTGGCATGAAGCCAGGGTGCCTGCCGATCCCCTTTGCCCGGTT
TGCGGTAAGGGCAAGGAGCGCAAGGCATGCTGA

Upstream 100 bases:

>100_bases
GCTACAGGCGTGGACAGCATTGCCGTGGTACGTGCTGTGACCGAAGCAAGCGAACCGGCGACTGCCTTTCGCGCACTCGC
TGACGCCTGGGAGATGCGGA

Downstream 100 bases:

>100_bases
GTATCAGTGTTAACGGACATTCCCATCCAATAGACGACGGCCAGGATCTGGCCAGTTTGGTTAAACATCACTGCGAGCGC
GATAGCCATATCGCGCTGGT

Product: thiF protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MLADKDFIRYSRQILLPECGEAGQLRLRNSHVAIVGVGGLGCQAAMLLAASGVGHLSLFDADSVELSNLPRQMLFCDLDL
GKTKAGVAAARLQAREPGLKVSVYGELNSETLTQLDGAGLVLDCTDNFTARHLISAYCAKHGKTLISGAIAGFDGLLFVQ
RPGEGGCYQCLFPPGTTSAQNCAAVGVMGPAVAIVASMQAQLCLNELLQLPCEAGRLQRFDFRHFRWHEARVPADPLCPV
CGKGKERKAC

Sequences:

>Translated_250_residues
MLADKDFIRYSRQILLPECGEAGQLRLRNSHVAIVGVGGLGCQAAMLLAASGVGHLSLFDADSVELSNLPRQMLFCDLDL
GKTKAGVAAARLQAREPGLKVSVYGELNSETLTQLDGAGLVLDCTDNFTARHLISAYCAKHGKTLISGAIAGFDGLLFVQ
RPGEGGCYQCLFPPGTTSAQNCAAVGVMGPAVAIVASMQAQLCLNELLQLPCEAGRLQRFDFRHFRWHEARVPADPLCPV
CGKGKERKAC
>Mature_250_residues
MLADKDFIRYSRQILLPECGEAGQLRLRNSHVAIVGVGGLGCQAAMLLAASGVGHLSLFDADSVELSNLPRQMLFCDLDL
GKTKAGVAAARLQAREPGLKVSVYGELNSETLTQLDGAGLVLDCTDNFTARHLISAYCAKHGKTLISGAIAGFDGLLFVQ
RPGEGGCYQCLFPPGTTSAQNCAAVGVMGPAVAIVASMQAQLCLNELLQLPCEAGRLQRFDFRHFRWHEARVPADPLCPV
CGKGKERKAC

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=186, Percent_Identity=40.8602150537634, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI4885649, Length=183, Percent_Identity=28.9617486338798, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI38045942, Length=189, Percent_Identity=31.7460317460317, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI38045944, Length=189, Percent_Identity=31.7460317460317, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI38045948, Length=164, Percent_Identity=32.9268292682927, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87082356, Length=244, Percent_Identity=40.1639344262295, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787048, Length=247, Percent_Identity=36.0323886639676, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1789177, Length=136, Percent_Identity=30.1470588235294, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=254, Percent_Identity=38.1889763779528, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71984476, Length=175, Percent_Identity=32.5714285714286, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193203301, Length=197, Percent_Identity=24.8730964467005, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=213, Percent_Identity=35.6807511737089, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320598, Length=155, Percent_Identity=27.741935483871, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325323, Length=149, Percent_Identity=30.8724832214765, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322639, Length=163, Percent_Identity=31.9018404907975, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582879, Length=251, Percent_Identity=39.0438247011952, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24660640, Length=150, Percent_Identity=32.6666666666667, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129973, Length=161, Percent_Identity=33.5403726708075, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24641311, Length=165, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 26617; Mature: 26617

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLADKDFIRYSRQILLPECGEAGQLRLRNSHVAIVGVGGLGCQAAMLLAASGVGHLSLFD
CCCCHHHHHHHHHEECCCCCCCCCEEEECCEEEEEEECCCCHHHHHHHHHCCCCEEEEEC
ADSVELSNLPRQMLFCDLDLGKTKAGVAAARLQAREPGLKVSVYGELNSETLTQLDGAGL
CCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCE
VLDCTDNFTARHLISAYCAKHGKTLISGAIAGFDGLLFVQRPGEGGCYQCLFPPGTTSAQ
EEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCC
NCAAVGVMGPAVAIVASMQAQLCLNELLQLPCEAGRLQRFDFRHFRWHEARVPADPLCPV
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEECCCCCCCCCCCC
CGKGKERKAC
CCCCCCCCCC
>Mature Secondary Structure
MLADKDFIRYSRQILLPECGEAGQLRLRNSHVAIVGVGGLGCQAAMLLAASGVGHLSLFD
CCCCHHHHHHHHHEECCCCCCCCCEEEECCEEEEEEECCCCHHHHHHHHHCCCCEEEEEC
ADSVELSNLPRQMLFCDLDLGKTKAGVAAARLQAREPGLKVSVYGELNSETLTQLDGAGL
CCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCE
VLDCTDNFTARHLISAYCAKHGKTLISGAIAGFDGLLFVQRPGEGGCYQCLFPPGTTSAQ
EEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCC
NCAAVGVMGPAVAIVASMQAQLCLNELLQLPCEAGRLQRFDFRHFRWHEARVPADPLCPV
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEECCCCCCCCCCCC
CGKGKERKAC
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]