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The map label for this gene is pleD [H]

Identifier: 119774894

GI number: 119774894

Start: 2151993

End: 2153060

Strand: Direct

Name: pleD [H]

Synonym: Sama_1759

Alternate gene names: 119774894

Gene position: 2151993-2153060 (Clockwise)

Preceding gene: 119774893

Following gene: 119774897

Centisome position: 49.97

GC content: 50.09

Gene sequence:

>1068_bases
GTGGTCAACGAGCCACGGGCAGTTAAGGATGAAGTATTTGCCATGACACTCGCAGCTCACAAACCCAAAGAAAAAGATGA
GTCGGCCGGGATATTGAGAAAGGCCGTACCCCAAATGTCGGCACTCGGCATTCCCGTAACACCCGACAATTATGCCGTTT
GGTATGCCTACTATCAGGGGGCCGATTTGGCGCTTGTCAGGGCTATCAATGGTTTGATGGAAAATCAGGTCTCTTTTTCT
GCGGAGGTGTGCCGGGGACTCTACAGCAGTTTTATCCAGAATAATGCACCCGAAGTATTGGAAAACGTGCACCTGGAAAC
CCAGTTGCTGATCAATGGGCTACTCAGCAAAATCAGTCTGTTGACCTCAGGTTCTGAGACATTTCAGCTCGCCATAGAAA
GTTTTGGCCAGCAATTGCGGCTTCCTCAGGAAGCGGAATCGCTGCATCGATTGGTGGAGGGCGTGTTGGTTGAGGTGGAC
AAGGTGGTAAGTGAGAACGCGGATATCCACTCAAGTCTCAAGTCGATGGATCAGGAACTCAATCATCTACGAGCTGAAAT
AACCGAGTTGTCGGTGATATCCCTTACCGATAAATTAACCGGGCTGAATAATCGCCGTGCATTTGAATCTCGTTTGGCGC
AGGAGTTTTTACCATCAACGCCAAAATGTTCGCTGTTGGTTATCGATATTGATCACTTTAAGCAGTTTAACGATACATGG
GGCCACAATGCCGGAGACAAGGTGTTGGTGTATGTGGCGCAGAATCTGAAACAGGCCGTAAAGGGCGAGGATTTGGTTGC
CCGCTACGGAGGCGAAGAATTTGTCATTGTGTTGAAGGATACCAGCGCTGAGGATGCAGCCAACATTGCGGAAAAGCTGC
GCGACCGTATCGCCAACAAAAAATTGACCTTGAAGAAAGACAATTTGGATTTGGGTAAGGTATCCGTATCCATCGGCGTC
GCCAAGGCCTATGCCAATGAAACCCCTGAACAGTTTTTTGAGAGGGCGGACGGCGCGCTTTACCGCGCCAAAGGCACTGG
CCGGAACCGGGTTTGCCTCGCTGACTAG

Upstream 100 bases:

>100_bases
GCATATATCGCAAGGAGCTGGATATTTTCAGTCGCTGTCGACTTCCGGCGGTATAACCAGCGCAATTAGCGTGACTTAAC
CCAAGATTCAGGCAAACCCC

Downstream 100 bases:

>100_bases
CGACCGTTATCGGTCGCTGGTCGTTCCCCTGTGATCAGATGACTGTATTCGGAACGCGGTTTTCAGCAGTCAGGTTGCAT
GCAGGTCGTTATCAACACCA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MVNEPRAVKDEVFAMTLAAHKPKEKDESAGILRKAVPQMSALGIPVTPDNYAVWYAYYQGADLALVRAINGLMENQVSFS
AEVCRGLYSSFIQNNAPEVLENVHLETQLLINGLLSKISLLTSGSETFQLAIESFGQQLRLPQEAESLHRLVEGVLVEVD
KVVSENADIHSSLKSMDQELNHLRAEITELSVISLTDKLTGLNNRRAFESRLAQEFLPSTPKCSLLVIDIDHFKQFNDTW
GHNAGDKVLVYVAQNLKQAVKGEDLVARYGGEEFVIVLKDTSAEDAANIAEKLRDRIANKKLTLKKDNLDLGKVSVSIGV
AKAYANETPEQFFERADGALYRAKGTGRNRVCLAD

Sequences:

>Translated_355_residues
MVNEPRAVKDEVFAMTLAAHKPKEKDESAGILRKAVPQMSALGIPVTPDNYAVWYAYYQGADLALVRAINGLMENQVSFS
AEVCRGLYSSFIQNNAPEVLENVHLETQLLINGLLSKISLLTSGSETFQLAIESFGQQLRLPQEAESLHRLVEGVLVEVD
KVVSENADIHSSLKSMDQELNHLRAEITELSVISLTDKLTGLNNRRAFESRLAQEFLPSTPKCSLLVIDIDHFKQFNDTW
GHNAGDKVLVYVAQNLKQAVKGEDLVARYGGEEFVIVLKDTSAEDAANIAEKLRDRIANKKLTLKKDNLDLGKVSVSIGV
AKAYANETPEQFFERADGALYRAKGTGRNRVCLAD
>Mature_355_residues
MVNEPRAVKDEVFAMTLAAHKPKEKDESAGILRKAVPQMSALGIPVTPDNYAVWYAYYQGADLALVRAINGLMENQVSFS
AEVCRGLYSSFIQNNAPEVLENVHLETQLLINGLLSKISLLTSGSETFQLAIESFGQQLRLPQEAESLHRLVEGVLVEVD
KVVSENADIHSSLKSMDQELNHLRAEITELSVISLTDKLTGLNNRRAFESRLAQEFLPSTPKCSLLVIDIDHFKQFNDTW
GHNAGDKVLVYVAQNLKQAVKGEDLVARYGGEEFVIVLKDTSAEDAANIAEKLRDRIANKKLTLKKDNLDLGKVSVSIGV
AKAYANETPEQFFERADGALYRAKGTGRNRVCLAD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=199, Percent_Identity=35.678391959799, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=42.2360248447205, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=43.4782608695652, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=36.2068965517241, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787816, Length=171, Percent_Identity=34.5029239766082, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=36.7469879518072, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787802, Length=157, Percent_Identity=35.6687898089172, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=34.0782122905028, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=31.055900621118, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788085, Length=197, Percent_Identity=28.9340101522843, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=30.5555555555556, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=30.9677419354839, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=28.0701754385965, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=30.0613496932515, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39231; Mature: 39231

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNEPRAVKDEVFAMTLAAHKPKEKDESAGILRKAVPQMSALGIPVTPDNYAVWYAYYQG
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECC
ADLALVRAINGLMENQVSFSAEVCRGLYSSFIQNNAPEVLENVHLETQLLINGLLSKISL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTSGSETFQLAIESFGQQLRLPQEAESLHRLVEGVLVEVDKVVSENADIHSSLKSMDQEL
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NHLRAEITELSVISLTDKLTGLNNRRAFESRLAQEFLPSTPKCSLLVIDIDHFKQFNDTW
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCC
GHNAGDKVLVYVAQNLKQAVKGEDLVARYGGEEFVIVLKDTSAEDAANIAEKLRDRIANK
CCCCCCCEEHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
KLTLKKDNLDLGKVSVSIGVAKAYANETPEQFFERADGALYRAKGTGRNRVCLAD
CEEEEECCCCCCEEEEHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCEEECC
>Mature Secondary Structure
MVNEPRAVKDEVFAMTLAAHKPKEKDESAGILRKAVPQMSALGIPVTPDNYAVWYAYYQG
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECC
ADLALVRAINGLMENQVSFSAEVCRGLYSSFIQNNAPEVLENVHLETQLLINGLLSKISL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTSGSETFQLAIESFGQQLRLPQEAESLHRLVEGVLVEVDKVVSENADIHSSLKSMDQEL
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NHLRAEITELSVISLTDKLTGLNNRRAFESRLAQEFLPSTPKCSLLVIDIDHFKQFNDTW
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCC
GHNAGDKVLVYVAQNLKQAVKGEDLVARYGGEEFVIVLKDTSAEDAANIAEKLRDRIANK
CCCCCCCEEHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
KLTLKKDNLDLGKVSVSIGVAKAYANETPEQFFERADGALYRAKGTGRNRVCLAD
CEEEEECCCCCCEEEEHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA