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The map label for this gene is pflA [H]

Identifier: 119774634

GI number: 119774634

Start: 1811791

End: 1812531

Strand: Reverse

Name: pflA [H]

Synonym: Sama_1497

Alternate gene names: 119774634

Gene position: 1812531-1811791 (Counterclockwise)

Preceding gene: 119774635

Following gene: 119774632

Centisome position: 42.09

GC content: 53.58

Gene sequence:

>741_bases
ATGACAGTCACAGGCAGGGTTCATTCAGTGGAATCCTTCGGCACAGTGGACGGTCCCGGCATCAGGTTTATCACCTTTAT
GCAGGGTTGTCTGATGCGCTGCCAATACTGCCATAACCGTGATACCTGGGATTTGGATGGCGGCAAGGAAATGCAGGTCG
ATGAGCTGATGAGTCAAATCCTCAGCTATCGGCCTTTTTTGGATGCCAGCGGCGGCGGTGTCACTGCCAGTGGCGGTGAG
GCCATATTGCAGGCCGAATTTGTGGCCGACCTGTTCAAGGCCTGCCGCGCCGAAGGCATTCATACCTGTCTGGATACCAA
TGGTTTCGTGCGAAAATACACGCCCGCCATTGATGTACTCTTAGACAACACCGATCTGGTGCTCTTGGATATCAAACACA
TAGATGATGCCAAACATCAGGATTTAACCAAGGTCAGCAATCATCGCACCCTGCAGTTTGCCGAATACCTCGCCAAACGC
GACCAGAAAACCTGGATCCGTTACGTGGTAGTCGCGGGCTTTACCGAAGATGTAGAAAGTGCTGAAAAACTGGCTGATTT
TATCGCCCCCATGGGCAACGTGGAAAAGGTGGAACTGCTGCCCTACCACGAGCTGGGCAAGCACAAGTGGGAAGCCATGG
GTGAGGATTATCCGCTCAAGGGTGTTCATCCCCCCACCGCCGACACCATGGAAGCCATCAAGGCCGTGTTTACCCGCCGC
GGACTGACCGCGACTTATTAA

Upstream 100 bases:

>100_bases
TCAAGGTTTGTAATTCAGGCGCTGGTAATTCATCGGTCGCTAAAGGACAATTGTGGCTTGCCACAATTGTCCTCAGTTTC
AGCCAACCCCAGGAGTTTCA

Downstream 100 bases:

>100_bases
GACTGTTACCGCGGAATACTCGCAGTTAAAAAGGTGCTCGTTGAGCCGGAATGATGCTCAAAGCTCGTCCCAGTAACTTG
CATCGAGTCGGTCGAAGGCT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTVTGRVHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEMQVDELMSQILSYRPFLDASGGGVTASGGE
AILQAEFVADLFKACRAEGIHTCLDTNGFVRKYTPAIDVLLDNTDLVLLDIKHIDDAKHQDLTKVSNHRTLQFAEYLAKR
DQKTWIRYVVVAGFTEDVESAEKLADFIAPMGNVEKVELLPYHELGKHKWEAMGEDYPLKGVHPPTADTMEAIKAVFTRR
GLTATY

Sequences:

>Translated_246_residues
MTVTGRVHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEMQVDELMSQILSYRPFLDASGGGVTASGGE
AILQAEFVADLFKACRAEGIHTCLDTNGFVRKYTPAIDVLLDNTDLVLLDIKHIDDAKHQDLTKVSNHRTLQFAEYLAKR
DQKTWIRYVVVAGFTEDVESAEKLADFIAPMGNVEKVELLPYHELGKHKWEAMGEDYPLKGVHPPTADTMEAIKAVFTRR
GLTATY
>Mature_245_residues
TVTGRVHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEMQVDELMSQILSYRPFLDASGGGVTASGGEA
ILQAEFVADLFKACRAEGIHTCLDTNGFVRKYTPAIDVLLDNTDLVLLDIKHIDDAKHQDLTKVSNHRTLQFAEYLAKRD
QKTWIRYVVVAGFTEDVESAEKLADFIAPMGNVEKVELLPYHELGKHKWEAMGEDYPLKGVHPPTADTMEAIKAVFTRRG
LTATY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=241, Percent_Identity=67.2199170124481, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=26.2773722627737, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI226510931, Length=300, Percent_Identity=28.6666666666667, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790839, Length=249, Percent_Identity=29.3172690763052, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27508; Mature: 27376

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00018 EF_HAND_1 ; PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTGRVHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEMQVDELMSQI
CCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LSYRPFLDASGGGVTASGGEAILQAEFVADLFKACRAEGIHTCLDTNGFVRKYTPAIDVL
HHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCEEEEE
LDNTDLVLLDIKHIDDAKHQDLTKVSNHRTLQFAEYLAKRDQKTWIRYVVVAGFTEDVES
ECCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHEEECCHHHHHH
AEKLADFIAPMGNVEKVELLPYHELGKHKWEAMGEDYPLKGVHPPTADTMEAIKAVFTRR
HHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATY
CCCCCC
>Mature Secondary Structure 
TVTGRVHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEMQVDELMSQI
CCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LSYRPFLDASGGGVTASGGEAILQAEFVADLFKACRAEGIHTCLDTNGFVRKYTPAIDVL
HHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCEEEEE
LDNTDLVLLDIKHIDDAKHQDLTKVSNHRTLQFAEYLAKRDQKTWIRYVVVAGFTEDVES
ECCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHEEECCHHHHHH
AEKLADFIAPMGNVEKVELLPYHELGKHKWEAMGEDYPLKGVHPPTADTMEAIKAVFTRR
HHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]