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The map label for this gene is eptA [H]

Identifier: 119774893

GI number: 119774893

Start: 2150332

End: 2151948

Strand: Direct

Name: eptA [H]

Synonym: Sama_1758

Alternate gene names: 119774893

Gene position: 2150332-2151948 (Clockwise)

Preceding gene: 119774892

Following gene: 119774894

Centisome position: 49.94

GC content: 47.74

Gene sequence:

>1617_bases
TTGAAGTCCATTGGTTTGAATCGGTTCACTTTTTTTATAGCCCTGTATTACGCAGCGATCCTGAACATTCCCCTGTATAT
CAGGGCGGCAGAAGGGCTGAAGTCATTGTCACACATCGATTGGTTGTTTGTAGCAAGCTTACCTGTACTGCTGACATCCT
TACTGGCACTCATCTTCTCGCTTTTCAGTGTTAAGTACCTCGTAAAACCTTTTTTTATCCTGCTGACGTTGGTTTCGGCG
TTGGTCGTCTTTGGCATGTATAAATACGGTATCGTGTTTGACCGTGCCATGATGGAAAACATCTTTGAAACCCACAGCGC
CGAAGCGTTCATGTATCTCAATACAGAGTCCGTGGTTGGCTTTTTACTGCTTGGGATACTGCCGGCGTTTCTTATTGCCA
AAGTTAAAATTGAATATCGGGTCTGGTGGAAAGAGCTGCTGGTTAAGTCTGGCTTCATGCTGGGAATGGTATCGATTGCC
GGTGGTGTGCTTGCCGGTTACTACCAGGACTATGTCGCCTTTGGGCGAAATAATGATGATATGAAACAACTGGTTGTACC
TACCTATTACATAGGCGCGGCGGCAGGGTTCGTGAATCAGCGTTATATTGAAACGCCGTTGGAATATCAGCCCCTTGGGA
CAGATGCCAACAACCAAACCCGGGACAGCAATCAAAAACCCCTGTTAACCGTGCTGTTGGTCGGGGAAACCGCCCGTTCA
ATGAATTATCAGTATTATGGCTATCAAAAAGATACCAATGCGTTTACCAAGCCCCACAATCCCATTGTACTGAGCGCGAC
CCGGTCATGTGGTACCGCAACAGCACAATCCTTACCCTGTATGTTCTCCCGTATGGGCAGGGAAGACTACAATGCGAGAA
AAGCCAGAGCCCAGGATACCGTAATCGATGTACTCGATCATGCCGGTGTGACTGTGTATTGGATTGATAGTGACAGTGGT
TGTAAGGGTGTTTGTGATCGGGTAATCAACGAAACAGTCAAGCTGGATGAGGATCCCGCTCTGTGTGATGGCGAGTCTTG
TCTGGATCAAGTCCTTTTGAATCGTCTCGATAACATACTGGCGAGTCCCATTGAAAAAGACACCCTATTGGTGCTGCATA
TTATGGGCAGCCATGGACCTACGTACTTCCAGCGCTATCCGGACGAACATAAAGCCTTCACGCCAGATTGCCCACGCAGT
GATATTCAAAACTGCAGTAACGAGGCGCTCGTAAATACCTACGACAACACCATCCGTTACAGTGACTACATCATGGCGGA
GGTGATTAATAGACTGAAACAGCAAGAGCCAGTGGCCGATACTGCGATGATGTATATCTCAGATCATGGAGAGTCTCTGG
GCGAAAAGGGCTTGTACCTGCATGGTGCGCCCTATGCATTTGCACCCATCGAGCAAATCAGTGTGCCCTGGCTGTTGTGG
CTATCTGACGGTTTTAAAGAAGAAAACCGCCTGGATGAAAGCTGTTTAAGGAGCTTGCAGGGCGAATGGTCCCATGACAA
TCTATTTGATAGCTTGTTGGGGTGGATGAATATTCACACCAGCATATATCGCAAGGAGCTGGATATTTTCAGTCGCTGTC
GACTTCCGGCGGTATAA

Upstream 100 bases:

>100_bases
ATTGGGCATTTGCCCTATAGACTGAGACTTTTGTTTTATTTTCTAATGCCACGTTTACAATAAGCATACTTTTAAAGGGC
TTTTTTGGGGGAAAGTTAGT

Downstream 100 bases:

>100_bases
CCAGCGCAATTAGCGTGACTTAACCCAAGATTCAGGCAAACCCCGTGGTCAACGAGCCACGGGCAGTTAAGGATGAAGTA
TTTGCCATGACACTCGCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MKSIGLNRFTFFIALYYAAILNIPLYIRAAEGLKSLSHIDWLFVASLPVLLTSLLALIFSLFSVKYLVKPFFILLTLVSA
LVVFGMYKYGIVFDRAMMENIFETHSAEAFMYLNTESVVGFLLLGILPAFLIAKVKIEYRVWWKELLVKSGFMLGMVSIA
GGVLAGYYQDYVAFGRNNDDMKQLVVPTYYIGAAAGFVNQRYIETPLEYQPLGTDANNQTRDSNQKPLLTVLLVGETARS
MNYQYYGYQKDTNAFTKPHNPIVLSATRSCGTATAQSLPCMFSRMGREDYNARKARAQDTVIDVLDHAGVTVYWIDSDSG
CKGVCDRVINETVKLDEDPALCDGESCLDQVLLNRLDNILASPIEKDTLLVLHIMGSHGPTYFQRYPDEHKAFTPDCPRS
DIQNCSNEALVNTYDNTIRYSDYIMAEVINRLKQQEPVADTAMMYISDHGESLGEKGLYLHGAPYAFAPIEQISVPWLLW
LSDGFKEENRLDESCLRSLQGEWSHDNLFDSLLGWMNIHTSIYRKELDIFSRCRLPAV

Sequences:

>Translated_538_residues
MKSIGLNRFTFFIALYYAAILNIPLYIRAAEGLKSLSHIDWLFVASLPVLLTSLLALIFSLFSVKYLVKPFFILLTLVSA
LVVFGMYKYGIVFDRAMMENIFETHSAEAFMYLNTESVVGFLLLGILPAFLIAKVKIEYRVWWKELLVKSGFMLGMVSIA
GGVLAGYYQDYVAFGRNNDDMKQLVVPTYYIGAAAGFVNQRYIETPLEYQPLGTDANNQTRDSNQKPLLTVLLVGETARS
MNYQYYGYQKDTNAFTKPHNPIVLSATRSCGTATAQSLPCMFSRMGREDYNARKARAQDTVIDVLDHAGVTVYWIDSDSG
CKGVCDRVINETVKLDEDPALCDGESCLDQVLLNRLDNILASPIEKDTLLVLHIMGSHGPTYFQRYPDEHKAFTPDCPRS
DIQNCSNEALVNTYDNTIRYSDYIMAEVINRLKQQEPVADTAMMYISDHGESLGEKGLYLHGAPYAFAPIEQISVPWLLW
LSDGFKEENRLDESCLRSLQGEWSHDNLFDSLLGWMNIHTSIYRKELDIFSRCRLPAV
>Mature_538_residues
MKSIGLNRFTFFIALYYAAILNIPLYIRAAEGLKSLSHIDWLFVASLPVLLTSLLALIFSLFSVKYLVKPFFILLTLVSA
LVVFGMYKYGIVFDRAMMENIFETHSAEAFMYLNTESVVGFLLLGILPAFLIAKVKIEYRVWWKELLVKSGFMLGMVSIA
GGVLAGYYQDYVAFGRNNDDMKQLVVPTYYIGAAAGFVNQRYIETPLEYQPLGTDANNQTRDSNQKPLLTVLLVGETARS
MNYQYYGYQKDTNAFTKPHNPIVLSATRSCGTATAQSLPCMFSRMGREDYNARKARAQDTVIDVLDHAGVTVYWIDSDSG
CKGVCDRVINETVKLDEDPALCDGESCLDQVLLNRLDNILASPIEKDTLLVLHIMGSHGPTYFQRYPDEHKAFTPDCPRS
DIQNCSNEALVNTYDNTIRYSDYIMAEVINRLKQQEPVADTAMMYISDHGESLGEKGLYLHGAPYAFAPIEQISVPWLLW
LSDGFKEENRLDESCLRSLQGEWSHDNLFDSLLGWMNIHTSIYRKELDIFSRCRLPAV

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=525, Percent_Identity=38.0952380952381, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI87082286, Length=298, Percent_Identity=27.8523489932886, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87082223, Length=447, Percent_Identity=22.5950782997763, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 60833; Mature: 60833

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIGLNRFTFFIALYYAAILNIPLYIRAAEGLKSLSHIDWLFVASLPVLLTSLLALIFS
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFSVKYLVKPFFILLTLVSALVVFGMYKYGIVFDRAMMENIFETHSAEAFMYLNTESVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
FLLLGILPAFLIAKVKIEYRVWWKELLVKSGFMLGMVSIAGGVLAGYYQDYVAFGRNNDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MKQLVVPTYYIGAAAGFVNQRYIETPLEYQPLGTDANNQTRDSNQKPLLTVLLVGETARS
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHH
MNYQYYGYQKDTNAFTKPHNPIVLSATRSCGTATAQSLPCMFSRMGREDYNARKARAQDT
CCCEEECEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VIDVLDHAGVTVYWIDSDSGCKGVCDRVINETVKLDEDPALCDGESCLDQVLLNRLDNIL
HHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
ASPIEKDTLLVLHIMGSHGPTYFQRYPDEHKAFTPDCPRSDIQNCSNEALVNTYDNTIRY
HCCCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCCEEH
SDYIMAEVINRLKQQEPVADTAMMYISDHGESLGEKGLYLHGAPYAFAPIEQISVPWLLW
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCEEEECCCCCCCCHHHCCCCEEEE
LSDGFKEENRLDESCLRSLQGEWSHDNLFDSLLGWMNIHTSIYRKELDIFSRCRLPAV
ECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSIGLNRFTFFIALYYAAILNIPLYIRAAEGLKSLSHIDWLFVASLPVLLTSLLALIFS
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFSVKYLVKPFFILLTLVSALVVFGMYKYGIVFDRAMMENIFETHSAEAFMYLNTESVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
FLLLGILPAFLIAKVKIEYRVWWKELLVKSGFMLGMVSIAGGVLAGYYQDYVAFGRNNDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MKQLVVPTYYIGAAAGFVNQRYIETPLEYQPLGTDANNQTRDSNQKPLLTVLLVGETARS
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHH
MNYQYYGYQKDTNAFTKPHNPIVLSATRSCGTATAQSLPCMFSRMGREDYNARKARAQDT
CCCEEECEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VIDVLDHAGVTVYWIDSDSGCKGVCDRVINETVKLDEDPALCDGESCLDQVLLNRLDNIL
HHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
ASPIEKDTLLVLHIMGSHGPTYFQRYPDEHKAFTPDCPRSDIQNCSNEALVNTYDNTIRY
HCCCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCCEEH
SDYIMAEVINRLKQQEPVADTAMMYISDHGESLGEKGLYLHGAPYAFAPIEQISVPWLLW
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCEEEECCCCCCCCHHHCCCCEEEE
LSDGFKEENRLDESCLRSLQGEWSHDNLFDSLLGWMNIHTSIYRKELDIFSRCRLPAV
ECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]