Click here to switch to the map view.

The map label for this gene is pflB [H]

Identifier: 119774635

GI number: 119774635

Start: 1812619

End: 1814901

Strand: Reverse

Name: pflB [H]

Synonym: Sama_1498

Alternate gene names: 119774635

Gene position: 1814901-1812619 (Counterclockwise)

Preceding gene: 119774636

Following gene: 119774634

Centisome position: 42.15

GC content: 55.28

Gene sequence:

>2283_bases
ATGACCGATAAAACTGAGCTGTTCCAAACCGCGTGGGAAGGATTCGTTCCCGGTGATTGGAAGTCCGACGTCAATGTACG
TGACTTTATTCAGACCAACTACACCCCTTATGAAGGTGACGACAGCTTCCTGGCCGGCCCCACCGCCGCTACCAACGCCC
TGTGGGCCAAGGTGATGGAAGGTATCAAGGAAGAAAACCGCACCCACGCGCCGCTGGACTTTGATACCGACAAGGTATCG
ACCATCACTTCCCACGATGCCGGTTATATCGAAAAAGAGCTGGAAACCATTGTTGGTCTGCAAACCGATGCGCCACTCAA
GCGCGCCATGCTGCCAAACGGCGGTATCCGTATGGTGGAAAGCTCCTGCGCCGCCTACAACCGTGAACTGAATGCCGACA
TCAAGTATGTGTATTCTGAGCTGCGCAAGACCCACAACCAGGGCGTATTCGACATCTATACCCCGGAAATCCTTGCCTGC
CGTAAATCCGGCGTGCTGACCGGCCTGCCCGATGCCTATGGCCGTGGCCGTATCATTGGTGACTACCGCCGCGTGGCCCT
GTACGGTATCGATTACCTGATGGCCGATAAACTGAAGCAATTCACCTCGCTGCAATCCCGTTTTGAAGAAGGTGAAGATC
TGCAGGCCACCATGCAGCTACGCGAAGAAATCGCCGAGCAGCACCGCGCTCTGGCCCAGATGAAGAAAATGGCCGCGAAA
TACGGCTACGACATTTCTGGCCCCGCCAAAAACGCCCAGGAAGCCATTCAGTGGACCTACTTTGGCTACCTTGCTGCGGT
GAAGAGCCAAAACGGTGCCGCCATGTCCCTTGGCCGTACTTCCAGCTTTATCGACATCTACATTGAGCGTGACCTGAAAA
ACGGTACTCTGACCGAAGAGCAGGCGCAGGAGATGATTGACCATTTCGTGATGAAGCTGCGCATGGTGCGCTTCCTGCGT
ACCCCTGAGTACGATGAACTGTTCTCCGGCGACCCAATCTGGGCCACCGAATCAATCGGCGGTATGGGTCTTGATGGCCG
TACTCTGGTGACCAAGTCCAGCTTCCGCTTCCTGCATACCCTGTACAACATGGGCCCAAGCCCAGAGCCAAACATCACTG
TGCTCTGGTCTGAAAAGCTGCCAGAAAACTTCAAAACCTTCTGTGCCAAGGTGTCCATCGACACCAGCTCCATCCAGTAC
GAAAACGATGACCTGATGCGTCCGGACTTCGAGTCTGACGACTATGCCATCGCCTGCTGCGTCAGCCCCATGGTGGTGGG
TAAGCACATGCAGTTCTTCGGTGCCCGTGCCAACCTCGCCAAGACCATGCTGTATGCCATCAACGGTGGCGTAGACGAAA
AGCTGAAAGTGCAGATAGGTCCTAAATCTGAGCCTATCACCAGCGAGTACCTGGACTATGACGATGTCATGAGTCGTCTG
GATAACCTGATGGAGTGGCTGGCGACCCAGTATGTCTCTGCGCTGAACGCCATTCACTTTATGCACGACAAGTATTCTTA
CGAAGCCGCGCTGATGGCGCTGCACGACCGCGACGTGCGCCGCACCATGGCCTGTGGTATCGCCGGTCTGTCCATTGCTG
CCGACTCCCTGTCTGCCATCAAGTACGCCAAGGTGAAGCCGGTACGCGACGAGCACGGCATCGCCGTTGACTTCGAAATT
GAGGGTGACTATCCCAAGTTCGGTAACAACGATGCCCGCGTGGATGATATCGCCTGTATGCTGGTTGAAGGCTTTATGGC
CAAAATTCGCAACAAGAAGATGTACCGCAACGCCATCCCAACCCAGTCTATCCTGACCATCACCTCTAACGTGGTGTACG
GCAAGAAGACAGGTAACACCCCCGATGGCCGCCGCGCCGGAGCACCTTTCGCCCCGGGTGCCAACCCAATGCACGGCCGT
GATGAGAAAGGCGCCATCGCCTCACTCACTTCCGTGGCCAAGCTGCCCTTTGCCCACGCACAGGATGGTATTTCTTACAC
CTTCTCTATCGTTCCCAATGCTCTGGGTAAAGATGAAAAGGCCCGTGAGCGTAACCTGGCAGCCCTGATGGACGGTTATT
TCCGCCACAGTGAAGGCCGTGAAGGTGGCCAACACCTGAACGTGAACGTGATGAACCGTGAAATGCTTGAGGATGCCATC
GCAAACCCTGACAAGTATCCACAGCTCACCATCCGTGTATCCGGTTATGCAGTGCGCTTCAACTCACTGACACCAGAGCA
ACAGCAGGATGTGATCACCCGCACCTTTACTCAAGGTTTGTAA

Upstream 100 bases:

>100_bases
CCGGGGTCCTGGCTTCATCAGGGGATGTTTCGCCGGGATGTAAAAAGACTCGCAAGGGGAAACATCCCCAACCCTTTTCT
CAAATAGAAGGTGAAGTACG

Downstream 100 bases:

>100_bases
TTCAGGCGCTGGTAATTCATCGGTCGCTAAAGGACAATTGTGGCTTGCCACAATTGTCCTCAGTTTCAGCCAACCCCAGG
AGTTTCAATGACAGTCACAG

Product: formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MTDKTELFQTAWEGFVPGDWKSDVNVRDFIQTNYTPYEGDDSFLAGPTAATNALWAKVMEGIKEENRTHAPLDFDTDKVS
TITSHDAGYIEKELETIVGLQTDAPLKRAMLPNGGIRMVESSCAAYNRELNADIKYVYSELRKTHNQGVFDIYTPEILAC
RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMADKLKQFTSLQSRFEEGEDLQATMQLREEIAEQHRALAQMKKMAAK
YGYDISGPAKNAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFIDIYIERDLKNGTLTEEQAQEMIDHFVMKLRMVRFLR
TPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLHTLYNMGPSPEPNITVLWSEKLPENFKTFCAKVSIDTSSIQY
ENDDLMRPDFESDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKVQIGPKSEPITSEYLDYDDVMSRL
DNLMEWLATQYVSALNAIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKYAKVKPVRDEHGIAVDFEI
EGDYPKFGNNDARVDDIACMLVEGFMAKIRNKKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGR
DEKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDEKARERNLAALMDGYFRHSEGREGGQHLNVNVMNREMLEDAI
ANPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTQGL

Sequences:

