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The map label for this gene is rlmL

Identifier: 119774737

GI number: 119774737

Start: 1954713

End: 1956839

Strand: Direct

Name: rlmL

Synonym: Sama_1600

Alternate gene names: 119774737

Gene position: 1954713-1956839 (Clockwise)

Preceding gene: 119774736

Following gene: 119774738

Centisome position: 45.39

GC content: 52.75

Gene sequence:

>2127_bases
ATGTTTAACTTTTTTGCAGCCGCCCCAAAGGGCTTTGAATATGCACTTGCCAAAGAATTGGAAACCCTTGGCGCAGAGAA
CGTGCGCGAAAGCGTCGCCGGCGTGTATTTCAGTGCCTCGTTGGCGCAAGGCTACCAGATTACCCTGTGGACACGTTTGG
CCAGCCGTATCGTTCTGATCCTTTTCACCGGTGAGTGCCAGAGCGCTGAGCAACTCTACAATGCGGCTTACACCATTGAT
TGGCCATCGCACTTCAGCAATCGCAGTACCTTCAGCATCGATTTTCACGGTACCGGCGGCTTTATCAATAACAGCCAGTT
TGGTGCACTGAAAATCAAAGATGCTGTAGTCGACAGATTCCGTGATGACGATTTAAGTCGCCCGGATGTGGTTAAAGGCC
ATCCGGACATGCGTATCGACGCCCACTATGGCCGCGGTAAAATTACCATCGGGATTAACTTTTCCGGCGCGGCGCTGCAC
CAGCGTGGCTATCGCGGCAATACCGGCGAAGCGCCATTGAAAGAAAACCTTGCCGCCAACATGTTGTATCGCAGTGGTTG
GGCCGACAATCCGGTTACCTTGCTGGACCCTTTCTGCGGCAGTGGCACTGTGCTGATTGAGGCTGCATTGATGGCCTGTG
ATATTGCGCCGGGCCTGATGCGAGAACGATTTGGCTTTGAACACTGGCGCAGACACGACCAGGCCCTCTGGCAACAGGTA
ATCGAGGAAGCCAAGGCCCGTGCTTCTCTGGGTAAAACCCGATGTCAGCTTAAGTTTTATGGCTCGGATATAGACTCGCG
GGTTGTAGCGCTTGCCAAACGCAATGCCAACAGTGCCGGTGTGTTTGACTTTATCGACTTTAAAGTGGCCAATGCCCTTA
ATCTGGAGCCGCCGGTGGCAGAGGGTATGGTGTTGACCAACCCGCCTTATGGTGAGCGCCTGGGGAATGTCACCAGCTTG
CTGCAGCTCTACTTCCAGCTTGGTGAAAAGTTCAAGCAGAGCTACGGTGGCTGGAAGCTCGGTTTGCTTTGCAGCGATAT
GGAGTTGGTATCTTCGCTAAAGCTCAAAGCCGACAAACAGATGAAGATGTACAACGGGGCTCTGGAATGCGCCTTTAATC
TGTATACCCTGCATGCCACCAGTACCCGTCGTGATATTCCTGTGGATACCACGGGCCAGGGCGGCGAGATTGCCGCGCCT
TTTGCCAATCGTTTGAAGAAAAACCTCAAGCAGCTGGAAAAATGGGCCAAGCGAGAAGGCATCGACAGTTACCGTCTGTA
CGACGCCGATATCCCCGAATACAACGTCGCCGTCGACCGATACCTGGATTACGTGGTAGTGCAGGAATATGCGGCGCCTT
CAGCCATTCCTGAAGCTGTCACCAAAAGACGCCTCACCGATGTGCTGCTCGCCTTGCCACGTGCGCTTGGTATAGACCCG
GACAAGATAGTGCTTAAAACCCGTGAGCGTCAGAAGGGCACCAATCAGTACCAAAAATTGGATGGTGAAAAGCTTGAGCT
TGTGACCACCGAGTATGGCTGCAGCTTCAAGCTGAACCTCACCGACTATCTGGACACTGGACTGTTTCTCGACCACCGAC
TGACCCGTAAACTGGTGGGAGAAAAGGCCAAAAACAGAGATGTGCTTAACCTCTTTGCCTATACGGGCAGCGCGTCTGTG
CACGCCGGTAAGGGCGGTGCCAAATCTGTCACTACAGTGGATATGTCCAATACCTATCTCAACTGGGCCAAAGACAACTT
TATGTTGAACGGCCTGATTGGACGCCAGTATCAGTTTGAACAGGCAGACTGCCTACAGTGGATCCGTGATTGCGAAAGGC
AGTTTGACCTGATTTTTATCGACCCGCCCACCTTCTCAAACTCGAAGAGGATGGAAGACTCTTTCGATGTACAGCGTGAC
CACGTGGATTTGCTGGCATCCCTGAAAAAGCTGTTGCGTCCGGGGGGGGAAATTGTGTTCTCCAACAATAAACGCAAGTT
CAAAATGGATATGGAAGCCCTCGCGGCGGCTGGCCTCAAGGCGGTAAACATTGACGATAAGGTGTTGCCAATGGACTACG
CCCGTAATCCGCAAATCCATAACTGCTGGGTTGTGACCCATGGCTGA

Upstream 100 bases:

>100_bases
CGTGTGGTCAAAAAGGCGGCGCAAATCCAAGATTGCTTATCTTTTAAAGTGTCGAGCGGGTATAATATGCCCTTTGATTT
TTCTGCAGGCGTAACTCCCC

Downstream 100 bases:

>100_bases
GTTTGTGTTGTACCACACCGATGGTTGTCATCTTTGCCATTTGGCCGAGGCGCTGCTGCAAGAGGCCGGCGTGACCTACC
TGATGGTGGATATTTGTGAT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MFNFFAAAPKGFEYALAKELETLGAENVRESVAGVYFSASLAQGYQITLWTRLASRIVLILFTGECQSAEQLYNAAYTID
WPSHFSNRSTFSIDFHGTGGFINNSQFGALKIKDAVVDRFRDDDLSRPDVVKGHPDMRIDAHYGRGKITIGINFSGAALH
QRGYRGNTGEAPLKENLAANMLYRSGWADNPVTLLDPFCGSGTVLIEAALMACDIAPGLMRERFGFEHWRRHDQALWQQV
IEEAKARASLGKTRCQLKFYGSDIDSRVVALAKRNANSAGVFDFIDFKVANALNLEPPVAEGMVLTNPPYGERLGNVTSL
LQLYFQLGEKFKQSYGGWKLGLLCSDMELVSSLKLKADKQMKMYNGALECAFNLYTLHATSTRRDIPVDTTGQGGEIAAP
FANRLKKNLKQLEKWAKREGIDSYRLYDADIPEYNVAVDRYLDYVVVQEYAAPSAIPEAVTKRRLTDVLLALPRALGIDP
DKIVLKTRERQKGTNQYQKLDGEKLELVTTEYGCSFKLNLTDYLDTGLFLDHRLTRKLVGEKAKNRDVLNLFAYTGSASV
HAGKGGAKSVTTVDMSNTYLNWAKDNFMLNGLIGRQYQFEQADCLQWIRDCERQFDLIFIDPPTFSNSKRMEDSFDVQRD
HVDLLASLKKLLRPGGEIVFSNNKRKFKMDMEALAAAGLKAVNIDDKVLPMDYARNPQIHNCWVVTHG

Sequences:

>Translated_708_residues
MFNFFAAAPKGFEYALAKELETLGAENVRESVAGVYFSASLAQGYQITLWTRLASRIVLILFTGECQSAEQLYNAAYTID
WPSHFSNRSTFSIDFHGTGGFINNSQFGALKIKDAVVDRFRDDDLSRPDVVKGHPDMRIDAHYGRGKITIGINFSGAALH
QRGYRGNTGEAPLKENLAANMLYRSGWADNPVTLLDPFCGSGTVLIEAALMACDIAPGLMRERFGFEHWRRHDQALWQQV
IEEAKARASLGKTRCQLKFYGSDIDSRVVALAKRNANSAGVFDFIDFKVANALNLEPPVAEGMVLTNPPYGERLGNVTSL
LQLYFQLGEKFKQSYGGWKLGLLCSDMELVSSLKLKADKQMKMYNGALECAFNLYTLHATSTRRDIPVDTTGQGGEIAAP
FANRLKKNLKQLEKWAKREGIDSYRLYDADIPEYNVAVDRYLDYVVVQEYAAPSAIPEAVTKRRLTDVLLALPRALGIDP
DKIVLKTRERQKGTNQYQKLDGEKLELVTTEYGCSFKLNLTDYLDTGLFLDHRLTRKLVGEKAKNRDVLNLFAYTGSASV
HAGKGGAKSVTTVDMSNTYLNWAKDNFMLNGLIGRQYQFEQADCLQWIRDCERQFDLIFIDPPTFSNSKRMEDSFDVQRD
HVDLLASLKKLLRPGGEIVFSNNKRKFKMDMEALAAAGLKAVNIDDKVLPMDYARNPQIHNCWVVTHG
>Mature_708_residues
MFNFFAAAPKGFEYALAKELETLGAENVRESVAGVYFSASLAQGYQITLWTRLASRIVLILFTGECQSAEQLYNAAYTID
WPSHFSNRSTFSIDFHGTGGFINNSQFGALKIKDAVVDRFRDDDLSRPDVVKGHPDMRIDAHYGRGKITIGINFSGAALH
QRGYRGNTGEAPLKENLAANMLYRSGWADNPVTLLDPFCGSGTVLIEAALMACDIAPGLMRERFGFEHWRRHDQALWQQV
IEEAKARASLGKTRCQLKFYGSDIDSRVVALAKRNANSAGVFDFIDFKVANALNLEPPVAEGMVLTNPPYGERLGNVTSL
LQLYFQLGEKFKQSYGGWKLGLLCSDMELVSSLKLKADKQMKMYNGALECAFNLYTLHATSTRRDIPVDTTGQGGEIAAP
FANRLKKNLKQLEKWAKREGIDSYRLYDADIPEYNVAVDRYLDYVVVQEYAAPSAIPEAVTKRRLTDVLLALPRALGIDP
DKIVLKTRERQKGTNQYQKLDGEKLELVTTEYGCSFKLNLTDYLDTGLFLDHRLTRKLVGEKAKNRDVLNLFAYTGSASV
HAGKGGAKSVTTVDMSNTYLNWAKDNFMLNGLIGRQYQFEQADCLQWIRDCERQFDLIFIDPPTFSNSKRMEDSFDVQRD
HVDLLASLKKLLRPGGEIVFSNNKRKFKMDMEALAAAGLKAVNIDDKVLPMDYARNPQIHNCWVVTHG

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=713, Percent_Identity=52.7349228611501, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=236, Percent_Identity=28.8135593220339, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_SHEAM (A1S601)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927477.1
- ProteinModelPortal:   A1S601
- SMR:   A1S601
- STRING:   A1S601
- GeneID:   4605959
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1600
- NMPDR:   fig|326297.7.peg.1512
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- PhylomeDB:   A1S601
- ProtClustDB:   PRK11783
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 79420; Mature: 79420

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNFFAAAPKGFEYALAKELETLGAENVRESVAGVYFSASLAQGYQITLWTRLASRIVLI
CCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHEEEHHHCCCCEEEHHHHHHCCEEEE
LFTGECQSAEQLYNAAYTIDWPSHFSNRSTFSIDFHGTGGFINNSQFGALKIKDAVVDRF
EEECCCCCHHHHHCCEEEEECCHHCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHH
RDDDLSRPDVVKGHPDMRIDAHYGRGKITIGINFSGAALHQRGYRGNTGEAPLKENLAAN
CCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHH
MLYRSGWADNPVTLLDPFCGSGTVLIEAALMACDIAPGLMRERFGFEHWRRHDQALWQQV
HHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHH
IEEAKARASLGKTRCQLKFYGSDIDSRVVALAKRNANSAGVFDFIDFKVANALNLEPPVA
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEEHHHHHHCCCCCCCCHH
EGMVLTNPPYGERLGNVTSLLQLYFQLGEKFKQSYGGWKLGLLCSDMELVSSLKLKADKQ
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCHH
MKMYNGALECAFNLYTLHATSTRRDIPVDTTGQGGEIAAPFANRLKKNLKQLEKWAKREG
HHHHCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCC
IDSYRLYDADIPEYNVAVDRYLDYVVVQEYAAPSAIPEAVTKRRLTDVLLALPRALGIDP
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DKIVLKTRERQKGTNQYQKLDGEKLELVTTEYGCSFKLNLTDYLDTGLFLDHRLTRKLVG
CCEEEEEHHHHCCCCHHHHCCCCEEEEEEEECCCEEEEECHHHHCCCHHHHHHHHHHHHC
EKAKNRDVLNLFAYTGSASVHAGKGGAKSVTTVDMSNTYLNWAKDNFMLNGLIGRQYQFE
CCCCCCCEEEEEEECCCCEEECCCCCCCCEEEEECCCCEEEECCCCEEEECEECCCCCCC
QADCLQWIRDCERQFDLIFIDPPTFSNSKRMEDSFDVQRDHVDLLASLKKLLRPGGEIVF
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
SNNKRKFKMDMEALAAAGLKAVNIDDKVLPMDYARNPQIHNCWVVTHG
CCCCEEEEHHHHHHHHCCCEEECCCCCEECCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MFNFFAAAPKGFEYALAKELETLGAENVRESVAGVYFSASLAQGYQITLWTRLASRIVLI
CCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHEEEHHHCCCCEEEHHHHHHCCEEEE
LFTGECQSAEQLYNAAYTIDWPSHFSNRSTFSIDFHGTGGFINNSQFGALKIKDAVVDRF
EEECCCCCHHHHHCCEEEEECCHHCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHH
RDDDLSRPDVVKGHPDMRIDAHYGRGKITIGINFSGAALHQRGYRGNTGEAPLKENLAAN
CCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHH
MLYRSGWADNPVTLLDPFCGSGTVLIEAALMACDIAPGLMRERFGFEHWRRHDQALWQQV
HHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHH
IEEAKARASLGKTRCQLKFYGSDIDSRVVALAKRNANSAGVFDFIDFKVANALNLEPPVA
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEEHHHHHHCCCCCCCCHH
EGMVLTNPPYGERLGNVTSLLQLYFQLGEKFKQSYGGWKLGLLCSDMELVSSLKLKADKQ
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCHH
MKMYNGALECAFNLYTLHATSTRRDIPVDTTGQGGEIAAPFANRLKKNLKQLEKWAKREG
HHHHCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCC
IDSYRLYDADIPEYNVAVDRYLDYVVVQEYAAPSAIPEAVTKRRLTDVLLALPRALGIDP
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DKIVLKTRERQKGTNQYQKLDGEKLELVTTEYGCSFKLNLTDYLDTGLFLDHRLTRKLVG
CCEEEEEHHHHCCCCHHHHCCCCEEEEEEEECCCEEEEECHHHHCCCHHHHHHHHHHHHC
EKAKNRDVLNLFAYTGSASVHAGKGGAKSVTTVDMSNTYLNWAKDNFMLNGLIGRQYQFE
CCCCCCCEEEEEEECCCCEEECCCCCCCCEEEEECCCCEEEECCCCEEEECEECCCCCCC
QADCLQWIRDCERQFDLIFIDPPTFSNSKRMEDSFDVQRDHVDLLASLKKLLRPGGEIVF
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
SNNKRKFKMDMEALAAAGLKAVNIDDKVLPMDYARNPQIHNCWVVTHG
CCCCEEEEHHHHHHHHCCCEEECCCCCEECCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA