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The map label for this gene is ptrB [C]

Identifier: 119774892

GI number: 119774892

Start: 2148076

End: 2150166

Strand: Direct

Name: ptrB [C]

Synonym: Sama_1757

Alternate gene names: 119774892

Gene position: 2148076-2150166 (Clockwise)

Preceding gene: 119774891

Following gene: 119774893

Centisome position: 49.88

GC content: 51.89

Gene sequence:

>2091_bases
ATGAATAAACGGATACTGCTTCTTGGCGCAGCCTCATTGCTGGCTTTCAATGCTGTAGCACAAGGGGACCCCTTCATTTG
GCTGGAAGATGTCGAGGGTGAAAAAGCGCTTGCCTGGGTCAAAACCCAAAACGATCGCTCTCTTGCAGAGCTTAAAGCCG
TCCCAGGTTATAACGCGTTGGTGGACAATGCACTGGATATCCTTAATGACAAGGCGCGTATCCCGTACGCGAGTCGCATT
GGTGACCATCTCTATAACTTCTGGAAAGACGAAGCCAATCCCCGCGGCATTTACCGACGCACCACCATGGCGGAATACGT
TAAAGACGCGCCTAAATGGGAAACCGTGCTCGATATCGATGCACTTGGTAAATCCGAAGGGGTGAACTGGGTATTCAAGG
GCATGGACTGTCAGTATCCAAAGAATATACGTTGCCTGGTGTCCCTTTCCCGAGGCGGTGCCGATGCAGTGGAAATCCGC
GAATTTGACCTCTCAACCTTAAGTTTCGTGCCTGCCGACAAGCAGGGTTTTTTCTTGCCGGAGGCCAAGTCCAGCACAAG
TTGGATTGATGAAAACACCCTGTTTGTGGGTACCGATTTCGACGAAGGTGACGCCTGGACTGACTCTGGCTACCCGCGCA
AGGTAAAGGTTTGGAAGCGGGGCACTGATCTGAAAGACGCCAAAGAAATTTATGCAGGCAACAAGGCATCGGTGGCGGCA
TCCGGTTACGTCATGTGGGATGATAAAACGCCACTGCAATTGGTATCTGAAGCCGAAACCTTCTACGAAGCCAGTTACAA
GGCGCTCCTTGATGGCAAGCTGGTAGAGCTGCCGCTTCCCAAAGATGCCGAGCTCAAGGGCTTCTTCAAGGGCGATATTT
TTGTTGAGCTTAAGAGTAAGCTCGAGCAGGGCAACAACAGTTTCCCACAGGGCGCCATTGTCTACACCAAGGCAGACAAG
CTGCTGGCCGGAACGCCTGAGTTCGCACTCTTTGTAAAACCCGACGCCAATTCTTCCATCAGTCAGGTGACCTTTAGCCG
CAACGCGGTACTGGTGAACTGGCTCGAAGACGTGAAGAGCAAATTGGTGCGTTATCATAAAGATGCCAAAGGCCAGTGGC
AGGGCGAAGACGTTGGTTTCCCAAGCAATGGCAGCATCAGTGTGTTCGACAGCAGCCGCGACAGAGATGATCTGTTCGTA
ACCTATACCAGCTTCCTCGAACCTTCGACCCTGTACAGCGTTAACGCCGAAACCCTCAAGCGAGACTCGCTCAAGGCGAT
GCCTGCCCAGTTTGATGCGTCAAAGTTTGAGGCCAAGCAGTACTTTGCCACCAGTAAAGACGGTACCAAGGTGCCTTACT
TTGCGGTGATGGCCAAGGACATCAAACTCGACAGCACCAACCCAACCCTGCTGTATGGCTACGGTGGTTTTGAGGTATCG
CTGCGTCCTTTCTATTCTGCAACCACAGGTAAAAACTGGCTGGAGCAGGGCGGCGTTTATGTACTTGCCAACATTCGCGG
CGGCGGTGAATACGGTCCCGGCTGGCACCAGGCTGCATTGAAGGAAAACCGTCATAAAGCCTACGAAGACTTTGAAGCCA
TTGCTGAAGATCTGATTAAGCGCAAAATCACTTCCCCGAAGCATCTGGGTATTCAGGGTGGCAGTAATGGTGGCCTGCTG
ATGGGGGCTGCCTTTACCCGCAGACCCGATTTGTACAATGCCGTGGTATGTCAGGTACCGCTTCTGGATATGAAGCGTTA
CAACAAACTGTTGGCAGGCGCCAGCTGGATGGGCGAGTACGGCAACCCGGATATCGCCGCCGAATGGGATTACATTAAAA
CCTTCTCCCCATACCATAACCTTAAAAAAGACGTCGAATATCCCAAAGTATTTTTCACCACATCCACCCGCGATGACCGT
GTGCACCCAGGCCATGCCCGTAAAATGGTGGCCAAGATGGAAGACATGGGCATTGAGGTGCTGTACTTCGAAAACATGGA
AGGCGGCCATGCCGGCGCGGCAGATAATAAACAAACTGCGGAACTAAATAGCCTGGCATATGCCTACTTATTGAAGCAGC
TTAAGTTGTAA

Upstream 100 bases:

>100_bases
CTGTAACAGACGCTTACACTTCATTCACAGGTAAAGTTGCCAAGCGGCGGAAAATCGCTAGTTTAGTGTCCTTGAAAACG
TTAGTAACCAAGGACTCAAT

Downstream 100 bases:

>100_bases
CAGCCAAAATCCGGCTGAGACTTTAACAAAACCACCGAGGCTGCTGCCGGTCCGGTGGTTTTTCTATTGGGCATTTGCCC
TATAGACTGAGACTTTTGTT

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MNKRILLLGAASLLAFNAVAQGDPFIWLEDVEGEKALAWVKTQNDRSLAELKAVPGYNALVDNALDILNDKARIPYASRI
GDHLYNFWKDEANPRGIYRRTTMAEYVKDAPKWETVLDIDALGKSEGVNWVFKGMDCQYPKNIRCLVSLSRGGADAVEIR
EFDLSTLSFVPADKQGFFLPEAKSSTSWIDENTLFVGTDFDEGDAWTDSGYPRKVKVWKRGTDLKDAKEIYAGNKASVAA
SGYVMWDDKTPLQLVSEAETFYEASYKALLDGKLVELPLPKDAELKGFFKGDIFVELKSKLEQGNNSFPQGAIVYTKADK
LLAGTPEFALFVKPDANSSISQVTFSRNAVLVNWLEDVKSKLVRYHKDAKGQWQGEDVGFPSNGSISVFDSSRDRDDLFV
TYTSFLEPSTLYSVNAETLKRDSLKAMPAQFDASKFEAKQYFATSKDGTKVPYFAVMAKDIKLDSTNPTLLYGYGGFEVS
LRPFYSATTGKNWLEQGGVYVLANIRGGGEYGPGWHQAALKENRHKAYEDFEAIAEDLIKRKITSPKHLGIQGGSNGGLL
MGAAFTRRPDLYNAVVCQVPLLDMKRYNKLLAGASWMGEYGNPDIAAEWDYIKTFSPYHNLKKDVEYPKVFFTTSTRDDR
VHPGHARKMVAKMEDMGIEVLYFENMEGGHAGAADNKQTAELNSLAYAYLLKQLKL

Sequences:

>Translated_696_residues
MNKRILLLGAASLLAFNAVAQGDPFIWLEDVEGEKALAWVKTQNDRSLAELKAVPGYNALVDNALDILNDKARIPYASRI
GDHLYNFWKDEANPRGIYRRTTMAEYVKDAPKWETVLDIDALGKSEGVNWVFKGMDCQYPKNIRCLVSLSRGGADAVEIR
EFDLSTLSFVPADKQGFFLPEAKSSTSWIDENTLFVGTDFDEGDAWTDSGYPRKVKVWKRGTDLKDAKEIYAGNKASVAA
SGYVMWDDKTPLQLVSEAETFYEASYKALLDGKLVELPLPKDAELKGFFKGDIFVELKSKLEQGNNSFPQGAIVYTKADK
LLAGTPEFALFVKPDANSSISQVTFSRNAVLVNWLEDVKSKLVRYHKDAKGQWQGEDVGFPSNGSISVFDSSRDRDDLFV
TYTSFLEPSTLYSVNAETLKRDSLKAMPAQFDASKFEAKQYFATSKDGTKVPYFAVMAKDIKLDSTNPTLLYGYGGFEVS
LRPFYSATTGKNWLEQGGVYVLANIRGGGEYGPGWHQAALKENRHKAYEDFEAIAEDLIKRKITSPKHLGIQGGSNGGLL
MGAAFTRRPDLYNAVVCQVPLLDMKRYNKLLAGASWMGEYGNPDIAAEWDYIKTFSPYHNLKKDVEYPKVFFTTSTRDDR
VHPGHARKMVAKMEDMGIEVLYFENMEGGHAGAADNKQTAELNSLAYAYLLKQLKL
>Mature_696_residues
MNKRILLLGAASLLAFNAVAQGDPFIWLEDVEGEKALAWVKTQNDRSLAELKAVPGYNALVDNALDILNDKARIPYASRI
GDHLYNFWKDEANPRGIYRRTTMAEYVKDAPKWETVLDIDALGKSEGVNWVFKGMDCQYPKNIRCLVSLSRGGADAVEIR
EFDLSTLSFVPADKQGFFLPEAKSSTSWIDENTLFVGTDFDEGDAWTDSGYPRKVKVWKRGTDLKDAKEIYAGNKASVAA
SGYVMWDDKTPLQLVSEAETFYEASYKALLDGKLVELPLPKDAELKGFFKGDIFVELKSKLEQGNNSFPQGAIVYTKADK
LLAGTPEFALFVKPDANSSISQVTFSRNAVLVNWLEDVKSKLVRYHKDAKGQWQGEDVGFPSNGSISVFDSSRDRDDLFV
TYTSFLEPSTLYSVNAETLKRDSLKAMPAQFDASKFEAKQYFATSKDGTKVPYFAVMAKDIKLDSTNPTLLYGYGGFEVS
LRPFYSATTGKNWLEQGGVYVLANIRGGGEYGPGWHQAALKENRHKAYEDFEAIAEDLIKRKITSPKHLGIQGGSNGGLL
MGAAFTRRPDLYNAVVCQVPLLDMKRYNKLLAGASWMGEYGNPDIAAEWDYIKTFSPYHNLKKDVEYPKVFFTTSTRDDR
VHPGHARKMVAKMEDMGIEVLYFENMEGGHAGAADNKQTAELNSLAYAYLLKQLKL

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=370, Percent_Identity=34.8648648648649, Blast_Score=232, Evalue=1e-60,
Organism=Homo sapiens, GI108860692, Length=300, Percent_Identity=26.3333333333333, Blast_Score=84, Evalue=6e-16,
Organism=Homo sapiens, GI284172438, Length=205, Percent_Identity=28.2926829268293, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI284172431, Length=205, Percent_Identity=28.2926829268293, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI284172420, Length=205, Percent_Identity=28.2926829268293, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI284172413, Length=205, Percent_Identity=28.2926829268293, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI70778815, Length=205, Percent_Identity=28.2926829268293, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI108860686, Length=205, Percent_Identity=28.2926829268293, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1788150, Length=299, Percent_Identity=29.7658862876254, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24583414, Length=320, Percent_Identity=36.25, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI221510989, Length=283, Percent_Identity=36.7491166077738, Blast_Score=185, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 77686; Mature: 77686

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRILLLGAASLLAFNAVAQGDPFIWLEDVEGEKALAWVKTQNDRSLAELKAVPGYNAL
CCCEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCHHHHHCCCCCHHH
VDNALDILNDKARIPYASRIGDHLYNFWKDEANPRGIYRRTTMAEYVKDAPKWETVLDID
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCEEEEEE
ALGKSEGVNWVFKGMDCQYPKNIRCLVSLSRGGADAVEIREFDLSTLSFVPADKQGFFLP
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEEEECCCCCCCCCCC
EAKSSTSWIDENTLFVGTDFDEGDAWTDSGYPRKVKVWKRGTDLKDAKEIYAGNKASVAA
CCCCCCCEECCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEE
SGYVMWDDKTPLQLVSEAETFYEASYKALLDGKLVELPLPKDAELKGFFKGDIFVELKSK
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCEEEEEHHH
LEQGNNSFPQGAIVYTKADKLLAGTPEFALFVKPDANSSISQVTFSRNAVLVNWLEDVKS
HHCCCCCCCCCEEEEEECCCEEECCCCEEEEECCCCCCCHHEEEECCCEEEEHHHHHHHH
KLVRYHKDAKGQWQGEDVGFPSNGSISVFDSSRDRDDLFVTYTSFLEPSTLYSVNAETLK
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEHHCCCCCEEECCHHHHH
RDSLKAMPAQFDASKFEAKQYFATSKDGTKVPYFAVMAKDIKLDSTNPTLLYGYGGFEVS
HHHHHCCCCCCCHHHHHHHHHEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEE
LRPFYSATTGKNWLEQGGVYVLANIRGGGEYGPGWHQAALKENRHKAYEDFEAIAEDLIK
EEEEECCCCCCHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKITSPKHLGIQGGSNGGLLMGAAFTRRPDLYNAVVCQVPLLDMKRYNKLLAGASWMGEY
HHCCCCCEEEEECCCCCCEEEEEHHCCCCCHHCEEEEECCHHHHHHHHHHHHCCHHCCCC
GNPDIAAEWDYIKTFSPYHNLKKDVEYPKVFFTTSTRDDRVHPGHARKMVAKMEDMGIEV
CCCCCEECCCHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEE
LYFENMEGGHAGAADNKQTAELNSLAYAYLLKQLKL
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKRILLLGAASLLAFNAVAQGDPFIWLEDVEGEKALAWVKTQNDRSLAELKAVPGYNAL
CCCEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCHHHHHCCCCCHHH
VDNALDILNDKARIPYASRIGDHLYNFWKDEANPRGIYRRTTMAEYVKDAPKWETVLDID
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCEEEEEE
ALGKSEGVNWVFKGMDCQYPKNIRCLVSLSRGGADAVEIREFDLSTLSFVPADKQGFFLP
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEEEECCCCCCCCCCC
EAKSSTSWIDENTLFVGTDFDEGDAWTDSGYPRKVKVWKRGTDLKDAKEIYAGNKASVAA
CCCCCCCEECCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEE
SGYVMWDDKTPLQLVSEAETFYEASYKALLDGKLVELPLPKDAELKGFFKGDIFVELKSK
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCEEEEEHHH
LEQGNNSFPQGAIVYTKADKLLAGTPEFALFVKPDANSSISQVTFSRNAVLVNWLEDVKS
HHCCCCCCCCCEEEEEECCCEEECCCCEEEEECCCCCCCHHEEEECCCEEEEHHHHHHHH
KLVRYHKDAKGQWQGEDVGFPSNGSISVFDSSRDRDDLFVTYTSFLEPSTLYSVNAETLK
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEHHCCCCCEEECCHHHHH
RDSLKAMPAQFDASKFEAKQYFATSKDGTKVPYFAVMAKDIKLDSTNPTLLYGYGGFEVS
HHHHHCCCCCCCHHHHHHHHHEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEE
LRPFYSATTGKNWLEQGGVYVLANIRGGGEYGPGWHQAALKENRHKAYEDFEAIAEDLIK
EEEEECCCCCCHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKITSPKHLGIQGGSNGGLLMGAAFTRRPDLYNAVVCQVPLLDMKRYNKLLAGASWMGEY
HHCCCCCEEEEECCCCCCEEEEEHHCCCCCHHCEEEEECCHHHHHHHHHHHHCCHHCCCC
GNPDIAAEWDYIKTFSPYHNLKKDVEYPKVFFTTSTRDDRVHPGHARKMVAKMEDMGIEV
CCCCCEECCCHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEE
LYFENMEGGHAGAADNKQTAELNSLAYAYLLKQLKL
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]