>Translated_760_residues
MTDKTELFQTAWEGFVPGDWKSDVNVRDFIQTNYTPYEGDDSFLAGPTAATNALWAKVMEGIKEENRTHAPLDFDTDKVS
TITSHDAGYIEKELETIVGLQTDAPLKRAMLPNGGIRMVESSCAAYNRELNADIKYVYSELRKTHNQGVFDIYTPEILAC
RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMADKLKQFTSLQSRFEEGEDLQATMQLREEIAEQHRALAQMKKMAAK
YGYDISGPAKNAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFIDIYIERDLKNGTLTEEQAQEMIDHFVMKLRMVRFLR
TPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLHTLYNMGPSPEPNITVLWSEKLPENFKTFCAKVSIDTSSIQY
ENDDLMRPDFESDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKVQIGPKSEPITSEYLDYDDVMSRL
DNLMEWLATQYVSALNAIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKYAKVKPVRDEHGIAVDFEI
EGDYPKFGNNDARVDDIACMLVEGFMAKIRNKKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGR
DEKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDEKARERNLAALMDGYFRHSEGREGGQHLNVNVMNREMLEDAI
ANPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTQGL
>Mature_759_residues
TDKTELFQTAWEGFVPGDWKSDVNVRDFIQTNYTPYEGDDSFLAGPTAATNALWAKVMEGIKEENRTHAPLDFDTDKVST
ITSHDAGYIEKELETIVGLQTDAPLKRAMLPNGGIRMVESSCAAYNRELNADIKYVYSELRKTHNQGVFDIYTPEILACR
KSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMADKLKQFTSLQSRFEEGEDLQATMQLREEIAEQHRALAQMKKMAAKY
GYDISGPAKNAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFIDIYIERDLKNGTLTEEQAQEMIDHFVMKLRMVRFLRT
PEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLHTLYNMGPSPEPNITVLWSEKLPENFKTFCAKVSIDTSSIQYE
NDDLMRPDFESDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKVQIGPKSEPITSEYLDYDDVMSRLD
NLMEWLATQYVSALNAIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKYAKVKPVRDEHGIAVDFEIE
GDYPKFGNNDARVDDIACMLVEGFMAKIRNKKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRD
EKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDEKARERNLAALMDGYFRHSEGREGGQHLNVNVMNREMLEDAIA
NPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTQGL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=80.3947368421053, Blast_Score=1313, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=755, Percent_Identity=74.4370860927152, Blast_Score=1216, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=566, Percent_Identity=27.3851590106007, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1790388, Length=671, Percent_Identity=24.7391952309985, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=81.6666666666667, Blast_Score=110, Evalue=5e-25,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85021; Mature: 84890

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTELFQTAWEGFVPGDWKSDVNVRDFIQTNYTPYEGDDSFLAGPTAATNALWAKVME
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHH
GIKEENRTHAPLDFDTDKVSTITSHDAGYIEKELETIVGLQTDAPLKRAMLPNGGIRMVE
HHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHH
SSCAAYNRELNADIKYVYSELRKTHNQGVFDIYTPEILACRKSGVLTGLPDAYGRGRIIG
HHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEH
DYRRVALYGIDYLMADKLKQFTSLQSRFEEGEDLQATMQLREEIAEQHRALAQMKKMAAK
HHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGYDISGPAKNAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFIDIYIERDLKNGTLTEE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCCCHH
QAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLHT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHH
LYNMGPSPEPNITVLWSEKLPENFKTFCAKVSIDTSSIQYENDDLMRPDFESDDYAIACC
HHHCCCCCCCCEEEEECCCCCHHHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKVQIGPKSEPITSEYLDYDDVMSRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHH
DNLMEWLATQYVSALNAIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYAKVKPVRDEHGIAVDFEIEGDYPKFGNNDARVDDIACMLVEGFMAKIRNKKMYRNAIP
HHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
CHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHC
QDGISYTFSIVPNALGKDEKARERNLAALMDGYFRHSEGREGGQHLNVNVMNREMLEDAI
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHH
ANPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTQGL
CCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDKTELFQTAWEGFVPGDWKSDVNVRDFIQTNYTPYEGDDSFLAGPTAATNALWAKVME
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHH
GIKEENRTHAPLDFDTDKVSTITSHDAGYIEKELETIVGLQTDAPLKRAMLPNGGIRMVE
HHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHH
SSCAAYNRELNADIKYVYSELRKTHNQGVFDIYTPEILACRKSGVLTGLPDAYGRGRIIG
HHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEH
DYRRVALYGIDYLMADKLKQFTSLQSRFEEGEDLQATMQLREEIAEQHRALAQMKKMAAK
HHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGYDISGPAKNAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFIDIYIERDLKNGTLTEE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCCCHH
QAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLHT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHH
LYNMGPSPEPNITVLWSEKLPENFKTFCAKVSIDTSSIQYENDDLMRPDFESDDYAIACC
HHHCCCCCCCCEEEEECCCCCHHHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKVQIGPKSEPITSEYLDYDDVMSRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHH
DNLMEWLATQYVSALNAIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYAKVKPVRDEHGIAVDFEIEGDYPKFGNNDARVDDIACMLVEGFMAKIRNKKMYRNAIP
HHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
CHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHC
QDGISYTFSIVPNALGKDEKARERNLAALMDGYFRHSEGREGGQHLNVNVMNREMLEDAI
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHH
ANPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTQGL
CCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]