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The map label for this gene is thiC

Identifier: 119774995

GI number: 119774995

Start: 2263751

End: 2265745

Strand: Reverse

Name: thiC

Synonym: Sama_1860

Alternate gene names: 119774995

Gene position: 2265745-2263751 (Counterclockwise)

Preceding gene: 119774996

Following gene: 119774994

Centisome position: 52.62

GC content: 56.59

Gene sequence:

>1995_bases
ATGTCAAACCGCCGCGAAACCCGTAAAGCCGCCCAGGACTTTATCGATAACTTAAAGCCGCTGCGCCGCCCCAATTCCGA
AAAGATTTTCATTGAAGGCTCACGGCCTGATATCCAGGTGGGCATGCGCCAAATCCATCAAACCGATACCCGTATCGTGA
ATACCCGCGGCGAAGAAACCTTTGAGGCCAACCCACCCATCAAGGTGTACGACTGCGCCGGTCCTTATTCAGACCCCAAT
GCCGACATCAATGTGCGCCGAGGCCTGCCCAAGCTGCGTGAAGGCTGGATTTTAGCGCGTAGCGATACCGAAGAGCTTAA
CAGCGCCTCATCCAACTTTACCCAGCAGCGTCTCGCCGATGACGGGCTGGATCACCTGCGTTTTGATGTGCTGCCAAAGC
CGCGCCGCGCCAAGGCCGGTAAGCGGGTTACCCAGCTGCATTATGCCCGTCAGGGTATTATCACCCCTGAGATGGAATAC
ATCGCCATTCGCGAAAACATGGCCCGGGATGAAGTCACCGATGAGCAGCTTAACCGCAAAAAACCAGGCGAAGCCTTTGG
CGCTCTGGTGAGCAAACCCATTACCCCTGAGTTTGTCCGTGACGAAGTAGCCCGTGGCCGGGCGATTATTCCGGCCAACA
TCAATCACCCCGAAGCGGAGCCTATGATCATTGGTCGCAACTTTTTGGTGAAGGTGAACGCCAATATCGGCAACTCGGCC
GTGACCTCATCCATCGAAGAAGAGGTGGAAAAGCTGGTGTGGTCCACCCGCTGGGGCGCAGATACCGTGATGGACCTGTC
CACCGGCCGTTATATTCACGAAACCCGCGAATGGATTGTGCGTAACTCACCGGTGCCTATTGGCACTGTGCCGATTTATC
AGGCGCTGGAGAAGGTCAACGGCATCGCCGAAGATCTTAACTGGGAAGTATTCCGCGACACCCTGATTGAACAGGCCGAG
CAGGGGGTGGATTACTTCACCATCCACGCGGGCGTGCTGCTTCGCTACGTGCCCATGACTGCAAAACGGGTTACCGGCAT
AGTGTCCCGCGGCGGCTCCATCATGGCCAAATGGTGCTTAAGCCACCATCAGGAAAACTTCCTCTACACCCATTTTCGCG
ATATTTGCGAGCTTTGCGCCGCCTATGATGTGTCGTTGTCGCTCGGCGACGGTATGCGTCCGGGCTCCATTGCCGATGCC
AATGACGAAGCCCAGTTTGCTGAGCTCGAAACCCTGGGGGAGCTGGTAAAAATCGCCTGGGAATACGATGTGCAGACCAT
CATTGAAGGTCCGGGCCACATCCCCATGCAGCTCATCAAAGAGAACATGGATAAGCAGCTTGAGCACTGCGCAGAGGCGC
CGTTTTATACCCTGGGCCCGCAAACCACCGACATTGCCCCAGGCTACGATCACTTCACCTCCGGTATTGGGGCGGCCATG
ATTGCCTGGTATGGCTGCGCCATGCTTTGTTACGTCACGCCGAAAGAACATTTGGGCCTGCCGAACAAGGAAGATGTAAA
ACAAGGGCTTATTGCTTACAAGATTGCCGCCCACGCTGCCGATGTCGCCAAGGGACATCCCGGCGCGCAAATCCGCGACA
ATGCGCTGTCCAAGGCACGATTCGAGTTTCGCTGGGAGGATCAGTACAACCTCGGGCTGGATCCCGATACTGCCCGTGCC
TATCACGACGAAACTCTGCCGCAGGAGTCGGCCAAGGTGGCCCATTTTTGCTCCATGTGCGGCCCCAAGTTCTGCTCGAT
GAAAATCACCCAGGATGTACGCGACTATGCGGCCAGTCTGGAGAATGCTGCCCGCAGCGAGGCTGACAGCGTACAGACCC
TTGAAGTGGGGGCGGCCGAAAGCTATTCCAAGGCAGAAAGCTACGCCAGGGCGGGTATGGACAAGATGTCGGCCACCTTT
AAACAGACAGGCAGTGCGCTTTACAGCGAGACTCATGTGGAAGCGGCCCTGAGCACCGAAAAGGAACCGGTGTAG

Upstream 100 bases:

>100_bases
TAAACATCGCACCTTGGAATTTATCGTCGCCGCTGATTCAACTGCTCAATTTTCTCCCCCGACAAGCAACTCCATTTTGA
AACCAAAAGTGAGATTGCTG

Downstream 100 bases:

>100_bases
ATGGCCAAAATCAACGACATGGCAGCTAAACCCATCGTCTGGACTATCGCAGGCTCAGACAGCGGCGGCGGCGCCGGTAT
TCAGGCGGATTTGCTGACGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MSNRRETRKAAQDFIDNLKPLRRPNSEKIFIEGSRPDIQVGMRQIHQTDTRIVNTRGEETFEANPPIKVYDCAGPYSDPN
ADINVRRGLPKLREGWILARSDTEELNSASSNFTQQRLADDGLDHLRFDVLPKPRRAKAGKRVTQLHYARQGIITPEMEY
IAIRENMARDEVTDEQLNRKKPGEAFGALVSKPITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKVNANIGNSA
VTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIVRNSPVPIGTVPIYQALEKVNGIAEDLNWEVFRDTLIEQAE
QGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFRDICELCAAYDVSLSLGDGMRPGSIADA
NDEAQFAELETLGELVKIAWEYDVQTIIEGPGHIPMQLIKENMDKQLEHCAEAPFYTLGPQTTDIAPGYDHFTSGIGAAM
IAWYGCAMLCYVTPKEHLGLPNKEDVKQGLIAYKIAAHAADVAKGHPGAQIRDNALSKARFEFRWEDQYNLGLDPDTARA
YHDETLPQESAKVAHFCSMCGPKFCSMKITQDVRDYAASLENAARSEADSVQTLEVGAAESYSKAESYARAGMDKMSATF
KQTGSALYSETHVEAALSTEKEPV

Sequences:

>Translated_664_residues
MSNRRETRKAAQDFIDNLKPLRRPNSEKIFIEGSRPDIQVGMRQIHQTDTRIVNTRGEETFEANPPIKVYDCAGPYSDPN
ADINVRRGLPKLREGWILARSDTEELNSASSNFTQQRLADDGLDHLRFDVLPKPRRAKAGKRVTQLHYARQGIITPEMEY
IAIRENMARDEVTDEQLNRKKPGEAFGALVSKPITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKVNANIGNSA
VTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIVRNSPVPIGTVPIYQALEKVNGIAEDLNWEVFRDTLIEQAE
QGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFRDICELCAAYDVSLSLGDGMRPGSIADA
NDEAQFAELETLGELVKIAWEYDVQTIIEGPGHIPMQLIKENMDKQLEHCAEAPFYTLGPQTTDIAPGYDHFTSGIGAAM
IAWYGCAMLCYVTPKEHLGLPNKEDVKQGLIAYKIAAHAADVAKGHPGAQIRDNALSKARFEFRWEDQYNLGLDPDTARA
YHDETLPQESAKVAHFCSMCGPKFCSMKITQDVRDYAASLENAARSEADSVQTLEVGAAESYSKAESYARAGMDKMSATF
KQTGSALYSETHVEAALSTEKEPV
>Mature_663_residues
SNRRETRKAAQDFIDNLKPLRRPNSEKIFIEGSRPDIQVGMRQIHQTDTRIVNTRGEETFEANPPIKVYDCAGPYSDPNA
DINVRRGLPKLREGWILARSDTEELNSASSNFTQQRLADDGLDHLRFDVLPKPRRAKAGKRVTQLHYARQGIITPEMEYI
AIRENMARDEVTDEQLNRKKPGEAFGALVSKPITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKVNANIGNSAV
TSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIVRNSPVPIGTVPIYQALEKVNGIAEDLNWEVFRDTLIEQAEQ
GVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFRDICELCAAYDVSLSLGDGMRPGSIADAN
DEAQFAELETLGELVKIAWEYDVQTIIEGPGHIPMQLIKENMDKQLEHCAEAPFYTLGPQTTDIAPGYDHFTSGIGAAMI
AWYGCAMLCYVTPKEHLGLPNKEDVKQGLIAYKIAAHAADVAKGHPGAQIRDNALSKARFEFRWEDQYNLGLDPDTARAY
HDETLPQESAKVAHFCSMCGPKFCSMKITQDVRDYAASLENAARSEADSVQTLEVGAAESYSKAESYARAGMDKMSATFK
QTGSALYSETHVEAALSTEKEPV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=646, Percent_Identity=74.6130030959752, Blast_Score=1010, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_SHEAM (A1S6Q9)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927735.1
- STRING:   A1S6Q9
- GeneID:   4604110
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1860
- NMPDR:   fig|326297.7.peg.1750
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   A1S6Q9
- ProtClustDB:   PRK09284
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 74154; Mature: 74023

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: BINDING 235-235 BINDING 264-264 BINDING 293-293 BINDING 329-329 BINDING 429-429 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRRETRKAAQDFIDNLKPLRRPNSEKIFIEGSRPDIQVGMRQIHQTDTRIVNTRGEET
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEECCCCCC
FEANPPIKVYDCAGPYSDPNADINVRRGLPKLREGWILARSDTEELNSASSNFTQQRLAD
CCCCCCEEEEECCCCCCCCCCCEEHHCCCCHHHHCEEEEECCHHHHHHHHHHHHHHHHHH
DGLDHLRFDVLPKPRRAKAGKRVTQLHYARQGIITPEMEYIAIRENMARDEVTDEQLNRK
CCHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCC
KPGEAFGALVSKPITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKVNANIGNSA
CCHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCCHH
VTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIVRNSPVPIGTVPIYQALEKVN
HHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GIAEDLNWEVFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCL
HHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SHHQENFLYTHFRDICELCAAYDVSLSLGDGMRPGSIADANDEAQFAELETLGELVKIAW
HHHCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EYDVQTIIEGPGHIPMQLIKENMDKQLEHCAEAPFYTLGPQTTDIAPGYDHFTSGIGAAM
HHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHH
IAWYGCAMLCYVTPKEHLGLPNKEDVKQGLIAYKIAAHAADVAKGHPGAQIRDNALSKAR
HHHHHHHHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCE
FEFRWEDQYNLGLDPDTARAYHDETLPQESAKVAHFCSMCGPKFCSMKITQDVRDYAASL
EEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHH
ENAARSEADSVQTLEVGAAESYSKAESYARAGMDKMSATFKQTGSALYSETHVEAALSTE
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEPV
CCCC
>Mature Secondary Structure 
SNRRETRKAAQDFIDNLKPLRRPNSEKIFIEGSRPDIQVGMRQIHQTDTRIVNTRGEET
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEECCCCCC
FEANPPIKVYDCAGPYSDPNADINVRRGLPKLREGWILARSDTEELNSASSNFTQQRLAD
CCCCCCEEEEECCCCCCCCCCCEEHHCCCCHHHHCEEEEECCHHHHHHHHHHHHHHHHHH
DGLDHLRFDVLPKPRRAKAGKRVTQLHYARQGIITPEMEYIAIRENMARDEVTDEQLNRK
CCHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCC
KPGEAFGALVSKPITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKVNANIGNSA
CCHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCCHH
VTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIVRNSPVPIGTVPIYQALEKVN
HHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GIAEDLNWEVFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCL
HHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SHHQENFLYTHFRDICELCAAYDVSLSLGDGMRPGSIADANDEAQFAELETLGELVKIAW
HHHCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EYDVQTIIEGPGHIPMQLIKENMDKQLEHCAEAPFYTLGPQTTDIAPGYDHFTSGIGAAM
HHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHH
IAWYGCAMLCYVTPKEHLGLPNKEDVKQGLIAYKIAAHAADVAKGHPGAQIRDNALSKAR
HHHHHHHHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCE
FEFRWEDQYNLGLDPDTARAYHDETLPQESAKVAHFCSMCGPKFCSMKITQDVRDYAASL
EEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHH
ENAARSEADSVQTLEVGAAESYSKAESYARAGMDKMSATFKQTGSALYSETHVEAALSTE
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEPV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA